Haloarcula sp. JP-L23

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Haloarcula; unclassified Haloarcula

Average proteome isoelectric point is 4.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4757 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8TAZ4|A0A6G8TAZ4_9EURY Uncharacterized protein OS=Haloarcula sp. JP-L23 OX=2716717 GN=G9465_17025 PE=4 SV=1
MM1 pKa = 7.66ISGTGVRR8 pKa = 11.84SLLLQTGIGPIDD20 pKa = 4.5AIIGLFVSIVLGIVNFAAQVLPTVLLALLVLGVGYY55 pKa = 10.38VVADD59 pKa = 4.09RR60 pKa = 11.84LDD62 pKa = 3.5TFVFEE67 pKa = 4.45WALEE71 pKa = 3.85NDD73 pKa = 3.79VDD75 pKa = 4.6GKK77 pKa = 11.06AGDD80 pKa = 4.17TPASTVVADD89 pKa = 3.98EE90 pKa = 5.27DD91 pKa = 4.53GVATAAGQLVRR102 pKa = 11.84YY103 pKa = 8.64LVLVVAVVAAIRR115 pKa = 11.84VLNLSEE121 pKa = 4.23LQEE124 pKa = 4.36LLDD127 pKa = 3.66IVIGYY132 pKa = 8.42VPNVVAAAVLLVVGFGVGRR151 pKa = 11.84IVRR154 pKa = 11.84SLVPGLVDD162 pKa = 3.45RR163 pKa = 11.84TDD165 pKa = 4.09LGDD168 pKa = 3.94SFPTTHH174 pKa = 7.4FGRR177 pKa = 11.84VVDD180 pKa = 4.21ADD182 pKa = 3.58GGTVGRR188 pKa = 11.84LAGIAVEE195 pKa = 4.28YY196 pKa = 10.34YY197 pKa = 9.95VYY199 pKa = 10.96LVTLYY204 pKa = 10.69VVAATLAIGSVAGVLGAAVSYY225 pKa = 11.21VPTLLGALVLLLLGAIVADD244 pKa = 3.78YY245 pKa = 10.82VGTVVRR251 pKa = 11.84GVDD254 pKa = 3.88EE255 pKa = 4.52VADD258 pKa = 4.02SPFEE262 pKa = 4.06SLVTGGVQAVVYY274 pKa = 9.74LFTVVIALDD283 pKa = 3.67VAGVDD288 pKa = 3.53ATLLGLLLLAVVLPVGLAFALAFGLSVGYY317 pKa = 10.36GGQDD321 pKa = 3.07YY322 pKa = 10.96VEE324 pKa = 4.6QYY326 pKa = 11.17LGTDD330 pKa = 3.47EE331 pKa = 4.86AAEE334 pKa = 4.03

Molecular weight: 34.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8TF09|A0A6G8TF09_9EURY Uncharacterized protein OS=Haloarcula sp. JP-L23 OX=2716717 GN=G9465_24110 PE=4 SV=1
MM1 pKa = 7.26SRR3 pKa = 11.84KK4 pKa = 9.88LYY6 pKa = 10.15FVEE9 pKa = 4.23LAEE12 pKa = 4.26TEE14 pKa = 4.24RR15 pKa = 11.84AEE17 pKa = 4.9LEE19 pKa = 4.21ALVSAGNHH27 pKa = 5.26SAQKK31 pKa = 8.92LTRR34 pKa = 11.84ARR36 pKa = 11.84ILLKK40 pKa = 10.33SDD42 pKa = 4.26EE43 pKa = 4.41GWSDD47 pKa = 3.4PKK49 pKa = 10.73IADD52 pKa = 3.8ALDD55 pKa = 3.73CGRR58 pKa = 11.84ATVQRR63 pKa = 11.84TRR65 pKa = 11.84EE66 pKa = 3.79RR67 pKa = 11.84FSRR70 pKa = 11.84QRR72 pKa = 11.84LGAIEE77 pKa = 4.31RR78 pKa = 11.84KK79 pKa = 9.81NPAVSPRR86 pKa = 11.84ANSTATPRR94 pKa = 11.84PIDD97 pKa = 3.47CVGVQRR103 pKa = 11.84AARR106 pKa = 11.84RR107 pKa = 11.84ARR109 pKa = 11.84SLDD112 pKa = 3.1ASPPRR117 pKa = 11.84RR118 pKa = 11.84HH119 pKa = 6.28ACHH122 pKa = 6.16PQQ124 pKa = 2.76

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4757

0

4757

1338033

36

2113

281.3

30.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.633 ± 0.045

0.75 ± 0.011

8.459 ± 0.053

8.105 ± 0.056

3.298 ± 0.024

8.343 ± 0.041

2.051 ± 0.018

3.874 ± 0.026

1.868 ± 0.022

8.878 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.821 ± 0.015

2.42 ± 0.023

4.663 ± 0.025

2.795 ± 0.022

6.331 ± 0.035

5.622 ± 0.032

6.85 ± 0.041

9.279 ± 0.042

1.186 ± 0.016

2.774 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski