Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) (Yeast) (Kluyveromyces thermotolerans)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Lachancea; Lachancea thermotolerans

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5093 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5DFQ3|C5DFQ3_LACTC KLTH0D17006p OS=Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) OX=559295 GN=KLTH0D17006g PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.4FSVLFSVFCASVASGMCIVRR22 pKa = 11.84FDD24 pKa = 3.64QDD26 pKa = 3.24FPIPVDD32 pKa = 3.35TTQPVGDD39 pKa = 4.61FMCNHH44 pKa = 6.8AEE46 pKa = 3.81LWLHH50 pKa = 6.05KK51 pKa = 10.26FSNGTVALEE60 pKa = 4.57SIDD63 pKa = 4.34DD64 pKa = 3.81NSIVQIIPDD73 pKa = 4.05DD74 pKa = 3.88SVFAQTAEE82 pKa = 3.99DD83 pKa = 3.59SGYY86 pKa = 10.73LQSTDD91 pKa = 4.05DD92 pKa = 6.36DD93 pKa = 4.05EE94 pKa = 6.37LEE96 pKa = 4.04WAPAA100 pKa = 3.51

Molecular weight: 11.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5DME9|C5DME9_LACTC KLTH0G08294p OS=Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) OX=559295 GN=KLTH0G08294g PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84AEE4 pKa = 4.43CRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 10.19DD9 pKa = 3.59VTSVEE14 pKa = 4.44PSTKK18 pKa = 9.83QAVARR23 pKa = 11.84APDD26 pKa = 3.63PRR28 pKa = 11.84RR29 pKa = 11.84IWACGSGAGRR39 pKa = 11.84TAGRR43 pKa = 11.84PSEE46 pKa = 4.27CMRR49 pKa = 11.84ASDD52 pKa = 3.92GAPVSRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84ARR63 pKa = 11.84GARR66 pKa = 11.84RR67 pKa = 11.84SSWMVTDD74 pKa = 2.87VHH76 pKa = 7.46VCITCAQEE84 pKa = 5.2ALDD87 pKa = 4.22EE88 pKa = 4.58SPSAFLLSNRR98 pKa = 11.84RR99 pKa = 11.84YY100 pKa = 10.14NISTARR106 pKa = 11.84ASEE109 pKa = 4.45GPLRR113 pKa = 11.84STLARR118 pKa = 11.84ARR120 pKa = 11.84CFPKK124 pKa = 10.53ANRR127 pKa = 11.84GAVDD131 pKa = 4.07FVYY134 pKa = 10.98GDD136 pKa = 3.64SDD138 pKa = 4.07YY139 pKa = 11.58KK140 pKa = 11.18GAVTNVFVYY149 pKa = 8.79FRR151 pKa = 11.84PFAPGTHH158 pKa = 5.02VFKK161 pKa = 10.72IASQLVFQKK170 pKa = 10.12MIYY173 pKa = 9.16KK174 pKa = 9.85ICGFLRR180 pKa = 11.84KK181 pKa = 9.63LWAA184 pKa = 4.52

Molecular weight: 20.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5093

0

5093

2500247

48

4928

490.9

55.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.039 ± 0.028

1.34 ± 0.014

5.591 ± 0.024

6.762 ± 0.033

4.382 ± 0.022

5.676 ± 0.028

2.193 ± 0.015

5.298 ± 0.026

6.489 ± 0.032

9.79 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.016 ± 0.011

4.62 ± 0.023

4.658 ± 0.027

4.234 ± 0.026

5.107 ± 0.027

9.004 ± 0.044

5.423 ± 0.047

6.214 ± 0.025

1.091 ± 0.01

3.075 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski