Comamonadaceae bacterium NML161473

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; unclassified Comamonadaceae

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2324 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M6PZJ4|A0A3M6PZJ4_9BURK CoA pyrophosphatase OS=Comamonadaceae bacterium NML161473 OX=2478744 GN=EBQ25_11030 PE=4 SV=1
MM1 pKa = 6.39TTEE4 pKa = 4.02ATTEE8 pKa = 4.15VQATPDD14 pKa = 3.39MPINFTDD21 pKa = 3.41AAAAKK26 pKa = 9.92VADD29 pKa = 5.47LIAEE33 pKa = 4.31EE34 pKa = 4.82GNPDD38 pKa = 2.99LKK40 pKa = 11.13LRR42 pKa = 11.84VFVQGGGCSGFQYY55 pKa = 10.94GFTFDD60 pKa = 4.81EE61 pKa = 4.64ITNEE65 pKa = 4.77DD66 pKa = 4.2DD67 pKa = 3.25TTMTKK72 pKa = 10.66NGVSLLIDD80 pKa = 3.63PMSYY84 pKa = 10.35QYY86 pKa = 11.66LFGAEE91 pKa = 3.65IDD93 pKa = 4.05YY94 pKa = 11.34KK95 pKa = 11.14EE96 pKa = 4.67DD97 pKa = 3.23LQGAQFVIKK106 pKa = 10.45NPNAEE111 pKa = 4.34STCGCGSSFSVV122 pKa = 3.54

Molecular weight: 13.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M6QBC2|A0A3M6QBC2_9BURK ABC transporter substrate-binding protein OS=Comamonadaceae bacterium NML161473 OX=2478744 GN=EBQ25_05095 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.44GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LGLAA45 pKa = 3.49

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2324

0

2324

793159

35

2232

341.3

37.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.474 ± 0.082

0.935 ± 0.014

4.844 ± 0.037

5.196 ± 0.044

3.31 ± 0.029

7.913 ± 0.05

2.588 ± 0.029

4.39 ± 0.039

3.129 ± 0.054

10.986 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.025

2.526 ± 0.029

5.559 ± 0.041

5.4 ± 0.05

6.727 ± 0.046

5.174 ± 0.035

4.76 ± 0.04

6.796 ± 0.042

1.616 ± 0.025

2.275 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski