Amino acid dipepetide frequency for Novosphingobium sp. FSW06-99

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.396AlaAla: 20.396 ± 0.182	1.208AlaCys: 1.208 ± 0.034	8.354AlaAsp: 8.354 ± 0.088	6.438AlaGlu: 6.438 ± 0.078	4.455AlaPhe: 4.455 ± 0.06	12.428AlaGly: 12.428 ± 0.123	3.181AlaHis: 3.181 ± 0.055	7.159AlaIle: 7.159 ± 0.076	2.999AlaLys: 2.999 ± 0.054	15.955AlaLeu: 15.955 ± 0.155	4.09AlaMet: 4.09 ± 0.052	3.323AlaAsn: 3.323 ± 0.061	7.185AlaPro: 7.185 ± 0.099	5.42AlaGln: 5.42 ± 0.071	11.147AlaArg: 11.147 ± 0.123	6.514AlaSer: 6.514 ± 0.085	7.458AlaThr: 7.458 ± 0.072	9.396AlaVal: 9.396 ± 0.091	1.97AlaTrp: 1.97 ± 0.039	2.774AlaTyr: 2.774 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.224CysAla: 1.224 ± 0.032	0.103CysCys: 0.103 ± 0.01	0.559CysAsp: 0.559 ± 0.023	0.36CysGlu: 0.36 ± 0.015	0.297CysPhe: 0.297 ± 0.016	0.935CysGly: 0.935 ± 0.025	0.262CysHis: 0.262 ± 0.016	0.354CysIle: 0.354 ± 0.017	0.133CysLys: 0.133 ± 0.01	0.836CysLeu: 0.836 ± 0.026	0.161CysMet: 0.161 ± 0.01	0.213CysAsn: 0.213 ± 0.011	0.51CysPro: 0.51 ± 0.019	0.225CysGln: 0.225 ± 0.011	0.573CysArg: 0.573 ± 0.02	0.424CysSer: 0.424 ± 0.019	0.46CysThr: 0.46 ± 0.021	0.572CysVal: 0.572 ± 0.021	0.133CysTrp: 0.133 ± 0.01	0.208CysTyr: 0.208 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.085AspAla: 8.085 ± 0.088	0.523AspCys: 0.523 ± 0.022	3.442AspAsp: 3.442 ± 0.057	2.802AspGlu: 2.802 ± 0.051	2.058AspPhe: 2.058 ± 0.042	5.408AspGly: 5.408 ± 0.067	1.769AspHis: 1.769 ± 0.036	2.724AspIle: 2.724 ± 0.046	1.425AspLys: 1.425 ± 0.034	6.247AspLeu: 6.247 ± 0.073	1.256AspMet: 1.256 ± 0.027	1.388AspAsn: 1.388 ± 0.037	4.043AspPro: 4.043 ± 0.056	2.048AspGln: 2.048 ± 0.037	4.787AspArg: 4.787 ± 0.063	2.271AspSer: 2.271 ± 0.04	3.019AspThr: 3.019 ± 0.052	4.077AspVal: 4.077 ± 0.06	1.179AspTrp: 1.179 ± 0.03	1.557AspTyr: 1.557 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	6.799GluAla: 6.799 ± 0.085	0.308GluCys: 0.308 ± 0.016	2.191GluAsp: 2.191 ± 0.041	1.791GluGlu: 1.791 ± 0.044	1.459GluPhe: 1.459 ± 0.032	3.428GluGly: 3.428 ± 0.046	1.094GluHis: 1.094 ± 0.032	2.325GluIle: 2.325 ± 0.043	1.16GluLys: 1.16 ± 0.034	4.101GluLeu: 4.101 ± 0.06	1.017GluMet: 1.017 ± 0.03	1.074GluAsn: 1.074 ± 0.027	2.306GluPro: 2.306 ± 0.048	1.688GluGln: 1.688 ± 0.037	3.893GluArg: 3.893 ± 0.057	1.724GluSer: 1.724 ± 0.036	2.775GluThr: 2.775 ± 0.039	3.126GluVal: 3.126 ± 0.057	0.665GluTrp: 0.665 ± 0.021	0.8GluTyr: 0.8 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	5.341PheAla: 5.341 ± 0.063	0.358PheCys: 0.358 ± 0.016	2.787PheAsp: 2.787 ± 0.045	1.659PheGlu: 1.659 ± 0.035	1.148PhePhe: 1.148 ± 0.033	3.762PheGly: 3.762 ± 0.065	0.823PheHis: 0.823 ± 0.03	1.349PheIle: 1.349 ± 0.037	0.721PheLys: 0.721 ± 0.023	2.698PheLeu: 2.698 ± 0.057	0.69PheMet: 0.69 ± 0.02	1.136PheAsn: 1.136 ± 0.036	1.486PhePro: 1.486 ± 0.033	0.855PheGln: 0.855 ± 0.03	2.047PheArg: 2.047 ± 0.044	1.905PheSer: 1.905 ± 0.042	2.021PheThr: 2.021 ± 0.034	2.74PheVal: 2.74 ± 0.05	0.489PheTrp: 0.489 ± 0.018	0.957PheTyr: 0.957 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	11.012GlyAla: 11.012 ± 0.096	0.868GlyCys: 0.868 ± 0.025	4.926GlyAsp: 4.926 ± 0.065	4.122GlyGlu: 4.122 ± 0.057	3.659GlyPhe: 3.659 ± 0.056	8.176GlyGly: 8.176 ± 0.134	2.379GlyHis: 2.379 ± 0.05	4.279GlyIle: 4.279 ± 0.056	2.68GlyLys: 2.68 ± 0.046	9.308GlyLeu: 9.308 ± 0.085	2.216GlyMet: 2.216 ± 0.04	2.32GlyAsn: 2.32 ± 0.063	4.023GlyPro: 4.023 ± 0.055	3.31GlyGln: 3.31 ± 0.05	5.86GlyArg: 5.86 ± 0.071	4.694GlySer: 4.694 ± 0.1	5.572GlyThr: 5.572 ± 0.124	6.625GlyVal: 6.625 ± 0.067	1.762GlyTrp: 1.762 ± 0.042	2.531GlyTyr: 2.531 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	3.271HisAla: 3.271 ± 0.059	0.271HisCys: 0.271 ± 0.014	1.681HisAsp: 1.681 ± 0.041	0.986HisGlu: 0.986 ± 0.026	0.964HisPhe: 0.964 ± 0.028	2.486HisGly: 2.486 ± 0.047	0.737HisHis: 0.737 ± 0.027	0.947HisIle: 0.947 ± 0.03	0.45HisLys: 0.45 ± 0.018	2.285HisLeu: 2.285 ± 0.04	0.48HisMet: 0.48 ± 0.019	0.501HisAsn: 0.501 ± 0.02	1.589HisPro: 1.589 ± 0.033	0.669HisGln: 0.669 ± 0.024	1.712HisArg: 1.712 ± 0.038	0.993HisSer: 0.993 ± 0.025	1.02HisThr: 1.02 ± 0.025	1.883HisVal: 1.883 ± 0.039	0.426HisTrp: 0.426 ± 0.016	0.672HisTyr: 0.672 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	8.524IleAla: 8.524 ± 0.085	0.462IleCys: 0.462 ± 0.021	4.198IleAsp: 4.198 ± 0.051	3.083IleGlu: 3.083 ± 0.054	1.207IlePhe: 1.207 ± 0.032	5.195IleGly: 5.195 ± 0.057	0.947IleHis: 0.947 ± 0.027	1.756IleIle: 1.756 ± 0.042	1.145IleLys: 1.145 ± 0.03	3.303IleLeu: 3.303 ± 0.052	0.829IleMet: 0.829 ± 0.027	1.494IleAsn: 1.494 ± 0.039	2.125IlePro: 2.125 ± 0.04	1.031IleGln: 1.031 ± 0.035	2.917IleArg: 2.917 ± 0.05	2.415IleSer: 2.415 ± 0.053	2.846IleThr: 2.846 ± 0.056	3.853IleVal: 3.853 ± 0.054	0.581IleTrp: 0.581 ± 0.023	1.08IleTyr: 1.08 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.178LysAla: 3.178 ± 0.058	0.117LysCys: 0.117 ± 0.009	1.137LysAsp: 1.137 ± 0.037	0.745LysGlu: 0.745 ± 0.029	0.706LysPhe: 0.706 ± 0.02	2.058LysGly: 2.058 ± 0.047	0.446LysHis: 0.446 ± 0.018	1.093LysIle: 1.093 ± 0.03	0.641LysLys: 0.641 ± 0.026	2.527LysLeu: 2.527 ± 0.054	0.513LysMet: 0.513 ± 0.022	0.509LysAsn: 0.509 ± 0.02	1.581LysPro: 1.581 ± 0.044	0.67LysGln: 0.67 ± 0.024	1.518LysArg: 1.518 ± 0.035	1.179LysSer: 1.179 ± 0.032	1.328LysThr: 1.328 ± 0.03	1.846LysVal: 1.846 ± 0.045	0.307LysTrp: 0.307 ± 0.016	0.504LysTyr: 0.504 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	16.192LeuAla: 16.192 ± 0.143	0.9LeuCys: 0.9 ± 0.029	6.283LeuAsp: 6.283 ± 0.067	3.818LeuGlu: 3.818 ± 0.06	3.569LeuPhe: 3.569 ± 0.059	8.997LeuGly: 8.997 ± 0.089	2.154LeuHis: 2.154 ± 0.039	5.069LeuIle: 5.069 ± 0.061	2.244LeuLys: 2.244 ± 0.045	8.861LeuLeu: 8.861 ± 0.099	2.003LeuMet: 2.003 ± 0.044	2.326LeuAsn: 2.326 ± 0.048	5.874LeuPro: 5.874 ± 0.073	2.553LeuGln: 2.553 ± 0.047	6.968LeuArg: 6.968 ± 0.091	5.475LeuSer: 5.475 ± 0.06	5.939LeuThr: 5.939 ± 0.083	7.976LeuVal: 7.976 ± 0.08	1.291LeuTrp: 1.291 ± 0.039	1.934LeuTyr: 1.934 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	3.471MetAla: 3.471 ± 0.06	0.161MetCys: 0.161 ± 0.012	0.974MetAsp: 0.974 ± 0.026	0.778MetGlu: 0.778 ± 0.023	0.62MetPhe: 0.62 ± 0.021	1.718MetGly: 1.718 ± 0.038	0.477MetHis: 0.477 ± 0.017	1.384MetIle: 1.384 ± 0.028	0.658MetLys: 0.658 ± 0.022	2.469MetLeu: 2.469 ± 0.044	0.544MetMet: 0.544 ± 0.02	0.582MetAsn: 0.582 ± 0.019	1.418MetPro: 1.418 ± 0.029	0.687MetGln: 0.687 ± 0.02	1.662MetArg: 1.662 ± 0.036	1.124MetSer: 1.124 ± 0.028	1.839MetThr: 1.839 ± 0.032	1.772MetVal: 1.772 ± 0.047	0.195MetTrp: 0.195 ± 0.012	0.234MetTyr: 0.234 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.418AsnAla: 3.418 ± 0.062	0.233AsnCys: 0.233 ± 0.014	1.303AsnAsp: 1.303 ± 0.036	0.871AsnGlu: 0.871 ± 0.027	0.95AsnPhe: 0.95 ± 0.029	2.406AsnGly: 2.406 ± 0.072	0.542AsnHis: 0.542 ± 0.02	1.14AsnIle: 1.14 ± 0.035	0.465AsnLys: 0.465 ± 0.017	2.531AsnLeu: 2.531 ± 0.049	0.467AsnMet: 0.467 ± 0.018	0.72AsnAsn: 0.72 ± 0.035	1.858AsnPro: 1.858 ± 0.04	0.801AsnGln: 0.801 ± 0.031	1.765AsnArg: 1.765 ± 0.036	1.248AsnSer: 1.248 ± 0.052	1.403AsnThr: 1.403 ± 0.053	1.839AsnVal: 1.839 ± 0.039	0.417AsnTrp: 0.417 ± 0.019	0.758AsnTyr: 0.758 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	8.024ProAla: 8.024 ± 0.099	0.383ProCys: 0.383 ± 0.017	4.289ProAsp: 4.289 ± 0.063	2.832ProGlu: 2.832 ± 0.054	1.903ProPhe: 1.903 ± 0.038	5.523ProGly: 5.523 ± 0.068	1.336ProHis: 1.336 ± 0.033	2.431ProIle: 2.431 ± 0.048	1.091ProLys: 1.091 ± 0.032	5.339ProLeu: 5.339 ± 0.073	1.22ProMet: 1.22 ± 0.029	1.206ProAsn: 1.206 ± 0.03	3.0ProPro: 3.0 ± 0.073	1.776ProGln: 1.776 ± 0.035	3.309ProArg: 3.309 ± 0.05	2.315ProSer: 2.315 ± 0.046	2.794ProThr: 2.794 ± 0.051	4.817ProVal: 4.817 ± 0.067	0.817ProTrp: 0.817 ± 0.026	1.083ProTyr: 1.083 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.789GlnAla: 4.789 ± 0.064	0.254GlnCys: 0.254 ± 0.014	1.439GlnAsp: 1.439 ± 0.035	0.958GlnGlu: 0.958 ± 0.025	1.226GlnPhe: 1.226 ± 0.03	2.732GlnGly: 2.732 ± 0.047	0.716GlnHis: 0.716 ± 0.019	1.974GlnIle: 1.974 ± 0.039	0.685GlnLys: 0.685 ± 0.022	2.821GlnLeu: 2.821 ± 0.052	0.804GlnMet: 0.804 ± 0.027	0.756GlnAsn: 0.756 ± 0.03	1.963GlnPro: 1.963 ± 0.042	1.148GlnGln: 1.148 ± 0.032	2.425GlnArg: 2.425 ± 0.043	1.796GlnSer: 1.796 ± 0.039	2.017GlnThr: 2.017 ± 0.039	2.512GlnVal: 2.512 ± 0.039	0.551GlnTrp: 0.551 ± 0.02	0.683GlnTyr: 0.683 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.825ArgAla: 9.825 ± 0.122	0.49ArgCys: 0.49 ± 0.018	4.297ArgAsp: 4.297 ± 0.072	3.351ArgGlu: 3.351 ± 0.055	2.996ArgPhe: 2.996 ± 0.046	4.897ArgGly: 4.897 ± 0.076	2.034ArgHis: 2.034 ± 0.042	3.904ArgIle: 3.904 ± 0.056	1.714ArgLys: 1.714 ± 0.043	8.251ArgLeu: 8.251 ± 0.08	1.738ArgMet: 1.738 ± 0.033	1.691ArgAsn: 1.691 ± 0.032	3.669ArgPro: 3.669 ± 0.061	2.57ArgGln: 2.57 ± 0.046	5.502ArgArg: 5.502 ± 0.081	3.16ArgSer: 3.16 ± 0.045	3.397ArgThr: 3.397 ± 0.051	4.864ArgVal: 4.864 ± 0.067	1.183ArgTrp: 1.183 ± 0.031	1.712ArgTyr: 1.712 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	6.494SerAla: 6.494 ± 0.074	0.355SerCys: 0.355 ± 0.017	2.735SerAsp: 2.735 ± 0.043	1.952SerGlu: 1.952 ± 0.04	1.822SerPhe: 1.822 ± 0.037	5.325SerGly: 5.325 ± 0.096	1.156SerHis: 1.156 ± 0.031	2.326SerIle: 2.326 ± 0.047	0.995SerLys: 0.995 ± 0.029	4.847SerLeu: 4.847 ± 0.072	1.014SerMet: 1.014 ± 0.031	1.271SerAsn: 1.271 ± 0.039	2.84SerPro: 2.84 ± 0.044	1.513SerGln: 1.513 ± 0.038	3.006SerArg: 3.006 ± 0.056	2.488SerSer: 2.488 ± 0.072	2.647SerThr: 2.647 ± 0.078	3.592SerVal: 3.592 ± 0.063	0.676SerTrp: 0.676 ± 0.02	1.283SerTyr: 1.283 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	6.987ThrAla: 6.987 ± 0.08	0.486ThrCys: 0.486 ± 0.021	2.971ThrAsp: 2.971 ± 0.045	2.041ThrGlu: 2.041 ± 0.041	1.92ThrPhe: 1.92 ± 0.041	5.876ThrGly: 5.876 ± 0.107	1.222ThrHis: 1.222 ± 0.023	3.121ThrIle: 3.121 ± 0.063	0.987ThrLys: 0.987 ± 0.028	6.262ThrLeu: 6.262 ± 0.078	1.288ThrMet: 1.288 ± 0.029	1.509ThrAsn: 1.509 ± 0.069	3.654ThrPro: 3.654 ± 0.054	1.654ThrGln: 1.654 ± 0.046	3.767ThrArg: 3.767 ± 0.049	2.742ThrSer: 2.742 ± 0.06	3.387ThrThr: 3.387 ± 0.101	4.648ThrVal: 4.648 ± 0.069	0.786ThrTrp: 0.786 ± 0.024	1.33ThrTyr: 1.33 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	10.415ValAla: 10.415 ± 0.094	0.644ValCys: 0.644 ± 0.022	4.18ValAsp: 4.18 ± 0.05	3.692ValGlu: 3.692 ± 0.053	2.541ValPhe: 2.541 ± 0.04	5.748ValGly: 5.748 ± 0.068	1.611ValHis: 1.611 ± 0.033	4.073ValIle: 4.073 ± 0.061	1.565ValLys: 1.565 ± 0.043	7.71ValLeu: 7.71 ± 0.088	1.75ValMet: 1.75 ± 0.038	1.925ValAsn: 1.925 ± 0.052	4.459ValPro: 4.459 ± 0.062	2.08ValGln: 2.08 ± 0.039	5.188ValArg: 5.188 ± 0.065	3.842ValSer: 3.842 ± 0.057	4.634ValThr: 4.634 ± 0.07	6.161ValVal: 6.161 ± 0.078	1.1ValTrp: 1.1 ± 0.03	1.501ValTyr: 1.501 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.456TrpAla: 1.456 ± 0.038	0.159TrpCys: 0.159 ± 0.009	0.789TrpAsp: 0.789 ± 0.023	0.477TrpGlu: 0.477 ± 0.017	0.633TrpPhe: 0.633 ± 0.023	1.017TrpGly: 1.017 ± 0.029	0.532TrpHis: 0.532 ± 0.021	0.738TrpIle: 0.738 ± 0.023	0.348TrpLys: 0.348 ± 0.016	1.974TrpLeu: 1.974 ± 0.043	0.323TrpMet: 0.323 ± 0.016	0.473TrpAsn: 0.473 ± 0.02	0.803TrpPro: 0.803 ± 0.026	0.754TrpGln: 0.754 ± 0.024	1.401TrpArg: 1.401 ± 0.038	0.926TrpSer: 0.926 ± 0.028	0.867TrpThr: 0.867 ± 0.028	0.895TrpVal: 0.895 ± 0.03	0.294TrpTrp: 0.294 ± 0.018	0.329TrpTyr: 0.329 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.951TyrAla: 2.951 ± 0.047	0.259TyrCys: 0.259 ± 0.013	1.572TyrAsp: 1.572 ± 0.035	0.969TyrGlu: 0.969 ± 0.028	0.842TyrPhe: 0.842 ± 0.027	2.209TyrGly: 2.209 ± 0.046	0.603TyrHis: 0.603 ± 0.026	0.846TyrIle: 0.846 ± 0.024	0.498TyrLys: 0.498 ± 0.02	2.099TyrLeu: 2.099 ± 0.039	0.364TyrMet: 0.364 ± 0.018	0.716TyrAsn: 0.716 ± 0.028	1.101TyrPro: 1.101 ± 0.027	0.797TyrGln: 0.797 ± 0.028	1.798TyrArg: 1.798 ± 0.036	1.102TyrSer: 1.102 ± 0.028	1.215TyrThr: 1.215 ± 0.034	1.622TyrVal: 1.622 ± 0.035	0.366TyrTrp: 0.366 ± 0.019	0.647TyrTyr: 0.647 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4210 proteins (1418537 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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