Geoalkalibacter subterraneus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae; Geoalkalibacter

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5FLH5|A0A0B5FLH5_9DELT Uncharacterized protein OS=Geoalkalibacter subterraneus OX=483547 GN=GSUB_17585 PE=4 SV=1
MM1 pKa = 7.19SRR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 9.88LVTANVSFFVDD16 pKa = 4.32LPYY19 pKa = 10.39PEE21 pKa = 5.71NMADD25 pKa = 4.62DD26 pKa = 4.64QDD28 pKa = 4.45LQDD31 pKa = 5.54LVCCDD36 pKa = 4.02PQGEE40 pKa = 4.44MTDD43 pKa = 3.56AEE45 pKa = 4.42AVEE48 pKa = 4.67SAVTTAIRR56 pKa = 11.84YY57 pKa = 9.6CEE59 pKa = 3.94VFVYY63 pKa = 11.05GEE65 pKa = 4.2DD66 pKa = 3.87VEE68 pKa = 4.68PAQCRR73 pKa = 11.84VDD75 pKa = 4.71DD76 pKa = 3.43IDD78 pKa = 3.55MRR80 pKa = 11.84IEE82 pKa = 4.13GFEE85 pKa = 4.05DD86 pKa = 3.17

Molecular weight: 9.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5FNF8|A0A0B5FNF8_9DELT Uncharacterized protein OS=Geoalkalibacter subterraneus OX=483547 GN=GSUB_05695 PE=4 SV=1
MM1 pKa = 7.88AEE3 pKa = 3.38IRR5 pKa = 11.84AFIAVPLSEE14 pKa = 4.99KK15 pKa = 10.56NLAAIRR21 pKa = 11.84DD22 pKa = 3.73LRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84IEE28 pKa = 3.63PRR30 pKa = 11.84YY31 pKa = 8.84PQMRR35 pKa = 11.84WVRR38 pKa = 11.84TDD40 pKa = 3.47DD41 pKa = 4.11QIHH44 pKa = 5.31LTLRR48 pKa = 11.84FFAALPEE55 pKa = 4.22EE56 pKa = 4.58DD57 pKa = 4.52LDD59 pKa = 4.96RR60 pKa = 11.84IHH62 pKa = 7.32EE63 pKa = 4.44SMLSVGDD70 pKa = 3.9LNAPFSVKK78 pKa = 9.68IRR80 pKa = 11.84GLGGFPRR87 pKa = 11.84LNRR90 pKa = 11.84PRR92 pKa = 11.84VLWLGLEE99 pKa = 4.16PSAPLVALHH108 pKa = 6.55RR109 pKa = 11.84RR110 pKa = 11.84LEE112 pKa = 4.23GALRR116 pKa = 11.84RR117 pKa = 11.84EE118 pKa = 4.25GFAPEE123 pKa = 4.06EE124 pKa = 4.28RR125 pKa = 11.84PFTPHH130 pKa = 4.32VTLARR135 pKa = 11.84ARR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84GGEE142 pKa = 4.13EE143 pKa = 3.47IKK145 pKa = 10.68PPAWGRR151 pKa = 11.84SWCGGSLDD159 pKa = 3.66VNEE162 pKa = 5.09IILYY166 pKa = 10.01HH167 pKa = 6.63SRR169 pKa = 11.84LNPQGAVHH177 pKa = 6.23TPLYY181 pKa = 10.39RR182 pKa = 11.84VGLNGTSSQKK192 pKa = 8.98EE193 pKa = 3.85HH194 pKa = 6.3HH195 pKa = 6.11TT196 pKa = 3.76

Molecular weight: 22.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2987

0

2987

924840

37

3246

309.6

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.222 ± 0.053

1.281 ± 0.029

5.814 ± 0.038

7.127 ± 0.05

4.078 ± 0.035

7.824 ± 0.042

2.264 ± 0.028

5.535 ± 0.043

4.318 ± 0.049

10.559 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.024

3.072 ± 0.031

4.605 ± 0.031

3.748 ± 0.028

7.004 ± 0.055

5.512 ± 0.042

4.831 ± 0.034

7.132 ± 0.039

1.08 ± 0.021

2.601 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski