Arcticibacter pallidicorallinus
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3484 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T0U569|A0A2T0U569_9SPHI Uncharacterized protein OS=Arcticibacter pallidicorallinus OX=1259464 GN=B0I27_10474 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.21 LASHH10 pKa = 7.01 LEE12 pKa = 4.08 DD13 pKa = 4.93 APWPATKK20 pKa = 10.47 DD21 pKa = 3.38 EE22 pKa = 5.13 LIDD25 pKa = 3.42 YY26 pKa = 7.68 GIRR29 pKa = 11.84 SGAPVEE35 pKa = 4.53 VIEE38 pKa = 4.56 NLQALEE44 pKa = 4.69 DD45 pKa = 4.28 DD46 pKa = 4.31 GEE48 pKa = 4.31 PYY50 pKa = 11.05 EE51 pKa = 4.65 NIEE54 pKa = 4.81 EE55 pKa = 4.08 IWPDD59 pKa = 3.85 YY60 pKa = 7.47 PTKK63 pKa = 10.94 DD64 pKa = 3.05 DD65 pKa = 4.15 FFFNEE70 pKa = 4.51 DD71 pKa = 3.17 EE72 pKa = 4.46 YY73 pKa = 11.99
Molecular weight: 8.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.818
IPC2_protein 3.592
IPC_protein 3.528
Toseland 3.338
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.439
Rodwell 3.363
Grimsley 3.261
Solomon 3.478
Lehninger 3.439
Nozaki 3.643
DTASelect 3.795
Thurlkill 3.401
EMBOSS 3.452
Sillero 3.656
Patrickios 0.693
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.629
Protein with the highest isoelectric point:
>tr|A0A2T0U4F6|A0A2T0U4F6_9SPHI F420_oxidored domain-containing protein OS=Arcticibacter pallidicorallinus OX=1259464 GN=B0I27_105239 PE=4 SV=1
MM1 pKa = 7.47 YY2 pKa = 10.34 IFLLSLHH9 pKa = 6.39 SLFRR13 pKa = 11.84 WLLLGSLLCSIIFSYY28 pKa = 10.83 RR29 pKa = 11.84 SLLRR33 pKa = 11.84 QSVFTPIADD42 pKa = 4.37 RR43 pKa = 11.84 LRR45 pKa = 11.84 HH46 pKa = 4.4 WTATIAHH53 pKa = 6.06 IQLLIGMTLYY63 pKa = 10.2 FQSPIVMFEE72 pKa = 3.88 MFEE75 pKa = 4.63 SNGRR79 pKa = 11.84 LWTEE83 pKa = 3.76 HH84 pKa = 4.98 NFYY87 pKa = 10.56 RR88 pKa = 11.84 YY89 pKa = 9.0 VHH91 pKa = 6.55 ISLMISAVVMITIGSAKK108 pKa = 9.97 AKK110 pKa = 10.19 RR111 pKa = 11.84 RR112 pKa = 11.84 ADD114 pKa = 3.5 DD115 pKa = 3.29 QAKK118 pKa = 10.07 YY119 pKa = 9.1 RR120 pKa = 11.84 TILRR124 pKa = 11.84 WFILALLIIFFAIPWPFSPFASRR147 pKa = 11.84 PLFRR151 pKa = 11.84 HH152 pKa = 5.96 FF153 pKa = 4.35
Molecular weight: 18.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 10.043
IPC_protein 10.979
Toseland 10.687
ProMoST 10.643
Dawson 10.847
Bjellqvist 10.672
Wikipedia 11.155
Rodwell 10.847
Grimsley 10.935
Solomon 11.008
Lehninger 10.95
Nozaki 10.672
DTASelect 10.672
Thurlkill 10.73
EMBOSS 11.111
Sillero 10.789
Patrickios 10.526
IPC_peptide 11.008
IPC2_peptide 9.867
IPC2.peptide.svr19 8.109
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3484
0
3484
1264185
27
2976
362.9
40.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.363 ± 0.038
0.769 ± 0.011
5.426 ± 0.028
6.066 ± 0.043
4.818 ± 0.028
7.057 ± 0.047
1.864 ± 0.022
7.023 ± 0.032
6.528 ± 0.035
9.494 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.309 ± 0.018
5.189 ± 0.034
3.846 ± 0.024
3.516 ± 0.023
4.58 ± 0.026
7.016 ± 0.033
5.416 ± 0.039
6.513 ± 0.03
1.21 ± 0.019
3.999 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here