Candidatus Nitrospira nitrosa

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Nitrospira

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S4LLI8|A0A0S4LLI8_9BACT Lipoprotein-releasing system transmembrane protein LolC OS=Candidatus Nitrospira nitrosa OX=1742972 GN=lolC PE=4 SV=1
MM1 pKa = 7.06YY2 pKa = 8.14PARR5 pKa = 11.84SVSLLAFHH13 pKa = 6.92VNVADD18 pKa = 4.19EE19 pKa = 4.6LDD21 pKa = 3.88GALSVSVIVTVLMMLPLATVIVAWFSPCVAFEE53 pKa = 4.1VSTLAVMVPLADD65 pKa = 3.81PEE67 pKa = 4.37VV68 pKa = 3.35

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S4L6Q0|A0A0S4L6Q0_9BACT Prepilin leader peptidase/N-methyltransferase OS=Candidatus Nitrospira nitrosa OX=1742972 GN=pilD PE=3 SV=1
MM1 pKa = 7.61SFTFQQPSNLKK12 pKa = 9.85KK13 pKa = 10.33KK14 pKa = 9.99RR15 pKa = 11.84EE16 pKa = 3.76HH17 pKa = 6.33GFRR20 pKa = 11.84KK21 pKa = 10.0RR22 pKa = 11.84MSTKK26 pKa = 10.18NGRR29 pKa = 11.84KK30 pKa = 8.58VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.22GRR40 pKa = 11.84ARR42 pKa = 11.84LTVV45 pKa = 3.04

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4245

0

4245

1268829

21

3949

298.9

32.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.201 ± 0.038

1.04 ± 0.015

5.172 ± 0.037

5.943 ± 0.037

3.616 ± 0.022

7.833 ± 0.046

2.436 ± 0.022

5.349 ± 0.026

4.077 ± 0.037

10.521 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.019

2.828 ± 0.029

4.972 ± 0.025

4.187 ± 0.027

6.653 ± 0.037

6.121 ± 0.031

5.99 ± 0.041

7.688 ± 0.035

1.379 ± 0.019

2.596 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski