Legionella jordanis
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2787 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W0VAU6|A0A0W0VAU6_9GAMM Putative stress-induced protein OS=Legionella jordanis OX=456 GN=Ljor_1051 PE=4 SV=1
MM1 pKa = 7.94 KK2 pKa = 10.24 IMQEE6 pKa = 3.98 YY7 pKa = 9.8 KK8 pKa = 10.33 RR9 pKa = 11.84 YY10 pKa = 9.34 ICVICGFIYY19 pKa = 10.62 DD20 pKa = 4.34 EE21 pKa = 4.7 AEE23 pKa = 3.78 GWPEE27 pKa = 4.22 DD28 pKa = 4.13 GIEE31 pKa = 4.38 PGTRR35 pKa = 11.84 WEE37 pKa = 4.62 DD38 pKa = 3.5 VPEE41 pKa = 3.85 NWFCPDD47 pKa = 3.39 CGAGKK52 pKa = 10.44 EE53 pKa = 3.98 DD54 pKa = 4.11 FEE56 pKa = 4.49 MVEE59 pKa = 3.99 MDD61 pKa = 3.36
Molecular weight: 7.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.79
IPC2_protein 3.973
IPC_protein 3.846
Toseland 3.681
ProMoST 3.91
Dawson 3.821
Bjellqvist 4.037
Wikipedia 3.719
Rodwell 3.694
Grimsley 3.592
Solomon 3.795
Lehninger 3.757
Nozaki 3.948
DTASelect 4.075
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.961
Patrickios 0.769
IPC_peptide 3.795
IPC2_peptide 3.948
IPC2.peptide.svr19 3.929
Protein with the highest isoelectric point:
>tr|A0A0W0VG73|A0A0W0VG73_9GAMM Uncharacterized protein OS=Legionella jordanis OX=456 GN=Ljor_0048 PE=4 SV=1
AA1 pKa = 7.15 QQRR4 pKa = 11.84 AQQQAQQRR12 pKa = 11.84 AQQQAQQKK20 pKa = 9.25 AQQQAQQRR28 pKa = 11.84 AQQQAQQQAQQRR40 pKa = 11.84 AQQRR44 pKa = 11.84 AQQQAQQQAQRR55 pKa = 11.84 AAAVRR60 pKa = 11.84 SAHH63 pKa = 5.28 QAASVRR69 pKa = 11.84 AAQQQAQQRR78 pKa = 11.84 AQQTAAQRR86 pKa = 11.84 AYY88 pKa = 10.66 RR89 pKa = 11.84 RR90 pKa = 3.74
Molecular weight: 10.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.439
IPC2_protein 11.14
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.237
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.974
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.129
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2787
0
2787
923784
50
3921
331.5
37.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.112 ± 0.049
1.213 ± 0.018
4.641 ± 0.034
6.181 ± 0.051
4.619 ± 0.033
6.017 ± 0.044
2.556 ± 0.022
6.961 ± 0.039
5.96 ± 0.042
11.437 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.337 ± 0.018
4.669 ± 0.034
4.161 ± 0.029
4.881 ± 0.041
4.561 ± 0.034
6.654 ± 0.029
4.705 ± 0.03
5.824 ± 0.039
1.19 ± 0.016
3.319 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here