Amino acid dipepetide frequency for Corynebacterium phage Lederberg

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.321AlaAla: 19.321 ± 2.412
0.499AlaCys: 0.499 ± 0.188
9.34AlaAsp: 9.34 ± 1.123
9.625AlaGlu: 9.625 ± 1.005
2.852AlaPhe: 2.852 ± 0.599
11.265AlaGly: 11.265 ± 1.153
2.068AlaHis: 2.068 ± 0.386
6.844AlaIle: 6.844 ± 0.691
3.636AlaLys: 3.636 ± 0.661
10.195AlaLeu: 10.195 ± 0.801
3.422AlaMet: 3.422 ± 0.571
2.781AlaAsn: 2.781 ± 0.622
7.13AlaPro: 7.13 ± 0.653
4.777AlaGln: 4.777 ± 0.757
8.128AlaArg: 8.128 ± 0.745
4.991AlaSer: 4.991 ± 0.804
7.058AlaThr: 7.058 ± 0.727
8.484AlaVal: 8.484 ± 0.846
2.353AlaTrp: 2.353 ± 0.543
2.994AlaTyr: 2.994 ± 0.548
0.0AlaXaa: 0.0 ± 0.0
Cys
0.356CysAla: 0.356 ± 0.205
0.143CysCys: 0.143 ± 0.1
0.356CysAsp: 0.356 ± 0.164
0.356CysGlu: 0.356 ± 0.144
0.214CysPhe: 0.214 ± 0.16
0.499CysGly: 0.499 ± 0.168
0.143CysHis: 0.143 ± 0.128
0.214CysIle: 0.214 ± 0.111
0.214CysLys: 0.214 ± 0.109
0.57CysLeu: 0.57 ± 0.177
0.0CysMet: 0.0 ± 0.0
0.356CysAsn: 0.356 ± 0.157
0.214CysPro: 0.214 ± 0.132
0.285CysGln: 0.285 ± 0.156
0.428CysArg: 0.428 ± 0.158
0.428CysSer: 0.428 ± 0.163
0.57CysThr: 0.57 ± 0.204
0.356CysVal: 0.356 ± 0.182
0.214CysTrp: 0.214 ± 0.118
0.143CysTyr: 0.143 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
10.481AspAla: 10.481 ± 0.764
0.285AspCys: 0.285 ± 0.126
5.276AspAsp: 5.276 ± 0.87
5.133AspGlu: 5.133 ± 0.659
1.569AspPhe: 1.569 ± 0.361
7.629AspGly: 7.629 ± 0.782
2.068AspHis: 2.068 ± 0.464
2.424AspIle: 2.424 ± 0.538
1.569AspLys: 1.569 ± 0.521
5.561AspLeu: 5.561 ± 0.66
1.283AspMet: 1.283 ± 0.315
1.283AspAsn: 1.283 ± 0.303
4.206AspPro: 4.206 ± 0.713
3.208AspGln: 3.208 ± 0.566
4.064AspArg: 4.064 ± 0.657
2.852AspSer: 2.852 ± 0.506
3.921AspThr: 3.921 ± 0.816
4.349AspVal: 4.349 ± 0.579
1.996AspTrp: 1.996 ± 0.3
1.497AspTyr: 1.497 ± 0.334
0.0AspXaa: 0.0 ± 0.0
Glu
6.131GluAla: 6.131 ± 0.505
0.356GluCys: 0.356 ± 0.148
2.781GluAsp: 2.781 ± 0.462
3.636GluGlu: 3.636 ± 0.628
1.782GluPhe: 1.782 ± 0.395
3.779GluGly: 3.779 ± 0.491
1.854GluHis: 1.854 ± 0.35
3.422GluIle: 3.422 ± 0.53
2.567GluLys: 2.567 ± 0.439
5.561GluLeu: 5.561 ± 0.669
1.283GluMet: 1.283 ± 0.344
1.497GluAsn: 1.497 ± 0.286
4.278GluPro: 4.278 ± 0.885
3.351GluGln: 3.351 ± 0.335
4.42GluArg: 4.42 ± 0.65
3.208GluSer: 3.208 ± 0.411
3.707GluThr: 3.707 ± 0.478
4.206GluVal: 4.206 ± 0.514
1.283GluTrp: 1.283 ± 0.306
2.281GluTyr: 2.281 ± 0.412
0.0GluXaa: 0.0 ± 0.0
Phe
1.925PheAla: 1.925 ± 0.367
0.285PheCys: 0.285 ± 0.124
2.21PheAsp: 2.21 ± 0.415
1.569PheGlu: 1.569 ± 0.415
0.998PhePhe: 0.998 ± 0.368
2.923PheGly: 2.923 ± 0.453
0.713PheHis: 0.713 ± 0.296
1.426PheIle: 1.426 ± 0.321
0.856PheLys: 0.856 ± 0.23
1.925PheLeu: 1.925 ± 0.392
0.428PheMet: 0.428 ± 0.147
0.428PheAsn: 0.428 ± 0.147
0.927PhePro: 0.927 ± 0.271
0.642PheGln: 0.642 ± 0.189
1.426PheArg: 1.426 ± 0.405
1.212PheSer: 1.212 ± 0.415
1.996PheThr: 1.996 ± 0.518
1.426PheVal: 1.426 ± 0.364
0.713PheTrp: 0.713 ± 0.192
0.499PheTyr: 0.499 ± 0.157
0.0PheXaa: 0.0 ± 0.0
Gly
8.342GlyAla: 8.342 ± 1.068
0.428GlyCys: 0.428 ± 0.168
5.704GlyAsp: 5.704 ± 0.625
4.991GlyGlu: 4.991 ± 0.607
2.709GlyPhe: 2.709 ± 0.46
7.557GlyGly: 7.557 ± 0.874
2.781GlyHis: 2.781 ± 0.482
4.064GlyIle: 4.064 ± 0.768
4.206GlyLys: 4.206 ± 0.64
6.131GlyLeu: 6.131 ± 0.641
3.137GlyMet: 3.137 ± 0.517
2.923GlyAsn: 2.923 ± 0.587
4.135GlyPro: 4.135 ± 0.647
2.567GlyGln: 2.567 ± 0.408
6.274GlyArg: 6.274 ± 0.717
4.492GlySer: 4.492 ± 0.712
5.49GlyThr: 5.49 ± 0.605
6.274GlyVal: 6.274 ± 0.726
2.139GlyTrp: 2.139 ± 0.364
1.64GlyTyr: 1.64 ± 0.256
0.0GlyXaa: 0.0 ± 0.0
His
2.424HisAla: 2.424 ± 0.49
0.356HisCys: 0.356 ± 0.139
1.426HisAsp: 1.426 ± 0.355
1.212HisGlu: 1.212 ± 0.28
0.499HisPhe: 0.499 ± 0.182
1.854HisGly: 1.854 ± 0.396
0.998HisHis: 0.998 ± 0.309
1.782HisIle: 1.782 ± 0.418
0.57HisLys: 0.57 ± 0.189
2.424HisLeu: 2.424 ± 0.538
0.285HisMet: 0.285 ± 0.162
0.356HisAsn: 0.356 ± 0.15
1.569HisPro: 1.569 ± 0.335
1.069HisGln: 1.069 ± 0.303
1.212HisArg: 1.212 ± 0.274
0.998HisSer: 0.998 ± 0.272
1.426HisThr: 1.426 ± 0.304
2.638HisVal: 2.638 ± 0.507
0.57HisTrp: 0.57 ± 0.252
0.57HisTyr: 0.57 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
7.272IleAla: 7.272 ± 0.636
0.214IleCys: 0.214 ± 0.147
4.706IleAsp: 4.706 ± 0.728
2.424IleGlu: 2.424 ± 0.458
1.069IlePhe: 1.069 ± 0.253
4.492IleGly: 4.492 ± 0.873
1.497IleHis: 1.497 ± 0.416
2.567IleIle: 2.567 ± 0.379
1.069IleLys: 1.069 ± 0.286
2.781IleLeu: 2.781 ± 0.512
0.57IleMet: 0.57 ± 0.154
1.283IleAsn: 1.283 ± 0.245
2.923IlePro: 2.923 ± 0.538
1.64IleGln: 1.64 ± 0.294
3.066IleArg: 3.066 ± 0.545
2.495IleSer: 2.495 ± 0.503
3.921IleThr: 3.921 ± 0.548
3.422IleVal: 3.422 ± 0.45
0.285IleTrp: 0.285 ± 0.2
0.998IleTyr: 0.998 ± 0.346
0.0IleXaa: 0.0 ± 0.0
Lys
4.492LysAla: 4.492 ± 0.782
0.0LysCys: 0.0 ± 0.0
1.925LysAsp: 1.925 ± 0.356
1.212LysGlu: 1.212 ± 0.295
0.927LysPhe: 0.927 ± 0.323
2.139LysGly: 2.139 ± 0.537
0.784LysHis: 0.784 ± 0.272
1.497LysIle: 1.497 ± 0.328
1.141LysLys: 1.141 ± 0.308
3.636LysLeu: 3.636 ± 0.546
0.927LysMet: 0.927 ± 0.265
0.499LysAsn: 0.499 ± 0.174
1.996LysPro: 1.996 ± 0.374
1.925LysGln: 1.925 ± 0.388
1.782LysArg: 1.782 ± 0.362
2.068LysSer: 2.068 ± 0.432
2.424LysThr: 2.424 ± 0.415
1.64LysVal: 1.64 ± 0.315
0.998LysTrp: 0.998 ± 0.24
0.998LysTyr: 0.998 ± 0.192
0.0LysXaa: 0.0 ± 0.0
Leu
9.981LeuAla: 9.981 ± 0.769
0.428LeuCys: 0.428 ± 0.178
6.345LeuAsp: 6.345 ± 0.935
3.707LeuGlu: 3.707 ± 0.42
1.497LeuPhe: 1.497 ± 0.355
6.844LeuGly: 6.844 ± 0.648
1.925LeuHis: 1.925 ± 0.368
3.779LeuIle: 3.779 ± 0.458
2.495LeuLys: 2.495 ± 0.463
4.42LeuLeu: 4.42 ± 0.568
1.569LeuMet: 1.569 ± 0.291
1.925LeuAsn: 1.925 ± 0.352
5.561LeuPro: 5.561 ± 0.724
2.21LeuGln: 2.21 ± 0.413
6.702LeuArg: 6.702 ± 0.745
4.349LeuSer: 4.349 ± 0.543
5.276LeuThr: 5.276 ± 0.677
4.777LeuVal: 4.777 ± 0.463
1.64LeuTrp: 1.64 ± 0.378
1.782LeuTyr: 1.782 ± 0.404
0.0LeuXaa: 0.0 ± 0.0
Met
2.923MetAla: 2.923 ± 0.534
0.071MetCys: 0.071 ± 0.071
1.497MetAsp: 1.497 ± 0.319
1.569MetGlu: 1.569 ± 0.411
0.285MetPhe: 0.285 ± 0.142
2.139MetGly: 2.139 ± 0.613
0.356MetHis: 0.356 ± 0.151
0.713MetIle: 0.713 ± 0.27
1.069MetLys: 1.069 ± 0.251
1.069MetLeu: 1.069 ± 0.271
0.356MetMet: 0.356 ± 0.14
0.784MetAsn: 0.784 ± 0.211
1.854MetPro: 1.854 ± 0.33
0.784MetGln: 0.784 ± 0.215
1.854MetArg: 1.854 ± 0.546
1.711MetSer: 1.711 ± 0.313
3.707MetThr: 3.707 ± 0.659
1.141MetVal: 1.141 ± 0.235
0.285MetTrp: 0.285 ± 0.184
0.285MetTyr: 0.285 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
3.636AsnAla: 3.636 ± 0.426
0.143AsnCys: 0.143 ± 0.103
1.141AsnAsp: 1.141 ± 0.27
0.856AsnGlu: 0.856 ± 0.223
0.499AsnPhe: 0.499 ± 0.213
2.852AsnGly: 2.852 ± 0.592
0.642AsnHis: 0.642 ± 0.225
1.212AsnIle: 1.212 ± 0.224
0.642AsnLys: 0.642 ± 0.23
2.709AsnLeu: 2.709 ± 0.469
0.428AsnMet: 0.428 ± 0.165
0.428AsnAsn: 0.428 ± 0.166
2.21AsnPro: 2.21 ± 0.326
0.499AsnGln: 0.499 ± 0.208
1.996AsnArg: 1.996 ± 0.344
0.856AsnSer: 0.856 ± 0.225
1.283AsnThr: 1.283 ± 0.24
1.569AsnVal: 1.569 ± 0.316
0.143AsnTrp: 0.143 ± 0.095
0.642AsnTyr: 0.642 ± 0.207
0.0AsnXaa: 0.0 ± 0.0
Pro
7.058ProAla: 7.058 ± 0.69
0.285ProCys: 0.285 ± 0.139
5.419ProAsp: 5.419 ± 0.66
4.634ProGlu: 4.634 ± 0.621
1.569ProPhe: 1.569 ± 0.231
5.49ProGly: 5.49 ± 0.794
0.927ProHis: 0.927 ± 0.275
2.495ProIle: 2.495 ± 0.421
2.21ProLys: 2.21 ± 0.497
3.636ProLeu: 3.636 ± 0.488
1.782ProMet: 1.782 ± 0.341
1.283ProAsn: 1.283 ± 0.252
3.28ProPro: 3.28 ± 0.762
1.283ProGln: 1.283 ± 0.284
3.494ProArg: 3.494 ± 0.613
2.353ProSer: 2.353 ± 0.487
4.064ProThr: 4.064 ± 0.545
4.42ProVal: 4.42 ± 0.502
1.497ProTrp: 1.497 ± 0.388
0.713ProTyr: 0.713 ± 0.241
0.0ProXaa: 0.0 ± 0.0
Gln
5.205GlnAla: 5.205 ± 0.588
0.143GlnCys: 0.143 ± 0.094
1.069GlnAsp: 1.069 ± 0.32
1.854GlnGlu: 1.854 ± 0.365
0.713GlnPhe: 0.713 ± 0.334
2.638GlnGly: 2.638 ± 0.438
0.642GlnHis: 0.642 ± 0.217
1.141GlnIle: 1.141 ± 0.255
1.426GlnLys: 1.426 ± 0.279
3.707GlnLeu: 3.707 ± 0.396
1.141GlnMet: 1.141 ± 0.252
0.713GlnAsn: 0.713 ± 0.271
1.426GlnPro: 1.426 ± 0.272
1.497GlnGln: 1.497 ± 0.3
2.424GlnArg: 2.424 ± 0.388
1.069GlnSer: 1.069 ± 0.268
1.854GlnThr: 1.854 ± 0.357
3.707GlnVal: 3.707 ± 0.455
1.212GlnTrp: 1.212 ± 0.281
0.856GlnTyr: 0.856 ± 0.259
0.0GlnXaa: 0.0 ± 0.0
Arg
8.128ArgAla: 8.128 ± 0.685
0.642ArgCys: 0.642 ± 0.206
6.559ArgAsp: 6.559 ± 0.969
4.135ArgGlu: 4.135 ± 0.488
1.854ArgPhe: 1.854 ± 0.287
4.706ArgGly: 4.706 ± 0.692
1.497ArgHis: 1.497 ± 0.333
3.779ArgIle: 3.779 ± 0.559
2.781ArgLys: 2.781 ± 0.347
5.49ArgLeu: 5.49 ± 0.919
2.638ArgMet: 2.638 ± 0.37
1.782ArgAsn: 1.782 ± 0.352
3.422ArgPro: 3.422 ± 0.731
2.068ArgGln: 2.068 ± 0.501
6.844ArgArg: 6.844 ± 0.976
2.994ArgSer: 2.994 ± 0.441
3.993ArgThr: 3.993 ± 0.402
4.492ArgVal: 4.492 ± 0.549
2.068ArgTrp: 2.068 ± 0.426
1.283ArgTyr: 1.283 ± 0.383
0.0ArgXaa: 0.0 ± 0.0
Ser
5.49SerAla: 5.49 ± 0.805
0.428SerCys: 0.428 ± 0.195
2.567SerAsp: 2.567 ± 0.488
2.495SerGlu: 2.495 ± 0.514
1.497SerPhe: 1.497 ± 0.334
4.848SerGly: 4.848 ± 1.0
1.283SerHis: 1.283 ± 0.449
1.782SerIle: 1.782 ± 0.375
1.283SerLys: 1.283 ± 0.305
3.636SerLeu: 3.636 ± 0.661
1.141SerMet: 1.141 ± 0.299
0.998SerAsn: 0.998 ± 0.243
2.424SerPro: 2.424 ± 0.43
1.64SerGln: 1.64 ± 0.319
3.779SerArg: 3.779 ± 0.569
2.424SerSer: 2.424 ± 0.508
3.921SerThr: 3.921 ± 0.654
3.636SerVal: 3.636 ± 0.534
1.069SerTrp: 1.069 ± 0.233
1.141SerTyr: 1.141 ± 0.303
0.0SerXaa: 0.0 ± 0.0
Thr
8.413ThrAla: 8.413 ± 0.722
0.285ThrCys: 0.285 ± 0.157
4.064ThrAsp: 4.064 ± 0.744
3.779ThrGlu: 3.779 ± 0.414
1.355ThrPhe: 1.355 ± 0.349
5.918ThrGly: 5.918 ± 0.499
1.355ThrHis: 1.355 ± 0.296
4.206ThrIle: 4.206 ± 0.776
2.21ThrLys: 2.21 ± 0.294
5.561ThrLeu: 5.561 ± 0.624
1.996ThrMet: 1.996 ± 0.387
2.068ThrAsn: 2.068 ± 0.351
4.634ThrPro: 4.634 ± 0.74
1.64ThrGln: 1.64 ± 0.387
4.064ThrArg: 4.064 ± 0.723
2.638ThrSer: 2.638 ± 0.647
5.775ThrThr: 5.775 ± 0.794
4.991ThrVal: 4.991 ± 0.864
1.283ThrTrp: 1.283 ± 0.319
1.711ThrTyr: 1.711 ± 0.373
0.0ThrXaa: 0.0 ± 0.0
Val
10.338ValAla: 10.338 ± 0.952
0.428ValCys: 0.428 ± 0.17
5.205ValAsp: 5.205 ± 0.658
5.347ValGlu: 5.347 ± 0.586
1.497ValPhe: 1.497 ± 0.319
5.49ValGly: 5.49 ± 0.796
1.426ValHis: 1.426 ± 0.387
3.137ValIle: 3.137 ± 0.504
2.139ValLys: 2.139 ± 0.437
4.777ValLeu: 4.777 ± 0.69
1.141ValMet: 1.141 ± 0.476
1.782ValAsn: 1.782 ± 0.429
3.208ValPro: 3.208 ± 0.505
1.782ValGln: 1.782 ± 0.363
4.919ValArg: 4.919 ± 0.646
3.921ValSer: 3.921 ± 0.534
4.848ValThr: 4.848 ± 0.574
4.278ValVal: 4.278 ± 0.673
1.854ValTrp: 1.854 ± 0.296
1.283ValTyr: 1.283 ± 0.259
0.0ValXaa: 0.0 ± 0.0
Trp
3.28TrpAla: 3.28 ± 0.524
0.356TrpCys: 0.356 ± 0.161
1.569TrpAsp: 1.569 ± 0.378
1.355TrpGlu: 1.355 ± 0.327
0.713TrpPhe: 0.713 ± 0.224
1.283TrpGly: 1.283 ± 0.332
0.499TrpHis: 0.499 ± 0.188
1.069TrpIle: 1.069 ± 0.347
0.428TrpLys: 0.428 ± 0.238
1.996TrpLeu: 1.996 ± 0.393
0.642TrpMet: 0.642 ± 0.21
0.713TrpAsn: 0.713 ± 0.212
1.426TrpPro: 1.426 ± 0.327
0.784TrpGln: 0.784 ± 0.193
2.139TrpArg: 2.139 ± 0.286
1.069TrpSer: 1.069 ± 0.294
1.069TrpThr: 1.069 ± 0.329
1.355TrpVal: 1.355 ± 0.335
0.428TrpTrp: 0.428 ± 0.15
0.143TrpTyr: 0.143 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.567TyrAla: 2.567 ± 0.337
0.285TyrCys: 0.285 ± 0.174
1.497TyrAsp: 1.497 ± 0.271
1.497TyrGlu: 1.497 ± 0.288
0.428TyrPhe: 0.428 ± 0.171
1.64TyrGly: 1.64 ± 0.334
0.784TyrHis: 0.784 ± 0.263
1.141TyrIle: 1.141 ± 0.301
0.499TyrLys: 0.499 ± 0.248
1.426TyrLeu: 1.426 ± 0.378
0.214TyrMet: 0.214 ± 0.13
0.499TyrAsn: 0.499 ± 0.185
1.069TyrPro: 1.069 ± 0.296
0.856TyrGln: 0.856 ± 0.275
2.139TyrArg: 2.139 ± 0.404
1.355TyrSer: 1.355 ± 0.415
1.64TyrThr: 1.64 ± 0.308
1.64TyrVal: 1.64 ± 0.286
0.285TyrTrp: 0.285 ± 0.172
0.143TyrTyr: 0.143 ± 0.095
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (14027 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski