Parry s Lagoon virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; unclassified Orbivirus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173G2X3|A0A173G2X3_9REOV RNA-directed RNA polymerase OS=Parry's Lagoon virus OX=1853202 PE=3 SV=1
MM1 pKa = 7.55GKK3 pKa = 8.06ITNALSKK10 pKa = 10.89FGGAVSKK17 pKa = 10.63AVKK20 pKa = 10.57SNTAKK25 pKa = 10.79KK26 pKa = 9.73IFKK29 pKa = 10.14AAIDD33 pKa = 3.79GAGRR37 pKa = 11.84VAQSEE42 pKa = 4.23LGQRR46 pKa = 11.84AISGLIEE53 pKa = 4.32GAATAALTDD62 pKa = 3.73GSYY65 pKa = 11.43GEE67 pKa = 4.21EE68 pKa = 3.78IKK70 pKa = 10.82RR71 pKa = 11.84AVILNIAGVNDD82 pKa = 3.94VVTDD86 pKa = 3.78PLNPIEE92 pKa = 4.77HH93 pKa = 7.1ALAQKK98 pKa = 10.27VNQIDD103 pKa = 3.18KK104 pKa = 10.8VMRR107 pKa = 11.84SNADD111 pKa = 2.48IDD113 pKa = 4.09KK114 pKa = 10.36YY115 pKa = 11.31GKK117 pKa = 8.83VLKK120 pKa = 10.55RR121 pKa = 11.84IDD123 pKa = 4.21GIGAEE128 pKa = 4.12LTKK131 pKa = 10.56VEE133 pKa = 4.68KK134 pKa = 10.61YY135 pKa = 10.63LQISHH140 pKa = 6.67DD141 pKa = 3.99TEE143 pKa = 3.75ISEE146 pKa = 4.24QEE148 pKa = 3.94EE149 pKa = 4.34IEE151 pKa = 4.13ALEE154 pKa = 4.28AAMKK158 pKa = 10.6AMGMVVGEE166 pKa = 4.28EE167 pKa = 4.09KK168 pKa = 10.78KK169 pKa = 10.81NLAILEE175 pKa = 4.15KK176 pKa = 10.52ALRR179 pKa = 11.84KK180 pKa = 8.56EE181 pKa = 3.87DD182 pKa = 3.37RR183 pKa = 11.84MRR185 pKa = 11.84SADD188 pKa = 3.98EE189 pKa = 3.81KK190 pKa = 11.53RR191 pKa = 11.84MIEE194 pKa = 4.04YY195 pKa = 8.41MKK197 pKa = 10.63RR198 pKa = 11.84NYY200 pKa = 9.47EE201 pKa = 4.03HH202 pKa = 7.12LAEE205 pKa = 4.45IANKK209 pKa = 9.05EE210 pKa = 3.93KK211 pKa = 10.92EE212 pKa = 4.42SIIEE216 pKa = 3.89EE217 pKa = 4.13ALEE220 pKa = 3.81QTIDD224 pKa = 3.14IGGEE228 pKa = 3.84IAEE231 pKa = 4.38HH232 pKa = 6.41LAAEE236 pKa = 4.41VPLVGEE242 pKa = 4.71GVAAGMATARR252 pKa = 11.84GAVQIYY258 pKa = 10.48KK259 pKa = 9.93LGKK262 pKa = 10.0VISDD266 pKa = 3.75LTNTPIHH273 pKa = 6.2HH274 pKa = 6.82VEE276 pKa = 4.29LPMITPEE283 pKa = 4.12GLQVLYY289 pKa = 10.54EE290 pKa = 4.26EE291 pKa = 5.23PNPTEE296 pKa = 4.18DD297 pKa = 3.65QNLLRR302 pKa = 11.84IVSSKK307 pKa = 10.24LKK309 pKa = 10.52HH310 pKa = 6.19VEE312 pKa = 4.0EE313 pKa = 4.12VDD315 pKa = 3.55KK316 pKa = 11.26EE317 pKa = 4.5VVHH320 pKa = 7.28LSEE323 pKa = 4.24KK324 pKa = 10.18VVPAVVKK331 pKa = 10.21QAATDD336 pKa = 3.72SLEE339 pKa = 4.44LGGTGKK345 pKa = 10.39GVPMRR350 pKa = 11.84TRR352 pKa = 11.84ASNHH356 pKa = 4.21VPRR359 pKa = 11.84NQRR362 pKa = 11.84PAIHH366 pKa = 7.14FYY368 pKa = 8.81TAPWDD373 pKa = 3.55SDD375 pKa = 3.5FVIILHH381 pKa = 6.25VIAPYY386 pKa = 10.71SSDD389 pKa = 3.1ASFVLALDD397 pKa = 4.41LATDD401 pKa = 3.61YY402 pKa = 11.35VGYY405 pKa = 10.69YY406 pKa = 10.18DD407 pKa = 3.75IYY409 pKa = 10.7KK410 pKa = 10.27DD411 pKa = 3.99YY412 pKa = 11.57VSDD415 pKa = 4.06CPGVDD420 pKa = 3.1SYY422 pKa = 12.18FNLQHH427 pKa = 6.82AVDD430 pKa = 4.71DD431 pKa = 4.33FLIEE435 pKa = 3.97ASAVGGSTEE444 pKa = 3.46IHH446 pKa = 6.68AEE448 pKa = 3.79RR449 pKa = 11.84LQRR452 pKa = 11.84GVGTSAIYY460 pKa = 10.5VGSQEE465 pKa = 3.85YY466 pKa = 9.56RR467 pKa = 11.84VSFEE471 pKa = 4.54AMRR474 pKa = 11.84EE475 pKa = 3.81HH476 pKa = 7.69AKK478 pKa = 10.57RR479 pKa = 11.84IVQDD483 pKa = 3.6SSIQMHH489 pKa = 6.92LLRR492 pKa = 11.84GPLSMQRR499 pKa = 11.84TSLLNALMHH508 pKa = 6.61GVTILNDD515 pKa = 3.62TPTGGMTTQCTCC527 pKa = 4.01

Molecular weight: 57.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A173G2X7|A0A173G2X7_9REOV Non-structural protein NS3 OS=Parry's Lagoon virus OX=1853202 PE=3 SV=1
MM1 pKa = 7.47LALADD6 pKa = 3.97SLVGKK11 pKa = 9.99GPKK14 pKa = 9.16QDD16 pKa = 3.29VVVTVVPPEE25 pKa = 3.94KK26 pKa = 10.4FGLLKK31 pKa = 10.19DD32 pKa = 3.74TSNEE36 pKa = 3.53EE37 pKa = 3.82RR38 pKa = 11.84DD39 pKa = 3.29RR40 pKa = 11.84DD41 pKa = 3.66GALSVLTNAIVSATGTNEE59 pKa = 3.79TQKK62 pKa = 10.88NEE64 pKa = 3.83KK65 pKa = 9.02ATFGAISEE73 pKa = 4.28ALKK76 pKa = 10.72DD77 pKa = 3.86DD78 pKa = 3.55NVTRR82 pKa = 11.84RR83 pKa = 11.84SKK85 pKa = 10.69KK86 pKa = 8.29IAYY89 pKa = 8.19SKK91 pKa = 11.08SVFEE95 pKa = 4.37LEE97 pKa = 3.61KK98 pKa = 10.67DD99 pKa = 2.72IRR101 pKa = 11.84RR102 pKa = 11.84YY103 pKa = 10.59RR104 pKa = 11.84NTIRR108 pKa = 11.84LLEE111 pKa = 4.2CARR114 pKa = 11.84VGCAIVVLLFSAALSIMDD132 pKa = 4.72FLSKK136 pKa = 10.91DD137 pKa = 4.14VITKK141 pKa = 8.16MATFVGDD148 pKa = 3.33HH149 pKa = 6.58GSADD153 pKa = 3.55FAAMTRR159 pKa = 11.84LRR161 pKa = 11.84AIRR164 pKa = 11.84GAVNMALITCGTAVLITGRR183 pKa = 11.84NVAIMKK189 pKa = 8.81QKK191 pKa = 10.16KK192 pKa = 9.41RR193 pKa = 11.84GMKK196 pKa = 9.57RR197 pKa = 11.84DD198 pKa = 3.39LVKK201 pKa = 10.8RR202 pKa = 11.84KK203 pKa = 9.8AYY205 pKa = 10.55LGAIDD210 pKa = 5.9DD211 pKa = 4.5MNLLTPYY218 pKa = 9.68AIEE221 pKa = 4.43MDD223 pKa = 4.08PPTYY227 pKa = 10.54AAAKK231 pKa = 10.12APYY234 pKa = 9.75EE235 pKa = 3.99YY236 pKa = 11.06DD237 pKa = 3.18LL238 pKa = 5.4

Molecular weight: 26.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6043

238

1290

604.3

68.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.314 ± 0.717

1.092 ± 0.205

6.619 ± 0.363

6.354 ± 0.406

3.376 ± 0.372

5.891 ± 0.391

2.664 ± 0.198

6.272 ± 0.367

5.891 ± 0.661

8.804 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.161 ± 0.223

3.756 ± 0.307

4.154 ± 0.315

3.227 ± 0.293

6.851 ± 0.426

6.47 ± 0.446

5.941 ± 0.387

7.083 ± 0.422

1.191 ± 0.252

3.872 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski