Novosphingobium sp. FSW06-99

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4210 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A124JZ66|A0A124JZ66_9SPHN Transporter OS=Novosphingobium sp. FSW06-99 OX=1739113 GN=AQZ49_05765 PE=3 SV=1
MM1 pKa = 7.57PADD4 pKa = 3.93EE5 pKa = 5.55PLDD8 pKa = 3.88CLHH11 pKa = 6.8IFSHH15 pKa = 6.16AQALFPGATIDD26 pKa = 4.52VIHH29 pKa = 6.68TPDD32 pKa = 4.12EE33 pKa = 4.74IIHH36 pKa = 6.66LDD38 pKa = 3.93VNGHH42 pKa = 6.67RR43 pKa = 11.84YY44 pKa = 7.01TFEE47 pKa = 4.31SGSDD51 pKa = 3.18DD52 pKa = 3.87DD53 pKa = 5.69EE54 pKa = 5.07YY55 pKa = 11.77VFSDD59 pKa = 3.93GEE61 pKa = 4.54TTFTIPLMDD70 pKa = 4.35FDD72 pKa = 5.29EE73 pKa = 5.18DD74 pKa = 3.92FF75 pKa = 4.57

Molecular weight: 8.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A102DFR6|A0A102DFR6_9SPHN HTH lysR-type domain-containing protein OS=Novosphingobium sp. FSW06-99 OX=1739113 GN=AQZ49_00310 PE=3 SV=1
MM1 pKa = 6.36TTSDD5 pKa = 3.13RR6 pKa = 11.84RR7 pKa = 11.84TARR10 pKa = 11.84GPMRR14 pKa = 11.84AIVDD18 pKa = 3.32IGSNTVRR25 pKa = 11.84LVIYY29 pKa = 9.01GGPLRR34 pKa = 11.84APAVLHH40 pKa = 5.65NEE42 pKa = 3.87KK43 pKa = 9.17VTARR47 pKa = 11.84LGKK50 pKa = 10.2GVAEE54 pKa = 4.27NGLLGTRR61 pKa = 11.84ATTSVLAALARR72 pKa = 11.84YY73 pKa = 8.58RR74 pKa = 11.84VLLQLKK80 pKa = 8.63GVEE83 pKa = 4.19RR84 pKa = 11.84VDD86 pKa = 3.92VVATAAVRR94 pKa = 11.84DD95 pKa = 4.53AINGGAFLEE104 pKa = 4.51RR105 pKa = 11.84VAAMGFSPRR114 pKa = 11.84LLSGEE119 pKa = 4.11QEE121 pKa = 4.51AVTSAHH127 pKa = 5.98GVLGAFPGASGVVGDD142 pKa = 4.85LGGGSLEE149 pKa = 4.77LVDD152 pKa = 5.72IDD154 pKa = 5.54GDD156 pKa = 4.03TCRR159 pKa = 11.84HH160 pKa = 5.29GVSMPLGTLRR170 pKa = 11.84LGRR173 pKa = 11.84LRR175 pKa = 11.84AAGDD179 pKa = 3.36RR180 pKa = 11.84AFGQQVAKK188 pKa = 10.43ALKK191 pKa = 9.13KK192 pKa = 10.51ADD194 pKa = 3.26WAAEE198 pKa = 4.08PGSTLYY204 pKa = 11.05LVGGSLRR211 pKa = 11.84AFARR215 pKa = 11.84FAMARR220 pKa = 11.84LEE222 pKa = 4.06WPIDD226 pKa = 3.81DD227 pKa = 3.59PHH229 pKa = 8.69GFVLDD234 pKa = 3.95PADD237 pKa = 3.72GLRR240 pKa = 11.84IARR243 pKa = 11.84ALARR247 pKa = 11.84RR248 pKa = 11.84KK249 pKa = 10.1PEE251 pKa = 3.83TLRR254 pKa = 11.84PLPGISTARR263 pKa = 11.84MNSLPDD269 pKa = 3.44TAALLAVLIRR279 pKa = 11.84RR280 pKa = 11.84LQPGKK285 pKa = 10.49LVFSAWGLRR294 pKa = 11.84EE295 pKa = 3.83GLLYY299 pKa = 10.89EE300 pKa = 5.11DD301 pKa = 4.34MPAGIRR307 pKa = 11.84GQDD310 pKa = 3.58PLVAGMSAFVQEE322 pKa = 4.71HH323 pKa = 7.28GIPAQIGAMVAGWTVAAAPPSGVEE347 pKa = 3.3RR348 pKa = 11.84RR349 pKa = 11.84EE350 pKa = 3.94RR351 pKa = 11.84LRR353 pKa = 11.84LAATMLCLASAVVEE367 pKa = 4.18PNLRR371 pKa = 11.84GDD373 pKa = 3.96IARR376 pKa = 11.84DD377 pKa = 2.8WALRR381 pKa = 11.84KK382 pKa = 9.33RR383 pKa = 11.84WIGATTTEE391 pKa = 3.79RR392 pKa = 11.84VMLAVAMLGHH402 pKa = 7.65AGRR405 pKa = 11.84LDD407 pKa = 3.72VPPALAALITPAALRR422 pKa = 11.84EE423 pKa = 4.11AQTWGLATRR432 pKa = 11.84LCRR435 pKa = 11.84RR436 pKa = 11.84FSTCAPQSLTDD447 pKa = 3.48SQLTVEE453 pKa = 4.71GTRR456 pKa = 11.84LVLSVQPALAALVNEE471 pKa = 4.32GVEE474 pKa = 3.95RR475 pKa = 11.84DD476 pKa = 3.67LRR478 pKa = 11.84ALATHH483 pKa = 7.18LGLKK487 pKa = 10.5AEE489 pKa = 4.33VRR491 pKa = 3.88

Molecular weight: 51.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4210

0

4210

1418537

29

4323

336.9

36.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.185 ± 0.062

0.854 ± 0.012

6.011 ± 0.03

4.372 ± 0.035

3.497 ± 0.021

9.032 ± 0.049

2.402 ± 0.02

4.899 ± 0.024

2.26 ± 0.022

10.042 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.277 ± 0.018

2.513 ± 0.039

5.549 ± 0.029

3.225 ± 0.023

7.125 ± 0.046

4.834 ± 0.038

5.664 ± 0.056

7.482 ± 0.03

1.519 ± 0.019

2.258 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski