Helicobacter cinaedi CCUG 18818 = ATCC BAA-847
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2315 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7GTR3|I7GTR3_9HELI Flagellar biosynthesis protein FlhA OS=Helicobacter cinaedi CCUG 18818 = ATCC BAA-847 OX=537971 GN=flhA PE=3 SV=1
MM1 pKa = 8.18 RR2 pKa = 11.84 EE3 pKa = 3.8 PLDD6 pKa = 3.81 FKK8 pKa = 11.12 NCEE11 pKa = 4.04 LFCIGEE17 pKa = 4.22 VFFGDD22 pKa = 4.32 VEE24 pKa = 4.33 LDD26 pKa = 3.75 EE27 pKa = 5.09 PNKK30 pKa = 9.4 KK31 pKa = 10.56 ALMILDD37 pKa = 3.85 FMEE40 pKa = 5.49 FSAHH44 pKa = 5.89 KK45 pKa = 8.99 VQGRR49 pKa = 11.84 VAEE52 pKa = 4.55 EE53 pKa = 3.92 VASGYY58 pKa = 9.02 MLFDD62 pKa = 3.79 MQRR65 pKa = 11.84 KK66 pKa = 9.26 CYY68 pKa = 9.87 YY69 pKa = 10.67 DD70 pKa = 4.11 DD71 pKa = 3.72 VLGLEE76 pKa = 4.46 EE77 pKa = 5.25 PYY79 pKa = 10.44 VAQSIEE85 pKa = 4.21 ALLEE89 pKa = 4.27 MIPEE93 pKa = 4.89 AYY95 pKa = 10.33 FEE97 pKa = 5.13 DD98 pKa = 4.44 YY99 pKa = 11.06 GIEE102 pKa = 3.99 LL103 pKa = 3.98
Molecular weight: 11.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.858
IPC2_protein 4.113
IPC_protein 4.024
Toseland 3.859
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.846
Rodwell 3.859
Grimsley 3.77
Solomon 3.961
Lehninger 3.923
Nozaki 4.088
DTASelect 4.215
Thurlkill 3.872
EMBOSS 3.859
Sillero 4.126
Patrickios 1.914
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.038
Protein with the highest isoelectric point:
>tr|I7HCW4|I7HCW4_9HELI DUF2147 domain-containing protein OS=Helicobacter cinaedi CCUG 18818 = ATCC BAA-847 OX=537971 GN=HCBAA847_0944 PE=4 SV=1
MM1 pKa = 7.47 LSCVFDD7 pKa = 4.08 KK8 pKa = 10.93 EE9 pKa = 4.37 LTPSYY14 pKa = 9.98 SWSMSKK20 pKa = 10.42 NGYY23 pKa = 9.99 FIFGGAYY30 pKa = 8.88 PHH32 pKa = 7.01 ANCQEE37 pKa = 3.96 RR38 pKa = 11.84 FATQIEE44 pKa = 4.33 RR45 pKa = 11.84 LRR47 pKa = 11.84 TLGFIFGKK55 pKa = 9.08 PLKK58 pKa = 10.58 KK59 pKa = 9.73 EE60 pKa = 3.83 ACLVLQPTRR69 pKa = 11.84 LKK71 pKa = 11.18 DD72 pKa = 3.6 FVLGHH77 pKa = 6.17 SGVFLIGEE85 pKa = 3.91 AAGFINASTLEE96 pKa = 4.68 GISGAMNSSRR106 pKa = 11.84 ILAHH110 pKa = 6.86 ILNACDD116 pKa = 3.54 MNVSRR121 pKa = 11.84 TSTLHH126 pKa = 5.34 RR127 pKa = 11.84 AYY129 pKa = 10.26 RR130 pKa = 11.84 AKK132 pKa = 10.94 ARR134 pKa = 11.84 MLIVKK139 pKa = 7.82 TLFRR143 pKa = 11.84 HH144 pKa = 4.68 YY145 pKa = 10.76 VRR147 pKa = 11.84 YY148 pKa = 9.08 PFMFVPCIRR157 pKa = 11.84 RR158 pKa = 11.84 LILRR162 pKa = 11.84 FGLLRR167 pKa = 11.84 LRR169 pKa = 11.84 KK170 pKa = 9.55 GLEE173 pKa = 3.53 NGKK176 pKa = 10.15 III178 pKa = 4.87
Molecular weight: 20.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.604
IPC_protein 10.043
Toseland 10.365
ProMoST 10.043
Dawson 10.526
Bjellqvist 10.218
Wikipedia 10.701
Rodwell 10.862
Grimsley 10.584
Solomon 10.57
Lehninger 10.54
Nozaki 10.394
DTASelect 10.204
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.452
Patrickios 10.555
IPC_peptide 10.584
IPC2_peptide 9.311
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2315
0
2315
675867
14
2784
292.0
32.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.848 ± 0.053
1.3 ± 0.023
5.146 ± 0.041
6.53 ± 0.055
5.074 ± 0.042
5.976 ± 0.059
2.127 ± 0.026
8.002 ± 0.041
7.779 ± 0.055
10.34 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.343 ± 0.025
5.421 ± 0.052
3.192 ± 0.029
4.071 ± 0.033
3.651 ± 0.031
7.436 ± 0.047
4.69 ± 0.041
5.454 ± 0.051
0.774 ± 0.015
3.845 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here