Gordonia effusa NBRC 100432

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; Gordonia effusa

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4419 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0R1B4|H0R1B4_9ACTN Homoserine O-acetyltransferase OS=Gordonia effusa NBRC 100432 OX=1077974 GN=metA PE=3 SV=1
MM1 pKa = 7.47SALSQLTDD9 pKa = 3.8LVDD12 pKa = 4.33PPPHH16 pKa = 6.63GARR19 pKa = 11.84PDD21 pKa = 3.35WTAVAAHH28 pKa = 7.18LGHH31 pKa = 6.97TPPQDD36 pKa = 3.47YY37 pKa = 10.46IQIVEE42 pKa = 4.43TYY44 pKa = 10.47GAGLFAGEE52 pKa = 4.06VAVWVAGGAGGEE64 pKa = 4.25DD65 pKa = 4.69LIEE68 pKa = 4.34AAPPAITEE76 pKa = 4.32LADD79 pKa = 3.31SRR81 pKa = 11.84DD82 pKa = 3.93WINSNAISWIGPDD95 pKa = 3.72GSNSPVDD102 pKa = 4.56LGPSPLRR109 pKa = 11.84YY110 pKa = 9.22AAWGGGSSGAYY121 pKa = 9.95GYY123 pKa = 8.51WHH125 pKa = 6.34QVGDD129 pKa = 5.3DD130 pKa = 3.79PNKK133 pKa = 9.98WPVLYY138 pKa = 10.18TDD140 pKa = 5.59LSSLWLYY147 pKa = 10.62HH148 pKa = 6.31PAGIAAFLVDD158 pKa = 5.74LLDD161 pKa = 4.07GNYY164 pKa = 8.43NTEE167 pKa = 4.67LIEE170 pKa = 4.19LSPDD174 pKa = 3.05EE175 pKa = 5.32RR176 pKa = 11.84PDD178 pKa = 3.66FEE180 pKa = 6.29AFGSDD185 pKa = 2.97

Molecular weight: 19.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0R4K4|H0R4K4_9ACTN Manganese ABC transporter substrate binding protein OS=Gordonia effusa NBRC 100432 OX=1077974 GN=mntC PE=3 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84CGTIRR7 pKa = 11.84LGRR10 pKa = 11.84LTILRR15 pKa = 11.84LLTVLGLLTILRR27 pKa = 11.84LLTILGLLTILRR39 pKa = 11.84LLATLWLLTLRR50 pKa = 11.84IVVIAWGAIRR60 pKa = 11.84ARR62 pKa = 11.84HH63 pKa = 5.58RR64 pKa = 11.84RR65 pKa = 11.84VLSNN69 pKa = 3.31

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4419

0

4419

1423384

36

7894

322.1

34.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.646 ± 0.047

0.696 ± 0.011

6.492 ± 0.032

5.002 ± 0.037

3.061 ± 0.021

8.642 ± 0.037

2.049 ± 0.017

4.859 ± 0.023

2.465 ± 0.028

9.554 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.975 ± 0.015

2.425 ± 0.017

5.392 ± 0.028

2.918 ± 0.02

6.956 ± 0.03

6.142 ± 0.025

6.398 ± 0.027

8.735 ± 0.037

1.444 ± 0.017

2.15 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski