Arthrobacter phage Anjali

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Anjalivirus; Arthrobacter virus Anjali

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3LXW7|A0A3G3LXW7_9CAUD Uncharacterized protein OS=Arthrobacter phage Anjali OX=2484217 GN=24 PE=4 SV=1
MM1 pKa = 6.74VTTATGVFPYY11 pKa = 10.47RR12 pKa = 11.84LQPLNNISAFTYY24 pKa = 8.76RR25 pKa = 11.84TGVTYY30 pKa = 11.08AEE32 pKa = 4.22MLEE35 pKa = 4.03EE36 pKa = 3.91LRR38 pKa = 11.84VYY40 pKa = 10.71INDD43 pKa = 3.43TMRR46 pKa = 11.84PEE48 pKa = 4.2FNSVILSVLNDD59 pKa = 3.74FQAGIEE65 pKa = 4.1NAEE68 pKa = 4.08TRR70 pKa = 11.84VVQSEE75 pKa = 4.84TEE77 pKa = 4.18FTALMEE83 pKa = 4.54DD84 pKa = 2.86ARR86 pKa = 11.84AYY88 pKa = 10.95VDD90 pKa = 4.78ASVQFINNKK99 pKa = 8.31TGQAQIQRR107 pKa = 11.84VTLTAPYY114 pKa = 9.51TVTIDD119 pKa = 3.7PLWPNNHH126 pKa = 7.57PINIVLTQDD135 pKa = 2.84ATGGRR140 pKa = 11.84TVTFDD145 pKa = 3.75AEE147 pKa = 4.84DD148 pKa = 3.36INGTVPVNTAPNAEE162 pKa = 3.97TDD164 pKa = 3.64FWLVPEE170 pKa = 5.39GDD172 pKa = 4.18GTWTAVQYY180 pKa = 8.56VTATQLAGEE189 pKa = 4.36VTTINTALGTKK200 pKa = 10.05ANSSSLATKK209 pKa = 10.12ADD211 pKa = 3.27KK212 pKa = 10.26ATTITSSAPLYY223 pKa = 10.66AVAGVLRR230 pKa = 11.84NTGSGWNWISDD241 pKa = 3.65AGHH244 pKa = 6.26EE245 pKa = 4.37PIGIDD250 pKa = 3.56SVSSDD255 pKa = 2.97SSAITVNYY263 pKa = 7.2GTLGASKK270 pKa = 9.73VGSFIVVPDD279 pKa = 4.04EE280 pKa = 4.21KK281 pKa = 10.9LSEE284 pKa = 3.96QGFQAGASVGTTSTDD299 pKa = 2.66IYY301 pKa = 10.25LSRR304 pKa = 11.84SIPSYY309 pKa = 10.5SDD311 pKa = 3.21YY312 pKa = 11.25VAYY315 pKa = 10.63SSGAFVSANGVFTCTWNATDD335 pKa = 5.22GYY337 pKa = 10.64LQVTHH342 pKa = 6.02PTIIGSALNVSLAGRR357 pKa = 11.84PGTALNYY364 pKa = 8.54TPVVAGDD371 pKa = 3.86LTTGSTFIAIEE382 pKa = 3.97FRR384 pKa = 11.84DD385 pKa = 3.56AAGNQVMTPDD395 pKa = 2.86TNMRR399 pKa = 11.84VFVTHH404 pKa = 6.71GGGFVPSVDD413 pKa = 3.29PSTVTTATYY422 pKa = 10.01PNSNLWVMGLMRR434 pKa = 11.84DD435 pKa = 3.36

Molecular weight: 46.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3LXW5|A0A3G3LXW5_9CAUD Minor tail protein OS=Arthrobacter phage Anjali OX=2484217 GN=14 PE=4 SV=1
MM1 pKa = 7.48SRR3 pKa = 11.84SCRR6 pKa = 11.84NTRR9 pKa = 11.84EE10 pKa = 4.02NQSMQTAALKK20 pKa = 10.75RR21 pKa = 11.84EE22 pKa = 4.34AEE24 pKa = 4.03QAAQRR29 pKa = 11.84IEE31 pKa = 3.68WTGNIKK37 pKa = 10.26VYY39 pKa = 10.92YY40 pKa = 8.82PAAAKK45 pKa = 10.2

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

5894

40

733

218.3

23.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.333 ± 0.474

0.747 ± 0.172

6.176 ± 0.32

5.192 ± 0.547

3.834 ± 0.428

8.127 ± 0.73

1.849 ± 0.241

5.395 ± 0.267

4.411 ± 0.583

7.109 ± 0.406

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.245

5.344 ± 0.443

5.005 ± 0.291

3.919 ± 0.295

5.039 ± 0.535

5.175 ± 0.337

8.161 ± 0.705

6.668 ± 0.574

1.612 ± 0.131

3.58 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski