Streptomyces xiamenensis
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5490 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F7FZY1|A0A0F7FZY1_9ACTN Membrane protein OS=Streptomyces xiamenensis OX=408015 GN=SXIM_50610 PE=4 SV=1
MM1 pKa = 7.76 DD2 pKa = 4.04 TMSNTTVRR10 pKa = 11.84 RR11 pKa = 11.84 LVRR14 pKa = 11.84 TTAVVGALALGVTACGSSDD33 pKa = 3.92 DD34 pKa = 4.16 GDD36 pKa = 4.04 GAGGGGGASSGLTPADD52 pKa = 3.12 VEE54 pKa = 4.26 AALEE58 pKa = 4.22 EE59 pKa = 4.85 GGSLTVWSWEE69 pKa = 3.85 ATLPAAAQAFMDD81 pKa = 3.49 EE82 pKa = 4.41 HH83 pKa = 6.91 PNVKK87 pKa = 10.26 VNVVNAGTNKK97 pKa = 10.22 DD98 pKa = 3.33 QYY100 pKa = 10.65 TALQNAISAGSGVPDD115 pKa = 3.65 VAQIEE120 pKa = 4.97 YY121 pKa = 10.29 YY122 pKa = 11.1 ALGQFTLSGALTEE135 pKa = 4.74 LGSLGADD142 pKa = 3.03 QLEE145 pKa = 5.14 GTFSPGPWSAVQSGDD160 pKa = 3.89 GIYY163 pKa = 10.74 ALPTDD168 pKa = 4.68 SGPMALFYY176 pKa = 11.15 NKK178 pKa = 9.09 TVFDD182 pKa = 3.54 EE183 pKa = 4.7 HH184 pKa = 8.34 GIEE187 pKa = 5.82 VPTTWEE193 pKa = 4.88 GYY195 pKa = 10.31 LEE197 pKa = 4.26 AARR200 pKa = 11.84 ALHH203 pKa = 6.23 EE204 pKa = 5.2 ADD206 pKa = 3.3 PDD208 pKa = 3.6 IYY210 pKa = 10.1 ITNDD214 pKa = 3.0 TGEE217 pKa = 4.51 GGAATSMIWQAGGRR231 pKa = 11.84 PYY233 pKa = 10.58 TVDD236 pKa = 3.26 GTDD239 pKa = 3.21 VTIDD243 pKa = 3.72 FSDD246 pKa = 3.79 EE247 pKa = 3.65 GTTAYY252 pKa = 9.53 TDD254 pKa = 3.36 LWQQLLDD261 pKa = 4.16 EE262 pKa = 5.52 DD263 pKa = 4.55 LLAPITSWTDD273 pKa = 2.51 EE274 pKa = 4.26 WYY276 pKa = 10.5 QGLGDD281 pKa = 4.26 GSLATLATGAWMPPNFEE298 pKa = 4.26 SGVPNAAGDD307 pKa = 3.54 WRR309 pKa = 11.84 AAPLPQWEE317 pKa = 4.25 EE318 pKa = 3.98 GANVSAEE325 pKa = 4.1 NGGSSMAIPSAGKK338 pKa = 9.08 NQALAYY344 pKa = 10.57 AFVEE348 pKa = 4.2 YY349 pKa = 10.83 LNVGDD354 pKa = 4.05 GVKK357 pKa = 10.35 VRR359 pKa = 11.84 TDD361 pKa = 2.95 AGAFPATTADD371 pKa = 3.8 LNSEE375 pKa = 4.16 EE376 pKa = 4.22 FLNTEE381 pKa = 4.21 FPYY384 pKa = 10.75 FGGQKK389 pKa = 10.18 ANEE392 pKa = 4.06 IFAEE396 pKa = 4.36 SAANVGTGWSYY407 pKa = 11.68 LPYY410 pKa = 10.01 QVYY413 pKa = 10.9 ANSIFNDD420 pKa = 3.8 TAGQAYY426 pKa = 9.79 ISGTTLADD434 pKa = 4.1 GLKK437 pKa = 9.45 AWQDD441 pKa = 3.01 ASIAYY446 pKa = 9.8 GKK448 pKa = 9.73 EE449 pKa = 3.49 QGFTVNDD456 pKa = 3.62
Molecular weight: 47.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.516
ProMoST 3.859
Dawson 3.706
Bjellqvist 3.884
Wikipedia 3.63
Rodwell 3.554
Grimsley 3.414
Solomon 3.706
Lehninger 3.656
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.554
EMBOSS 3.643
Sillero 3.846
Patrickios 0.795
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A0F7CP64|A0A0F7CP64_9ACTN Metal transporter ATPase OS=Streptomyces xiamenensis OX=408015 GN=SXIM_27320 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIITARR35 pKa = 11.84 RR36 pKa = 11.84 TKK38 pKa = 10.43 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5490
0
5490
1750436
37
6748
318.8
34.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.759 ± 0.056
0.743 ± 0.009
5.825 ± 0.028
6.006 ± 0.032
2.656 ± 0.018
9.754 ± 0.031
2.341 ± 0.018
3.327 ± 0.024
1.589 ± 0.021
10.588 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.719 ± 0.013
1.692 ± 0.023
6.372 ± 0.032
2.719 ± 0.022
8.326 ± 0.044
4.816 ± 0.023
6.216 ± 0.028
8.023 ± 0.032
1.504 ± 0.015
2.025 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here