Clostridiaceae bacterium DONG20-135

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; unclassified Clostridiaceae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2223 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N8UFK5|A0A6N8UFK5_9CLOT Uncharacterized protein OS=Clostridiaceae bacterium DONG20-135 OX=2692629 GN=GSF08_08955 PE=4 SV=1
MM1 pKa = 6.74VQVKK5 pKa = 9.33VFDD8 pKa = 4.4EE9 pKa = 4.13EE10 pKa = 5.42HH11 pKa = 6.93EE12 pKa = 5.0DD13 pKa = 4.8DD14 pKa = 4.94LTDD17 pKa = 5.43AINSFLQEE25 pKa = 3.74NQAYY29 pKa = 8.95EE30 pKa = 4.6LIDD33 pKa = 3.38ISFSTAVTAVEE44 pKa = 5.12DD45 pKa = 3.71EE46 pKa = 4.32QIYY49 pKa = 10.42CFSALLVYY57 pKa = 10.52LDD59 pKa = 4.63KK60 pKa = 11.53SLDD63 pKa = 3.45

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N8U5U6|A0A6N8U5U6_9CLOT SBP_bac_5 domain-containing protein OS=Clostridiaceae bacterium DONG20-135 OX=2692629 GN=GSF08_04885 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 10.05RR10 pKa = 11.84KK11 pKa = 8.47HH12 pKa = 5.31QKK14 pKa = 6.82THH16 pKa = 4.76GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 8.95VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.59GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2223

0

2223

679347

23

2929

305.6

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.25 ± 0.05

1.444 ± 0.023

5.867 ± 0.04

6.663 ± 0.055

4.28 ± 0.038

6.286 ± 0.048

2.358 ± 0.029

8.005 ± 0.049

6.837 ± 0.056

9.578 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.336 ± 0.028

4.356 ± 0.036

3.122 ± 0.025

3.92 ± 0.035

4.216 ± 0.036

5.829 ± 0.038

5.126 ± 0.043

6.513 ± 0.043

0.809 ± 0.017

4.205 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski