Flavobacterium aurantiibacter
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2902 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A255ZQK0|A0A255ZQK0_9FLAO Uncharacterized protein OS=Flavobacterium aurantiibacter OX=2023067 GN=CHX27_09375 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 10.36 KK3 pKa = 9.42 IQLFFLALSTALTLSGCEE21 pKa = 3.99 TDD23 pKa = 5.45 GGDD26 pKa = 4.15 SKK28 pKa = 11.84 LNLIEE33 pKa = 4.66 AAVPDD38 pKa = 3.56 ITINDD43 pKa = 3.76 TSDD46 pKa = 3.43 EE47 pKa = 4.52 IINLVAVTNGGDD59 pKa = 3.31 INLSLTLDD67 pKa = 3.31 KK68 pKa = 11.55 GFGKK72 pKa = 10.27 VSSMDD77 pKa = 3.25 VVLFYY82 pKa = 11.11 YY83 pKa = 10.58 KK84 pKa = 10.38 SDD86 pKa = 3.06 GSVFRR91 pKa = 11.84 SVLQSGITEE100 pKa = 4.6 FPATVSITKK109 pKa = 10.45 NSIFDD114 pKa = 3.42 AFEE117 pKa = 4.24 QINVPADD124 pKa = 3.59 FEE126 pKa = 4.71 VGDD129 pKa = 4.34 EE130 pKa = 4.23 LLITADD136 pKa = 3.43 LTLPNGTVIKK146 pKa = 9.75 MYY148 pKa = 10.85 NDD150 pKa = 3.71 DD151 pKa = 3.63 GSRR154 pKa = 11.84 NFGANVANSAVYY166 pKa = 10.21 SVSQSFLVSCPSDD179 pKa = 3.15 LGGTYY184 pKa = 10.33 NYY186 pKa = 8.33 STTDD190 pKa = 2.87 AYY192 pKa = 10.42 EE193 pKa = 4.1 PGGASVAGPITGTVTFTAIGGGAYY217 pKa = 9.21 EE218 pKa = 4.54 ISDD221 pKa = 3.38 ASFGGWEE228 pKa = 4.42 AIYY231 pKa = 10.6 GPGNISEE238 pKa = 4.57 GVTLVDD244 pKa = 3.2 VCNTIQFGGNDD255 pKa = 3.74 QFGDD259 pKa = 3.9 TYY261 pKa = 11.32 TMSNLVVNGASLSFSWTNTYY281 pKa = 11.16 GEE283 pKa = 4.55 GGEE286 pKa = 4.4 VTLTRR291 pKa = 11.84 PDD293 pKa = 3.57 GSPWPPLSLL302 pKa = 4.52
Molecular weight: 31.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.49
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.656
Rodwell 3.528
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.062
Thurlkill 3.541
EMBOSS 3.656
Sillero 3.834
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A255ZQJ0|A0A255ZQJ0_9FLAO Translation initiation factor SUI1-related protein OS=Flavobacterium aurantiibacter OX=2023067 GN=CHX27_08580 PE=3 SV=1
MM1 pKa = 7.65 HH2 pKa = 7.53 IFAQGVAAEE11 pKa = 4.43 SRR13 pKa = 11.84 AEE15 pKa = 4.05 RR16 pKa = 11.84 KK17 pKa = 8.8 PVQPAAVRR25 pKa = 11.84 RR26 pKa = 11.84 PTEE29 pKa = 3.86 AVPQFGGTVLEE40 pKa = 4.56 RR41 pKa = 11.84 CALQRR46 pKa = 11.84 KK47 pKa = 5.44 TRR49 pKa = 11.84 PAALLYY55 pKa = 9.86 FHH57 pKa = 7.45 EE58 pKa = 5.08 IFAAGCAQKK67 pKa = 9.74 TGIDD71 pKa = 3.33 TFTRR75 pKa = 11.84 QITLLVCVPLLKK87 pKa = 10.25 ILAHH91 pKa = 5.35 VRR93 pKa = 11.84 RR94 pKa = 4.37
Molecular weight: 10.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.487
IPC_protein 10.16
Toseland 10.716
ProMoST 10.862
Dawson 10.774
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.965
Grimsley 10.804
Solomon 10.935
Lehninger 10.906
Nozaki 10.73
DTASelect 10.482
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.76
IPC_peptide 10.935
IPC2_peptide 9.809
IPC2.peptide.svr19 8.811
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2902
0
2902
924509
34
3242
318.6
35.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.558 ± 0.056
0.851 ± 0.017
5.197 ± 0.033
6.162 ± 0.057
5.572 ± 0.039
6.245 ± 0.049
1.668 ± 0.023
7.201 ± 0.043
6.954 ± 0.076
9.468 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.921 ± 0.021
5.804 ± 0.05
3.577 ± 0.033
3.825 ± 0.026
3.933 ± 0.032
6.544 ± 0.044
6.092 ± 0.073
6.576 ± 0.041
0.979 ± 0.017
3.868 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here