Staphylococcus phage IME1318_01
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6JNL4|A0A1W6JNL4_9CAUD Capsid and scaffold protein OS=Staphylococcus phage IME1318_01 OX=1965486 PE=4 SV=1
MM1 pKa = 7.16 RR2 pKa = 11.84 QSVNLQLFNYY12 pKa = 9.63 LYY14 pKa = 10.72 TKK16 pKa = 10.58 FEE18 pKa = 4.15 EE19 pKa = 4.53 LGVPIIRR26 pKa = 11.84 TSEE29 pKa = 3.87 LNQALPYY36 pKa = 9.96 PFIAIQSIRR45 pKa = 11.84 DD46 pKa = 3.96 DD47 pKa = 3.34 ISRR50 pKa = 11.84 LTFDD54 pKa = 4.47 SYY56 pKa = 11.69 GGSPTAIIHH65 pKa = 5.51 IWCTEE70 pKa = 3.88 DD71 pKa = 5.35 DD72 pKa = 3.63 KK73 pKa = 12.06 GKK75 pKa = 10.32 NDD77 pKa = 3.4 EE78 pKa = 5.54 LYY80 pKa = 10.43 IQVQSILLDD89 pKa = 4.41 EE90 pKa = 5.09 IEE92 pKa = 4.42 LDD94 pKa = 4.18 GYY96 pKa = 9.24 TLTLPQISVNEE107 pKa = 4.14 STEE110 pKa = 3.56 QDD112 pKa = 3.37 TNQTLSHH119 pKa = 5.09 TTISVEE125 pKa = 4.34 YY126 pKa = 10.0 ASHH129 pKa = 6.55
Molecular weight: 14.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.985
IPC2_protein 4.291
IPC_protein 4.202
Toseland 4.024
ProMoST 4.317
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.062
Rodwell 4.037
Grimsley 3.948
Solomon 4.151
Lehninger 4.113
Nozaki 4.279
DTASelect 4.444
Thurlkill 4.062
EMBOSS 4.075
Sillero 4.317
Patrickios 1.964
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.206
Protein with the highest isoelectric point:
>tr|A0A1W6JNK7|A0A1W6JNK7_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1318_01 OX=1965486 PE=4 SV=1
MM1 pKa = 7.4 YY2 pKa = 10.0 YY3 pKa = 10.68 SKK5 pKa = 11.42 GGTNEE10 pKa = 3.49 MNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 NKK18 pKa = 10.01 KK19 pKa = 8.78 EE20 pKa = 3.64 RR21 pKa = 11.84 QQEE24 pKa = 3.93 LTIWLMVASLIIQIIALIKK43 pKa = 9.91 TFFF46 pKa = 4.01
Molecular weight: 5.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.911
IPC_protein 10.672
Toseland 10.818
ProMoST 10.482
Dawson 10.921
Bjellqvist 10.599
Wikipedia 11.111
Rodwell 11.228
Grimsley 10.965
Solomon 11.023
Lehninger 10.994
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.818
EMBOSS 11.213
Sillero 10.833
Patrickios 11.023
IPC_peptide 11.038
IPC2_peptide 9.37
IPC2.peptide.svr19 8.438
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
13946
39
1257
193.7
22.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.736 ± 0.518
0.588 ± 0.096
6.669 ± 0.315
7.178 ± 0.47
3.786 ± 0.169
5.579 ± 0.182
1.836 ± 0.138
7.393 ± 0.302
8.712 ± 0.42
7.113 ± 0.233
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.61 ± 0.133
7.006 ± 0.309
2.725 ± 0.232
4.159 ± 0.271
4.474 ± 0.307
6.052 ± 0.317
6.339 ± 0.24
6.296 ± 0.375
1.262 ± 0.12
4.489 ± 0.374
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here