Amino acid dipepetide frequency for Arthrobacter phage Anjali

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.182AlaAla: 10.182 ± 1.892
1.358AlaCys: 1.358 ± 0.385
5.77AlaAsp: 5.77 ± 0.941
4.751AlaGlu: 4.751 ± 0.885
3.564AlaPhe: 3.564 ± 0.693
9.672AlaGly: 9.672 ± 1.54
1.867AlaHis: 1.867 ± 0.524
5.26AlaIle: 5.26 ± 0.881
5.77AlaLys: 5.77 ± 1.035
6.957AlaLeu: 6.957 ± 1.127
2.376AlaMet: 2.376 ± 0.743
6.448AlaAsn: 6.448 ± 1.214
4.412AlaPro: 4.412 ± 0.616
3.733AlaGln: 3.733 ± 0.98
5.091AlaArg: 5.091 ± 0.84
5.26AlaSer: 5.26 ± 0.928
8.145AlaThr: 8.145 ± 1.598
7.127AlaVal: 7.127 ± 1.182
1.018AlaTrp: 1.018 ± 0.489
4.582AlaTyr: 4.582 ± 1.032
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.848CysAsp: 0.848 ± 0.454
0.509CysGlu: 0.509 ± 0.26
0.17CysPhe: 0.17 ± 0.166
0.679CysGly: 0.679 ± 0.527
0.17CysHis: 0.17 ± 0.199
0.17CysIle: 0.17 ± 0.166
0.17CysLys: 0.17 ± 0.175
0.339CysLeu: 0.339 ± 0.238
0.17CysMet: 0.17 ± 0.184
0.17CysAsn: 0.17 ± 0.183
0.679CysPro: 0.679 ± 0.295
0.0CysGln: 0.0 ± 0.0
0.848CysArg: 0.848 ± 0.408
0.339CysSer: 0.339 ± 0.366
0.848CysThr: 0.848 ± 0.391
0.679CysVal: 0.679 ± 0.409
0.17CysTrp: 0.17 ± 0.16
0.509CysTyr: 0.509 ± 0.239
0.0CysXaa: 0.0 ± 0.0
Asp
7.636AspAla: 7.636 ± 1.234
0.339AspCys: 0.339 ± 0.331
3.054AspAsp: 3.054 ± 0.58
3.903AspGlu: 3.903 ± 0.96
2.715AspPhe: 2.715 ± 0.597
4.412AspGly: 4.412 ± 0.867
1.188AspHis: 1.188 ± 0.715
4.073AspIle: 4.073 ± 0.732
3.903AspLys: 3.903 ± 1.116
3.224AspLeu: 3.224 ± 0.722
1.527AspMet: 1.527 ± 0.546
3.564AspAsn: 3.564 ± 0.867
4.751AspPro: 4.751 ± 1.048
1.697AspGln: 1.697 ± 0.495
2.206AspArg: 2.206 ± 0.612
2.885AspSer: 2.885 ± 0.866
4.582AspThr: 4.582 ± 0.597
3.394AspVal: 3.394 ± 1.111
1.018AspTrp: 1.018 ± 0.343
1.697AspTyr: 1.697 ± 0.384
0.0AspXaa: 0.0 ± 0.0
Glu
2.715GluAla: 2.715 ± 0.852
0.509GluCys: 0.509 ± 0.386
2.715GluAsp: 2.715 ± 0.793
2.036GluGlu: 2.036 ± 0.504
3.054GluPhe: 3.054 ± 0.828
3.054GluGly: 3.054 ± 0.53
0.339GluHis: 0.339 ± 0.208
3.733GluIle: 3.733 ± 0.778
1.867GluLys: 1.867 ± 0.54
5.939GluLeu: 5.939 ± 0.968
1.527GluMet: 1.527 ± 0.425
2.885GluAsn: 2.885 ± 0.783
2.376GluPro: 2.376 ± 0.634
2.715GluGln: 2.715 ± 0.703
4.073GluArg: 4.073 ± 0.996
1.867GluSer: 1.867 ± 0.659
4.242GluThr: 4.242 ± 0.664
2.036GluVal: 2.036 ± 0.613
1.188GluTrp: 1.188 ± 0.37
3.054GluTyr: 3.054 ± 0.712
0.0GluXaa: 0.0 ± 0.0
Phe
3.394PheAla: 3.394 ± 0.787
0.0PheCys: 0.0 ± 0.0
2.545PheAsp: 2.545 ± 0.632
2.715PheGlu: 2.715 ± 0.767
1.188PhePhe: 1.188 ± 0.431
2.885PheGly: 2.885 ± 0.513
0.679PheHis: 0.679 ± 0.299
3.394PheIle: 3.394 ± 0.875
1.867PheLys: 1.867 ± 0.457
1.867PheLeu: 1.867 ± 0.534
1.867PheMet: 1.867 ± 0.71
2.715PheAsn: 2.715 ± 0.563
1.358PhePro: 1.358 ± 0.455
0.848PheGln: 0.848 ± 0.382
2.545PheArg: 2.545 ± 0.742
1.018PheSer: 1.018 ± 0.373
3.224PheThr: 3.224 ± 0.629
2.715PheVal: 2.715 ± 0.739
0.509PheTrp: 0.509 ± 0.266
0.848PheTyr: 0.848 ± 0.428
0.0PheXaa: 0.0 ± 0.0
Gly
7.466GlyAla: 7.466 ± 1.149
0.17GlyCys: 0.17 ± 0.183
6.279GlyAsp: 6.279 ± 1.72
5.091GlyGlu: 5.091 ± 0.867
2.885GlyPhe: 2.885 ± 0.798
5.939GlyGly: 5.939 ± 1.166
1.867GlyHis: 1.867 ± 0.478
6.109GlyIle: 6.109 ± 1.216
4.242GlyLys: 4.242 ± 1.024
4.242GlyLeu: 4.242 ± 0.946
0.848GlyMet: 0.848 ± 0.411
4.242GlyAsn: 4.242 ± 0.957
2.206GlyPro: 2.206 ± 0.824
2.206GlyGln: 2.206 ± 0.514
4.582GlyArg: 4.582 ± 0.936
4.073GlySer: 4.073 ± 0.924
8.654GlyThr: 8.654 ± 1.509
6.279GlyVal: 6.279 ± 1.162
0.848GlyTrp: 0.848 ± 0.332
3.054GlyTyr: 3.054 ± 0.927
0.0GlyXaa: 0.0 ± 0.0
His
1.018HisAla: 1.018 ± 0.471
0.17HisCys: 0.17 ± 0.181
1.018HisAsp: 1.018 ± 0.419
0.848HisGlu: 0.848 ± 0.297
1.697HisPhe: 1.697 ± 0.509
1.188HisGly: 1.188 ± 0.33
0.339HisHis: 0.339 ± 0.279
0.848HisIle: 0.848 ± 0.431
0.679HisLys: 0.679 ± 0.31
1.527HisLeu: 1.527 ± 0.514
0.339HisMet: 0.339 ± 0.221
0.509HisAsn: 0.509 ± 0.252
0.848HisPro: 0.848 ± 0.377
0.679HisGln: 0.679 ± 0.474
2.206HisArg: 2.206 ± 0.511
1.018HisSer: 1.018 ± 0.464
0.848HisThr: 0.848 ± 0.35
1.188HisVal: 1.188 ± 0.531
0.339HisTrp: 0.339 ± 0.269
1.188HisTyr: 1.188 ± 0.563
0.0HisXaa: 0.0 ± 0.0
Ile
5.43IleAla: 5.43 ± 0.846
0.339IleCys: 0.339 ± 0.221
3.903IleAsp: 3.903 ± 0.569
3.224IleGlu: 3.224 ± 0.624
1.358IlePhe: 1.358 ± 0.484
5.26IleGly: 5.26 ± 0.798
0.679IleHis: 0.679 ± 0.282
2.545IleIle: 2.545 ± 0.633
2.885IleLys: 2.885 ± 0.81
2.885IleLeu: 2.885 ± 0.638
2.036IleMet: 2.036 ± 0.524
3.903IleAsn: 3.903 ± 0.927
3.054IlePro: 3.054 ± 0.676
2.545IleGln: 2.545 ± 0.465
2.376IleArg: 2.376 ± 0.956
2.885IleSer: 2.885 ± 0.585
3.903IleThr: 3.903 ± 0.875
3.224IleVal: 3.224 ± 0.723
0.679IleTrp: 0.679 ± 0.352
0.848IleTyr: 0.848 ± 0.372
0.0IleXaa: 0.0 ± 0.0
Lys
2.885LysAla: 2.885 ± 0.835
