Amino acid dipepetide frequency for Streptomyces phage Nesbitt

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.371AlaAla: 12.371 ± 1.155
0.239AlaCys: 0.239 ± 0.126
7.662AlaAsp: 7.662 ± 0.793
7.423AlaGlu: 7.423 ± 0.863
2.075AlaPhe: 2.075 ± 0.483
9.019AlaGly: 9.019 ± 0.881
1.836AlaHis: 1.836 ± 0.505
3.751AlaIle: 3.751 ± 0.679
6.465AlaLys: 6.465 ± 1.015
12.93AlaLeu: 12.93 ± 1.038
3.352AlaMet: 3.352 ± 0.529
3.592AlaAsn: 3.592 ± 0.534
4.789AlaPro: 4.789 ± 0.578
3.352AlaGln: 3.352 ± 0.467
7.503AlaArg: 7.503 ± 0.74
6.704AlaSer: 6.704 ± 0.821
7.343AlaThr: 7.343 ± 1.083
9.418AlaVal: 9.418 ± 0.937
2.474AlaTrp: 2.474 ± 0.428
3.113AlaTyr: 3.113 ± 0.452
0.0AlaXaa: 0.0 ± 0.0
Cys
0.639CysAla: 0.639 ± 0.209
0.239CysCys: 0.239 ± 0.161
0.718CysAsp: 0.718 ± 0.246
0.239CysGlu: 0.239 ± 0.12
0.16CysPhe: 0.16 ± 0.114
0.798CysGly: 0.798 ± 0.277
0.239CysHis: 0.239 ± 0.192
0.16CysIle: 0.16 ± 0.101
0.319CysLys: 0.319 ± 0.148
0.878CysLeu: 0.878 ± 0.372
0.16CysMet: 0.16 ± 0.128
0.08CysAsn: 0.08 ± 0.079
0.798CysPro: 0.798 ± 0.252
0.639CysGln: 0.639 ± 0.212
0.639CysArg: 0.639 ± 0.247
0.08CysSer: 0.08 ± 0.084
0.479CysThr: 0.479 ± 0.2
0.16CysVal: 0.16 ± 0.101
0.08CysTrp: 0.08 ± 0.097
0.16CysTyr: 0.16 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
6.944AspAla: 6.944 ± 0.748
0.798AspCys: 0.798 ± 0.231
2.953AspAsp: 2.953 ± 0.554
2.953AspGlu: 2.953 ± 0.582
1.277AspPhe: 1.277 ± 0.322
7.503AspGly: 7.503 ± 0.891
0.958AspHis: 0.958 ± 0.229
2.155AspIle: 2.155 ± 0.351
1.596AspLys: 1.596 ± 0.397
5.986AspLeu: 5.986 ± 0.817
1.277AspMet: 1.277 ± 0.317
1.676AspAsn: 1.676 ± 0.319
4.709AspPro: 4.709 ± 0.679
2.315AspGln: 2.315 ± 0.323
4.31AspArg: 4.31 ± 0.651
2.155AspSer: 2.155 ± 0.459
4.23AspThr: 4.23 ± 0.535
5.427AspVal: 5.427 ± 0.532
1.357AspTrp: 1.357 ± 0.285
1.676AspTyr: 1.676 ± 0.355
0.0AspXaa: 0.0 ± 0.0
Glu
9.578GluAla: 9.578 ± 0.992
0.559GluCys: 0.559 ± 0.189
4.709GluAsp: 4.709 ± 0.806
3.751GluGlu: 3.751 ± 0.597
2.794GluPhe: 2.794 ± 0.442
6.465GluGly: 6.465 ± 0.969
1.038GluHis: 1.038 ± 0.331
2.554GluIle: 2.554 ± 0.451
0.958GluLys: 0.958 ± 0.356
4.949GluLeu: 4.949 ± 0.662
1.995GluMet: 1.995 ± 0.337
1.277GluAsn: 1.277 ± 0.313
2.315GluPro: 2.315 ± 0.406
0.399GluGln: 0.399 ± 0.141
5.747GluArg: 5.747 ± 0.901
2.794GluSer: 2.794 ± 0.585
4.949GluThr: 4.949 ± 0.655
5.028GluVal: 5.028 ± 0.852
1.038GluTrp: 1.038 ± 0.31
1.676GluTyr: 1.676 ± 0.407
0.0GluXaa: 0.0 ± 0.0
Phe
2.794PheAla: 2.794 ± 0.591
0.16PheCys: 0.16 ± 0.104
1.596PheAsp: 1.596 ± 0.309
1.437PheGlu: 1.437 ± 0.337
0.399PhePhe: 0.399 ± 0.212
2.953PheGly: 2.953 ± 0.618
0.239PheHis: 0.239 ± 0.133
1.038PheIle: 1.038 ± 0.306
1.756PheLys: 1.756 ± 0.44
1.676PheLeu: 1.676 ± 0.337
0.958PheMet: 0.958 ± 0.263
0.798PheAsn: 0.798 ± 0.321
1.756PhePro: 1.756 ± 0.402
0.958PheGln: 0.958 ± 0.35
3.352PheArg: 3.352 ± 0.415
0.878PheSer: 0.878 ± 0.301
1.995PheThr: 1.995 ± 0.316
1.596PheVal: 1.596 ± 0.348
0.16PheTrp: 0.16 ± 0.1
1.357PheTyr: 1.357 ± 0.423
0.0PheXaa: 0.0 ± 0.0
Gly
9.977GlyAla: 9.977 ± 0.907
0.479GlyCys: 0.479 ± 0.171
5.268GlyAsp: 5.268 ± 0.728
5.826GlyGlu: 5.826 ± 0.831
2.634GlyPhe: 2.634 ± 0.6
6.784GlyGly: 6.784 ± 1.123
1.038GlyHis: 1.038 ± 0.245
3.352GlyIle: 3.352 ± 0.76
4.071GlyLys: 4.071 ± 0.914
8.221GlyLeu: 8.221 ± 0.729
2.075GlyMet: 2.075 ± 0.386
1.995GlyAsn: 1.995 ± 0.39
3.592GlyPro: 3.592 ± 0.616
3.033GlyGln: 3.033 ± 0.408
6.385GlyArg: 6.385 ± 0.551
5.667GlySer: 5.667 ± 0.684
5.507GlyThr: 5.507 ± 0.758
7.822GlyVal: 7.822 ± 0.714
2.075GlyTrp: 2.075 ± 0.48
2.554GlyTyr: 2.554 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
1.836HisAla: 1.836 ± 0.318
0.08HisCys: 0.08 ± 0.074
0.718HisAsp: 0.718 ± 0.208
1.117HisGlu: 1.117 ± 0.345
0.399HisPhe: 0.399 ± 0.164
1.357HisGly: 1.357 ± 0.4
0.559HisHis: 0.559 ± 0.215
1.038HisIle: 1.038 ± 0.279
0.718HisLys: 0.718 ± 0.248
1.197HisLeu: 1.197 ± 0.452
0.319HisMet: 0.319 ± 0.168
0.559HisAsn: 0.559 ± 0.242
0.878HisPro: 0.878 ± 0.277
0.399HisGln: 0.399 ± 0.158
1.197HisArg: 1.197 ± 0.404
0.479HisSer: 0.479 ± 0.18
1.437HisThr: 1.437 ± 0.351
1.516HisVal: 1.516 ± 0.414
0.399HisTrp: 0.399 ± 0.153
0.16HisTyr: 0.16 ± 0.098
0.0HisXaa: 0.0 ± 0.0
Ile
3.751IleAla: 3.751 ± 0.847
0.08IleCys: 0.08 ± 0.073
2.155IleAsp: 2.155 ± 0.458
0.798IleGlu: 0.798 ± 0.243
1.038IlePhe: 1.038 ± 0.313
2.554IleGly: 2.554 ± 0.541
1.197IleHis: 1.197 ± 0.321
0.798IleIle: 0.798 ± 0.222
1.676IleLys: 1.676 ± 0.332
2.873IleLeu: 2.873 ± 0.566
0.479IleMet: 0.479 ± 0.173
0.718IleAsn: 0.718 ± 0.258
1.596IlePro: 1.596 ± 0.368
0.878IleGln: 0.878 ± 0.272
4.789IleArg: 4.789 ± 0.695
1.437IleSer: 1.437 ± 0.4
3.512IleThr: 3.512 ± 0.559
2.953IleVal: 2.953 ± 0.613
0.479IleTrp: 0.479 ± 0.172
0.559IleTyr: 0.559 ± 0.21
0.0IleXaa: 0.0 ± 0.0
Lys
6.226LysAla: 6.226 ± 1.064
0.239LysCys: 0.239 ± 0.142
3.272LysAsp: 3.272 ± 0.94
3.512LysGlu: 3.512 ± 0.63
1.117LysPhe: 1.117 ± 0.273
4.39LysGly: 4.39 ± 0.863
0.639LysHis: 0.639 ± 0.203
0.878LysIle: 0.878 ± 0.333
1.516LysLys: 1.516 ± 0.339
3.592LysLeu: 3.592 ± 0.526
1.117LysMet: 1.117 ± 0.294
1.038LysAsn: 1.038 ± 0.249
2.634LysPro: 2.634 ± 0.544
0.639LysGln: 0.639 ± 0.201
3.432LysArg: 3.432 ± 0.521
2.235LysSer: 2.235 ± 0.394
2.315LysThr: 2.315 ± 0.354
3.512LysVal: 3.512 ± 0.687
0.718LysTrp: 0.718 ± 0.258
1.117LysTyr: 1.117 ± 0.257
0.0LysXaa: 0.0 ± 0.0
Leu
10.456LeuAla: 10.456 ± 1.143
1.277LeuCys: 1.277 ± 0.356
4.23LeuAsp: 4.23 ± 0.659
6.784LeuGlu: 6.784 ± 0.957
1.916LeuPhe: 1.916 ± 0.389
6.784LeuGly: 6.784 ± 0.787
1.596LeuHis: 1.596 ± 0.341
2.714LeuIle: 2.714 ± 0.401
3.272LeuLys: 3.272 ± 0.548
5.826LeuLeu: 5.826 ± 0.581
1.516LeuMet: 1.516 ± 0.495
1.596LeuAsn: 1.596 ± 0.307
4.549LeuPro: 4.549 ± 0.53
2.953LeuGln: 2.953 ± 0.401
6.784LeuArg: 6.784 ± 0.879
4.39LeuSer: 4.39 ± 0.524
6.784LeuThr: 6.784 ± 0.655
7.183LeuVal: 7.183 ± 0.7
2.155LeuTrp: 2.155 ± 0.428
2.235LeuTyr: 2.235 ± 0.417
0.0LeuXaa: 0.0 ± 0.0
Met
4.39MetAla: 4.39 ± 0.604
0.08MetCys: 0.08 ± 0.078
1.516MetAsp: 1.516 ± 0.496
1.516MetGlu: 1.516 ± 0.361
0.559MetPhe: 0.559 ± 0.22
1.916MetGly: 1.916 ± 0.458
0.16MetHis: 0.16 ± 0.124
0.559MetIle: 0.559 ± 0.245
0.878MetLys: 0.878 ± 0.323
1.516MetLeu: 1.516 ± 0.429
0.16MetMet: 0.16 ± 0.102
0.479MetAsn: 0.479 ± 0.217
0.958MetPro: 0.958 ± 0.275
0.479MetGln: 0.479 ± 0.187
1.836MetArg: 1.836 ± 0.4
1.596MetSer: 1.596 ± 0.332
2.155MetThr: 2.155 ± 0.568
2.394MetVal: 2.394 ± 0.442
0.399MetTrp: 0.399 ± 0.21
0.16MetTyr: 0.16 ± 0.101
0.0MetXaa: 0.0 ± 0.0
Asn
3.512AsnAla: 3.512 ± 0.426
0.0AsnCys: 0.0 ± 0.0
1.038AsnAsp: 1.038 ± 0.274
0.878AsnGlu: 0.878 ± 0.261
0.399AsnPhe: 0.399 ± 0.178
3.592AsnGly: 3.592 ± 0.603
0.399AsnHis: 0.399 ± 0.219
0.718AsnIle: 0.718 ± 0.25
1.676AsnLys: 1.676 ± 0.502
2.315AsnLeu: 2.315 ± 0.385
0.16AsnMet: 0.16 ± 0.113
0.479AsnAsn: 0.479 ± 0.203
2.953AsnPro: 2.953 ± 0.428
0.718AsnGln: 0.718 ± 0.198
1.357AsnArg: 1.357 ± 0.384
0.958AsnSer: 0.958 ± 0.281
1.437AsnThr: 1.437 ± 0.327
2.155AsnVal: 2.155 ± 0.315
0.399AsnTrp: 0.399 ± 0.211
0.559AsnTyr: 0.559 ± 0.208
0.0AsnXaa: 0.0 ± 0.0
Pro
5.348ProAla: 5.348 ± 0.673
0.479ProCys: 0.479 ± 0.209
3.512ProAsp: 3.512 ± 0.595
4.789ProGlu: 4.789 ± 0.626
1.995ProPhe: 1.995 ± 0.37
5.108ProGly: 5.108 ± 0.634
0.878ProHis: 0.878 ± 0.337
1.277ProIle: 1.277 ± 0.301
2.554ProLys: 2.554 ± 0.515
4.15ProLeu: 4.15 ± 0.646
1.437ProMet: 1.437 ± 0.354
1.516ProAsn: 1.516 ± 0.317
2.394ProPro: 2.394 ± 0.455
1.357ProGln: 1.357 ± 0.402
2.634ProArg: 2.634 ± 0.478
2.953ProSer: 2.953 ± 0.501
2.714ProThr: 2.714 ± 0.366
4.949ProVal: 4.949 ± 0.51
0.639ProTrp: 0.639 ± 0.197
1.676ProTyr: 1.676 ± 0.365
0.0ProXaa: 0.0 ± 0.0
Gln
3.831GlnAla: 3.831 ± 0.651
0.559GlnCys: 0.559 ± 0.206
1.197GlnAsp: 1.197 ± 0.34
2.634GlnGlu: 2.634 ± 0.418
0.718GlnPhe: 0.718 ± 0.26
3.193GlnGly: 3.193 ± 0.616
0.399GlnHis: 0.399 ± 0.176
0.479GlnIle: 0.479 ± 0.182
0.639GlnLys: 0.639 ± 0.27
1.596GlnLeu: 1.596 ± 0.374
0.958GlnMet: 0.958 ± 0.308
0.798GlnAsn: 0.798 ± 0.35
0.878GlnPro: 0.878 ± 0.299
0.239GlnGln: 0.239 ± 0.121
3.113GlnArg: 3.113 ± 0.527
0.958GlnSer: 0.958 ± 0.279
2.394GlnThr: 2.394 ± 0.462
1.995GlnVal: 1.995 ± 0.452
0.639GlnTrp: 0.639 ± 0.275
0.958GlnTyr: 0.958 ± 0.253
0.0GlnXaa: 0.0 ± 0.0
Arg
7.024ArgAla: 7.024 ± 0.798
0.639ArgCys: 0.639 ± 0.269
4.549ArgAsp: 4.549 ± 0.679
4.949ArgGlu: 4.949 ± 0.947
1.836ArgPhe: 1.836 ± 0.431
5.906ArgGly: 5.906 ± 0.609
1.437ArgHis: 1.437 ± 0.421
3.751ArgIle: 3.751 ± 0.738
3.911ArgLys: 3.911 ± 0.537
7.343ArgLeu: 7.343 ± 0.949
2.235ArgMet: 2.235 ± 0.428
1.836ArgAsn: 1.836 ± 0.42
4.47ArgPro: 4.47 ± 0.637
2.714ArgGln: 2.714 ± 0.556
6.864ArgArg: 6.864 ± 1.274
3.352ArgSer: 3.352 ± 0.64
4.47ArgThr: 4.47 ± 0.548
5.028ArgVal: 5.028 ± 0.795
1.836ArgTrp: 1.836 ± 0.421
2.155ArgTyr: 2.155 ± 0.394
0.0ArgXaa: 0.0 ± 0.0
Ser
5.587SerAla: 5.587 ± 0.601
0.16SerCys: 0.16 ± 0.125
3.193SerAsp: 3.193 ± 0.53
3.113SerGlu: 3.113 ± 0.488
1.596SerPhe: 1.596 ± 0.38
5.507SerGly: 5.507 ± 0.819
0.958SerHis: 0.958 ± 0.263
2.634SerIle: 2.634 ± 0.637
3.512SerLys: 3.512 ± 0.608
4.949SerLeu: 4.949 ± 0.577
1.516SerMet: 1.516 ± 0.352
1.357SerAsn: 1.357 ± 0.273
1.756SerPro: 1.756 ± 0.43
1.038SerGln: 1.038 ± 0.371
3.033SerArg: 3.033 ± 0.436
2.714SerSer: 2.714 ± 0.516
3.592SerThr: 3.592 ± 0.587
3.432SerVal: 3.432 ± 0.606
1.277SerTrp: 1.277 ± 0.384
1.357SerTyr: 1.357 ± 0.275
0.0SerXaa: 0.0 ± 0.0
Thr
7.183ThrAla: 7.183 ± 0.636
0.399ThrCys: 0.399 ± 0.151
5.826ThrAsp: 5.826 ± 0.645
4.629ThrGlu: 4.629 ± 0.548
3.193ThrPhe: 3.193 ± 0.618
5.747ThrGly: 5.747 ± 0.727
0.958ThrHis: 0.958 ± 0.347
1.995ThrIle: 1.995 ± 0.452
2.714ThrLys: 2.714 ± 0.445
5.587ThrLeu: 5.587 ± 0.596
1.117ThrMet: 1.117 ± 0.301
1.756ThrAsn: 1.756 ± 0.355
3.751ThrPro: 3.751 ± 0.718
1.836ThrGln: 1.836 ± 0.33
4.31ThrArg: 4.31 ± 0.692
4.071ThrSer: 4.071 ± 0.691
4.629ThrThr: 4.629 ± 0.961
5.507ThrVal: 5.507 ± 0.747
1.038ThrTrp: 1.038 ± 0.343
3.033ThrTyr: 3.033 ± 0.591
0.0ThrXaa: 0.0 ± 0.0
Val
8.939ValAla: 8.939 ± 0.82
0.559ValCys: 0.559 ± 0.221
5.348ValAsp: 5.348 ± 0.59
5.507ValGlu: 5.507 ± 0.644
2.235ValPhe: 2.235 ± 0.357
4.31ValGly: 4.31 ± 0.667
1.197ValHis: 1.197 ± 0.353
3.352ValIle: 3.352 ± 0.519
3.991ValLys: 3.991 ± 0.555
5.348ValLeu: 5.348 ± 0.566
2.075ValMet: 2.075 ± 0.371
2.714ValAsn: 2.714 ± 0.5
5.028ValPro: 5.028 ± 0.578
2.953ValGln: 2.953 ± 0.554
5.268ValArg: 5.268 ± 0.801
5.826ValSer: 5.826 ± 0.761
6.465ValThr: 6.465 ± 0.725
4.629ValVal: 4.629 ± 0.578
1.277ValTrp: 1.277 ± 0.355
2.394ValTyr: 2.394 ± 0.429
0.0ValXaa: 0.0 ± 0.0
Trp
1.676TrpAla: 1.676 ± 0.431
0.399TrpCys: 0.399 ± 0.156
1.117TrpAsp: 1.117 ± 0.236
1.596TrpGlu: 1.596 ± 0.34
0.639TrpPhe: 0.639 ± 0.195
1.916TrpGly: 1.916 ± 0.425
0.559TrpHis: 0.559 ± 0.205
0.239TrpIle: 0.239 ± 0.13
0.958TrpLys: 0.958 ± 0.391
1.836TrpLeu: 1.836 ± 0.642
0.399TrpMet: 0.399 ± 0.18
0.639TrpAsn: 0.639 ± 0.253
0.559TrpPro: 0.559 ± 0.231
0.479TrpGln: 0.479 ± 0.168
1.836TrpArg: 1.836 ± 0.391
1.357TrpSer: 1.357 ± 0.324
0.718TrpThr: 0.718 ± 0.223
1.357TrpVal: 1.357 ± 0.322
0.399TrpTrp: 0.399 ± 0.202
0.319TrpTyr: 0.319 ± 0.161
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.432TyrAla: 3.432 ± 0.552
0.239TyrCys: 0.239 ± 0.139
1.676TyrAsp: 1.676 ± 0.339
1.117TyrGlu: 1.117 ± 0.281
0.878TyrPhe: 0.878 ± 0.26
2.554TyrGly: 2.554 ± 0.472
0.08TyrHis: 0.08 ± 0.103
0.878TyrIle: 0.878 ± 0.307
0.958TyrLys: 0.958 ± 0.305
2.235TyrLeu: 2.235 ± 0.347
0.319TyrMet: 0.319 ± 0.144
0.798TyrAsn: 0.798 ± 0.237
1.995TyrPro: 1.995 ± 0.561
0.798TyrGln: 0.798 ± 0.233
1.916TyrArg: 1.916 ± 0.4
1.676TyrSer: 1.676 ± 0.457
2.155TyrThr: 2.155 ± 0.441
3.352TyrVal: 3.352 ± 0.696
0.16TyrTrp: 0.16 ± 0.106
0.639TyrTyr: 0.639 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12530 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski