Amino acid dipepetide frequency for Comamonadaceae bacterium NML161473

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.873AlaAla: 18.873 ± 0.282
1.498AlaCys: 1.498 ± 0.051
6.093AlaAsp: 6.093 ± 0.103
6.406AlaGlu: 6.406 ± 0.117
3.968AlaPhe: 3.968 ± 0.071
9.576AlaGly: 9.576 ± 0.132
3.437AlaHis: 3.437 ± 0.074
5.636AlaIle: 5.636 ± 0.101
3.805AlaLys: 3.805 ± 0.106
17.391AlaLeu: 17.391 ± 0.253
3.315AlaMet: 3.315 ± 0.066
2.858AlaAsn: 2.858 ± 0.074
7.514AlaPro: 7.514 ± 0.134
9.312AlaGln: 9.312 ± 0.152
9.108AlaArg: 9.108 ± 0.14
7.323AlaSer: 7.323 ± 0.102
5.83AlaThr: 5.83 ± 0.102
8.143AlaVal: 8.143 ± 0.122
2.104AlaTrp: 2.104 ± 0.06
2.551AlaTyr: 2.551 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
1.248CysAla: 1.248 ± 0.042
0.119CysCys: 0.119 ± 0.014
0.557CysAsp: 0.557 ± 0.03
0.456CysGlu: 0.456 ± 0.022
0.294CysPhe: 0.294 ± 0.021
0.908CysGly: 0.908 ± 0.041
0.257CysHis: 0.257 ± 0.021
0.415CysIle: 0.415 ± 0.024
0.211CysLys: 0.211 ± 0.017
0.875CysLeu: 0.875 ± 0.034
0.265CysMet: 0.265 ± 0.019
0.214CysAsn: 0.214 ± 0.016
0.559CysPro: 0.559 ± 0.033
0.408CysGln: 0.408 ± 0.023
0.511CysArg: 0.511 ± 0.026
0.508CysSer: 0.508 ± 0.026
0.556CysThr: 0.556 ± 0.036
0.676CysVal: 0.676 ± 0.033
0.127CysTrp: 0.127 ± 0.013
0.192CysTyr: 0.192 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
7.541AspAla: 7.541 ± 0.106
0.415AspCys: 0.415 ± 0.022
2.244AspAsp: 2.244 ± 0.054
2.975AspGlu: 2.975 ± 0.068
2.097AspPhe: 2.097 ± 0.053
4.096AspGly: 4.096 ± 0.082
0.92AspHis: 0.92 ± 0.031
2.332AspIle: 2.332 ± 0.057
1.653AspLys: 1.653 ± 0.063
4.761AspLeu: 4.761 ± 0.082
1.231AspMet: 1.231 ± 0.037
1.122AspAsn: 1.122 ± 0.039
2.372AspPro: 2.372 ± 0.055
1.485AspGln: 1.485 ± 0.039
2.524AspArg: 2.524 ± 0.057
2.247AspSer: 2.247 ± 0.062
2.185AspThr: 2.185 ± 0.055
3.774AspVal: 3.774 ± 0.08
1.087AspTrp: 1.087 ± 0.036
1.383AspTyr: 1.383 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
6.736GluAla: 6.736 ± 0.117
0.354GluCys: 0.354 ± 0.024
2.344GluAsp: 2.344 ± 0.064
2.562GluGlu: 2.562 ± 0.07
1.582GluPhe: 1.582 ± 0.048
3.83GluGly: 3.83 ± 0.078
1.478GluHis: 1.478 ± 0.051
2.698GluIle: 2.698 ± 0.064
2.127GluLys: 2.127 ± 0.063
5.401GluLeu: 5.401 ± 0.101
1.246GluMet: 1.246 ± 0.046
1.333GluAsn: 1.333 ± 0.049
2.456GluPro: 2.456 ± 0.06
3.434GluGln: 3.434 ± 0.1
4.588GluArg: 4.588 ± 0.082
2.248GluSer: 2.248 ± 0.055
2.214GluThr: 2.214 ± 0.059
3.577GluVal: 3.577 ± 0.082
0.682GluTrp: 0.682 ± 0.032
1.073GluTyr: 1.073 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
4.215PheAla: 4.215 ± 0.07
0.358PheCys: 0.358 ± 0.02
2.22PheAsp: 2.22 ± 0.055
1.954PheGlu: 1.954 ± 0.052
1.377PhePhe: 1.377 ± 0.053
2.986PheGly: 2.986 ± 0.068
0.76PheHis: 0.76 ± 0.035
1.518PheIle: 1.518 ± 0.045
1.093PheLys: 1.093 ± 0.042
2.982PheLeu: 2.982 ± 0.071
0.804PheMet: 0.804 ± 0.031
1.058PheAsn: 1.058 ± 0.039
1.376PhePro: 1.376 ± 0.04
1.185PheGln: 1.185 ± 0.038
1.599PheArg: 1.599 ± 0.045
2.065PheSer: 2.065 ± 0.048
1.814PheThr: 1.814 ± 0.048
2.32PheVal: 2.32 ± 0.051
0.548PheTrp: 0.548 ± 0.029
0.867PheTyr: 0.867 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
8.929GlyAla: 8.929 ± 0.11
0.867GlyCys: 0.867 ± 0.036
3.666GlyAsp: 3.666 ± 0.08
4.11GlyGlu: 4.11 ± 0.078
2.967GlyPhe: 2.967 ± 0.072
6.245GlyGly: 6.245 ± 0.119
2.118GlyHis: 2.118 ± 0.057
3.649GlyIle: 3.649 ± 0.074
3.302GlyLys: 3.302 ± 0.081
8.726GlyLeu: 8.726 ± 0.144
2.331GlyMet: 2.331 ± 0.053
1.962GlyAsn: 1.962 ± 0.063
2.928GlyPro: 2.928 ± 0.07
4.937GlyGln: 4.937 ± 0.091
5.106GlyArg: 5.106 ± 0.091
4.287GlySer: 4.287 ± 0.07
3.388GlyThr: 3.388 ± 0.083
5.906GlyVal: 5.906 ± 0.103
1.508GlyTrp: 1.508 ± 0.048
2.201GlyTyr: 2.201 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
3.565HisAla: 3.565 ± 0.08
0.339HisCys: 0.339 ± 0.02
1.186HisAsp: 1.186 ± 0.042
1.272HisGlu: 1.272 ± 0.041
0.968HisPhe: 0.968 ± 0.034
2.169HisGly: 2.169 ± 0.064
0.659HisHis: 0.659 ± 0.034
1.398HisIle: 1.398 ± 0.044
0.678HisLys: 0.678 ± 0.033
2.568HisLeu: 2.568 ± 0.06
0.604HisMet: 0.604 ± 0.027
0.68HisAsn: 0.68 ± 0.028
1.606HisPro: 1.606 ± 0.042
1.025HisGln: 1.025 ± 0.043
1.405HisArg: 1.405 ± 0.045
1.283HisSer: 1.283 ± 0.043
1.503HisThr: 1.503 ± 0.055
1.648HisVal: 1.648 ± 0.047
0.569HisTrp: 0.569 ± 0.029
0.756HisTyr: 0.756 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.406IleAla: 6.406 ± 0.1
0.412IleCys: 0.412 ± 0.029
2.933IleAsp: 2.933 ± 0.067
3.175IleGlu: 3.175 ± 0.074
1.364IlePhe: 1.364 ± 0.05
3.912IleGly: 3.912 ± 0.079
1.039IleHis: 1.039 ± 0.039
1.597IleIle: 1.597 ± 0.056
1.48IleLys: 1.48 ± 0.053
3.436IleLeu: 3.436 ± 0.078
0.793IleMet: 0.793 ± 0.035
1.403IleAsn: 1.403 ± 0.05
2.031IlePro: 2.031 ± 0.055
1.495IleGln: 1.495 ± 0.047
2.551IleArg: 2.551 ± 0.063
2.412IleSer: 2.412 ± 0.059
2.546IleThr: 2.546 ± 0.07
3.342IleVal: 3.342 ± 0.072
0.498IleTrp: 0.498 ± 0.025
1.078IleTyr: 1.078 ± 0.038
0.0IleXaa: 0.0 ± 0.0
Lys
4.062LysAla: 4.062 ± 0.107
0.159LysCys: 0.159 ± 0.015
1.542LysAsp: 1.542 ± 0.052
1.647LysGlu: 1.647 ± 0.058
0.741LysPhe: 0.741 ± 0.035
2.528LysGly: 2.528 ± 0.066
0.658LysHis: 0.658 ± 0.029
1.452LysIle: 1.452 ± 0.055
1.423LysLys: 1.423 ± 0.058
3.107LysLeu: 3.107 ± 0.07
0.741LysMet: 0.741 ± 0.036
1.108LysAsn: 1.108 ± 0.037
2.022LysPro: 2.022 ± 0.061
1.304LysGln: 1.304 ± 0.045
2.145LysArg: 2.145 ± 0.064
1.623LysSer: 1.623 ± 0.056
1.892LysThr: 1.892 ± 0.067
2.185LysVal: 2.185 ± 0.065
0.324LysTrp: 0.324 ± 0.021
0.628LysTyr: 0.628 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
15.658LeuAla: 15.658 ± 0.206
1.131LeuCys: 1.131 ± 0.043
5.54LeuAsp: 5.54 ± 0.088
5.571LeuGlu: 5.571 ± 0.096
3.231LeuPhe: 3.231 ± 0.076
8.914LeuGly: 8.914 ± 0.128
3.209LeuHis: 3.209 ± 0.085
4.37LeuIle: 4.37 ± 0.081
3.253LeuLys: 3.253 ± 0.081
12.184LeuLeu: 12.184 ± 0.204
2.544LeuMet: 2.544 ± 0.068
2.641LeuAsn: 2.641 ± 0.05
6.705LeuPro: 6.705 ± 0.102
5.672LeuGln: 5.672 ± 0.095
8.034LeuArg: 8.034 ± 0.115
5.797LeuSer: 5.797 ± 0.095
4.915LeuThr: 4.915 ± 0.084
6.63LeuVal: 6.63 ± 0.094
1.624LeuTrp: 1.624 ± 0.052
2.24LeuTyr: 2.24 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
3.07MetAla: 3.07 ± 0.075
0.159MetCys: 0.159 ± 0.013
1.062MetAsp: 1.062 ± 0.037
1.067MetGlu: 1.067 ± 0.039
0.615MetPhe: 0.615 ± 0.027
1.962MetGly: 1.962 ± 0.05
0.664MetHis: 0.664 ± 0.029
0.82MetIle: 0.82 ± 0.036
0.798MetLys: 0.798 ± 0.035
2.774MetLeu: 2.774 ± 0.068
0.524MetMet: 0.524 ± 0.03
0.731MetAsn: 0.731 ± 0.033
1.655MetPro: 1.655 ± 0.048
1.51MetGln: 1.51 ± 0.043
1.81MetArg: 1.81 ± 0.043
1.315MetSer: 1.315 ± 0.048
1.263MetThr: 1.263 ± 0.038
1.601MetVal: 1.601 ± 0.048
0.211MetTrp: 0.211 ± 0.016
0.361MetTyr: 0.361 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.427AsnAla: 3.427 ± 0.074
0.211AsnCys: 0.211 ± 0.017
1.207AsnAsp: 1.207 ± 0.046
1.077AsnGlu: 1.077 ± 0.04
0.857AsnPhe: 0.857 ± 0.035
2.1AsnGly: 2.1 ± 0.062
0.593AsnHis: 0.593 ± 0.029
1.263AsnIle: 1.263 ± 0.041
0.797AsnLys: 0.797 ± 0.036
2.544AsnLeu: 2.544 ± 0.063
0.579AsnMet: 0.579 ± 0.027
0.721AsnAsn: 0.721 ± 0.041
1.933AsnPro: 1.933 ± 0.052
1.046AsnGln: 1.046 ± 0.04
1.576AsnArg: 1.576 ± 0.046
1.141AsnSer: 1.141 ± 0.044
1.422AsnThr: 1.422 ± 0.046
1.729AsnVal: 1.729 ± 0.054
0.414AsnTrp: 0.414 ± 0.026
0.629AsnTyr: 0.629 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
8.213ProAla: 8.213 ± 0.178
0.393ProCys: 0.393 ± 0.021
2.873ProAsp: 2.873 ± 0.057
3.5ProGlu: 3.5 ± 0.074
1.698ProPhe: 1.698 ± 0.05
4.539ProGly: 4.539 ± 0.094
1.494ProHis: 1.494 ± 0.047
2.07ProIle: 2.07 ± 0.061
1.442ProLys: 1.442 ± 0.049
5.744ProLeu: 5.744 ± 0.102
1.295ProMet: 1.295 ± 0.041
1.319ProAsn: 1.319 ± 0.042
3.4ProPro: 3.4 ± 0.105
3.036ProGln: 3.036 ± 0.078
2.904ProArg: 2.904 ± 0.065
3.021ProSer: 3.021 ± 0.073
2.591ProThr: 2.591 ± 0.061
3.934ProVal: 3.934 ± 0.078
0.918ProTrp: 0.918 ± 0.036
1.209ProTyr: 1.209 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
9.197GlnAla: 9.197 ± 0.172
0.403GlnCys: 0.403 ± 0.024
2.15GlnAsp: 2.15 ± 0.055
2.566GlnGlu: 2.566 ± 0.095
1.405GlnPhe: 1.405 ± 0.046
4.258GlnGly: 4.258 ± 0.09
1.382GlnHis: 1.382 ± 0.047
1.969GlnIle: 1.969 ± 0.044
1.459GlnLys: 1.459 ± 0.046
4.869GlnLeu: 4.869 ± 0.085
1.121GlnMet: 1.121 ± 0.039
1.111GlnAsn: 1.111 ± 0.036
3.397GlnPro: 3.397 ± 0.079
3.211GlnGln: 3.211 ± 0.09
4.646GlnArg: 4.646 ± 0.095
2.645GlnSer: 2.645 ± 0.062
2.605GlnThr: 2.605 ± 0.065
3.374GlnVal: 3.374 ± 0.074
1.26GlnTrp: 1.26 ± 0.043
0.976GlnTyr: 0.976 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
7.715ArgAla: 7.715 ± 0.115
0.548ArgCys: 0.548 ± 0.028
3.088ArgAsp: 3.088 ± 0.066
3.78ArgGlu: 3.78 ± 0.081
2.532ArgPhe: 2.532 ± 0.068
4.079ArgGly: 4.079 ± 0.076
2.02ArgHis: 2.02 ± 0.06
3.605ArgIle: 3.605 ± 0.07
2.152ArgLys: 2.152 ± 0.064
8.143ArgLeu: 8.143 ± 0.123
1.861ArgMet: 1.861 ± 0.059
1.76ArgAsn: 1.76 ± 0.044
3.294ArgPro: 3.294 ± 0.073
4.22ArgGln: 4.22 ± 0.09
4.587ArgArg: 4.587 ± 0.09
3.345ArgSer: 3.345 ± 0.067
2.939ArgThr: 2.939 ± 0.067
4.306ArgVal: 4.306 ± 0.077
1.299ArgTrp: 1.299 ± 0.041
2.003ArgTyr: 2.003 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
7.304SerAla: 7.304 ± 0.104
0.437SerCys: 0.437 ± 0.028
2.399SerAsp: 2.399 ± 0.059
2.395SerGlu: 2.395 ± 0.058
1.856SerPhe: 1.856 ± 0.043
5.072SerGly: 5.072 ± 0.085
1.328SerHis: 1.328 ± 0.041
2.326SerIle: 2.326 ± 0.054
1.451SerLys: 1.451 ± 0.052
5.51SerLeu: 5.51 ± 0.089
1.186SerMet: 1.186 ± 0.04
1.305SerAsn: 1.305 ± 0.045
3.031SerPro: 3.031 ± 0.069
2.234SerGln: 2.234 ± 0.059
3.149SerArg: 3.149 ± 0.059
2.906SerSer: 2.906 ± 0.08
2.447SerThr: 2.447 ± 0.057
3.408SerVal: 3.408 ± 0.065
0.724SerTrp: 0.724 ± 0.031
1.273SerTyr: 1.273 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
5.855ThrAla: 5.855 ± 0.115
0.421ThrCys: 0.421 ± 0.03
2.056ThrAsp: 2.056 ± 0.054
2.092ThrGlu: 2.092 ± 0.057
1.546ThrPhe: 1.546 ± 0.039
4.138ThrGly: 4.138 ± 0.088
1.227ThrHis: 1.227 ± 0.04
2.155ThrIle: 2.155 ± 0.061
1.08ThrLys: 1.08 ± 0.041
5.926ThrLeu: 5.926 ± 0.085
0.915ThrMet: 0.915 ± 0.033
1.152ThrAsn: 1.152 ± 0.042
3.813ThrPro: 3.813 ± 0.078
2.157ThrGln: 2.157 ± 0.058
3.031ThrArg: 3.031 ± 0.068
2.38ThrSer: 2.38 ± 0.06
2.438ThrThr: 2.438 ± 0.069
3.663ThrVal: 3.663 ± 0.094
0.599ThrTrp: 0.599 ± 0.028
0.959ThrTyr: 0.959 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
8.283ValAla: 8.283 ± 0.127
0.74ValCys: 0.74 ± 0.036
3.427ValAsp: 3.427 ± 0.077
3.49ValGlu: 3.49 ± 0.07
2.559ValPhe: 2.559 ± 0.055
4.736ValGly: 4.736 ± 0.091
1.673ValHis: 1.673 ± 0.046
3.105ValIle: 3.105 ± 0.068
2.031ValLys: 2.031 ± 0.066
7.879ValLeu: 7.879 ± 0.117
1.788ValMet: 1.788 ± 0.053
1.814ValAsn: 1.814 ± 0.054
3.455ValPro: 3.455 ± 0.072
4.002ValGln: 4.002 ± 0.085
4.817ValArg: 4.817 ± 0.076
3.249ValSer: 3.249 ± 0.068
3.318ValThr: 3.318 ± 0.085
5.168ValVal: 5.168 ± 0.098
0.973ValTrp: 0.973 ± 0.035
1.457ValTyr: 1.457 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
1.611TrpAla: 1.611 ± 0.051
0.16TrpCys: 0.16 ± 0.014
0.653TrpAsp: 0.653 ± 0.03
0.585TrpGlu: 0.585 ± 0.024
0.551TrpPhe: 0.551 ± 0.027
1.059TrpGly: 1.059 ± 0.042
0.479TrpHis: 0.479 ± 0.026
0.632TrpIle: 0.632 ± 0.029
0.385TrpLys: 0.385 ± 0.024
2.59TrpLeu: 2.59 ± 0.078
0.425TrpMet: 0.425 ± 0.026
0.391TrpAsn: 0.391 ± 0.025
0.896TrpPro: 0.896 ± 0.036
1.307TrpGln: 1.307 ± 0.042
1.518TrpArg: 1.518 ± 0.044
0.754TrpSer: 0.754 ± 0.03
0.536TrpThr: 0.536 ± 0.026
1.011TrpVal: 1.011 ± 0.034
0.316TrpTrp: 0.316 ± 0.019
0.301TrpTyr: 0.301 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.832TyrAla: 2.832 ± 0.068
0.229TyrCys: 0.229 ± 0.018
1.203TyrAsp: 1.203 ± 0.037
1.283TyrGlu: 1.283 ± 0.04
0.891TyrPhe: 0.891 ± 0.035
2.027TyrGly: 2.027 ± 0.054
0.488TyrHis: 0.488 ± 0.025
0.893TyrIle: 0.893 ± 0.037
0.671TyrLys: 0.671 ± 0.034
2.455TyrLeu: 2.455 ± 0.053
0.403TyrMet: 0.403 ± 0.024
0.581TyrAsn: 0.581 ± 0.027
1.151TyrPro: 1.151 ± 0.046
1.019TyrGln: 1.019 ± 0.031
1.667TyrArg: 1.667 ± 0.051
1.195TyrSer: 1.195 ± 0.042
1.202TyrThr: 1.202 ± 0.041
1.572TyrVal: 1.572 ± 0.044
0.377TyrTrp: 0.377 ± 0.022
0.61TyrTyr: 0.61 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2324 proteins (793159 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski