Bilophila wadsworthia (strain 3_1_6)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Bilophila; Bilophila wadsworthia

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5Y4H5|E5Y4H5_BILW3 Uncharacterized protein OS=Bilophila wadsworthia (strain 3_1_6) OX=563192 GN=HMPREF0179_01087 PE=4 SV=1
MM1 pKa = 6.8KK2 pKa = 9.72TVVVKK7 pKa = 10.37PEE9 pKa = 3.79FAGLTEE15 pKa = 4.11EE16 pKa = 4.36QVIDD20 pKa = 5.43AIDD23 pKa = 3.87AYY25 pKa = 9.74EE26 pKa = 4.22AQVDD30 pKa = 3.88EE31 pKa = 4.84YY32 pKa = 10.84YY33 pKa = 11.26ANKK36 pKa = 10.19GDD38 pKa = 4.65EE39 pKa = 4.38EE40 pKa = 4.6EE41 pKa = 4.69CGCGCSHH48 pKa = 8.08DD49 pKa = 4.36EE50 pKa = 4.66GSCEE54 pKa = 5.26DD55 pKa = 4.17SDD57 pKa = 5.92DD58 pKa = 4.84DD59 pKa = 5.73VIPSDD64 pKa = 4.05DD65 pKa = 4.65PIVLEE70 pKa = 4.09VEE72 pKa = 4.21RR73 pKa = 11.84LIAAYY78 pKa = 10.02SDD80 pKa = 4.43RR81 pKa = 11.84FDD83 pKa = 4.21EE84 pKa = 4.47LCKK87 pKa = 10.9EE88 pKa = 4.01NGEE91 pKa = 4.34VPEE94 pKa = 4.32EE95 pKa = 3.9ALTYY99 pKa = 10.34KK100 pKa = 10.27PRR102 pKa = 11.84TPIEE106 pKa = 3.79QVAFDD111 pKa = 4.09IFTDD115 pKa = 3.71ALHH118 pKa = 7.15DD119 pKa = 4.04SLMDD123 pKa = 4.12EE124 pKa = 5.04DD125 pKa = 5.79DD126 pKa = 3.72EE127 pKa = 4.76

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5Y2F7|E5Y2F7_BILW3 Uncharacterized protein OS=Bilophila wadsworthia (strain 3_1_6) OX=563192 GN=HMPREF0179_00368 PE=4 SV=1
MM1 pKa = 7.18PTVFSHH7 pKa = 6.88PAPVLALAALLGGRR21 pKa = 11.84LSTRR25 pKa = 11.84MLLFGILCAVLPDD38 pKa = 3.65ADD40 pKa = 3.78VIGFRR45 pKa = 11.84FGISYY50 pKa = 11.16ADD52 pKa = 3.42AFGHH56 pKa = 6.64RR57 pKa = 11.84GFSHH61 pKa = 6.5SLAFALLMGCAGFGVAPLFLRR82 pKa = 11.84GSRR85 pKa = 11.84LMGFTVGLLAVSSHH99 pKa = 6.19ILLDD103 pKa = 3.53AMTNGGLGVAAFWPFDD119 pKa = 3.36QTRR122 pKa = 11.84YY123 pKa = 9.09FCDD126 pKa = 2.83WRR128 pKa = 11.84PIRR131 pKa = 11.84VSPFGLKK138 pKa = 10.47GLLSQRR144 pKa = 11.84GLSVMLSEE152 pKa = 5.54LRR154 pKa = 11.84WVWAPCLAVIAAALFFGKK172 pKa = 10.16NPMRR176 pKa = 11.84AIPRR180 pKa = 11.84KK181 pKa = 9.64

Molecular weight: 19.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3779

0

3779

1220490

29

4198

323.0

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.286 ± 0.047

1.499 ± 0.018

5.184 ± 0.036

6.238 ± 0.038

4.025 ± 0.03

8.313 ± 0.044

2.022 ± 0.016

5.315 ± 0.032

4.279 ± 0.042

10.548 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.789 ± 0.024

3.027 ± 0.026

5.131 ± 0.032

3.082 ± 0.021

6.257 ± 0.046

5.761 ± 0.029

5.204 ± 0.058

7.104 ± 0.031

1.275 ± 0.017

2.661 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski