Amino acid dipepetide frequency for Staphylococcus phage IME1318_01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.721AlaAla: 1.721 ± 0.441
0.43AlaCys: 0.43 ± 0.168
4.589AlaAsp: 4.589 ± 0.699
4.303AlaGlu: 4.303 ± 0.622
3.227AlaPhe: 3.227 ± 0.717
3.227AlaGly: 3.227 ± 0.744
1.219AlaHis: 1.219 ± 0.242
4.876AlaIle: 4.876 ± 1.08
5.02AlaLys: 5.02 ± 0.632
3.801AlaLeu: 3.801 ± 0.722
1.864AlaMet: 1.864 ± 0.537
3.872AlaAsn: 3.872 ± 0.616
1.506AlaPro: 1.506 ± 0.356
2.582AlaGln: 2.582 ± 0.624
2.725AlaArg: 2.725 ± 0.397
3.155AlaSer: 3.155 ± 0.51
2.295AlaThr: 2.295 ± 0.641
3.944AlaVal: 3.944 ± 1.082
0.645AlaTrp: 0.645 ± 0.287
2.295AlaTyr: 2.295 ± 0.451
0.0AlaXaa: 0.0 ± 0.0
Cys
0.143CysAla: 0.143 ± 0.127
0.072CysCys: 0.072 ± 0.071
0.359CysAsp: 0.359 ± 0.159
0.359CysGlu: 0.359 ± 0.196
0.215CysPhe: 0.215 ± 0.132
0.502CysGly: 0.502 ± 0.165
0.287CysHis: 0.287 ± 0.155
0.502CysIle: 0.502 ± 0.199
0.359CysLys: 0.359 ± 0.175
0.287CysLeu: 0.287 ± 0.155
0.072CysMet: 0.072 ± 0.073
0.574CysAsn: 0.574 ± 0.226
0.215CysPro: 0.215 ± 0.097
0.072CysGln: 0.072 ± 0.077
0.502CysArg: 0.502 ± 0.221
0.359CysSer: 0.359 ± 0.162
0.359CysThr: 0.359 ± 0.153
0.359CysVal: 0.359 ± 0.154
0.0CysTrp: 0.0 ± 0.0
0.287CysTyr: 0.287 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
3.801AspAla: 3.801 ± 0.947
0.287AspCys: 0.287 ± 0.139
4.589AspAsp: 4.589 ± 0.658
5.02AspGlu: 5.02 ± 0.885
3.514AspPhe: 3.514 ± 0.566
3.944AspGly: 3.944 ± 0.414
0.717AspHis: 0.717 ± 0.213
5.235AspIle: 5.235 ± 0.643
6.024AspLys: 6.024 ± 0.822
4.948AspLeu: 4.948 ± 0.756
2.295AspMet: 2.295 ± 0.41
5.235AspAsn: 5.235 ± 0.807
1.004AspPro: 1.004 ± 0.281
1.506AspGln: 1.506 ± 0.42
2.151AspArg: 2.151 ± 0.343
3.37AspSer: 3.37 ± 0.494
4.733AspThr: 4.733 ± 0.704
4.374AspVal: 4.374 ± 0.565
0.717AspTrp: 0.717 ± 0.204
3.227AspTyr: 3.227 ± 0.462
0.0AspXaa: 0.0 ± 0.0
Glu
3.729GluAla: 3.729 ± 0.478
0.717GluCys: 0.717 ± 0.257
4.016GluAsp: 4.016 ± 0.663
4.589GluGlu: 4.589 ± 0.784
2.295GluPhe: 2.295 ± 0.545
3.657GluGly: 3.657 ± 0.464
1.506GluHis: 1.506 ± 0.386
5.091GluIle: 5.091 ± 0.708
5.02GluLys: 5.02 ± 0.826
6.741GluLeu: 6.741 ± 0.934
2.295GluMet: 2.295 ± 0.424
4.805GluAsn: 4.805 ± 0.874
1.721GluPro: 1.721 ± 0.323
3.299GluGln: 3.299 ± 0.428
3.37GluArg: 3.37 ± 0.546
3.299GluSer: 3.299 ± 0.603
4.374GluThr: 4.374 ± 0.586
5.665GluVal: 5.665 ± 0.765
1.291GluTrp: 1.291 ± 0.295
4.303GluTyr: 4.303 ± 0.691
0.0GluXaa: 0.0 ± 0.0
Phe
2.295PheAla: 2.295 ± 0.367
0.072PheCys: 0.072 ± 0.061
2.438PheAsp: 2.438 ± 0.418
3.442PheGlu: 3.442 ± 0.411
1.291PhePhe: 1.291 ± 0.46
3.012PheGly: 3.012 ± 0.508
0.502PheHis: 0.502 ± 0.165
3.299PheIle: 3.299 ± 0.564
3.299PheLys: 3.299 ± 0.585
2.653PheLeu: 2.653 ± 0.347
0.932PheMet: 0.932 ± 0.278
3.442PheAsn: 3.442 ± 0.512
0.43PhePro: 0.43 ± 0.181
0.717PheGln: 0.717 ± 0.219
1.147PheArg: 1.147 ± 0.289
1.721PheSer: 1.721 ± 0.412
2.438PheThr: 2.438 ± 0.411
2.582PheVal: 2.582 ± 0.435
0.143PheTrp: 0.143 ± 0.101
2.008PheTyr: 2.008 ± 0.443
0.0PheXaa: 0.0 ± 0.0
Gly
2.868GlyAla: 2.868 ± 0.733
0.359GlyCys: 0.359 ± 0.148
2.582GlyAsp: 2.582 ± 0.325
2.797GlyGlu: 2.797 ± 0.641
1.936GlyPhe: 1.936 ± 0.338
2.868GlyGly: 2.868 ± 0.459
0.861GlyHis: 0.861 ± 0.279
4.374GlyIle: 4.374 ± 0.628
5.091GlyLys: 5.091 ± 0.747
3.729GlyLeu: 3.729 ± 0.637
1.721GlyMet: 1.721 ± 0.391
3.872GlyAsn: 3.872 ± 0.49
0.717GlyPro: 0.717 ± 0.263
2.797GlyGln: 2.797 ± 0.441
3.012GlyArg: 3.012 ± 0.552
3.801GlySer: 3.801 ± 0.563
3.586GlyThr: 3.586 ± 0.409
4.733GlyVal: 4.733 ± 0.825
1.004GlyTrp: 1.004 ± 0.333
3.084GlyTyr: 3.084 ± 0.574
0.0GlyXaa: 0.0 ± 0.0
His
1.147HisAla: 1.147 ± 0.304
0.0HisCys: 0.0 ± 0.0
1.004HisAsp: 1.004 ± 0.232
0.717HisGlu: 0.717 ± 0.244
0.502HisPhe: 0.502 ± 0.164
1.076HisGly: 1.076 ± 0.309
0.287HisHis: 0.287 ± 0.146
1.864HisIle: 1.864 ± 0.325
1.362HisLys: 1.362 ± 0.329
1.793HisLeu: 1.793 ± 0.365
0.359HisMet: 0.359 ± 0.15
1.578HisAsn: 1.578 ± 0.397
0.645HisPro: 0.645 ± 0.219
0.789HisGln: 0.789 ± 0.242
0.502HisArg: 0.502 ± 0.148
0.502HisSer: 0.502 ± 0.152
1.578HisThr: 1.578 ± 0.337
1.291HisVal: 1.291 ± 0.28
0.43HisTrp: 0.43 ± 0.196
0.932HisTyr: 0.932 ± 0.316
0.0HisXaa: 0.0 ± 0.0
Ile
4.446IleAla: 4.446 ± 0.563
0.359IleCys: 0.359 ± 0.22
5.235IleAsp: 5.235 ± 0.914
5.235IleGlu: 5.235 ± 0.666
2.223IlePhe: 2.223 ± 0.443
3.944IleGly: 3.944 ± 0.552
1.147IleHis: 1.147 ± 0.276
4.231IleIle: 4.231 ± 0.575
8.175IleLys: 8.175 ± 0.841
4.303IleLeu: 4.303 ± 0.516
1.434IleMet: 1.434 ± 0.348
5.593IleAsn: 5.593 ± 0.527
2.295IlePro: 2.295 ± 0.483
2.653IleGln: 2.653 ± 0.44
3.442IleArg: 3.442 ± 0.444
4.374IleSer: 4.374 ± 0.815
4.876IleThr: 4.876 ± 0.656
4.948IleVal: 4.948 ± 1.071
1.721IleTrp: 1.721 ± 0.611
3.299IleTyr: 3.299 ± 0.564
0.0IleXaa: 0.0 ± 0.0
Lys
5.02LysAla: 5.02 ± 0.748
0.215LysCys: 0.215 ± 0.124
6.884LysAsp: 6.884 ± 0.695
7.171LysGlu: 7.171 ± 1.127
3.442LysPhe: 3.442 ± 0.503
5.45LysGly: 5.45 ± 0.632
2.151LysHis: 2.151 ± 0.464
4.231LysIle: 4.231 ± 0.587
7.601LysLys: 7.601 ± 1.032
6.669LysLeu: 6.669 ± 0.824
2.223LysMet: 2.223 ± 0.43
4.518LysAsn: 4.518 ± 0.771
2.797LysPro: 2.797 ± 0.62
4.303LysGln: 4.303 ± 0.499
4.876LysArg: 4.876 ± 0.953
4.661LysSer: 4.661 ± 0.61
5.45LysThr: 5.45 ± 0.773
5.45LysVal: 5.45 ± 0.525
0.861LysTrp: 0.861 ± 0.225
3.155LysTyr: 3.155 ± 0.451
0.0LysXaa: 0.0 ± 0.0
Leu
4.303LeuAla: 4.303 ± 0.644
0.359LeuCys: 0.359 ± 0.15
4.518LeuAsp: 4.518 ± 0.585
6.382LeuGlu: 6.382 ± 0.898
2.08LeuPhe: 2.08 ± 0.369
4.303LeuGly: 4.303 ± 0.551
1.219LeuHis: 1.219 ± 0.267
5.952LeuIle: 5.952 ± 0.618
5.307LeuLys: 5.307 ± 0.738
5.307LeuLeu: 5.307 ± 0.622
2.151LeuMet: 2.151 ± 0.41
5.307LeuAsn: 5.307 ± 0.669
2.725LeuPro: 2.725 ± 0.453
2.438LeuGln: 2.438 ± 0.441
2.725LeuArg: 2.725 ± 0.438
4.805LeuSer: 4.805 ± 0.544
4.231LeuThr: 4.231 ± 0.515
4.016LeuVal: 4.016 ± 0.462
0.717LeuTrp: 0.717 ± 0.206
2.295LeuTyr: 2.295 ± 0.46
0.0LeuXaa: 0.0 ± 0.0
Met
2.08MetAla: 2.08 ± 0.601
0.143MetCys: 0.143 ± 0.09
1.291MetAsp: 1.291 ± 0.316
1.721MetGlu: 1.721 ± 0.39
1.004MetPhe: 1.004 ± 0.282
1.362MetGly: 1.362 ± 0.468
0.717MetHis: 0.717 ± 0.22
1.649MetIle: 1.649 ± 0.359
2.725MetLys: 2.725 ± 0.363
1.793MetLeu: 1.793 ± 0.465
0.502MetMet: 0.502 ± 0.262
1.721MetAsn: 1.721 ± 0.337
0.717MetPro: 0.717 ± 0.244
1.219MetGln: 1.219 ± 0.31
1.291MetArg: 1.291 ± 0.313
1.721MetSer: 1.721 ± 0.444
1.649MetThr: 1.649 ± 0.336
1.076MetVal: 1.076 ± 0.331
0.43MetTrp: 0.43 ± 0.157
1.291MetTyr: 1.291 ± 0.294
0.0MetXaa: 0.0 ± 0.0
Asn
5.163AsnAla: 5.163 ± 0.746
0.215AsnCys: 0.215 ± 0.114
5.163AsnAsp: 5.163 ± 0.71
4.661AsnGlu: 4.661 ± 0.753
1.936AsnPhe: 1.936 ± 0.404
4.589AsnGly: 4.589 ± 0.667
1.434AsnHis: 1.434 ± 0.375
5.88AsnIle: 5.88 ± 0.646
6.239AsnLys: 6.239 ± 1.131
4.016AsnLeu: 4.016 ± 0.461
1.721AsnMet: 1.721 ± 0.316
5.235AsnAsn: 5.235 ± 0.939
1.864AsnPro: 1.864 ± 0.337
3.442AsnGln: 3.442 ± 0.611
2.868AsnArg: 2.868 ± 0.44
3.801AsnSer: 3.801 ± 0.508
4.446AsnThr: 4.446 ± 0.674
3.227AsnVal: 3.227 ± 0.495
0.932AsnTrp: 0.932 ± 0.291
3.227AsnTyr: 3.227 ± 0.562
0.0AsnXaa: 0.0 ± 0.0
Pro
0.861ProAla: 0.861 ± 0.296
0.143ProCys: 0.143 ± 0.087
2.008ProAsp: 2.008 ± 0.448
1.434ProGlu: 1.434 ± 0.275
1.434ProPhe: 1.434 ± 0.388
0.861ProGly: 0.861 ± 0.258
0.789ProHis: 0.789 ± 0.244
1.936ProIle: 1.936 ± 0.434
2.797ProLys: 2.797 ± 0.533
2.223ProLeu: 2.223 ± 0.488
0.43ProMet: 0.43 ± 0.164
1.793ProAsn: 1.793 ± 0.29
0.645ProPro: 0.645 ± 0.257
1.362ProGln: 1.362 ± 0.32
1.219ProArg: 1.219 ± 0.305
2.08ProSer: 2.08 ± 0.488
2.51ProThr: 2.51 ± 0.497
1.434ProVal: 1.434 ± 0.324
0.0ProTrp: 0.0 ± 0.0
1.291ProTyr: 1.291 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
2.94GlnAla: 2.94 ± 0.677
0.359GlnCys: 0.359 ± 0.153
2.582GlnAsp: 2.582 ± 0.516
3.299GlnGlu: 3.299 ± 0.612
1.649GlnPhe: 1.649 ± 0.293
1.721GlnGly: 1.721 ± 0.345
0.502GlnHis: 0.502 ± 0.175
2.797GlnIle: 2.797 ± 0.436
2.797GlnLys: 2.797 ± 0.413
2.438GlnLeu: 2.438 ± 0.352
1.219GlnMet: 1.219 ± 0.284
2.94GlnAsn: 2.94 ± 0.604
1.578GlnPro: 1.578 ± 0.362
3.084GlnGln: 3.084 ± 0.864
2.51GlnArg: 2.51 ± 0.408
2.51GlnSer: 2.51 ± 0.443
2.008GlnThr: 2.008 ± 0.345
2.438GlnVal: 2.438 ± 0.431
0.43GlnTrp: 0.43 ± 0.155
1.793GlnTyr: 1.793 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
1.936ArgAla: 1.936 ± 0.321
0.287ArgCys: 0.287 ± 0.141
3.155ArgAsp: 3.155 ± 0.385
2.868ArgGlu: 2.868 ± 0.505
1.506ArgPhe: 1.506 ± 0.391
2.295ArgGly: 2.295 ± 0.382
1.362ArgHis: 1.362 ± 0.362
3.586ArgIle: 3.586 ± 0.495
3.801ArgLys: 3.801 ± 0.571
3.586ArgLeu: 3.586 ± 0.662
1.721ArgMet: 1.721 ± 0.4
2.653ArgAsn: 2.653 ± 0.586
1.147ArgPro: 1.147 ± 0.267
1.578ArgGln: 1.578 ± 0.33
2.868ArgArg: 2.868 ± 0.702
2.797ArgSer: 2.797 ± 0.337
1.936ArgThr: 1.936 ± 0.414
2.366ArgVal: 2.366 ± 0.442
0.502ArgTrp: 0.502 ± 0.164
2.797ArgTyr: 2.797 ± 0.587
0.0ArgXaa: 0.0 ± 0.0
Ser
3.801SerAla: 3.801 ± 1.242
0.359SerCys: 0.359 ± 0.162
4.446SerAsp: 4.446 ± 0.593
3.729SerGlu: 3.729 ± 0.493
2.94SerPhe: 2.94 ± 0.525
3.944SerGly: 3.944 ± 0.673
1.219SerHis: 1.219 ± 0.289
5.235SerIle: 5.235 ± 0.884
5.235SerLys: 5.235 ± 0.768
4.446SerLeu: 4.446 ± 0.553
1.362SerMet: 1.362 ± 0.23
3.801SerAsn: 3.801 ± 0.522
1.506SerPro: 1.506 ± 0.34
1.578SerGln: 1.578 ± 0.271
2.008SerArg: 2.008 ± 0.325
3.586SerSer: 3.586 ± 0.657
4.374SerThr: 4.374 ± 0.413
3.801SerVal: 3.801 ± 0.51
0.502SerTrp: 0.502 ± 0.138
2.653SerTyr: 2.653 ± 0.509
0.0SerXaa: 0.0 ± 0.0
Thr
3.37ThrAla: 3.37 ± 0.409
0.215ThrCys: 0.215 ± 0.119
3.514ThrAsp: 3.514 ± 0.515
3.944ThrGlu: 3.944 ± 0.602
2.582ThrPhe: 2.582 ± 0.456
3.012ThrGly: 3.012 ± 0.526
1.147ThrHis: 1.147 ± 0.282
5.02ThrIle: 5.02 ± 0.579
5.522ThrLys: 5.522 ± 0.84
4.876ThrLeu: 4.876 ± 0.698
1.004ThrMet: 1.004 ± 0.249
5.307ThrAsn: 5.307 ± 0.707
2.438ThrPro: 2.438 ± 0.383
2.438ThrGln: 2.438 ± 0.421
1.793ThrArg: 1.793 ± 0.315
4.303ThrSer: 4.303 ± 0.513
4.876ThrThr: 4.876 ± 0.792
4.374ThrVal: 4.374 ± 0.739
0.645ThrTrp: 0.645 ± 0.203
3.012ThrTyr: 3.012 ± 0.404
0.0ThrXaa: 0.0 ± 0.0
Val
4.446ValAla: 4.446 ± 1.137
0.502ValCys: 0.502 ± 0.183
4.805ValAsp: 4.805 ± 0.538
5.163ValGlu: 5.163 ± 0.722
1.936ValPhe: 1.936 ± 0.385
2.725ValGly: 2.725 ± 0.502
0.43ValHis: 0.43 ± 0.204
3.872ValIle: 3.872 ± 0.708
6.239ValLys: 6.239 ± 0.702
3.872ValLeu: 3.872 ± 0.562
1.362ValMet: 1.362 ± 0.362
3.37ValAsn: 3.37 ± 0.383
2.295ValPro: 2.295 ± 0.502
2.94ValGln: 2.94 ± 0.52
3.37ValArg: 3.37 ± 0.443
4.948ValSer: 4.948 ± 0.901
4.374ValThr: 4.374 ± 0.7
3.872ValVal: 3.872 ± 0.955
0.645ValTrp: 0.645 ± 0.179
1.793ValTyr: 1.793 ± 0.476
0.0ValXaa: 0.0 ± 0.0
Trp
0.861TrpAla: 0.861 ± 0.258
0.359TrpCys: 0.359 ± 0.148
0.861TrpAsp: 0.861 ± 0.262
0.861TrpGlu: 0.861 ± 0.283
0.502TrpPhe: 0.502 ± 0.263
0.574TrpGly: 0.574 ± 0.219
0.359TrpHis: 0.359 ± 0.159
1.004TrpIle: 1.004 ± 0.249
0.789TrpLys: 0.789 ± 0.195
0.932TrpLeu: 0.932 ± 0.309
0.143TrpMet: 0.143 ± 0.106
0.932TrpAsn: 0.932 ± 0.267
0.0TrpPro: 0.0 ± 0.0
0.717TrpGln: 0.717 ± 0.277
0.43TrpArg: 0.43 ± 0.171
1.219TrpSer: 1.219 ± 0.325
1.004TrpThr: 1.004 ± 0.469
0.502TrpVal: 0.502 ± 0.17
0.0TrpTrp: 0.0 ± 0.0
0.574TrpTyr: 0.574 ± 0.236
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.438TyrAla: 2.438 ± 0.473
0.43TyrCys: 0.43 ± 0.2
2.653TyrAsp: 2.653 ± 0.508
4.087TyrGlu: 4.087 ± 0.636
1.649TyrPhe: 1.649 ± 0.352
2.725TyrGly: 2.725 ± 0.492
0.502TyrHis: 0.502 ± 0.162
3.299TyrIle: 3.299 ± 0.56
3.729TyrLys: 3.729 ± 0.62
2.725TyrLeu: 2.725 ± 0.495
1.219TyrMet: 1.219 ± 0.3
3.514TyrAsn: 3.514 ± 0.557
1.004TyrPro: 1.004 ± 0.264
2.08TyrGln: 2.08 ± 0.412
1.936TyrArg: 1.936 ± 0.393
3.514TyrSer: 3.514 ± 0.554
2.295TyrThr: 2.295 ± 0.355
2.51TyrVal: 2.51 ± 0.442
1.004TyrTrp: 1.004 ± 0.271
1.578TyrTyr: 1.578 ± 0.344
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (13946 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski