Streptomyces xiamenensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5490 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0F7FZY1|A0A0F7FZY1_9ACTN Membrane protein OS=Streptomyces xiamenensis OX=408015 GN=SXIM_50610 PE=4 SV=1
MM1 pKa = 7.76DD2 pKa = 4.04TMSNTTVRR10 pKa = 11.84RR11 pKa = 11.84LVRR14 pKa = 11.84TTAVVGALALGVTACGSSDD33 pKa = 3.92DD34 pKa = 4.16GDD36 pKa = 4.04GAGGGGGASSGLTPADD52 pKa = 3.12VEE54 pKa = 4.26AALEE58 pKa = 4.22EE59 pKa = 4.85GGSLTVWSWEE69 pKa = 3.85ATLPAAAQAFMDD81 pKa = 3.49EE82 pKa = 4.41HH83 pKa = 6.91PNVKK87 pKa = 10.26VNVVNAGTNKK97 pKa = 10.22DD98 pKa = 3.33QYY100 pKa = 10.65TALQNAISAGSGVPDD115 pKa = 3.65VAQIEE120 pKa = 4.97YY121 pKa = 10.29YY122 pKa = 11.1ALGQFTLSGALTEE135 pKa = 4.74LGSLGADD142 pKa = 3.03QLEE145 pKa = 5.14GTFSPGPWSAVQSGDD160 pKa = 3.89GIYY163 pKa = 10.74ALPTDD168 pKa = 4.68SGPMALFYY176 pKa = 11.15NKK178 pKa = 9.09TVFDD182 pKa = 3.54EE183 pKa = 4.7HH184 pKa = 8.34GIEE187 pKa = 5.82VPTTWEE193 pKa = 4.88GYY195 pKa = 10.31LEE197 pKa = 4.26AARR200 pKa = 11.84ALHH203 pKa = 6.23EE204 pKa = 5.2ADD206 pKa = 3.3PDD208 pKa = 3.6IYY210 pKa = 10.1ITNDD214 pKa = 3.0TGEE217 pKa = 4.51GGAATSMIWQAGGRR231 pKa = 11.84PYY233 pKa = 10.58TVDD236 pKa = 3.26GTDD239 pKa = 3.21VTIDD243 pKa = 3.72FSDD246 pKa = 3.79EE247 pKa = 3.65GTTAYY252 pKa = 9.53TDD254 pKa = 3.36LWQQLLDD261 pKa = 4.16EE262 pKa = 5.52DD263 pKa = 4.55LLAPITSWTDD273 pKa = 2.51EE274 pKa = 4.26WYY276 pKa = 10.5QGLGDD281 pKa = 4.26GSLATLATGAWMPPNFEE298 pKa = 4.26SGVPNAAGDD307 pKa = 3.54WRR309 pKa = 11.84AAPLPQWEE317 pKa = 4.25EE318 pKa = 3.98GANVSAEE325 pKa = 4.1NGGSSMAIPSAGKK338 pKa = 9.08NQALAYY344 pKa = 10.57AFVEE348 pKa = 4.2YY349 pKa = 10.83LNVGDD354 pKa = 4.05GVKK357 pKa = 10.35VRR359 pKa = 11.84TDD361 pKa = 2.95AGAFPATTADD371 pKa = 3.8LNSEE375 pKa = 4.16EE376 pKa = 4.22FLNTEE381 pKa = 4.21FPYY384 pKa = 10.75FGGQKK389 pKa = 10.18ANEE392 pKa = 4.06IFAEE396 pKa = 4.36SAANVGTGWSYY407 pKa = 11.68LPYY410 pKa = 10.01QVYY413 pKa = 10.9ANSIFNDD420 pKa = 3.8TAGQAYY426 pKa = 9.79ISGTTLADD434 pKa = 4.1GLKK437 pKa = 9.45AWQDD441 pKa = 3.01ASIAYY446 pKa = 9.8GKK448 pKa = 9.73EE449 pKa = 3.49QGFTVNDD456 pKa = 3.62

Molecular weight:
47.61 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0F7CP64|A0A0F7CP64_9ACTN Metal transporter ATPase OS=Streptomyces xiamenensis OX=408015 GN=SXIM_27320 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIITARR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.43GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight:
5.31 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5490

0

5490

1750436

37

6748

318.8

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.759 ± 0.056

0.743 ± 0.009

5.825 ± 0.028

6.006 ± 0.032

2.656 ± 0.018

9.754 ± 0.031

2.341 ± 0.018

3.327 ± 0.024

1.589 ± 0.021

10.588 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.719 ± 0.013

1.692 ± 0.023

6.372 ± 0.032

2.719 ± 0.022

8.326 ± 0.044

4.816 ± 0.023

6.216 ± 0.028

8.023 ± 0.032

1.504 ± 0.015

2.025 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski