Amino acid dipepetide frequency for Streptomyces auratus AGR0001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.078AlaAla: 23.078 ± 0.16
1.139AlaCys: 1.139 ± 0.025
8.411AlaAsp: 8.411 ± 0.075
8.727AlaGlu: 8.727 ± 0.095
3.591AlaPhe: 3.591 ± 0.043
14.128AlaGly: 14.128 ± 0.092
3.202AlaHis: 3.202 ± 0.039
3.287AlaIle: 3.287 ± 0.036
2.903AlaLys: 2.903 ± 0.058
15.265AlaLeu: 15.265 ± 0.102
2.531AlaMet: 2.531 ± 0.031
1.846AlaAsn: 1.846 ± 0.031
7.991AlaPro: 7.991 ± 0.076
4.043AlaGln: 4.043 ± 0.044
10.683AlaArg: 10.683 ± 0.085
5.522AlaSer: 5.522 ± 0.049
7.189AlaThr: 7.189 ± 0.061
12.11AlaVal: 12.11 ± 0.092
1.759AlaTrp: 1.759 ± 0.029
2.672AlaTyr: 2.672 ± 0.03
0.0AlaXaa: 0.0 ± 0.0
Cys
1.235CysAla: 1.235 ± 0.027
0.101CysCys: 0.101 ± 0.008
0.502CysAsp: 0.502 ± 0.015
0.405CysGlu: 0.405 ± 0.015
0.224CysPhe: 0.224 ± 0.012
1.013CysGly: 1.013 ± 0.024
0.206CysHis: 0.206 ± 0.009
0.21CysIle: 0.21 ± 0.01
0.13CysLys: 0.13 ± 0.008
0.788CysLeu: 0.788 ± 0.019
0.131CysMet: 0.131 ± 0.008
0.127CysAsn: 0.127 ± 0.008
0.485CysPro: 0.485 ± 0.013
0.184CysGln: 0.184 ± 0.009
0.638CysArg: 0.638 ± 0.018
0.443CysSer: 0.443 ± 0.016
0.561CysThr: 0.561 ± 0.018
0.624CysVal: 0.624 ± 0.018
0.137CysTrp: 0.137 ± 0.007
0.186CysTyr: 0.186 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.86AspAla: 7.86 ± 0.083
0.426AspCys: 0.426 ± 0.016
3.462AspAsp: 3.462 ± 0.045
3.75AspGlu: 3.75 ± 0.051
1.615AspPhe: 1.615 ± 0.027
6.542AspGly: 6.542 ± 0.065
1.492AspHis: 1.492 ± 0.025
1.796AspIle: 1.796 ± 0.03
1.174AspLys: 1.174 ± 0.027
6.033AspLeu: 6.033 ± 0.059
0.784AspMet: 0.784 ± 0.019
0.879AspAsn: 0.879 ± 0.018
4.53AspPro: 4.53 ± 0.056
1.507AspGln: 1.507 ± 0.03
4.978AspArg: 4.978 ± 0.05
2.417AspSer: 2.417 ± 0.039
3.126AspThr: 3.126 ± 0.04
4.175AspVal: 4.175 ± 0.044
1.04AspTrp: 1.04 ± 0.024
1.078AspTyr: 1.078 ± 0.026
0.0AspXaa: 0.0 ± 0.0
Glu
7.315GluAla: 7.315 ± 0.08
0.371GluCys: 0.371 ± 0.013
2.691GluAsp: 2.691 ± 0.039
3.398GluGlu: 3.398 ± 0.052
1.435GluPhe: 1.435 ± 0.024
4.167GluGly: 4.167 ± 0.05
1.423GluHis: 1.423 ± 0.028
2.213GluIle: 2.213 ± 0.031
1.332GluLys: 1.332 ± 0.028
6.833GluLeu: 6.833 ± 0.072
0.902GluMet: 0.902 ± 0.018
0.895GluAsn: 0.895 ± 0.023
3.256GluPro: 3.256 ± 0.046
2.229GluGln: 2.229 ± 0.037
5.407GluArg: 5.407 ± 0.059
2.514GluSer: 2.514 ± 0.037
2.706GluThr: 2.706 ± 0.036
4.445GluVal: 4.445 ± 0.049
0.736GluTrp: 0.736 ± 0.019
0.959GluTyr: 0.959 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.759PheAla: 3.759 ± 0.053
0.275PheCys: 0.275 ± 0.01
1.936PheAsp: 1.936 ± 0.032
1.331PheGlu: 1.331 ± 0.024
0.906PhePhe: 0.906 ± 0.021
3.004PheGly: 3.004 ± 0.04
0.669PheHis: 0.669 ± 0.019
0.706PheIle: 0.706 ± 0.018
0.48PheLys: 0.48 ± 0.018
2.684PheLeu: 2.684 ± 0.045
0.37PheMet: 0.37 ± 0.013
0.509PheAsn: 0.509 ± 0.017
1.413PhePro: 1.413 ± 0.026
0.693PheGln: 0.693 ± 0.019
1.878PheArg: 1.878 ± 0.03
1.478PheSer: 1.478 ± 0.027
2.014PheThr: 2.014 ± 0.031
2.091PheVal: 2.091 ± 0.029
0.425PheTrp: 0.425 ± 0.014
0.559PheTyr: 0.559 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.88GlyAla: 11.88 ± 0.095
0.9GlyCys: 0.9 ± 0.021
4.978GlyAsp: 4.978 ± 0.056
5.116GlyGlu: 5.116 ± 0.052
2.839GlyPhe: 2.839 ± 0.04
9.161GlyGly: 9.161 ± 0.111
2.478GlyHis: 2.478 ± 0.038
3.516GlyIle: 3.516 ± 0.042
2.514GlyLys: 2.514 ± 0.04
9.515GlyLeu: 9.515 ± 0.075
2.075GlyMet: 2.075 ± 0.03
1.706GlyAsn: 1.706 ± 0.033
5.735GlyPro: 5.735 ± 0.065
2.817GlyGln: 2.817 ± 0.042
7.99GlyArg: 7.99 ± 0.073
5.408GlySer: 5.408 ± 0.061
6.371GlyThr: 6.371 ± 0.08
7.254GlyVal: 7.254 ± 0.067
1.659GlyTrp: 1.659 ± 0.029
2.215GlyTyr: 2.215 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.784HisAla: 2.784 ± 0.039
0.251HisCys: 0.251 ± 0.012
1.406HisAsp: 1.406 ± 0.026
1.2HisGlu: 1.2 ± 0.026
0.694HisPhe: 0.694 ± 0.019
2.645HisGly: 2.645 ± 0.03
0.815HisHis: 0.815 ± 0.023
0.69HisIle: 0.69 ± 0.018
0.395HisLys: 0.395 ± 0.012
2.652HisLeu: 2.652 ± 0.039
0.337HisMet: 0.337 ± 0.012
0.371HisAsn: 0.371 ± 0.013
2.013HisPro: 2.013 ± 0.033
0.707HisGln: 0.707 ± 0.019
2.276HisArg: 2.276 ± 0.033
1.113HisSer: 1.113 ± 0.022
1.521HisThr: 1.521 ± 0.033
1.598HisVal: 1.598 ± 0.031
0.375HisTrp: 0.375 ± 0.014
0.502HisTyr: 0.502 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.78IleAla: 4.78 ± 0.06
0.311IleCys: 0.311 ± 0.011
2.16IleAsp: 2.16 ± 0.036
1.998IleGlu: 1.998 ± 0.033
0.726IlePhe: 0.726 ± 0.019
3.586IleGly: 3.586 ± 0.042
0.611IleHis: 0.611 ± 0.018
0.851IleIle: 0.851 ± 0.022
0.762IleLys: 0.762 ± 0.021
2.284IleLeu: 2.284 ± 0.036
0.443IleMet: 0.443 ± 0.017
0.718IleAsn: 0.718 ± 0.019
1.748IlePro: 1.748 ± 0.031
0.718IleGln: 0.718 ± 0.019
2.18IleArg: 2.18 ± 0.032
1.669IleSer: 1.669 ± 0.03
2.09IleThr: 2.09 ± 0.036
2.598IleVal: 2.598 ± 0.034
0.356IleTrp: 0.356 ± 0.013
0.506IleTyr: 0.506 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.865LysAla: 2.865 ± 0.05
0.114LysCys: 0.114 ± 0.008
1.355LysAsp: 1.355 ± 0.031
1.149LysGlu: 1.149 ± 0.028
0.473LysPhe: 0.473 ± 0.014
1.829LysGly: 1.829 ± 0.041
0.456LysHis: 0.456 ± 0.014
0.876LysIle: 0.876 ± 0.024
0.939LysLys: 0.939 ± 0.037
1.951LysLeu: 1.951 ± 0.039
0.429LysMet: 0.429 ± 0.014
0.526LysAsn: 0.526 ± 0.016
1.314LysPro: 1.314 ± 0.028
0.693LysGln: 0.693 ± 0.019
1.433LysArg: 1.433 ± 0.029
1.151LysSer: 1.151 ± 0.027
1.265LysThr: 1.265 ± 0.034
1.812LysVal: 1.812 ± 0.034
0.257LysTrp: 0.257 ± 0.011
0.435LysTyr: 0.435 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
15.709LeuAla: 15.709 ± 0.101
0.918LeuCys: 0.918 ± 0.022
6.748LeuAsp: 6.748 ± 0.062
4.53LeuGlu: 4.53 ± 0.048
2.624LeuPhe: 2.624 ± 0.04
9.307LeuGly: 9.307 ± 0.074
2.545LeuHis: 2.545 ± 0.038
3.312LeuIle: 3.312 ± 0.046
1.928LeuLys: 1.928 ± 0.036
11.658LeuLeu: 11.658 ± 0.104
1.674LeuMet: 1.674 ± 0.031
1.625LeuAsn: 1.625 ± 0.029
6.921LeuPro: 6.921 ± 0.068
2.402LeuGln: 2.402 ± 0.034
8.87LeuArg: 8.87 ± 0.08
5.43LeuSer: 5.43 ± 0.054
7.019LeuThr: 7.019 ± 0.064
8.566LeuVal: 8.566 ± 0.073
1.322LeuTrp: 1.322 ± 0.026
1.786LeuTyr: 1.786 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.339MetAla: 2.339 ± 0.035
0.142MetCys: 0.142 ± 0.008
0.914MetAsp: 0.914 ± 0.019
0.758MetGlu: 0.758 ± 0.02
0.436MetPhe: 0.436 ± 0.013
1.341MetGly: 1.341 ± 0.028
0.371MetHis: 0.371 ± 0.012
0.659MetIle: 0.659 ± 0.019
0.417MetLys: 0.417 ± 0.013
1.759MetLeu: 1.759 ± 0.033
0.294MetMet: 0.294 ± 0.013
0.438MetAsn: 0.438 ± 0.013
1.145MetPro: 1.145 ± 0.024
0.463MetGln: 0.463 ± 0.014
1.346MetArg: 1.346 ± 0.026
1.223MetSer: 1.223 ± 0.023
1.429MetThr: 1.429 ± 0.027
1.312MetVal: 1.312 ± 0.025
0.23MetTrp: 0.23 ± 0.01
0.303MetTyr: 0.303 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.093AsnAla: 2.093 ± 0.03
0.162AsnCys: 0.162 ± 0.008
0.928AsnAsp: 0.928 ± 0.023
0.765AsnGlu: 0.765 ± 0.019
0.471AsnPhe: 0.471 ± 0.015
1.839AsnGly: 1.839 ± 0.031
0.371AsnHis: 0.371 ± 0.014
0.624AsnIle: 0.624 ± 0.02
0.415AsnLys: 0.415 ± 0.017
1.569AsnLeu: 1.569 ± 0.026
0.278AsnMet: 0.278 ± 0.012
0.386AsnAsn: 0.386 ± 0.013
1.212AsnPro: 1.212 ± 0.026
0.484AsnGln: 0.484 ± 0.014
1.197AsnArg: 1.197 ± 0.025
0.911AsnSer: 0.911 ± 0.021
1.038AsnThr: 1.038 ± 0.022
1.257AsnVal: 1.257 ± 0.025
0.263AsnTrp: 0.263 ± 0.012
0.406AsnTyr: 0.406 ± 0.016
0.0AsnXaa: 0.0 ± 0.0
Pro
9.564ProAla: 9.564 ± 0.095
0.374ProCys: 0.374 ± 0.013
4.692ProAsp: 4.692 ± 0.054
4.297ProGlu: 4.297 ± 0.061
1.555ProPhe: 1.555 ± 0.027
6.939ProGly: 6.939 ± 0.07
1.584ProHis: 1.584 ± 0.027
1.211ProIle: 1.211 ± 0.025
1.191ProLys: 1.191 ± 0.025
5.565ProLeu: 5.565 ± 0.045
0.977ProMet: 0.977 ± 0.022
0.837ProAsn: 0.837 ± 0.021
3.801ProPro: 3.801 ± 0.058
2.103ProGln: 2.103 ± 0.041
4.106ProArg: 4.106 ± 0.052
3.201ProSer: 3.201 ± 0.041
3.126ProThr: 3.126 ± 0.045
5.492ProVal: 5.492 ± 0.053
0.936ProTrp: 0.936 ± 0.025
1.408ProTyr: 1.408 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.938GlnAla: 3.938 ± 0.045
0.199GlnCys: 0.199 ± 0.01
1.506GlnAsp: 1.506 ± 0.029
1.606GlnGlu: 1.606 ± 0.035
0.677GlnPhe: 0.677 ± 0.018
2.397GlnGly: 2.397 ± 0.035
0.766GlnHis: 0.766 ± 0.019
1.096GlnIle: 1.096 ± 0.022
0.617GlnLys: 0.617 ± 0.019
3.428GlnLeu: 3.428 ± 0.039
0.494GlnMet: 0.494 ± 0.014
0.473GlnAsn: 0.473 ± 0.015
1.808GlnPro: 1.808 ± 0.038
1.489GlnGln: 1.489 ± 0.051
2.602GlnArg: 2.602 ± 0.036
1.249GlnSer: 1.249 ± 0.027
1.36GlnThr: 1.36 ± 0.028
2.319GlnVal: 2.319 ± 0.033
0.481GlnTrp: 0.481 ± 0.015
0.548GlnTyr: 0.548 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
10.244ArgAla: 10.244 ± 0.085
0.64ArgCys: 0.64 ± 0.016
4.164ArgAsp: 4.164 ± 0.053
4.546ArgGlu: 4.546 ± 0.045
2.298ArgPhe: 2.298 ± 0.034
5.928ArgGly: 5.928 ± 0.059
2.223ArgHis: 2.223 ± 0.035
3.29ArgIle: 3.29 ± 0.036
1.692ArgLys: 1.692 ± 0.034
8.873ArgLeu: 8.873 ± 0.075
1.767ArgMet: 1.767 ± 0.03
1.341ArgAsn: 1.341 ± 0.026
5.31ArgPro: 5.31 ± 0.055
2.489ArgGln: 2.489 ± 0.033
7.804ArgArg: 7.804 ± 0.065
4.111ArgSer: 4.111 ± 0.046
5.666ArgThr: 5.666 ± 0.06
5.339ArgVal: 5.339 ± 0.053
1.369ArgTrp: 1.369 ± 0.025
1.801ArgTyr: 1.801 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.818SerAla: 6.818 ± 0.056
0.449SerCys: 0.449 ± 0.014
2.552SerAsp: 2.552 ± 0.033
2.273SerGlu: 2.273 ± 0.037
1.556SerPhe: 1.556 ± 0.025
5.975SerGly: 5.975 ± 0.056
1.074SerHis: 1.074 ± 0.021
1.347SerIle: 1.347 ± 0.023
1.008SerLys: 1.008 ± 0.026
4.773SerLeu: 4.773 ± 0.049
1.003SerMet: 1.003 ± 0.02
0.834SerAsn: 0.834 ± 0.021
3.188SerPro: 3.188 ± 0.043
1.278SerGln: 1.278 ± 0.03
3.686SerArg: 3.686 ± 0.048
2.796SerSer: 2.796 ± 0.047
2.794SerThr: 2.794 ± 0.037
4.022SerVal: 4.022 ± 0.046
0.911SerTrp: 0.911 ± 0.022
1.216SerTyr: 1.216 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
9.22ThrAla: 9.22 ± 0.074
0.433ThrCys: 0.433 ± 0.014
3.543ThrAsp: 3.543 ± 0.043
3.151ThrGlu: 3.151 ± 0.04
1.626ThrPhe: 1.626 ± 0.033
6.789ThrGly: 6.789 ± 0.063
1.253ThrHis: 1.253 ± 0.027
1.595ThrIle: 1.595 ± 0.031
1.141ThrLys: 1.141 ± 0.027
5.82ThrLeu: 5.82 ± 0.058
0.936ThrMet: 0.936 ± 0.021
0.941ThrAsn: 0.941 ± 0.023
4.068ThrPro: 4.068 ± 0.05
1.306ThrGln: 1.306 ± 0.026
3.745ThrArg: 3.745 ± 0.042
3.041ThrSer: 3.041 ± 0.037
3.819ThrThr: 3.819 ± 0.06
6.052ThrVal: 6.052 ± 0.076
0.839ThrTrp: 0.839 ± 0.022
1.264ThrTyr: 1.264 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
10.254ValAla: 10.254 ± 0.077
0.786ValCys: 0.786 ± 0.022
4.49ValAsp: 4.49 ± 0.047
4.378ValGlu: 4.378 ± 0.051
2.308ValPhe: 2.308 ± 0.034
6.236ValGly: 6.236 ± 0.066
2.019ValHis: 2.019 ± 0.029
2.854ValIle: 2.854 ± 0.041
1.577ValLys: 1.577 ± 0.035
9.486ValLeu: 9.486 ± 0.08
1.363ValMet: 1.363 ± 0.028
1.515ValAsn: 1.515 ± 0.03
5.155ValPro: 5.155 ± 0.048
2.175ValGln: 2.175 ± 0.031
6.943ValArg: 6.943 ± 0.057
4.082ValSer: 4.082 ± 0.047
5.378ValThr: 5.378 ± 0.085
7.263ValVal: 7.263 ± 0.068
1.088ValTrp: 1.088 ± 0.021
1.445ValTyr: 1.445 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.635TrpAla: 1.635 ± 0.029
0.171TrpCys: 0.171 ± 0.009
0.79TrpAsp: 0.79 ± 0.02
0.702TrpGlu: 0.702 ± 0.017
0.504TrpPhe: 0.504 ± 0.016
1.057TrpGly: 1.057 ± 0.021
0.397TrpHis: 0.397 ± 0.015
0.528TrpIle: 0.528 ± 0.013
0.34TrpLys: 0.34 ± 0.014
1.834TrpLeu: 1.834 ± 0.029
0.293TrpMet: 0.293 ± 0.013
0.342TrpAsn: 0.342 ± 0.014
0.796TrpPro: 0.796 ± 0.022
0.67TrpGln: 0.67 ± 0.018
1.312TrpArg: 1.312 ± 0.028
0.917TrpSer: 0.917 ± 0.024
0.979TrpThr: 0.979 ± 0.024
0.942TrpVal: 0.942 ± 0.022
0.335TrpTrp: 0.335 ± 0.013
0.335TrpTyr: 0.335 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.707TyrAla: 2.707 ± 0.035
0.169TyrCys: 0.169 ± 0.01
1.436TyrAsp: 1.436 ± 0.03
1.15TyrGlu: 1.15 ± 0.025
0.619TyrPhe: 0.619 ± 0.016
2.246TyrGly: 2.246 ± 0.032
0.39TyrHis: 0.39 ± 0.015
0.431TyrIle: 0.431 ± 0.016
0.365TyrLys: 0.365 ± 0.015
2.126TyrLeu: 2.126 ± 0.038
0.237TyrMet: 0.237 ± 0.011
0.358TyrAsn: 0.358 ± 0.015
1.072TyrPro: 1.072 ± 0.024
0.603TyrGln: 0.603 ± 0.019
1.817TyrArg: 1.817 ± 0.031
0.879TyrSer: 0.879 ± 0.021
1.132TyrThr: 1.132 ± 0.025
1.524TyrVal: 1.524 ± 0.027
0.362TyrTrp: 0.362 ± 0.014
0.41TyrTyr: 0.41 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6720 proteins (2207359 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski