Corynebacterium phage Lederberg
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y6EXC4|A0A4Y6EXC4_9CAUD Uncharacterized protein OS=Corynebacterium phage Lederberg OX=2588502 GN=47 PE=4 SV=1
MM1 pKa = 7.88 TDD3 pKa = 3.23 PDD5 pKa = 4.4 FPDD8 pKa = 3.66 SYY10 pKa = 11.23 KK11 pKa = 10.64 LLHH14 pKa = 6.18 GAPYY18 pKa = 10.72 DD19 pKa = 4.53 DD20 pKa = 6.43 DD21 pKa = 5.09 PGPWPYY27 pKa = 10.86 LAVIAFAAIAAVAILVASYY46 pKa = 10.82 LL47 pKa = 3.74
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.84
IPC2_protein 3.757
IPC_protein 3.617
Toseland 3.414
ProMoST 3.884
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.732
Rodwell 3.478
Grimsley 3.35
Solomon 3.617
Lehninger 3.579
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.541
EMBOSS 3.732
Sillero 3.77
Patrickios 0.172
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A4Y6EXB5|A0A4Y6EXB5_9CAUD Uncharacterized protein OS=Corynebacterium phage Lederberg OX=2588502 GN=37 PE=4 SV=1
MM1 pKa = 8.05 PEE3 pKa = 3.79 TTNRR7 pKa = 11.84 PTPHH11 pKa = 7.17 ALDD14 pKa = 3.63 SMRR17 pKa = 11.84 GGAGATAPTGGSKK30 pKa = 10.93 DD31 pKa = 3.81 EE32 pKa = 4.21 ITVSVQRR39 pKa = 11.84 RR40 pKa = 11.84 EE41 pKa = 3.63 RR42 pKa = 11.84 GGRR45 pKa = 11.84 VRR47 pKa = 11.84 WIGRR51 pKa = 11.84 YY52 pKa = 9.18 RR53 pKa = 11.84 GLDD56 pKa = 3.02 GKK58 pKa = 10.34 EE59 pKa = 3.48 RR60 pKa = 11.84 SRR62 pKa = 11.84 SFEE65 pKa = 3.7 KK66 pKa = 10.72 RR67 pKa = 11.84 RR68 pKa = 11.84 DD69 pKa = 3.41 AVTWVAEE76 pKa = 4.44 KK77 pKa = 10.75 KK78 pKa = 9.81 RR79 pKa = 11.84 DD80 pKa = 3.58 LRR82 pKa = 11.84 RR83 pKa = 11.84 GDD85 pKa = 3.58 WIDD88 pKa = 3.39 PAEE91 pKa = 4.02 QKK93 pKa = 8.97 ITIGEE98 pKa = 4.26 LARR101 pKa = 11.84 EE102 pKa = 4.07 RR103 pKa = 11.84 TMMARR108 pKa = 11.84 TTNTKK113 pKa = 7.59 ATRR116 pKa = 11.84 RR117 pKa = 11.84 FFEE120 pKa = 4.82 ANLGPLEE127 pKa = 4.3 GAAIGTVRR135 pKa = 11.84 ASHH138 pKa = 5.1 MRR140 pKa = 11.84 EE141 pKa = 3.37 WHH143 pKa = 5.06 TMLRR147 pKa = 11.84 NGRR150 pKa = 11.84 PWQSGSLSPATIDD163 pKa = 3.34 GLTVQMRR170 pKa = 11.84 TLMKK174 pKa = 10.35 QAVADD179 pKa = 4.09 GLISRR184 pKa = 11.84 SPAAGLEE191 pKa = 4.11 RR192 pKa = 11.84 VGRR195 pKa = 11.84 DD196 pKa = 3.14 VVVIPRR202 pKa = 11.84 DD203 pKa = 3.26 IPTPAEE209 pKa = 3.78 VGALIRR215 pKa = 11.84 AFEE218 pKa = 4.07 TGIRR222 pKa = 11.84 APEE225 pKa = 4.02 PLVPQYY231 pKa = 10.97 RR232 pKa = 11.84 RR233 pKa = 11.84 DD234 pKa = 3.42 GRR236 pKa = 11.84 RR237 pKa = 11.84 KK238 pKa = 8.85 PRR240 pKa = 11.84 GWLLKK245 pKa = 8.91 PAPWMARR252 pKa = 11.84 AVRR255 pKa = 11.84 LAIATGMRR263 pKa = 11.84 PSEE266 pKa = 4.07 VAGMRR271 pKa = 11.84 VRR273 pKa = 11.84 SLDD276 pKa = 3.44 QLTGSVDD283 pKa = 3.69 VVEE286 pKa = 4.36 QAARR290 pKa = 11.84 HH291 pKa = 4.38 STEE294 pKa = 3.8 TVEE297 pKa = 5.32 LKK299 pKa = 10.07 TEE301 pKa = 4.38 ASRR304 pKa = 11.84 RR305 pKa = 11.84 WLPVDD310 pKa = 3.92 AEE312 pKa = 4.42 TLHH315 pKa = 7.18 VLVDD319 pKa = 3.7 WAQDD323 pKa = 3.18 RR324 pKa = 11.84 DD325 pKa = 3.94 LGPDD329 pKa = 2.8 ARR331 pKa = 11.84 LFHH334 pKa = 6.45 VRR336 pKa = 11.84 GGNPATAEE344 pKa = 4.37 AIGRR348 pKa = 11.84 LMRR351 pKa = 11.84 SAVDD355 pKa = 3.19 AGVWRR360 pKa = 11.84 GGMTFHH366 pKa = 7.62 SLRR369 pKa = 11.84 HH370 pKa = 4.92 FHH372 pKa = 7.06 ASALIAAGLDD382 pKa = 3.32 VAVVSRR388 pKa = 11.84 RR389 pKa = 11.84 LGHH392 pKa = 6.35 EE393 pKa = 3.99 NMSTTLEE400 pKa = 4.57 VYY402 pKa = 8.49 THH404 pKa = 7.03 LWPGSEE410 pKa = 3.9 EE411 pKa = 4.31 RR412 pKa = 11.84 EE413 pKa = 4.0 RR414 pKa = 11.84 EE415 pKa = 3.8 AATALVRR422 pKa = 11.84 DD423 pKa = 3.83 VCGTGGRR430 pKa = 11.84 GEE432 pKa = 4.48 GSDD435 pKa = 3.5 SGSVQVSS442 pKa = 3.17
Molecular weight: 48.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.399
IPC_protein 10.496
Toseland 10.804
ProMoST 10.833
Dawson 10.847
Bjellqvist 10.657
Wikipedia 11.155
Rodwell 10.804
Grimsley 10.891
Solomon 11.125
Lehninger 11.067
Nozaki 10.789
DTASelect 10.657
Thurlkill 10.804
EMBOSS 11.242
Sillero 10.804
Patrickios 10.511
IPC_peptide 11.14
IPC2_peptide 9.794
IPC2.peptide.svr19 9.012
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
14027
45
1803
233.8
25.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.775 ± 0.663
0.606 ± 0.103
7.065 ± 0.38
6.138 ± 0.261
2.452 ± 0.194
8.598 ± 0.518
2.424 ± 0.277
4.869 ± 0.192
3.301 ± 0.228
7.721 ± 0.357
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.445 ± 0.112
2.438 ± 0.185
5.789 ± 0.385
3.45 ± 0.189
7.115 ± 0.467
4.748 ± 0.412
6.794 ± 0.318
6.88 ± 0.231
2.231 ± 0.15
2.16 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here