Amino acid dipepetide frequency for Duganella sp. Leaf126

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.642AlaAla: 20.642 ± 0.221	1.185AlaCys: 1.185 ± 0.032	7.481AlaAsp: 7.481 ± 0.075	5.958AlaGlu: 5.958 ± 0.08	3.698AlaPhe: 3.698 ± 0.05	11.607AlaGly: 11.607 ± 0.149	2.657AlaHis: 2.657 ± 0.047	5.937AlaIle: 5.937 ± 0.069	3.74AlaLys: 3.74 ± 0.08	14.446AlaLeu: 14.446 ± 0.133	3.496AlaMet: 3.496 ± 0.058	3.423AlaAsn: 3.423 ± 0.06	7.106AlaPro: 7.106 ± 0.107	5.922AlaGln: 5.922 ± 0.079	9.009AlaArg: 9.009 ± 0.095	7.358AlaSer: 7.358 ± 0.09	7.001AlaThr: 7.001 ± 0.084	9.039AlaVal: 9.039 ± 0.083	1.737AlaTrp: 1.737 ± 0.038	2.871AlaTyr: 2.871 ± 0.055	0.003AlaXaa: 0.003 ± 0.001
Cys	1.132CysAla: 1.132 ± 0.028	0.114CysCys: 0.114 ± 0.01	0.499CysAsp: 0.499 ± 0.019	0.359CysGlu: 0.359 ± 0.017	0.266CysPhe: 0.266 ± 0.014	0.846CysGly: 0.846 ± 0.027	0.239CysHis: 0.239 ± 0.013	0.346CysIle: 0.346 ± 0.017	0.228CysLys: 0.228 ± 0.011	0.763CysLeu: 0.763 ± 0.026	0.175CysMet: 0.175 ± 0.011	0.213CysAsn: 0.213 ± 0.012	0.385CysPro: 0.385 ± 0.016	0.26CysGln: 0.26 ± 0.013	0.509CysArg: 0.509 ± 0.02	0.46CysSer: 0.46 ± 0.018	0.449CysThr: 0.449 ± 0.019	0.582CysVal: 0.582 ± 0.021	0.118CysTrp: 0.118 ± 0.008	0.218CysTyr: 0.218 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.453AspAla: 7.453 ± 0.083	0.458AspCys: 0.458 ± 0.019	3.288AspAsp: 3.288 ± 0.047	2.807AspGlu: 2.807 ± 0.059	2.064AspPhe: 2.064 ± 0.046	5.249AspGly: 5.249 ± 0.069	1.223AspHis: 1.223 ± 0.031	2.821AspIle: 2.821 ± 0.04	2.067AspLys: 2.067 ± 0.041	5.508AspLeu: 5.508 ± 0.063	1.312AspMet: 1.312 ± 0.032	1.646AspAsn: 1.646 ± 0.037	2.844AspPro: 2.844 ± 0.048	2.184AspGln: 2.184 ± 0.042	3.321AspArg: 3.321 ± 0.054	2.582AspSer: 2.582 ± 0.042	2.973AspThr: 2.973 ± 0.044	4.113AspVal: 4.113 ± 0.051	0.917AspTrp: 0.917 ± 0.024	1.667AspTyr: 1.667 ± 0.035	0.001AspXaa: 0.001 ± 0.001
Glu	5.752GluAla: 5.752 ± 0.07	0.295GluCys: 0.295 ± 0.015	1.979GluAsp: 1.979 ± 0.038	2.312GluGlu: 2.312 ± 0.056	1.62GluPhe: 1.62 ± 0.037	2.895GluGly: 2.895 ± 0.05	1.265GluHis: 1.265 ± 0.028	2.544GluIle: 2.544 ± 0.049	1.855GluLys: 1.855 ± 0.046	5.385GluLeu: 5.385 ± 0.072	1.165GluMet: 1.165 ± 0.025	1.331GluAsn: 1.331 ± 0.031	1.971GluPro: 1.971 ± 0.04	2.869GluGln: 2.869 ± 0.052	3.965GluArg: 3.965 ± 0.065	2.107GluSer: 2.107 ± 0.042	2.388GluThr: 2.388 ± 0.041	3.394GluVal: 3.394 ± 0.059	0.583GluTrp: 0.583 ± 0.021	1.076GluTyr: 1.076 ± 0.03	0.001GluXaa: 0.001 ± 0.001
Phe	3.994PheAla: 3.994 ± 0.052	0.347PheCys: 0.347 ± 0.017	2.56PheAsp: 2.56 ± 0.043	1.735PheGlu: 1.735 ± 0.04	1.29PhePhe: 1.29 ± 0.032	3.241PheGly: 3.241 ± 0.043	0.696PheHis: 0.696 ± 0.023	1.562PheIle: 1.562 ± 0.031	1.204PheLys: 1.204 ± 0.029	2.739PheLeu: 2.739 ± 0.049	0.798PheMet: 0.798 ± 0.023	1.294PheAsn: 1.294 ± 0.03	1.357PhePro: 1.357 ± 0.031	1.067PheGln: 1.067 ± 0.027	1.778PheArg: 1.778 ± 0.034	2.216PheSer: 2.216 ± 0.044	1.973PheThr: 1.973 ± 0.038	2.453PheVal: 2.453 ± 0.039	0.422PheTrp: 0.422 ± 0.017	0.967PheTyr: 0.967 ± 0.029	0.001PheXaa: 0.001 ± 0.001
Gly	10.43GlyAla: 10.43 ± 0.135	0.723GlyCys: 0.723 ± 0.019	4.254GlyAsp: 4.254 ± 0.059	3.66GlyGlu: 3.66 ± 0.045	3.094GlyPhe: 3.094 ± 0.046	6.717GlyGly: 6.717 ± 0.121	1.811GlyHis: 1.811 ± 0.03	3.876GlyIle: 3.876 ± 0.052	3.738GlyLys: 3.738 ± 0.052	7.834GlyLeu: 7.834 ± 0.076	2.358GlyMet: 2.358 ± 0.043	2.669GlyAsn: 2.669 ± 0.072	2.745GlyPro: 2.745 ± 0.039	3.374GlyGln: 3.374 ± 0.051	4.971GlyArg: 4.971 ± 0.068	4.595GlySer: 4.595 ± 0.087	4.593GlyThr: 4.593 ± 0.079	6.477GlyVal: 6.477 ± 0.075	1.246GlyTrp: 1.246 ± 0.033	2.527GlyTyr: 2.527 ± 0.042	0.002GlyXaa: 0.002 ± 0.001
His	2.97HisAla: 2.97 ± 0.048	0.243HisCys: 0.243 ± 0.014	1.369HisAsp: 1.369 ± 0.036	1.001HisGlu: 1.001 ± 0.026	0.864HisPhe: 0.864 ± 0.023	2.167HisGly: 2.167 ± 0.033	0.662HisHis: 0.662 ± 0.021	0.933HisIle: 0.933 ± 0.027	0.566HisLys: 0.566 ± 0.02	2.18HisLeu: 2.18 ± 0.039	0.477HisMet: 0.477 ± 0.019	0.545HisAsn: 0.545 ± 0.017	1.332HisPro: 1.332 ± 0.033	0.853HisGln: 0.853 ± 0.02	1.366HisArg: 1.366 ± 0.034	1.025HisSer: 1.025 ± 0.027	1.106HisThr: 1.106 ± 0.03	1.418HisVal: 1.418 ± 0.033	0.37HisTrp: 0.37 ± 0.016	0.706HisTyr: 0.706 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.6IleAla: 6.6 ± 0.076	0.397IleCys: 0.397 ± 0.017	3.63IleAsp: 3.63 ± 0.05	2.838IleGlu: 2.838 ± 0.054	1.33IlePhe: 1.33 ± 0.033	4.373IleGly: 4.373 ± 0.055	0.855IleHis: 0.855 ± 0.024	1.85IleIle: 1.85 ± 0.039	1.677IleLys: 1.677 ± 0.041	3.478IleLeu: 3.478 ± 0.056	0.936IleMet: 0.936 ± 0.028	1.681IleAsn: 1.681 ± 0.037	1.911IlePro: 1.911 ± 0.038	1.066IleGln: 1.066 ± 0.027	2.506IleArg: 2.506 ± 0.046	2.58IleSer: 2.58 ± 0.038	2.678IleThr: 2.678 ± 0.043	3.708IleVal: 3.708 ± 0.059	0.458IleTrp: 0.458 ± 0.016	1.038IleTyr: 1.038 ± 0.025	0.001IleXaa: 0.001 ± 0.001
Lys	3.891LysAla: 3.891 ± 0.07	0.153LysCys: 0.153 ± 0.009	1.677LysAsp: 1.677 ± 0.043	1.534LysGlu: 1.534 ± 0.037	1.077LysPhe: 1.077 ± 0.032	2.24LysGly: 2.24 ± 0.044	0.693LysHis: 0.693 ± 0.023	1.718LysIle: 1.718 ± 0.034	1.507LysLys: 1.507 ± 0.043	3.782LysLeu: 3.782 ± 0.064	0.959LysMet: 0.959 ± 0.028	1.132LysAsn: 1.132 ± 0.032	2.087LysPro: 2.087 ± 0.05	1.451LysGln: 1.451 ± 0.038	2.04LysArg: 2.04 ± 0.047	1.841LysSer: 1.841 ± 0.043	2.002LysThr: 2.002 ± 0.041	2.599LysVal: 2.599 ± 0.049	0.344LysTrp: 0.344 ± 0.017	0.745LysTyr: 0.745 ± 0.026	0.001LysXaa: 0.001 ± 0.001
Leu	15.053LeuAla: 15.053 ± 0.125	0.929LeuCys: 0.929 ± 0.024	6.119LeuAsp: 6.119 ± 0.069	4.607LeuGlu: 4.607 ± 0.065	3.254LeuPhe: 3.254 ± 0.049	7.847LeuGly: 7.847 ± 0.084	2.332LeuHis: 2.332 ± 0.048	4.283LeuIle: 4.283 ± 0.054	3.446LeuLys: 3.446 ± 0.054	10.879LeuLeu: 10.879 ± 0.133	2.332LeuMet: 2.332 ± 0.042	2.923LeuAsn: 2.923 ± 0.051	5.866LeuPro: 5.866 ± 0.072	4.175LeuGln: 4.175 ± 0.064	7.16LeuArg: 7.16 ± 0.083	5.92LeuSer: 5.92 ± 0.066	5.967LeuThr: 5.967 ± 0.069	7.515LeuVal: 7.515 ± 0.074	1.157LeuTrp: 1.157 ± 0.036	2.285LeuTyr: 2.285 ± 0.041	0.002LeuXaa: 0.002 ± 0.001
Met	3.018MetAla: 3.018 ± 0.051	0.138MetCys: 0.138 ± 0.009	1.152MetAsp: 1.152 ± 0.029	1.061MetGlu: 1.061 ± 0.028	0.734MetPhe: 0.734 ± 0.018	1.648MetGly: 1.648 ± 0.035	0.553MetHis: 0.553 ± 0.019	1.056MetIle: 1.056 ± 0.026	0.961MetLys: 0.961 ± 0.026	2.875MetLeu: 2.875 ± 0.047	0.62MetMet: 0.62 ± 0.023	0.917MetAsn: 0.917 ± 0.024	1.41MetPro: 1.41 ± 0.034	1.118MetGln: 1.118 ± 0.028	1.645MetArg: 1.645 ± 0.037	1.477MetSer: 1.477 ± 0.028	1.648MetThr: 1.648 ± 0.032	1.837MetVal: 1.837 ± 0.037	0.188MetTrp: 0.188 ± 0.012	0.457MetTyr: 0.457 ± 0.018	0.001MetXaa: 0.001 ± 0.001
Asn	3.688AsnAla: 3.688 ± 0.055	0.239AsnCys: 0.239 ± 0.014	1.612AsnAsp: 1.612 ± 0.033	1.211AsnGlu: 1.211 ± 0.03	1.093AsnPhe: 1.093 ± 0.029	2.806AsnGly: 2.806 ± 0.069	0.61AsnHis: 0.61 ± 0.02	1.483AsnIle: 1.483 ± 0.034	1.005AsnLys: 1.005 ± 0.027	3.01AsnLeu: 3.01 ± 0.041	0.643AsnMet: 0.643 ± 0.019	1.12AsnAsn: 1.12 ± 0.037	1.846AsnPro: 1.846 ± 0.041	1.146AsnGln: 1.146 ± 0.033	1.774AsnArg: 1.774 ± 0.033	1.457AsnSer: 1.457 ± 0.035	1.761AsnThr: 1.761 ± 0.042	2.174AsnVal: 2.174 ± 0.047	0.443AsnTrp: 0.443 ± 0.017	0.902AsnTyr: 0.902 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	7.821ProAla: 7.821 ± 0.109	0.324ProCys: 0.324 ± 0.016	3.239ProAsp: 3.239 ± 0.049	2.721ProGlu: 2.721 ± 0.047	1.664ProPhe: 1.664 ± 0.029	4.332ProGly: 4.332 ± 0.064	1.085ProHis: 1.085 ± 0.031	1.709ProIle: 1.709 ± 0.033	1.341ProLys: 1.341 ± 0.035	5.043ProLeu: 5.043 ± 0.064	1.141ProMet: 1.141 ± 0.03	1.273ProAsn: 1.273 ± 0.027	2.539ProPro: 2.539 ± 0.051	2.161ProGln: 2.161 ± 0.042	2.628ProArg: 2.628 ± 0.047	2.488ProSer: 2.488 ± 0.041	2.394ProThr: 2.394 ± 0.045	4.321ProVal: 4.321 ± 0.062	0.608ProTrp: 0.608 ± 0.022	1.175ProTyr: 1.175 ± 0.03	0.001ProXaa: 0.001 ± 0.001
Gln	5.862GlnAla: 5.862 ± 0.086	0.271GlnCys: 0.271 ± 0.013	1.795GlnAsp: 1.795 ± 0.04	1.613GlnGlu: 1.613 ± 0.034	1.433GlnPhe: 1.433 ± 0.032	2.888GlnGly: 2.888 ± 0.045	1.078GlnHis: 1.078 ± 0.025	1.889GlnIle: 1.889 ± 0.039	1.204GlnLys: 1.204 ± 0.034	4.803GlnLeu: 4.803 ± 0.067	1.029GlnMet: 1.029 ± 0.026	1.028GlnAsn: 1.028 ± 0.028	2.324GlnPro: 2.324 ± 0.041	2.623GlnGln: 2.623 ± 0.053	3.342GlnArg: 3.342 ± 0.063	2.008GlnSer: 2.008 ± 0.035	2.027GlnThr: 2.027 ± 0.038	3.251GlnVal: 3.251 ± 0.045	0.578GlnTrp: 0.578 ± 0.018	0.977GlnTyr: 0.977 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.847ArgAla: 7.847 ± 0.087	0.489ArgCys: 0.489 ± 0.018	3.795ArgAsp: 3.795 ± 0.067	3.426ArgGlu: 3.426 ± 0.062	2.4ArgPhe: 2.4 ± 0.04	4.034ArgGly: 4.034 ± 0.06	1.854ArgHis: 1.854 ± 0.04	3.308ArgIle: 3.308 ± 0.046	2.067ArgLys: 2.067 ± 0.042	7.153ArgLeu: 7.153 ± 0.093	1.732ArgMet: 1.732 ± 0.034	1.989ArgAsn: 1.989 ± 0.034	2.796ArgPro: 2.796 ± 0.049	3.295ArgGln: 3.295 ± 0.053	4.543ArgArg: 4.543 ± 0.065	3.305ArgSer: 3.305 ± 0.048	3.298ArgThr: 3.298 ± 0.048	4.44ArgVal: 4.44 ± 0.062	0.985ArgTrp: 0.985 ± 0.026	2.113ArgTyr: 2.113 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	7.09SerAla: 7.09 ± 0.082	0.417SerCys: 0.417 ± 0.017	2.949SerAsp: 2.949 ± 0.046	2.228SerGlu: 2.228 ± 0.044	2.026SerPhe: 2.026 ± 0.039	5.325SerGly: 5.325 ± 0.087	1.15SerHis: 1.15 ± 0.029	2.455SerIle: 2.455 ± 0.046	1.737SerLys: 1.737 ± 0.046	5.441SerLeu: 5.441 ± 0.064	1.343SerMet: 1.343 ± 0.031	1.728SerAsn: 1.728 ± 0.043	2.479SerPro: 2.479 ± 0.043	1.949SerGln: 1.949 ± 0.033	3.184SerArg: 3.184 ± 0.053	3.202SerSer: 3.202 ± 0.056	3.012SerThr: 3.012 ± 0.052	3.929SerVal: 3.929 ± 0.057	0.733SerTrp: 0.733 ± 0.026	1.501SerTyr: 1.501 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	7.166ThrAla: 7.166 ± 0.097	0.378ThrCys: 0.378 ± 0.015	2.899ThrAsp: 2.899 ± 0.043	2.37ThrGlu: 2.37 ± 0.044	1.788ThrPhe: 1.788 ± 0.038	5.158ThrGly: 5.158 ± 0.083	0.972ThrHis: 0.972 ± 0.029	2.672ThrIle: 2.672 ± 0.047	1.297ThrLys: 1.297 ± 0.04	6.306ThrLeu: 6.306 ± 0.088	1.265ThrMet: 1.265 ± 0.034	1.466ThrAsn: 1.466 ± 0.033	3.557ThrPro: 3.557 ± 0.051	1.754ThrGln: 1.754 ± 0.032	3.235ThrArg: 3.235 ± 0.046	2.971ThrSer: 2.971 ± 0.057	3.202ThrThr: 3.202 ± 0.059	4.909ThrVal: 4.909 ± 0.072	0.664ThrTrp: 0.664 ± 0.021	1.329ThrTyr: 1.329 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	9.869ValAla: 9.869 ± 0.104	0.658ValCys: 0.658 ± 0.022	4.019ValAsp: 4.019 ± 0.06	3.703ValGlu: 3.703 ± 0.05	2.48ValPhe: 2.48 ± 0.043	5.415ValGly: 5.415 ± 0.072	1.542ValHis: 1.542 ± 0.034	3.584ValIle: 3.584 ± 0.058	2.609ValLys: 2.609 ± 0.054	7.994ValLeu: 7.994 ± 0.078	1.869ValMet: 1.869 ± 0.033	2.362ValAsn: 2.362 ± 0.041	3.616ValPro: 3.616 ± 0.054	2.835ValGln: 2.835 ± 0.051	4.836ValArg: 4.836 ± 0.057	4.192ValSer: 4.192 ± 0.058	4.629ValThr: 4.629 ± 0.067	5.939ValVal: 5.939 ± 0.068	0.831ValTrp: 0.831 ± 0.029	1.666ValTyr: 1.666 ± 0.031	0.001ValXaa: 0.001 ± 0.001
Trp	1.07TrpAla: 1.07 ± 0.03	0.144TrpCys: 0.144 ± 0.01	0.629TrpAsp: 0.629 ± 0.02	0.494TrpGlu: 0.494 ± 0.016	0.503TrpPhe: 0.503 ± 0.018	0.77TrpGly: 0.77 ± 0.024	0.344TrpHis: 0.344 ± 0.017	0.62TrpIle: 0.62 ± 0.02	0.422TrpLys: 0.422 ± 0.016	1.755TrpLeu: 1.755 ± 0.045	0.369TrpMet: 0.369 ± 0.018	0.447TrpAsn: 0.447 ± 0.017	0.605TrpPro: 0.605 ± 0.021	0.794TrpGln: 0.794 ± 0.024	1.154TrpArg: 1.154 ± 0.033	0.76TrpSer: 0.76 ± 0.025	0.732TrpThr: 0.732 ± 0.022	0.786TrpVal: 0.786 ± 0.023	0.242TrpTrp: 0.242 ± 0.015	0.343TrpTyr: 0.343 ± 0.019	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.967TyrAla: 2.967 ± 0.048	0.261TyrCys: 0.261 ± 0.013	1.551TyrAsp: 1.551 ± 0.032	1.114TyrGlu: 1.114 ± 0.025	1.013TyrPhe: 1.013 ± 0.027	2.132TyrGly: 2.132 ± 0.042	0.529TyrHis: 0.529 ± 0.018	0.945TyrIle: 0.945 ± 0.028	0.801TyrLys: 0.801 ± 0.022	2.674TyrLeu: 2.674 ± 0.048	0.493TyrMet: 0.493 ± 0.019	0.839TyrAsn: 0.839 ± 0.027	1.229TyrPro: 1.229 ± 0.029	1.129TyrGln: 1.129 ± 0.028	1.903TyrArg: 1.903 ± 0.039	1.333TyrSer: 1.333 ± 0.033	1.525TyrThr: 1.525 ± 0.038	1.764TyrVal: 1.764 ± 0.033	0.359TyrTrp: 0.359 ± 0.017	0.74TyrTyr: 0.74 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4312 proteins (1538618 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---