0.339LysCys: 0.339 ± 0.366
2.715LysAsp: 2.715 ± 0.596
3.224LysGlu: 3.224 ± 0.615
1.527LysPhe: 1.527 ± 0.506
3.733LysGly: 3.733 ± 1.717
1.018LysHis: 1.018 ± 0.422
1.358LysIle: 1.358 ± 0.366
2.036LysLys: 2.036 ± 0.918
4.921LysLeu: 4.921 ± 1.179
1.697LysMet: 1.697 ± 0.442
1.697LysAsn: 1.697 ± 0.538
2.545LysPro: 2.545 ± 0.592
2.715LysGln: 2.715 ± 0.708
3.054LysArg: 3.054 ± 0.647
1.188LysSer: 1.188 ± 0.613
3.054LysThr: 3.054 ± 0.879
3.394LysVal: 3.394 ± 0.592
0.509LysTrp: 0.509 ± 0.23
0.509LysTyr: 0.509 ± 0.243
0.0LysXaa: 0.0 ± 0.0
Leu
7.127LeuAla: 7.127 ± 0.987
0.17LeuCys: 0.17 ± 0.175
4.921LeuAsp: 4.921 ± 0.908
3.224LeuGlu: 3.224 ± 0.628
4.073LeuPhe: 4.073 ± 1.006
3.733LeuGly: 3.733 ± 0.792
1.018LeuHis: 1.018 ± 0.437
2.206LeuIle: 2.206 ± 0.579
4.751LeuLys: 4.751 ± 0.875
3.564LeuLeu: 3.564 ± 0.705
2.885LeuMet: 2.885 ± 0.74
3.733LeuAsn: 3.733 ± 0.884
4.412LeuPro: 4.412 ± 0.863
3.733LeuGln: 3.733 ± 0.662
3.054LeuArg: 3.054 ± 0.525
4.582LeuSer: 4.582 ± 0.761
6.109LeuThr: 6.109 ± 1.014
4.582LeuVal: 4.582 ± 0.545
1.697LeuTrp: 1.697 ± 0.566
1.527LeuTyr: 1.527 ± 0.494
0.0LeuXaa: 0.0 ± 0.0
Met
3.224MetAla: 3.224 ± 0.591
0.17MetCys: 0.17 ± 0.164
0.509MetAsp: 0.509 ± 0.355
0.848MetGlu: 0.848 ± 0.389
1.358MetPhe: 1.358 ± 0.755
1.358MetGly: 1.358 ± 0.381
0.339MetHis: 0.339 ± 0.263
0.679MetIle: 0.679 ± 0.399
0.679MetLys: 0.679 ± 0.397
2.206MetLeu: 2.206 ± 0.556
0.509MetMet: 0.509 ± 0.319
1.358MetAsn: 1.358 ± 0.451
1.527MetPro: 1.527 ± 0.459
0.509MetGln: 0.509 ± 0.28
1.697MetArg: 1.697 ± 0.651
1.867MetSer: 1.867 ± 0.443
2.036MetThr: 2.036 ± 0.543
1.527MetVal: 1.527 ± 0.556
0.17MetTrp: 0.17 ± 0.175
0.679MetTyr: 0.679 ± 0.27
0.0MetXaa: 0.0 ± 0.0
Asn
6.957AsnAla: 6.957 ± 0.818
0.339AsnCys: 0.339 ± 0.218
2.715AsnAsp: 2.715 ± 0.581
1.527AsnGlu: 1.527 ± 0.688
1.527AsnPhe: 1.527 ± 0.44
5.43AsnGly: 5.43 ± 1.002
0.679AsnHis: 0.679 ± 0.318
3.394AsnIle: 3.394 ± 0.766
1.188AsnLys: 1.188 ± 0.455
3.733AsnLeu: 3.733 ± 0.98
0.339AsnMet: 0.339 ± 0.232
3.903AsnAsn: 3.903 ± 0.687
3.054AsnPro: 3.054 ± 0.762
2.206AsnGln: 2.206 ± 0.56
2.545AsnArg: 2.545 ± 0.683
2.545AsnSer: 2.545 ± 0.571
3.564AsnThr: 3.564 ± 0.758
3.224AsnVal: 3.224 ± 0.626
1.697AsnTrp: 1.697 ± 0.563
2.885AsnTyr: 2.885 ± 0.865
0.0AsnXaa: 0.0 ± 0.0
Pro
4.751ProAla: 4.751 ± 0.858
0.509ProCys: 0.509 ± 0.241
3.733ProAsp: 3.733 ± 1.041
4.582ProGlu: 4.582 ± 0.838
0.339ProPhe: 0.339 ± 0.199
6.279ProGly: 6.279 ± 1.495
1.018ProHis: 1.018 ± 0.484
2.545ProIle: 2.545 ± 0.587
2.376ProLys: 2.376 ± 0.565
3.564ProLeu: 3.564 ± 0.664
0.339ProMet: 0.339 ± 0.255
2.376ProAsn: 2.376 ± 0.874
2.376ProPro: 2.376 ± 0.963
0.848ProGln: 0.848 ± 0.406
1.527ProArg: 1.527 ± 0.428
3.394ProSer: 3.394 ± 0.875
3.394ProThr: 3.394 ± 0.622
3.564ProVal: 3.564 ± 0.766
0.848ProTrp: 0.848 ± 0.342
1.697ProTyr: 1.697 ± 0.468
0.0ProXaa: 0.0 ± 0.0
Gln
5.26GlnAla: 5.26 ± 0.81
0.17GlnCys: 0.17 ± 0.171
2.376GlnAsp: 2.376 ± 0.706
2.036GlnGlu: 2.036 ± 0.503
2.376GlnPhe: 2.376 ± 0.606
3.054GlnGly: 3.054 ± 0.567
0.679GlnHis: 0.679 ± 0.359
2.036GlnIle: 2.036 ± 0.733
0.679GlnLys: 0.679 ± 0.384
3.564GlnLeu: 3.564 ± 0.813
0.0GlnMet: 0.0 ± 0.0
1.697GlnAsn: 1.697 ± 0.473
2.036GlnPro: 2.036 ± 0.585
2.545GlnGln: 2.545 ± 1.026
1.527GlnArg: 1.527 ± 0.434
1.697GlnSer: 1.697 ± 0.576
3.394GlnThr: 3.394 ± 0.609
2.376GlnVal: 2.376 ± 0.487
0.679GlnTrp: 0.679 ± 0.356
1.018GlnTyr: 1.018 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
5.26ArgAla: 5.26 ± 1.117
0.339ArgCys: 0.339 ± 0.35
2.885ArgAsp: 2.885 ± 0.744
3.054ArgGlu: 3.054 ± 0.827
2.715ArgPhe: 2.715 ± 0.841
3.903ArgGly: 3.903 ± 0.978
0.848ArgHis: 0.848 ± 0.314
3.054ArgIle: 3.054 ± 0.744
2.036ArgLys: 2.036 ± 0.567
3.564ArgLeu: 3.564 ± 0.753
1.527ArgMet: 1.527 ± 0.642
2.885ArgAsn: 2.885 ± 0.755
2.545ArgPro: 2.545 ± 0.59
3.054ArgGln: 3.054 ± 1.006
2.545ArgArg: 2.545 ± 0.785
2.036ArgSer: 2.036 ± 0.525
2.545ArgThr: 2.545 ± 0.599
2.545ArgVal: 2.545 ± 0.799
1.018ArgTrp: 1.018 ± 0.557
2.036ArgTyr: 2.036 ± 0.613
0.0ArgXaa: 0.0 ± 0.0
Ser
6.788SerAla: 6.788 ± 1.672
1.188SerCys: 1.188 ± 0.625
3.733SerAsp: 3.733 ± 0.666
3.224SerGlu: 3.224 ± 1.01
1.018SerPhe: 1.018 ± 0.332
3.394SerGly: 3.394 ± 0.899
0.848SerHis: 0.848 ± 0.35
3.054SerIle: 3.054 ± 0.571
0.848SerLys: 0.848 ± 0.405
4.412SerLeu: 4.412 ± 0.921
1.527SerMet: 1.527 ± 0.441
1.527SerAsn: 1.527 ± 0.538
2.036SerPro: 2.036 ± 0.782
2.036SerGln: 2.036 ± 0.612
2.545SerArg: 2.545 ± 0.884
2.885SerSer: 2.885 ± 1.082
4.073SerThr: 4.073 ± 0.908
4.412SerVal: 4.412 ± 0.988
0.848SerTrp: 0.848 ± 0.343
1.358SerTyr: 1.358 ± 0.405
0.0SerXaa: 0.0 ± 0.0
Thr
8.994ThrAla: 8.994 ± 1.45
0.509ThrCys: 0.509 ± 0.321
3.394ThrAsp: 3.394 ± 0.459
3.224ThrGlu: 3.224 ± 0.743
3.394ThrPhe: 3.394 ± 0.863
7.806ThrGly: 7.806 ± 1.464
2.376ThrHis: 2.376 ± 0.798
4.751ThrIle: 4.751 ± 0.626
2.885ThrLys: 2.885 ± 0.722
5.26ThrLeu: 5.26 ± 0.835
1.358ThrMet: 1.358 ± 0.406
3.394ThrAsn: 3.394 ± 0.632
4.073ThrPro: 4.073 ± 0.967
2.376ThrGln: 2.376 ± 0.459
3.054ThrArg: 3.054 ± 0.809
3.733ThrSer: 3.733 ± 0.933
7.466ThrThr: 7.466 ± 1.43
7.806ThrVal: 7.806 ± 1.712
0.848ThrTrp: 0.848 ± 0.389
4.921ThrTyr: 4.921 ± 0.731
0.0ThrXaa: 0.0 ± 0.0
Val
6.957ValAla: 6.957 ± 1.144
0.679ValCys: 0.679 ± 0.479
4.751ValAsp: 4.751 ± 1.029
2.376ValGlu: 2.376 ± 0.707
2.545ValPhe: 2.545 ± 0.712
5.091ValGly: 5.091 ± 0.757
1.358ValHis: 1.358 ± 0.429
2.036ValIle: 2.036 ± 0.446
2.376ValLys: 2.376 ± 0.516
5.6ValLeu: 5.6 ± 1.095
0.848ValMet: 0.848 ± 0.344
3.564ValAsn: 3.564 ± 0.72
3.564ValPro: 3.564 ± 0.799
2.545ValGln: 2.545 ± 0.595
2.545ValArg: 2.545 ± 0.694
5.091ValSer: 5.091 ± 0.58
7.976ValThr: 7.976 ± 2.112
4.242ValVal: 4.242 ± 0.896
1.018ValTrp: 1.018 ± 0.444
1.527ValTyr: 1.527 ± 0.424
0.0ValXaa: 0.0 ± 0.0
Trp
2.376TrpAla: 2.376 ± 0.666
0.17TrpCys: 0.17 ± 0.166
1.527TrpAsp: 1.527 ± 0.418
0.509TrpGlu: 0.509 ± 0.306
0.0TrpPhe: 0.0 ± 0.0
1.358TrpGly: 1.358 ± 0.501
0.339TrpHis: 0.339 ± 0.269
1.018TrpIle: 1.018 ± 0.366
1.527TrpLys: 1.527 ± 0.522
1.697TrpLeu: 1.697 ± 0.602
0.509TrpMet: 0.509 ± 0.29
1.018TrpAsn: 1.018 ± 0.4
0.848TrpPro: 0.848 ± 0.332
0.679TrpGln: 0.679 ± 0.269
0.17TrpArg: 0.17 ± 0.139
0.509TrpSer: 0.509 ± 0.255
0.848TrpThr: 0.848 ± 0.376
0.509TrpVal: 0.509 ± 0.268
0.17TrpTrp: 0.17 ± 0.175
0.339TrpTyr: 0.339 ± 0.251
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.921TyrAla: 4.921 ± 1.101
0.0TyrCys: 0.0 ± 0.0
2.376TyrAsp: 2.376 ± 0.741
1.018TyrGlu: 1.018 ± 0.359
0.848TyrPhe: 0.848 ± 0.383
3.054TyrGly: 3.054 ± 0.965
0.848TyrHis: 0.848 ± 0.37
1.697TyrIle: 1.697 ± 0.465
1.358TyrLys: 1.358 ± 0.413
2.036TyrLeu: 2.036 ± 0.556
0.848TyrMet: 0.848 ± 0.428
1.867TyrAsn: 1.867 ± 0.585
1.358TyrPro: 1.358 ± 0.482
1.527TyrGln: 1.527 ± 0.438
2.206TyrArg: 2.206 ± 0.489
2.885TyrSer: 2.885 ± 0.703
2.715TyrThr: 2.715 ± 0.531
1.867TyrVal: 1.867 ± 0.553
0.848TyrTrp: 0.848 ± 0.31
1.527TyrTyr: 1.527 ± 0.439
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 27 proteins (5894 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski