Streptomyces xiamenensis 
Average proteome isoelectric point is 6.38 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 5490 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A0F7FZY1|A0A0F7FZY1_9ACTN Membrane protein OS=Streptomyces xiamenensis OX=408015 GN=SXIM_50610 PE=4 SV=1MM1 pKa = 7.76  DD2 pKa = 4.04  TMSNTTVRR10 pKa = 11.84  RR11 pKa = 11.84  LVRR14 pKa = 11.84  TTAVVGALALGVTACGSSDD33 pKa = 3.92  DD34 pKa = 4.16  GDD36 pKa = 4.04  GAGGGGGASSGLTPADD52 pKa = 3.12  VEE54 pKa = 4.26  AALEE58 pKa = 4.22  EE59 pKa = 4.85  GGSLTVWSWEE69 pKa = 3.85  ATLPAAAQAFMDD81 pKa = 3.49  EE82 pKa = 4.41  HH83 pKa = 6.91  PNVKK87 pKa = 10.26  VNVVNAGTNKK97 pKa = 10.22  DD98 pKa = 3.33  QYY100 pKa = 10.65  TALQNAISAGSGVPDD115 pKa = 3.65  VAQIEE120 pKa = 4.97  YY121 pKa = 10.29  YY122 pKa = 11.1  ALGQFTLSGALTEE135 pKa = 4.74  LGSLGADD142 pKa = 3.03  QLEE145 pKa = 5.14  GTFSPGPWSAVQSGDD160 pKa = 3.89  GIYY163 pKa = 10.74  ALPTDD168 pKa = 4.68  SGPMALFYY176 pKa = 11.15  NKK178 pKa = 9.09  TVFDD182 pKa = 3.54  EE183 pKa = 4.7  HH184 pKa = 8.34  GIEE187 pKa = 5.82  VPTTWEE193 pKa = 4.88  GYY195 pKa = 10.31  LEE197 pKa = 4.26  AARR200 pKa = 11.84  ALHH203 pKa = 6.23  EE204 pKa = 5.2  ADD206 pKa = 3.3  PDD208 pKa = 3.6  IYY210 pKa = 10.1  ITNDD214 pKa = 3.0  TGEE217 pKa = 4.51  GGAATSMIWQAGGRR231 pKa = 11.84  PYY233 pKa = 10.58  TVDD236 pKa = 3.26  GTDD239 pKa = 3.21  VTIDD243 pKa = 3.72  FSDD246 pKa = 3.79  EE247 pKa = 3.65  GTTAYY252 pKa = 9.53  TDD254 pKa = 3.36  LWQQLLDD261 pKa = 4.16  EE262 pKa = 5.52  DD263 pKa = 4.55  LLAPITSWTDD273 pKa = 2.51  EE274 pKa = 4.26  WYY276 pKa = 10.5  QGLGDD281 pKa = 4.26  GSLATLATGAWMPPNFEE298 pKa = 4.26  SGVPNAAGDD307 pKa = 3.54  WRR309 pKa = 11.84  AAPLPQWEE317 pKa = 4.25  EE318 pKa = 3.98  GANVSAEE325 pKa = 4.1  NGGSSMAIPSAGKK338 pKa = 9.08  NQALAYY344 pKa = 10.57  AFVEE348 pKa = 4.2  YY349 pKa = 10.83  LNVGDD354 pKa = 4.05  GVKK357 pKa = 10.35  VRR359 pKa = 11.84  TDD361 pKa = 2.95  AGAFPATTADD371 pKa = 3.8  LNSEE375 pKa = 4.16  EE376 pKa = 4.22  FLNTEE381 pKa = 4.21  FPYY384 pKa = 10.75  FGGQKK389 pKa = 10.18  ANEE392 pKa = 4.06  IFAEE396 pKa = 4.36  SAANVGTGWSYY407 pKa = 11.68  LPYY410 pKa = 10.01  QVYY413 pKa = 10.9  ANSIFNDD420 pKa = 3.8  TAGQAYY426 pKa = 9.79  ISGTTLADD434 pKa = 4.1  GLKK437 pKa = 9.45  AWQDD441 pKa = 3.01  ASIAYY446 pKa = 9.8  GKK448 pKa = 9.73  EE449 pKa = 3.49  QGFTVNDD456 pKa = 3.62  
 47.61 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.721 
IPC2_protein 3.706 
IPC_protein 3.719 
Toseland    3.516 
ProMoST     3.859 
Dawson      3.706 
Bjellqvist  3.884 
Wikipedia   3.63 
Rodwell     3.554 
Grimsley    3.414 
Solomon     3.706 
Lehninger   3.656 
Nozaki      3.821 
DTASelect   4.037 
Thurlkill   3.554 
EMBOSS      3.643 
Sillero     3.846 
Patrickios  0.795 
IPC_peptide 3.694 
IPC2_peptide  3.821 
IPC2.peptide.svr19  3.764 
 Protein with the highest isoelectric point: 
>tr|A0A0F7CP64|A0A0F7CP64_9ACTN Metal transporter ATPase OS=Streptomyces xiamenensis OX=408015 GN=SXIM_27320 PE=3 SV=1MM1 pKa = 7.69  SKK3 pKa = 9.0  RR4 pKa = 11.84  TFQPNNRR11 pKa = 11.84  RR12 pKa = 11.84  RR13 pKa = 11.84  AKK15 pKa = 8.7  THH17 pKa = 5.15  GFRR20 pKa = 11.84  LRR22 pKa = 11.84  MRR24 pKa = 11.84  TRR26 pKa = 11.84  AGRR29 pKa = 11.84  AIITARR35 pKa = 11.84  RR36 pKa = 11.84  TKK38 pKa = 10.43  GRR40 pKa = 11.84  ARR42 pKa = 11.84  LSAA45 pKa = 3.91  
 5.31 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.527 
IPC2_protein 11.316 
IPC_protein 12.925 
Toseland    13.086 
ProMoST     13.583 
Dawson      13.086 
Bjellqvist  13.086 
Wikipedia   13.554 
Rodwell     12.676 
Grimsley    13.13 
Solomon     13.583 
Lehninger   13.481 
Nozaki      13.086 
DTASelect   13.086 
Thurlkill   13.086 
EMBOSS      13.583 
Sillero     13.086 
Patrickios  12.398 
IPC_peptide 13.583 
IPC2_peptide  12.574 
IPC2.peptide.svr19  9.243 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        5490 
0
5490 
1750436
37
6748
318.8
34.09
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        13.759 ± 0.056
0.743 ± 0.009
5.825 ± 0.028
6.006 ± 0.032
2.656 ± 0.018
9.754 ± 0.031
2.341 ± 0.018
3.327 ± 0.024
1.589 ± 0.021
10.588 ± 0.037
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.719 ± 0.013
1.692 ± 0.023
6.372 ± 0.032
2.719 ± 0.022
8.326 ± 0.044
4.816 ± 0.023
6.216 ± 0.028
8.023 ± 0.032
1.504 ± 0.015
2.025 ± 0.016
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here