Streptomyces phage Nesbitt

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Abbeymikolonvirus; unclassified Abbeymikolonvirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1JT29|A0A2P1JT29_9CAUD RNA polymerase sigma factor OS=Streptomyces phage Nesbitt OX=2108133 GN=43 PE=4 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84TPITTTNYY10 pKa = 9.08FRR12 pKa = 11.84QGAAASAVMLAAMATLAGCDD32 pKa = 4.5DD33 pKa = 4.27GPEE36 pKa = 4.16CLDD39 pKa = 3.66YY40 pKa = 10.01DD41 pKa = 4.16TQVVSTTSIVNGRR54 pKa = 11.84VVPGMTTVTVCTKK67 pKa = 10.06YY68 pKa = 10.75AEE70 pKa = 4.67PSTTPSEE77 pKa = 4.23

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1JT11|A0A2P1JT11_9CAUD Uncharacterized protein OS=Streptomyces phage Nesbitt OX=2108133 GN=7 PE=4 SV=1
MM1 pKa = 7.89TDD3 pKa = 2.94NQRR6 pKa = 11.84RR7 pKa = 11.84TARR10 pKa = 11.84TVFQTAVTLAAAVPALVEE28 pKa = 4.07ASGLAQTSGAVVIALAVSGAVTRR51 pKa = 11.84IMALPVVDD59 pKa = 3.98QLLPGWLRR67 pKa = 11.84KK68 pKa = 8.45EE69 pKa = 4.01AA70 pKa = 3.94

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12530

43

1045

216.0

23.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.548 ± 0.363

0.766 ± 0.115

6.225 ± 0.234

6.457 ± 0.37

2.785 ± 0.184

8.835 ± 0.381

1.636 ± 0.197

3.432 ± 0.183

4.413 ± 0.406

8.5 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.12

2.57 ± 0.154

5.235 ± 0.237

2.817 ± 0.162

7.63 ± 0.431

5.227 ± 0.24

6.927 ± 0.341

7.941 ± 0.242

1.899 ± 0.18

2.785 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski