Amino acid dipepetide frequency for Haloarcula sp. JP-L23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.788AlaAla: 12.788 ± 0.15
0.732AlaCys: 0.732 ± 0.03
9.675AlaAsp: 9.675 ± 0.1
8.042AlaGlu: 8.042 ± 0.1
3.809AlaPhe: 3.809 ± 0.057
8.878AlaGly: 8.878 ± 0.093
1.85AlaHis: 1.85 ± 0.035
4.664AlaIle: 4.664 ± 0.072
1.936AlaLys: 1.936 ± 0.04
9.885AlaLeu: 9.885 ± 0.1
2.185AlaMet: 2.185 ± 0.046
2.455AlaAsn: 2.455 ± 0.045
3.804AlaPro: 3.804 ± 0.057
2.398AlaGln: 2.398 ± 0.042
5.814AlaArg: 5.814 ± 0.071
5.472AlaSer: 5.472 ± 0.071
7.409AlaThr: 7.409 ± 0.089
10.666AlaVal: 10.666 ± 0.123
1.131AlaTrp: 1.131 ± 0.03
2.735AlaTyr: 2.735 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.588CysAla: 0.588 ± 0.023
0.086CysCys: 0.086 ± 0.008
0.579CysAsp: 0.579 ± 0.02
0.619CysGlu: 0.619 ± 0.023
0.197CysPhe: 0.197 ± 0.014
0.887CysGly: 0.887 ± 0.028
0.206CysHis: 0.206 ± 0.014
0.289CysIle: 0.289 ± 0.015
0.129CysLys: 0.129 ± 0.01
0.586CysLeu: 0.586 ± 0.022
0.122CysMet: 0.122 ± 0.009
0.194CysAsn: 0.194 ± 0.012
0.565CysPro: 0.565 ± 0.022
0.207CysGln: 0.207 ± 0.013
0.49CysArg: 0.49 ± 0.021
0.455CysSer: 0.455 ± 0.02
0.437CysThr: 0.437 ± 0.02
0.523CysVal: 0.523 ± 0.021
0.115CysTrp: 0.115 ± 0.009
0.228CysTyr: 0.228 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
9.72AspAla: 9.72 ± 0.111
0.699AspCys: 0.699 ± 0.027
7.731AspAsp: 7.731 ± 0.1
7.629AspGlu: 7.629 ± 0.104
2.026AspPhe: 2.026 ± 0.042
8.255AspGly: 8.255 ± 0.102
2.055AspHis: 2.055 ± 0.04
3.471AspIle: 3.471 ± 0.06
0.993AspLys: 0.993 ± 0.028
6.655AspLeu: 6.655 ± 0.082
1.303AspMet: 1.303 ± 0.033
1.41AspAsn: 1.41 ± 0.035
4.76AspPro: 4.76 ± 0.08
1.788AspGln: 1.788 ± 0.039
6.167AspArg: 6.167 ± 0.073
4.047AspSer: 4.047 ± 0.067
4.59AspThr: 4.59 ± 0.071
8.176AspVal: 8.176 ± 0.087
1.058AspTrp: 1.058 ± 0.026
2.053AspTyr: 2.053 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
8.166GluAla: 8.166 ± 0.093
0.581GluCys: 0.581 ± 0.022
5.748GluAsp: 5.748 ± 0.082
6.056GluGlu: 6.056 ± 0.095
2.788GluPhe: 2.788 ± 0.043
5.113GluGly: 5.113 ± 0.069
1.801GluHis: 1.801 ± 0.039
3.283GluIle: 3.283 ± 0.06
1.867GluLys: 1.867 ± 0.045
6.919GluLeu: 6.919 ± 0.091
1.995GluMet: 1.995 ± 0.033
2.389GluAsn: 2.389 ± 0.043
3.158GluPro: 3.158 ± 0.053
3.022GluGln: 3.022 ± 0.065
7.057GluArg: 7.057 ± 0.093
5.038GluSer: 5.038 ± 0.065
6.829GluThr: 6.829 ± 0.085
5.409GluVal: 5.409 ± 0.067
1.16GluTrp: 1.16 ± 0.032
2.67GluTyr: 2.67 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
3.477PheAla: 3.477 ± 0.061
0.291PheCys: 0.291 ± 0.015
3.224PheAsp: 3.224 ± 0.048
3.164PheGlu: 3.164 ± 0.054
1.016PhePhe: 1.016 ± 0.032
3.226PheGly: 3.226 ± 0.05
0.682PheHis: 0.682 ± 0.025
1.075PheIle: 1.075 ± 0.036
0.489PheLys: 0.489 ± 0.021
2.815PheLeu: 2.815 ± 0.05
0.507PheMet: 0.507 ± 0.019
0.738PheAsn: 0.738 ± 0.022
1.343PhePro: 1.343 ± 0.034
0.883PheGln: 0.883 ± 0.024
1.756PheArg: 1.756 ± 0.038
1.739PheSer: 1.739 ± 0.042
1.962PheThr: 1.962 ± 0.04
3.285PheVal: 3.285 ± 0.055
0.41PheTrp: 0.41 ± 0.019
0.898PheTyr: 0.898 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
7.606GlyAla: 7.606 ± 0.095
0.69GlyCys: 0.69 ± 0.025
6.585GlyAsp: 6.585 ± 0.082
6.637GlyGlu: 6.637 ± 0.073
2.997GlyPhe: 2.997 ± 0.044
7.388GlyGly: 7.388 ± 0.106
1.708GlyHis: 1.708 ± 0.038
4.01GlyIle: 4.01 ± 0.064
1.831GlyLys: 1.831 ± 0.043
7.457GlyLeu: 7.457 ± 0.093
1.663GlyMet: 1.663 ± 0.036
1.995GlyAsn: 1.995 ± 0.037
3.544GlyPro: 3.544 ± 0.052
2.326GlyGln: 2.326 ± 0.044
4.709GlyArg: 4.709 ± 0.061
4.998GlySer: 4.998 ± 0.067
5.946GlyThr: 5.946 ± 0.074
7.594GlyVal: 7.594 ± 0.085
1.092GlyTrp: 1.092 ± 0.031
2.655GlyTyr: 2.655 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
1.906HisAla: 1.906 ± 0.039
0.226HisCys: 0.226 ± 0.013
1.898HisAsp: 1.898 ± 0.041
1.708HisGlu: 1.708 ± 0.036
0.587HisPhe: 0.587 ± 0.022
1.921HisGly: 1.921 ± 0.04
0.564HisHis: 0.564 ± 0.023
0.719HisIle: 0.719 ± 0.022
0.347HisLys: 0.347 ± 0.017
1.815HisLeu: 1.815 ± 0.04
0.318HisMet: 0.318 ± 0.015
0.5HisAsn: 0.5 ± 0.019
1.223HisPro: 1.223 ± 0.03
0.515HisGln: 0.515 ± 0.02
1.302HisArg: 1.302 ± 0.028
0.96HisSer: 0.96 ± 0.027
1.174HisThr: 1.174 ± 0.028
1.951HisVal: 1.951 ± 0.04
0.271HisTrp: 0.271 ± 0.017
0.609HisTyr: 0.609 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
4.531IleAla: 4.531 ± 0.066
0.275IleCys: 0.275 ± 0.016
3.66IleAsp: 3.66 ± 0.064
3.868IleGlu: 3.868 ± 0.062
0.951IlePhe: 0.951 ± 0.035
3.46IleGly: 3.46 ± 0.062
0.789IleHis: 0.789 ± 0.028
1.288IleIle: 1.288 ± 0.04
0.741IleLys: 0.741 ± 0.025
2.912IleLeu: 2.912 ± 0.056
0.488IleMet: 0.488 ± 0.02
0.971IleAsn: 0.971 ± 0.025
2.06IlePro: 2.06 ± 0.044
1.141IleGln: 1.141 ± 0.03
2.39IleArg: 2.39 ± 0.043
2.113IleSer: 2.113 ± 0.044
2.356IleThr: 2.356 ± 0.045
3.486IleVal: 3.486 ± 0.057
0.33IleTrp: 0.33 ± 0.015
0.925IleTyr: 0.925 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
1.804LysAla: 1.804 ± 0.038
0.135LysCys: 0.135 ± 0.01
1.116LysAsp: 1.116 ± 0.028
1.337LysGlu: 1.337 ± 0.039
0.549LysPhe: 0.549 ± 0.02
1.321LysGly: 1.321 ± 0.03
0.499LysHis: 0.499 ± 0.019
0.78LysIle: 0.78 ± 0.028
0.525LysLys: 0.525 ± 0.021
1.712LysLeu: 1.712 ± 0.039
0.382LysMet: 0.382 ± 0.017
0.558LysAsn: 0.558 ± 0.021
0.917LysPro: 0.917 ± 0.026
0.916LysGln: 0.916 ± 0.026
1.624LysArg: 1.624 ± 0.039
1.137LysSer: 1.137 ± 0.031
1.354LysThr: 1.354 ± 0.034
1.214LysVal: 1.214 ± 0.033
0.253LysTrp: 0.253 ± 0.013
0.548LysTyr: 0.548 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
10.657LeuAla: 10.657 ± 0.115
0.654LeuCys: 0.654 ± 0.023
7.592LeuAsp: 7.592 ± 0.092
6.183LeuGlu: 6.183 ± 0.084
3.104LeuPhe: 3.104 ± 0.06
7.477LeuGly: 7.477 ± 0.08
1.54LeuHis: 1.54 ± 0.037
2.591LeuIle: 2.591 ± 0.054
1.548LeuLys: 1.548 ± 0.039
8.711LeuLeu: 8.711 ± 0.12
1.336LeuMet: 1.336 ± 0.032
1.904LeuAsn: 1.904 ± 0.043
4.202LeuPro: 4.202 ± 0.064
2.391LeuGln: 2.391 ± 0.048
5.357LeuArg: 5.357 ± 0.066
5.774LeuSer: 5.774 ± 0.071
5.85LeuThr: 5.85 ± 0.06
8.66LeuVal: 8.66 ± 0.091
0.936LeuTrp: 0.936 ± 0.027
2.318LeuTyr: 2.318 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
1.968MetAla: 1.968 ± 0.044
0.129MetCys: 0.129 ± 0.01
1.371MetAsp: 1.371 ± 0.031
1.123MetGlu: 1.123 ± 0.028
0.535MetPhe: 0.535 ± 0.021
1.492MetGly: 1.492 ± 0.038
0.378MetHis: 0.378 ± 0.019
0.584MetIle: 0.584 ± 0.021
0.408MetLys: 0.408 ± 0.017
1.582MetLeu: 1.582 ± 0.039
0.333MetMet: 0.333 ± 0.016
0.555MetAsn: 0.555 ± 0.02
0.874MetPro: 0.874 ± 0.026
0.554MetGln: 0.554 ± 0.02
1.111MetArg: 1.111 ± 0.032
1.561MetSer: 1.561 ± 0.034
1.611MetThr: 1.611 ± 0.036
1.456MetVal: 1.456 ± 0.03
0.163MetTrp: 0.163 ± 0.009
0.425MetTyr: 0.425 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.504AsnAla: 2.504 ± 0.05
0.235AsnCys: 0.235 ± 0.014
1.764AsnAsp: 1.764 ± 0.038
1.801AsnGlu: 1.801 ± 0.038
0.729AsnPhe: 0.729 ± 0.025
2.143AsnGly: 2.143 ± 0.042
0.581AsnHis: 0.581 ± 0.019
0.992AsnIle: 0.992 ± 0.026
0.468AsnLys: 0.468 ± 0.023
2.009AsnLeu: 2.009 ± 0.036
0.451AsnMet: 0.451 ± 0.015
0.617AsnAsn: 0.617 ± 0.025
1.616AsnPro: 1.616 ± 0.035
0.732AsnGln: 0.732 ± 0.027
1.587AsnArg: 1.587 ± 0.033
1.129AsnSer: 1.129 ± 0.032
1.484AsnThr: 1.484 ± 0.035
2.299AsnVal: 2.299 ± 0.044
0.346AsnTrp: 0.346 ± 0.018
0.714AsnTyr: 0.714 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
4.543ProAla: 4.543 ± 0.065
0.252ProCys: 0.252 ± 0.014
4.912ProAsp: 4.912 ± 0.066
4.443ProGlu: 4.443 ± 0.063
1.614ProPhe: 1.614 ± 0.036
3.622ProGly: 3.622 ± 0.052
0.935ProHis: 0.935 ± 0.023
1.761ProIle: 1.761 ± 0.04
0.886ProLys: 0.886 ± 0.027
3.808ProLeu: 3.808 ± 0.067
0.88ProMet: 0.88 ± 0.026
1.228ProAsn: 1.228 ± 0.032
2.247ProPro: 2.247 ± 0.043
1.112ProGln: 1.112 ± 0.032
2.29ProArg: 2.29 ± 0.046
2.759ProSer: 2.759 ± 0.047
3.355ProThr: 3.355 ± 0.056
4.288ProVal: 4.288 ± 0.058
0.524ProTrp: 0.524 ± 0.019
1.173ProTyr: 1.173 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
2.666GlnAla: 2.666 ± 0.053
0.214GlnCys: 0.214 ± 0.014
1.472GlnAsp: 1.472 ± 0.035
1.749GlnGlu: 1.749 ± 0.041
1.226GlnPhe: 1.226 ± 0.032
1.762GlnGly: 1.762 ± 0.037
0.617GlnHis: 0.617 ± 0.022
1.15GlnIle: 1.15 ± 0.033
0.66GlnLys: 0.66 ± 0.025
2.5GlnLeu: 2.5 ± 0.044
0.625GlnMet: 0.625 ± 0.019
0.89GlnAsn: 0.89 ± 0.027
1.186GlnPro: 1.186 ± 0.035
1.343GlnGln: 1.343 ± 0.039
2.266GlnArg: 2.266 ± 0.05
2.069GlnSer: 2.069 ± 0.044
2.185GlnThr: 2.185 ± 0.046
1.986GlnVal: 1.986 ± 0.04
0.412GlnTrp: 0.412 ± 0.017
0.968GlnTyr: 0.968 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
6.173ArgAla: 6.173 ± 0.074
0.474ArgCys: 0.474 ± 0.02
4.99ArgAsp: 4.99 ± 0.071
5.975ArgGlu: 5.975 ± 0.078
2.235ArgPhe: 2.235 ± 0.05
4.152ArgGly: 4.152 ± 0.059
1.249ArgHis: 1.249 ± 0.032
2.806ArgIle: 2.806 ± 0.05
1.389ArgLys: 1.389 ± 0.034
5.952ArgLeu: 5.952 ± 0.077
1.259ArgMet: 1.259 ± 0.029
1.62ArgAsn: 1.62 ± 0.036
2.738ArgPro: 2.738 ± 0.052
2.049ArgGln: 2.049 ± 0.038
4.744ArgArg: 4.744 ± 0.074
3.448ArgSer: 3.448 ± 0.049
4.074ArgThr: 4.074 ± 0.063
5.224ArgVal: 5.224 ± 0.072
0.821ArgTrp: 0.821 ± 0.025
1.942ArgTyr: 1.942 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.394SerAla: 5.394 ± 0.081
0.327SerCys: 0.327 ± 0.015
4.672SerAsp: 4.672 ± 0.063
4.506SerGlu: 4.506 ± 0.065
1.882SerPhe: 1.882 ± 0.039
5.423SerGly: 5.423 ± 0.067
1.043SerHis: 1.043 ± 0.028
2.338SerIle: 2.338 ± 0.046
1.254SerLys: 1.254 ± 0.033
5.243SerLeu: 5.243 ± 0.067
1.132SerMet: 1.132 ± 0.027
1.442SerAsn: 1.442 ± 0.04
2.702SerPro: 2.702 ± 0.047
1.506SerGln: 1.506 ± 0.035
2.985SerArg: 2.985 ± 0.048
3.112SerSer: 3.112 ± 0.067
3.774SerThr: 3.774 ± 0.063
5.385SerVal: 5.385 ± 0.072
0.623SerTrp: 0.623 ± 0.025
1.479SerTyr: 1.479 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
7.513ThrAla: 7.513 ± 0.093
0.389ThrCys: 0.389 ± 0.017
6.089ThrAsp: 6.089 ± 0.081
5.015ThrGlu: 5.015 ± 0.076
2.276ThrPhe: 2.276 ± 0.044
5.872ThrGly: 5.872 ± 0.079
1.324ThrHis: 1.324 ± 0.035
2.912ThrIle: 2.912 ± 0.047
1.087ThrLys: 1.087 ± 0.027
6.288ThrLeu: 6.288 ± 0.068
1.164ThrMet: 1.164 ± 0.028
1.576ThrAsn: 1.576 ± 0.036
3.496ThrPro: 3.496 ± 0.059
1.67ThrGln: 1.67 ± 0.039
3.386ThrArg: 3.386 ± 0.054
3.196ThrSer: 3.196 ± 0.057
4.767ThrThr: 4.767 ± 0.08
7.965ThrVal: 7.965 ± 0.093
0.69ThrTrp: 0.69 ± 0.027
1.829ThrTyr: 1.829 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
10.536ValAla: 10.536 ± 0.108
0.725ValCys: 0.725 ± 0.022
7.891ValAsp: 7.891 ± 0.078
7.91ValGlu: 7.91 ± 0.089
3.102ValPhe: 3.102 ± 0.054
7.908ValGly: 7.908 ± 0.107
1.694ValHis: 1.694 ± 0.037
2.831ValIle: 2.831 ± 0.05
1.421ValLys: 1.421 ± 0.034
8.116ValLeu: 8.116 ± 0.095
1.403ValMet: 1.403 ± 0.031
2.049ValAsn: 2.049 ± 0.041
4.413ValPro: 4.413 ± 0.064
2.178ValGln: 2.178 ± 0.039
5.421ValArg: 5.421 ± 0.068
5.294ValSer: 5.294 ± 0.066
6.849ValThr: 6.849 ± 0.098
9.819ValVal: 9.819 ± 0.129
0.939ValTrp: 0.939 ± 0.035
2.285ValTyr: 2.285 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
1.046TrpAla: 1.046 ± 0.032
0.091TrpCys: 0.091 ± 0.009
0.894TrpAsp: 0.894 ± 0.029
0.81TrpGlu: 0.81 ± 0.031
0.46TrpPhe: 0.46 ± 0.018
0.824TrpGly: 0.824 ± 0.026
0.284TrpHis: 0.284 ± 0.017
0.415TrpIle: 0.415 ± 0.019
0.277TrpLys: 0.277 ± 0.014
1.318TrpLeu: 1.318 ± 0.033
0.245TrpMet: 0.245 ± 0.013
0.399TrpAsn: 0.399 ± 0.018
0.485TrpPro: 0.485 ± 0.018
0.416TrpGln: 0.416 ± 0.019
0.867TrpArg: 0.867 ± 0.026
0.623TrpSer: 0.623 ± 0.025
0.847TrpThr: 0.847 ± 0.027
0.963TrpVal: 0.963 ± 0.027
0.226TrpTrp: 0.226 ± 0.015
0.368TrpTyr: 0.368 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.743TyrAla: 2.743 ± 0.052
0.299TyrCys: 0.299 ± 0.015
2.724TyrAsp: 2.724 ± 0.045
2.475TyrGlu: 2.475 ± 0.044
0.896TyrPhe: 0.896 ± 0.026
2.309TyrGly: 2.309 ± 0.051
0.714TyrHis: 0.714 ± 0.023
0.777TyrIle: 0.777 ± 0.023
0.425TyrLys: 0.425 ± 0.02
2.5TyrLeu: 2.5 ± 0.045
0.422TyrMet: 0.422 ± 0.02
0.709TyrAsn: 0.709 ± 0.024
1.297TyrPro: 1.297 ± 0.032
0.8TyrGln: 0.8 ± 0.025
1.991TyrArg: 1.991 ± 0.041
1.295TyrSer: 1.295 ± 0.029
1.651TyrThr: 1.651 ± 0.036
2.434TyrVal: 2.434 ± 0.046
0.361TyrTrp: 0.361 ± 0.018
0.915TyrTyr: 0.915 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4757 proteins (1338033 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski