Daeseongdong virus 2
Average proteome isoelectric point is 7.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2RRD5|A0A0S2RRD5_9VIRU Putative capsid protein OS=Daeseongdong virus 2 OX=1758882 PE=4 SV=1
MM1 pKa = 7.54 DD2 pKa = 4.06 TSNPVMSSDD11 pKa = 2.98 RR12 pKa = 11.84 TTVEE16 pKa = 4.19 EE17 pKa = 3.87 ILEE20 pKa = 4.09 RR21 pKa = 11.84 SAGVKK26 pKa = 10.21 RR27 pKa = 11.84 QDD29 pKa = 3.23 LSFLGEE35 pKa = 3.9 VARR38 pKa = 11.84 TAKK41 pKa = 10.29 RR42 pKa = 11.84 EE43 pKa = 4.01 HH44 pKa = 6.72 LSCEE48 pKa = 3.86 IVFPPLEE55 pKa = 3.85 VRR57 pKa = 11.84 QIVSEE62 pKa = 4.22 FKK64 pKa = 10.56 KK65 pKa = 10.57 SIPRR69 pKa = 11.84 VEE71 pKa = 4.21 SEE73 pKa = 4.28 RR74 pKa = 11.84 SGEE77 pKa = 3.79 LDD79 pKa = 3.38 SEE81 pKa = 4.56 VFEE84 pKa = 4.44 VAMEE88 pKa = 4.0 KK89 pKa = 9.98 MPVCGLMDD97 pKa = 3.53 EE98 pKa = 4.47 QGIPLHH104 pKa = 6.31 PAGKK108 pKa = 8.6 NQTGGHH114 pKa = 4.71 VKK116 pKa = 10.11 VNRR119 pKa = 11.84 VFGKK123 pKa = 10.19 SAGVPLGLADD133 pKa = 3.24 IVEE136 pKa = 4.41 VFGPEE141 pKa = 3.62 VLQRR145 pKa = 11.84 KK146 pKa = 7.07 MVYY149 pKa = 9.68 TSGTWQGFKK158 pKa = 10.9 ARR160 pKa = 11.84 LKK162 pKa = 9.76 QQMSRR167 pKa = 11.84 KK168 pKa = 7.35 TVPLYY173 pKa = 9.99 KK174 pKa = 10.28 TMSEE178 pKa = 3.9 ARR180 pKa = 11.84 GAAKK184 pKa = 9.86 MGLNPALDD192 pKa = 4.56 QLDD195 pKa = 3.19 QWMPRR200 pKa = 11.84 GPNPNWPGVKK210 pKa = 9.17 TDD212 pKa = 3.79 IVDD215 pKa = 3.55 ALMGGIKK222 pKa = 8.7 VTANSSAGAPYY233 pKa = 9.03 WRR235 pKa = 11.84 NKK237 pKa = 8.61 GEE239 pKa = 4.25 CMEE242 pKa = 4.52 QILNVGLPIFVKK254 pKa = 10.33 AVKK257 pKa = 9.81 EE258 pKa = 4.03 NKK260 pKa = 10.08 LDD262 pKa = 4.05 DD263 pKa = 3.96 LWRR266 pKa = 11.84 KK267 pKa = 9.19 NPEE270 pKa = 3.76 FFLCEE275 pKa = 4.22 VKK277 pKa = 10.97 NKK279 pKa = 7.45 TDD281 pKa = 3.29 RR282 pKa = 11.84 YY283 pKa = 9.45 KK284 pKa = 11.05 VSEE287 pKa = 4.43 LTSKK291 pKa = 8.47 TRR293 pKa = 11.84 PYY295 pKa = 10.91 VSVPAHH301 pKa = 5.85 IAFLVSMLSQGFQEE315 pKa = 4.27 TLKK318 pKa = 10.98 VFTDD322 pKa = 4.44 DD323 pKa = 3.62 VTSSNAYY330 pKa = 9.91 GFSSTNGGLSKK341 pKa = 10.11 MVAWMRR347 pKa = 11.84 GADD350 pKa = 3.27 KK351 pKa = 10.68 RR352 pKa = 11.84 GRR354 pKa = 11.84 VCCYY358 pKa = 10.74 GDD360 pKa = 3.59 DD361 pKa = 3.67 ARR363 pKa = 11.84 IAVLRR368 pKa = 11.84 EE369 pKa = 3.65 GKK371 pKa = 9.89 VWVVDD376 pKa = 3.8 PDD378 pKa = 4.3 FKK380 pKa = 11.66 QMDD383 pKa = 3.79 GSIDD387 pKa = 3.57 GKK389 pKa = 10.59 DD390 pKa = 3.06 IEE392 pKa = 5.15 LTIEE396 pKa = 4.06 WILRR400 pKa = 11.84 HH401 pKa = 5.51 LRR403 pKa = 11.84 KK404 pKa = 10.08 DD405 pKa = 3.28 QGVSEE410 pKa = 5.09 TPAFWDD416 pKa = 5.34 GIAKK420 pKa = 8.87 VWKK423 pKa = 10.48 RR424 pKa = 11.84 MATDD428 pKa = 2.79 PHH430 pKa = 6.61 FVVDD434 pKa = 4.06 GTQVYY439 pKa = 9.87 RR440 pKa = 11.84 KK441 pKa = 9.4 RR442 pKa = 11.84 SRR444 pKa = 11.84 HH445 pKa = 4.57 GLMTGIPGTTLFDD458 pKa = 3.67 TVKK461 pKa = 10.8 SVGVWNTYY469 pKa = 10.73 LDD471 pKa = 3.87 LASINGWNPLDD482 pKa = 4.05 EE483 pKa = 5.79 DD484 pKa = 4.07 RR485 pKa = 11.84 ATDD488 pKa = 4.14 FMAKK492 pKa = 9.6 NGLVIKK498 pKa = 10.36 PGTWNPAVLPEE509 pKa = 5.68 DD510 pKa = 4.63 KK511 pKa = 10.63 DD512 pKa = 3.75 QQLLTDD518 pKa = 4.08 HH519 pKa = 7.09 KK520 pKa = 11.16 FLGTQIRR527 pKa = 11.84 CVDD530 pKa = 3.45 WEE532 pKa = 4.03 GHH534 pKa = 5.79 MIHH537 pKa = 6.53 VPTIPEE543 pKa = 3.8 SDD545 pKa = 3.73 ALEE548 pKa = 3.97 MLVTQKK554 pKa = 11.09 DD555 pKa = 3.74 DD556 pKa = 3.8 PFTKK560 pKa = 10.52 VSDD563 pKa = 3.77 TTQKK567 pKa = 8.69 RR568 pKa = 11.84 TLYY571 pKa = 10.19 EE572 pKa = 3.99 RR573 pKa = 11.84 MRR575 pKa = 11.84 GLYY578 pKa = 8.38 ITFGFEE584 pKa = 3.7 NDD586 pKa = 3.39 RR587 pKa = 11.84 VASAIHH593 pKa = 5.7 NVVNKK598 pKa = 10.19 LPAEE602 pKa = 4.63 IILMQTQIANGEE614 pKa = 4.17 RR615 pKa = 11.84 PEE617 pKa = 5.33 HH618 pKa = 6.2 ITLQDD623 pKa = 3.2 FNYY626 pKa = 8.93 PDD628 pKa = 3.44 SSGFPTRR635 pKa = 11.84 EE636 pKa = 3.61 FCYY639 pKa = 10.51 SLYY642 pKa = 10.92 SGLKK646 pKa = 7.91 SEE648 pKa = 4.47 RR649 pKa = 11.84 YY650 pKa = 8.97 GWIQIFPDD658 pKa = 4.34 LEE660 pKa = 4.2 GLLADD665 pKa = 4.79 LKK667 pKa = 10.71 SEE669 pKa = 3.87 RR670 pKa = 11.84 RR671 pKa = 11.84 QLGRR675 pKa = 11.84 KK676 pKa = 8.72 LRR678 pKa = 11.84 LALAEE683 pKa = 4.41 RR684 pKa = 11.84 SDD686 pKa = 3.49 KK687 pKa = 10.89 TRR689 pKa = 11.84 DD690 pKa = 3.34 VLYY693 pKa = 8.29 TTEE696 pKa = 4.74 GEE698 pKa = 4.34 SPPWDD703 pKa = 3.63 DD704 pKa = 3.43 VLEE707 pKa = 4.23 VAEE710 pKa = 4.17 ARR712 pKa = 11.84 EE713 pKa = 4.21 VPKK716 pKa = 10.29 MPVYY720 pKa = 9.47 STPNVRR726 pKa = 11.84 SEE728 pKa = 3.78 IARR731 pKa = 11.84 VVTSSNTIPAKK742 pKa = 10.22 RR743 pKa = 11.84 LPTRR747 pKa = 11.84 SEE749 pKa = 4.25 VVHH752 pKa = 6.22 NALTAVGGISTVGDD766 pKa = 4.01 LKK768 pKa = 10.77 RR769 pKa = 11.84 AAGVHH774 pKa = 4.81 TASLVEE780 pKa = 4.04 AAQSHH785 pKa = 6.58 GIFLAGTQDD794 pKa = 3.37 QDD796 pKa = 3.64 LCSLYY801 pKa = 10.25 PIATPTPTLQEE812 pKa = 4.01 SLIQNWQKK820 pKa = 11.63 SNDD823 pKa = 4.07 KK824 pKa = 10.38 IDD826 pKa = 3.91 GGSSHH831 pKa = 6.74 RR832 pKa = 11.84 RR833 pKa = 11.84 QALIALAQDD842 pKa = 3.28 RR843 pKa = 11.84 TIRR846 pKa = 11.84 TAPEE850 pKa = 3.61 KK851 pKa = 10.93 VYY853 pKa = 10.58 LANDD857 pKa = 3.47 VLVAIPDD864 pKa = 3.75 LLEE867 pKa = 4.55 PKK869 pKa = 10.41 DD870 pKa = 3.53 PDD872 pKa = 3.86 MIVAALNAPLTRR884 pKa = 11.84 LYY886 pKa = 11.17 GGMKK890 pKa = 9.62 WEE892 pKa = 4.09 VHH894 pKa = 5.81 PPQPKK899 pKa = 9.48 EE900 pKa = 3.56 INPIKK905 pKa = 8.96 VTLYY909 pKa = 9.99 VGAKK913 pKa = 9.07 EE914 pKa = 3.84 RR915 pKa = 11.84 LEE917 pKa = 4.13 RR918 pKa = 11.84 VAEE921 pKa = 4.04 ARR923 pKa = 11.84 SASIKK928 pKa = 9.9 LAKK931 pKa = 10.21 AYY933 pKa = 9.33 IARR936 pKa = 11.84 LIFEE940 pKa = 5.33 LNGFTLEE947 pKa = 4.13 EE948 pKa = 4.02 TRR950 pKa = 11.84 MTTTVKK956 pKa = 8.93 PTKK959 pKa = 9.55 NPKK962 pKa = 8.85 VISWADD968 pKa = 3.32 EE969 pKa = 4.21 VEE971 pKa = 4.21 EE972 pKa = 4.3 VEE974 pKa = 4.57 ALKK977 pKa = 10.91 GPYY980 pKa = 9.11 AAPPVEE986 pKa = 4.72 PIVFEE991 pKa = 4.04 QVPHH995 pKa = 6.74 LEE997 pKa = 3.9 AWKK1000 pKa = 10.22 IEE1002 pKa = 4.17 YY1003 pKa = 10.69 NLVPDD1008 pKa = 4.21 RR1009 pKa = 11.84 LFPILWRR1016 pKa = 11.84 YY1017 pKa = 9.31 AVTSEE1022 pKa = 4.3 EE1023 pKa = 3.83 PTEE1026 pKa = 4.12 AVFARR1031 pKa = 11.84 LLSKK1035 pKa = 10.56 KK1036 pKa = 9.02 QEE1038 pKa = 4.1 EE1039 pKa = 4.24 EE1040 pKa = 4.24 ANAQSGSGGPIYY1052 pKa = 10.08 RR1053 pKa = 11.84 DD1054 pKa = 3.29 PPEE1057 pKa = 4.27 DD1058 pKa = 3.3 QRR1060 pKa = 11.84 SRR1062 pKa = 11.84 RR1063 pKa = 11.84 SRR1065 pKa = 11.84 LSVNQRR1071 pKa = 11.84 QRR1073 pKa = 11.84 QNRR1076 pKa = 11.84 ALNRR1080 pKa = 11.84 RR1081 pKa = 11.84 RR1082 pKa = 11.84 RR1083 pKa = 11.84 GPPRR1087 pKa = 11.84 TDD1089 pKa = 2.89 PP1090 pKa = 3.84
Molecular weight: 122.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.734
IPC2_protein 7.102
IPC_protein 7.059
Toseland 7.0
ProMoST 7.658
Dawson 7.732
Bjellqvist 7.892
Wikipedia 7.658
Rodwell 7.746
Grimsley 7.117
Solomon 7.775
Lehninger 7.79
Nozaki 8.097
DTASelect 7.863
Thurlkill 7.922
EMBOSS 7.98
Sillero 8.156
Patrickios 4.724
IPC_peptide 7.775
IPC2_peptide 7.088
IPC2.peptide.svr19 7.243
Protein with the highest isoelectric point:
>tr|A0A0S2RRD5|A0A0S2RRD5_9VIRU Putative capsid protein OS=Daeseongdong virus 2 OX=1758882 PE=4 SV=1
MM1 pKa = 8.02 PSRR4 pKa = 11.84 KK5 pKa = 9.65 KK6 pKa = 10.42 NVNSCVCPHH15 pKa = 7.27 DD16 pKa = 4.49 GKK18 pKa = 10.83 QFSTRR23 pKa = 11.84 AALQQHH29 pKa = 6.32 KK30 pKa = 10.41 KK31 pKa = 8.74 MVHH34 pKa = 6.22 ASPNEE39 pKa = 3.99 TRR41 pKa = 11.84 PAPQPRR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 PRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 SGNGGGLSSDD63 pKa = 4.29 LAPSRR68 pKa = 11.84 VPTVRR73 pKa = 11.84 NSAITTSGSDD83 pKa = 2.98 KK84 pKa = 11.11 VLVFTIKK91 pKa = 10.46 AQQSLIKK98 pKa = 10.05 SIPIHH103 pKa = 6.5 PGSIPRR109 pKa = 11.84 LSNLAKK115 pKa = 10.08 SFQRR119 pKa = 11.84 ITWRR123 pKa = 11.84 SLAVHH128 pKa = 6.39 VSPQVSTSINGGYY141 pKa = 9.18 VAGIVTDD148 pKa = 4.45 PDD150 pKa = 4.11 DD151 pKa = 3.88 EE152 pKa = 6.36 HH153 pKa = 6.68 ITASDD158 pKa = 3.82 LSSTAGSQTKK168 pKa = 9.34 KK169 pKa = 9.08 WYY171 pKa = 8.97 QDD173 pKa = 3.41 AIIRR177 pKa = 11.84 MPLKK181 pKa = 9.7 TDD183 pKa = 3.13 KK184 pKa = 10.89 LYY186 pKa = 10.81 TSAGEE191 pKa = 4.13 EE192 pKa = 4.16 PRR194 pKa = 11.84 FSSPGTFWICSEE206 pKa = 4.49 GNPSSDD212 pKa = 2.93 LTVVVTFRR220 pKa = 11.84 WTVHH224 pKa = 6.2 LEE226 pKa = 3.95 GSTLEE231 pKa = 4.76 DD232 pKa = 3.96 DD233 pKa = 3.85 ATSSFVLSGSLNSIGGKK250 pKa = 10.08 SIMGYY255 pKa = 8.97 KK256 pKa = 10.15 ACGSTTVEE264 pKa = 4.11 EE265 pKa = 4.67 DD266 pKa = 3.39 VSGLIPAPIRR276 pKa = 11.84 EE277 pKa = 4.28 VPGTHH282 pKa = 6.8 FFRR285 pKa = 11.84 VPTYY289 pKa = 8.47 TVEE292 pKa = 4.08 YY293 pKa = 9.96 QEE295 pKa = 5.06 GVGDD299 pKa = 3.53 TGTIQMHH306 pKa = 5.01 FVAYY310 pKa = 8.57 KK311 pKa = 8.99 TDD313 pKa = 3.04 TKK315 pKa = 11.03 NFHH318 pKa = 6.45 FSEE321 pKa = 5.22 DD322 pKa = 3.6 GSSVNASDD330 pKa = 3.32 WQADD334 pKa = 3.02 VDD336 pKa = 4.1 NQVLCSDD343 pKa = 4.03 GTYY346 pKa = 10.54 FKK348 pKa = 10.88 YY349 pKa = 9.33 TGQGNACKK357 pKa = 10.09 VASTVAPRR365 pKa = 11.84 SSPSHH370 pKa = 6.64 LKK372 pKa = 9.24 RR373 pKa = 11.84 HH374 pKa = 5.11 SRR376 pKa = 11.84 EE377 pKa = 3.72 KK378 pKa = 11.07 LMSLSRR384 pKa = 11.84 KK385 pKa = 8.55 VASLEE390 pKa = 3.75 ASLKK394 pKa = 9.08 KK395 pKa = 10.59 FEE397 pKa = 4.76 MFSQRR402 pKa = 11.84 DD403 pKa = 3.69 SMTSSPEE410 pKa = 4.27 LIPSPEE416 pKa = 3.86 NLEE419 pKa = 4.15 LL420 pKa = 5.29
Molecular weight: 45.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.408
IPC2_protein 8.302
IPC_protein 8.2
Toseland 8.756
ProMoST 8.741
Dawson 9.136
Bjellqvist 9.092
Wikipedia 9.224
Rodwell 9.311
Grimsley 8.975
Solomon 9.238
Lehninger 9.209
Nozaki 9.297
DTASelect 8.946
Thurlkill 9.077
EMBOSS 9.297
Sillero 9.282
Patrickios 4.889
IPC_peptide 9.224
IPC2_peptide 7.819
IPC2.peptide.svr19 7.702
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1510
420
1090
755.0
84.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.887 ± 0.445
0.993 ± 0.207
5.629 ± 0.299
6.424 ± 0.904
3.179 ± 0.187
6.623 ± 0.134
1.987 ± 0.414
4.702 ± 0.085
6.093 ± 0.18
7.881 ± 0.804
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.318 ± 0.197
3.51 ± 0.084
6.49 ± 0.029
3.841 ± 0.015
6.623 ± 0.432
8.212 ± 2.663
6.689 ± 0.556
7.881 ± 0.351
1.722 ± 0.253
2.318 ± 0.083
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here