Arthroderma otae (strain ATCC MYA-4605 / CBS 113480) (Microsporum canis)
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8764 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C5FZM4|C5FZM4_ARTOC Uncharacterized protein OS=Arthroderma otae (strain ATCC MYA-4605 / CBS 113480) OX=554155 GN=MCYG_08146 PE=4 SV=1
MM1 pKa = 7.42 SRR3 pKa = 11.84 PTNEE7 pKa = 3.42 VTIQNSALLIAYY19 pKa = 7.5 EE20 pKa = 4.42 IIATKK25 pKa = 10.08 GAYY28 pKa = 8.5 IHH30 pKa = 6.43 HH31 pKa = 5.51 TVKK34 pKa = 10.3 MFIVQILLDD43 pKa = 3.55 IAVWLLGFTSALVPEE58 pKa = 4.75 EE59 pKa = 4.25 AAHH62 pKa = 6.22 EE63 pKa = 4.09 GHH65 pKa = 7.16 PPTEE69 pKa = 3.97 RR70 pKa = 11.84 AEE72 pKa = 4.14 MSDD75 pKa = 3.25 APNFEE80 pKa = 4.41 HH81 pKa = 7.16 PLPYY85 pKa = 10.37 EE86 pKa = 4.18 GAEE89 pKa = 4.11 EE90 pKa = 4.45 DD91 pKa = 4.78 LEE93 pKa = 5.69 PDD95 pKa = 3.44 TPEE98 pKa = 3.64 NMTWIMDD105 pKa = 4.14 YY106 pKa = 10.44 PSHH109 pKa = 6.92 PPLYY113 pKa = 9.66 FSSSTSSQSSSSQEE127 pKa = 3.82 SYY129 pKa = 11.55 LEE131 pKa = 4.51 IISSLEE137 pKa = 3.84 ALWDD141 pKa = 3.72 DD142 pKa = 4.02 WDD144 pKa = 4.21 PQPEE148 pKa = 4.38 EE149 pKa = 3.7 IDD151 pKa = 3.84 YY152 pKa = 10.39 PDD154 pKa = 4.43 RR155 pKa = 11.84 PTTPGILEE163 pKa = 4.04 TTLANDD169 pKa = 3.49 TGSLFSRR176 pKa = 11.84 SDD178 pKa = 3.0 TDD180 pKa = 4.0 AEE182 pKa = 4.61 SSQPMSPRR190 pKa = 11.84 PATLDD195 pKa = 3.16 SEE197 pKa = 5.25 YY198 pKa = 11.15 YY199 pKa = 10.12 IFNPTGDD206 pKa = 4.75 LEE208 pKa = 4.32 MSDD211 pKa = 3.48 TGLL214 pKa = 3.71
Molecular weight: 23.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.91
IPC_protein 3.872
Toseland 3.694
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.732
Rodwell 3.706
Grimsley 3.605
Solomon 3.834
Lehninger 3.783
Nozaki 3.961
DTASelect 4.113
Thurlkill 3.719
EMBOSS 3.745
Sillero 3.986
Patrickios 1.125
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.89
Protein with the highest isoelectric point:
>tr|C5FVX6|C5FVX6_ARTOC Alpha/beta hydrolase fold-3 domain-containing protein OS=Arthroderma otae (strain ATCC MYA-4605 / CBS 113480) OX=554155 GN=MCYG_06879 PE=4 SV=1
MM1 pKa = 7.46 PRR3 pKa = 11.84 ISPSQSVSQSVSQSVWEE20 pKa = 4.39 EE21 pKa = 3.79 GSLPQAVPSSCCRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GLVSGDD43 pKa = 3.12 MGRR46 pKa = 11.84 NEE48 pKa = 4.84 LKK50 pKa = 10.62 LPPSRR55 pKa = 11.84 FIVFSLAHH63 pKa = 6.12 LRR65 pKa = 11.84 WAAYY69 pKa = 9.96 CFIKK73 pKa = 10.84 GMLEE77 pKa = 3.71 LVTYY81 pKa = 10.75 VRR83 pKa = 11.84 DD84 pKa = 3.6 QQNNNISHH92 pKa = 6.04 NRR94 pKa = 11.84 VNKK97 pKa = 10.41 NKK99 pKa = 8.95 LTFSRR104 pKa = 4.33
Molecular weight: 11.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.619
IPC_protein 10.526
Toseland 10.716
ProMoST 10.891
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.935
Grimsley 10.847
Solomon 10.965
Lehninger 10.935
Nozaki 10.73
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.716
IPC_peptide 10.965
IPC2_peptide 9.867
IPC2.peptide.svr19 8.755
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8764
0
8764
4258598
51
7914
485.9
53.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.042 ± 0.025
1.277 ± 0.009
5.61 ± 0.016
6.229 ± 0.024
3.648 ± 0.017
6.644 ± 0.022
2.42 ± 0.011
5.258 ± 0.02
5.101 ± 0.021
8.938 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.133 ± 0.009
3.837 ± 0.013
6.144 ± 0.029
4.045 ± 0.018
6.155 ± 0.02
8.667 ± 0.028
5.877 ± 0.016
5.838 ± 0.019
1.333 ± 0.008
2.804 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here