Streptomyces auratus AGR0001

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces auratus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6720 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J1RIK5|J1RIK5_9ACTN Uncharacterized protein OS=Streptomyces auratus AGR0001 OX=1160718 GN=SU9_25034 PE=3 SV=1
MM1 pKa = 7.44LTTNYY6 pKa = 9.89VPGTPNWLDD15 pKa = 3.55LGAPDD20 pKa = 3.8IDD22 pKa = 3.88AAVSFYY28 pKa = 11.18SAVFGWTFQSAGPDD42 pKa = 2.94AGGYY46 pKa = 10.85GFFQLGGKK54 pKa = 6.93TVGAVGPLMEE64 pKa = 5.56EE65 pKa = 4.64GASSAWTVYY74 pKa = 10.08FQTPDD79 pKa = 2.98ADD81 pKa = 3.56ATAKK85 pKa = 10.53AVEE88 pKa = 4.17QAGGAVRR95 pKa = 11.84VAPMDD100 pKa = 3.46VFTSGRR106 pKa = 11.84LAAFTDD112 pKa = 3.62PTGADD117 pKa = 3.34FAVWQPGDD125 pKa = 3.64VQGLNTVMEE134 pKa = 5.08PNTLCWTEE142 pKa = 5.19LYY144 pKa = 7.72TTDD147 pKa = 3.68AAKK150 pKa = 10.79AKK152 pKa = 10.22DD153 pKa = 3.94FYY155 pKa = 11.6GSVFSWAYY163 pKa = 10.21QDD165 pKa = 3.77MPMGSDD171 pKa = 2.98AVYY174 pKa = 10.9SLVSAPGGGKK184 pKa = 9.89AADD187 pKa = 3.9TAHH190 pKa = 6.5GGIMQLQQEE199 pKa = 4.35NLQAGSTSEE208 pKa = 3.58WHH210 pKa = 6.99PYY212 pKa = 9.15FAVTDD217 pKa = 4.04CDD219 pKa = 3.52ATFAAATEE227 pKa = 4.27HH228 pKa = 6.79GATAMISPTDD238 pKa = 3.49APGVGRR244 pKa = 11.84LAMLMDD250 pKa = 4.62PAGAPFALIKK260 pKa = 10.92GEE262 pKa = 4.06PTTT265 pKa = 4.39

Molecular weight: 27.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J2K0X6|J2K0X6_9ACTN Precorrin-4 C(11)-methyltransferase OS=Streptomyces auratus AGR0001 OX=1160718 GN=SU9_14937 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6720

0

6720

2207359

22

6213

328.5

35.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.008 ± 0.047

0.833 ± 0.008

5.867 ± 0.026

5.523 ± 0.031

2.718 ± 0.015

9.613 ± 0.032

2.434 ± 0.016

3.109 ± 0.02

2.132 ± 0.026

10.489 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.732 ± 0.011

1.667 ± 0.015

6.296 ± 0.028

2.905 ± 0.018

8.089 ± 0.035

4.955 ± 0.025

6.058 ± 0.033

8.08 ± 0.026

1.488 ± 0.012

2.003 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski