Amino acid dipepetide frequency for Clostridiaceae bacterium DONG20-135

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.471AlaAla: 6.471 ± 0.135	1.191AlaCys: 1.191 ± 0.04	4.284AlaAsp: 4.284 ± 0.093	4.126AlaGlu: 4.126 ± 0.094	3.368AlaPhe: 3.368 ± 0.073	5.014AlaGly: 5.014 ± 0.107	1.393AlaHis: 1.393 ± 0.053	5.536AlaIle: 5.536 ± 0.098	4.914AlaLys: 4.914 ± 0.097	7.36AlaLeu: 7.36 ± 0.13	2.607AlaMet: 2.607 ± 0.059	2.654AlaAsn: 2.654 ± 0.063	1.708AlaPro: 1.708 ± 0.054	2.498AlaGln: 2.498 ± 0.059	2.65AlaArg: 2.65 ± 0.073	4.297AlaSer: 4.297 ± 0.081	2.812AlaThr: 2.812 ± 0.07	5.916AlaVal: 5.916 ± 0.109	0.637AlaTrp: 0.637 ± 0.037	3.071AlaTyr: 3.071 ± 0.081	0.0AlaXaa: 0.0 ± 0.0
Cys	1.051CysAla: 1.051 ± 0.038	0.262CysCys: 0.262 ± 0.023	0.999CysAsp: 0.999 ± 0.048	0.992CysGlu: 0.992 ± 0.045	0.708CysPhe: 0.708 ± 0.032	1.367CysGly: 1.367 ± 0.056	0.331CysHis: 0.331 ± 0.021	1.151CysIle: 1.151 ± 0.047	0.701CysLys: 0.701 ± 0.038	1.182CysLeu: 1.182 ± 0.042	0.477CysMet: 0.477 ± 0.025	0.47CysAsn: 0.47 ± 0.027	0.561CysPro: 0.561 ± 0.033	0.38CysGln: 0.38 ± 0.023	0.665CysArg: 0.665 ± 0.034	0.851CysSer: 0.851 ± 0.035	0.665CysThr: 0.665 ± 0.03	0.951CysVal: 0.951 ± 0.036	0.112CysTrp: 0.112 ± 0.012	0.562CysTyr: 0.562 ± 0.031	0.0CysXaa: 0.0 ± 0.0
Asp	4.195AspAla: 4.195 ± 0.091	0.802AspCys: 0.802 ± 0.041	3.475AspAsp: 3.475 ± 0.077	4.947AspGlu: 4.947 ± 0.083	2.664AspPhe: 2.664 ± 0.067	3.676AspGly: 3.676 ± 0.092	1.597AspHis: 1.597 ± 0.047	4.738AspIle: 4.738 ± 0.09	3.549AspLys: 3.549 ± 0.066	5.073AspLeu: 5.073 ± 0.103	1.989AspMet: 1.989 ± 0.053	2.168AspAsn: 2.168 ± 0.059	1.974AspPro: 1.974 ± 0.064	2.432AspGln: 2.432 ± 0.058	2.454AspArg: 2.454 ± 0.072	2.872AspSer: 2.872 ± 0.065	2.957AspThr: 2.957 ± 0.068	3.882AspVal: 3.882 ± 0.084	0.505AspTrp: 0.505 ± 0.03	2.725AspTyr: 2.725 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	5.585GluAla: 5.585 ± 0.113	0.707GluCys: 0.707 ± 0.032	3.506GluAsp: 3.506 ± 0.077	5.283GluGlu: 5.283 ± 0.111	2.199GluPhe: 2.199 ± 0.053	3.499GluGly: 3.499 ± 0.068	1.532GluHis: 1.532 ± 0.049	5.47GluIle: 5.47 ± 0.095	5.289GluLys: 5.289 ± 0.087	6.384GluLeu: 6.384 ± 0.111	2.305GluMet: 2.305 ± 0.058	3.515GluAsn: 3.515 ± 0.084	1.722GluPro: 1.722 ± 0.051	2.912GluGln: 2.912 ± 0.077	2.928GluArg: 2.928 ± 0.074	3.078GluSer: 3.078 ± 0.07	3.377GluThr: 3.377 ± 0.071	4.172GluVal: 4.172 ± 0.09	0.584GluTrp: 0.584 ± 0.029	2.585GluTyr: 2.585 ± 0.072	0.0GluXaa: 0.0 ± 0.0
Phe	2.962PheAla: 2.962 ± 0.075	0.631PheCys: 0.631 ± 0.027	2.813PheAsp: 2.813 ± 0.073	2.616PheGlu: 2.616 ± 0.064	2.02PhePhe: 2.02 ± 0.072	2.704PheGly: 2.704 ± 0.07	1.242PheHis: 1.242 ± 0.054	3.442PheIle: 3.442 ± 0.088	2.261PheLys: 2.261 ± 0.063	4.272PheLeu: 4.272 ± 0.09	1.341PheMet: 1.341 ± 0.044	1.603PheAsn: 1.603 ± 0.05	1.292PhePro: 1.292 ± 0.052	1.862PheGln: 1.862 ± 0.051	1.478PheArg: 1.478 ± 0.046	2.72PheSer: 2.72 ± 0.066	2.611PheThr: 2.611 ± 0.061	2.754PheVal: 2.754 ± 0.061	0.353PheTrp: 0.353 ± 0.023	1.824PheTyr: 1.824 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	4.223GlyAla: 4.223 ± 0.095	1.188GlyCys: 1.188 ± 0.045	3.157GlyAsp: 3.157 ± 0.08	3.536GlyGlu: 3.536 ± 0.086	3.059GlyPhe: 3.059 ± 0.072	4.144GlyGly: 4.144 ± 0.101	1.238GlyHis: 1.238 ± 0.048	5.987GlyIle: 5.987 ± 0.112	4.677GlyLys: 4.677 ± 0.092	5.436GlyLeu: 5.436 ± 0.099	2.229GlyMet: 2.229 ± 0.061	2.831GlyAsn: 2.831 ± 0.062	1.275GlyPro: 1.275 ± 0.059	1.671GlyGln: 1.671 ± 0.046	2.547GlyArg: 2.547 ± 0.066	3.902GlySer: 3.902 ± 0.087	3.592GlyThr: 3.592 ± 0.084	4.391GlyVal: 4.391 ± 0.093	0.573GlyTrp: 0.573 ± 0.029	3.202GlyTyr: 3.202 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	1.635HisAla: 1.635 ± 0.054	0.349HisCys: 0.349 ± 0.026	1.476HisAsp: 1.476 ± 0.05	1.641HisGlu: 1.641 ± 0.053	1.027HisPhe: 1.027 ± 0.043	1.529HisGly: 1.529 ± 0.049	0.762HisHis: 0.762 ± 0.038	1.892HisIle: 1.892 ± 0.052	1.313HisLys: 1.313 ± 0.044	2.002HisLeu: 2.002 ± 0.058	0.695HisMet: 0.695 ± 0.031	0.919HisAsn: 0.919 ± 0.035	1.044HisPro: 1.044 ± 0.039	1.042HisGln: 1.042 ± 0.039	1.033HisArg: 1.033 ± 0.041	1.232HisSer: 1.232 ± 0.045	1.267HisThr: 1.267 ± 0.052	1.566HisVal: 1.566 ± 0.05	0.16HisTrp: 0.16 ± 0.014	0.991HisTyr: 0.991 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	6.246IleAla: 6.246 ± 0.115	1.316IleCys: 1.316 ± 0.05	5.061IleAsp: 5.061 ± 0.093	4.975IleGlu: 4.975 ± 0.091	3.134IlePhe: 3.134 ± 0.079	5.416IleGly: 5.416 ± 0.11	1.992IleHis: 1.992 ± 0.067	5.778IleIle: 5.778 ± 0.129	4.578IleLys: 4.578 ± 0.09	7.641IleLeu: 7.641 ± 0.125	2.457IleMet: 2.457 ± 0.057	3.216IleAsn: 3.216 ± 0.075	2.982IlePro: 2.982 ± 0.071	3.166IleGln: 3.166 ± 0.079	3.536IleArg: 3.536 ± 0.077	5.206IleSer: 5.206 ± 0.101	4.669IleThr: 4.669 ± 0.1	5.14IleVal: 5.14 ± 0.106	0.601IleTrp: 0.601 ± 0.034	2.94IleTyr: 2.94 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	5.343LysAla: 5.343 ± 0.097	0.623LysCys: 0.623 ± 0.034	4.236LysAsp: 4.236 ± 0.093	5.807LysGlu: 5.807 ± 0.105	1.766LysPhe: 1.766 ± 0.047	3.895LysGly: 3.895 ± 0.08	1.519LysHis: 1.519 ± 0.052	4.628LysIle: 4.628 ± 0.078	5.686LysLys: 5.686 ± 0.108	5.982LysLeu: 5.982 ± 0.101	2.239LysMet: 2.239 ± 0.052	3.417LysAsn: 3.417 ± 0.076	2.177LysPro: 2.177 ± 0.067	3.729LysGln: 3.729 ± 0.084	3.33LysArg: 3.33 ± 0.065	3.28LysSer: 3.28 ± 0.077	3.861LysThr: 3.861 ± 0.08	4.008LysVal: 4.008 ± 0.079	0.512LysTrp: 0.512 ± 0.027	2.335LysTyr: 2.335 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	6.606LeuAla: 6.606 ± 0.102	1.538LeuCys: 1.538 ± 0.053	5.654LeuAsp: 5.654 ± 0.095	5.895LeuGlu: 5.895 ± 0.117	4.223LeuPhe: 4.223 ± 0.091	5.65LeuGly: 5.65 ± 0.105	2.501LeuHis: 2.501 ± 0.068	7.419LeuIle: 7.419 ± 0.133	7.13LeuLys: 7.13 ± 0.101	9.892LeuLeu: 9.892 ± 0.173	2.971LeuMet: 2.971 ± 0.063	4.372LeuAsn: 4.372 ± 0.074	3.518LeuPro: 3.518 ± 0.081	3.493LeuGln: 3.493 ± 0.072	3.739LeuArg: 3.739 ± 0.085	6.617LeuSer: 6.617 ± 0.116	4.956LeuThr: 4.956 ± 0.084	5.274LeuVal: 5.274 ± 0.092	0.651LeuTrp: 0.651 ± 0.034	3.679LeuTyr: 3.679 ± 0.081	0.0LeuXaa: 0.0 ± 0.0
Met	2.065MetAla: 2.065 ± 0.054	0.331MetCys: 0.331 ± 0.022	2.021MetAsp: 2.021 ± 0.05	2.379MetGlu: 2.379 ± 0.058	1.148MetPhe: 1.148 ± 0.045	1.911MetGly: 1.911 ± 0.06	0.687MetHis: 0.687 ± 0.034	2.888MetIle: 2.888 ± 0.064	3.169MetLys: 3.169 ± 0.07	3.182MetLeu: 3.182 ± 0.069	1.267MetMet: 1.267 ± 0.047	1.917MetAsn: 1.917 ± 0.053	1.132MetPro: 1.132 ± 0.038	1.291MetGln: 1.291 ± 0.044	1.428MetArg: 1.428 ± 0.049	1.93MetSer: 1.93 ± 0.057	1.45MetThr: 1.45 ± 0.054	1.955MetVal: 1.955 ± 0.061	0.155MetTrp: 0.155 ± 0.015	1.054MetTyr: 1.054 ± 0.04	0.0MetXaa: 0.0 ± 0.0
Asn	3.207AsnAla: 3.207 ± 0.078	0.53AsnCys: 0.53 ± 0.026	2.626AsnAsp: 2.626 ± 0.066	3.062AsnGlu: 3.062 ± 0.08	1.625AsnPhe: 1.625 ± 0.052	3.156AsnGly: 3.156 ± 0.083	1.234AsnHis: 1.234 ± 0.039	3.331AsnIle: 3.331 ± 0.074	2.604AsnLys: 2.604 ± 0.071	3.78AsnLeu: 3.78 ± 0.076	1.409AsnMet: 1.409 ± 0.044	1.73AsnAsn: 1.73 ± 0.066	1.794AsnPro: 1.794 ± 0.047	1.868AsnGln: 1.868 ± 0.054	2.106AsnArg: 2.106 ± 0.05	2.195AsnSer: 2.195 ± 0.055	2.285AsnThr: 2.285 ± 0.06	2.764AsnVal: 2.764 ± 0.06	0.427AsnTrp: 0.427 ± 0.026	1.828AsnTyr: 1.828 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	2.133ProAla: 2.133 ± 0.065	0.411ProCys: 0.411 ± 0.029	1.981ProAsp: 1.981 ± 0.056	1.964ProGlu: 1.964 ± 0.065	1.646ProPhe: 1.646 ± 0.057	1.812ProGly: 1.812 ± 0.053	0.665ProHis: 0.665 ± 0.035	2.753ProIle: 2.753 ± 0.073	2.011ProLys: 2.011 ± 0.048	3.087ProLeu: 3.087 ± 0.068	0.949ProMet: 0.949 ± 0.041	1.46ProAsn: 1.46 ± 0.043	0.605ProPro: 0.605 ± 0.031	1.301ProGln: 1.301 ± 0.047	1.001ProArg: 1.001 ± 0.046	1.79ProSer: 1.79 ± 0.055	1.524ProThr: 1.524 ± 0.05	2.268ProVal: 2.268 ± 0.055	0.277ProTrp: 0.277 ± 0.023	1.581ProTyr: 1.581 ± 0.05	0.0ProXaa: 0.0 ± 0.0
Gln	2.918GlnAla: 2.918 ± 0.069	0.406GlnCys: 0.406 ± 0.023	2.162GlnAsp: 2.162 ± 0.069	2.931GlnGlu: 2.931 ± 0.073	1.541GlnPhe: 1.541 ± 0.049	2.118GlnGly: 2.118 ± 0.061	0.811GlnHis: 0.811 ± 0.034	3.115GlnIle: 3.115 ± 0.069	3.047GlnLys: 3.047 ± 0.071	4.001GlnLeu: 4.001 ± 0.083	1.298GlnMet: 1.298 ± 0.048	1.868GlnAsn: 1.868 ± 0.06	1.148GlnPro: 1.148 ± 0.049	1.708GlnGln: 1.708 ± 0.057	1.847GlnArg: 1.847 ± 0.06	2.065GlnSer: 2.065 ± 0.056	1.89GlnThr: 1.89 ± 0.052	2.349GlnVal: 2.349 ± 0.054	0.268GlnTrp: 0.268 ± 0.021	1.706GlnTyr: 1.706 ± 0.053	0.0GlnXaa: 0.0 ± 0.0
Arg	2.299ArgAla: 2.299 ± 0.059	0.67ArgCys: 0.67 ± 0.036	2.282ArgAsp: 2.282 ± 0.065	2.934ArgGlu: 2.934 ± 0.066	1.962ArgPhe: 1.962 ± 0.055	2.184ArgGly: 2.184 ± 0.048	0.954ArgHis: 0.954 ± 0.035	3.729ArgIle: 3.729 ± 0.079	3.163ArgLys: 3.163 ± 0.074	3.867ArgLeu: 3.867 ± 0.09	1.577ArgMet: 1.577 ± 0.045	2.042ArgAsn: 2.042 ± 0.055	1.213ArgPro: 1.213 ± 0.052	1.681ArgGln: 1.681 ± 0.05	2.064ArgArg: 2.064 ± 0.058	2.505ArgSer: 2.505 ± 0.064	2.093ArgThr: 2.093 ± 0.056	2.508ArgVal: 2.508 ± 0.063	0.321ArgTrp: 0.321 ± 0.025	2.117ArgTyr: 2.117 ± 0.064	0.0ArgXaa: 0.0 ± 0.0
Ser	3.986SerAla: 3.986 ± 0.087	0.896SerCys: 0.896 ± 0.041	3.54SerAsp: 3.54 ± 0.078	3.468SerGlu: 3.468 ± 0.074	3.038SerPhe: 3.038 ± 0.068	4.534SerGly: 4.534 ± 0.092	1.217SerHis: 1.217 ± 0.043	4.635SerIle: 4.635 ± 0.106	3.724SerLys: 3.724 ± 0.072	5.658SerLeu: 5.658 ± 0.106	1.995SerMet: 1.995 ± 0.058	2.357SerAsn: 2.357 ± 0.073	1.415SerPro: 1.415 ± 0.041	1.981SerGln: 1.981 ± 0.058	2.511SerArg: 2.511 ± 0.055	3.571SerSer: 3.571 ± 0.086	2.761SerThr: 2.761 ± 0.068	3.932SerVal: 3.932 ± 0.08	0.545SerTrp: 0.545 ± 0.028	2.522SerTyr: 2.522 ± 0.071	0.0SerXaa: 0.0 ± 0.0
Thr	4.158ThrAla: 4.158 ± 0.089	0.721ThrCys: 0.721 ± 0.036	2.669ThrAsp: 2.669 ± 0.065	2.492ThrGlu: 2.492 ± 0.059	2.446ThrPhe: 2.446 ± 0.067	3.468ThrGly: 3.468 ± 0.081	1.072ThrHis: 1.072 ± 0.036	4.41ThrIle: 4.41 ± 0.098	3.3ThrLys: 3.3 ± 0.074	5.195ThrLeu: 5.195 ± 0.091	1.702ThrMet: 1.702 ± 0.046	1.98ThrAsn: 1.98 ± 0.059	1.943ThrPro: 1.943 ± 0.06	1.535ThrGln: 1.535 ± 0.049	1.841ThrArg: 1.841 ± 0.048	3.187ThrSer: 3.187 ± 0.077	2.479ThrThr: 2.479 ± 0.068	3.889ThrVal: 3.889 ± 0.071	0.464ThrTrp: 0.464 ± 0.026	2.305ThrTyr: 2.305 ± 0.067	0.0ThrXaa: 0.0 ± 0.0
Val	4.073ValAla: 4.073 ± 0.099	1.176ValCys: 1.176 ± 0.042	3.611ValAsp: 3.611 ± 0.08	3.899ValGlu: 3.899 ± 0.088	2.885ValPhe: 2.885 ± 0.073	3.596ValGly: 3.596 ± 0.083	1.373ValHis: 1.373 ± 0.051	5.607ValIle: 5.607 ± 0.104	4.64ValLys: 4.64 ± 0.098	6.726ValLeu: 6.726 ± 0.112	2.277ValMet: 2.277 ± 0.063	3.069ValAsn: 3.069 ± 0.067	2.003ValPro: 2.003 ± 0.064	2.083ValGln: 2.083 ± 0.053	2.591ValArg: 2.591 ± 0.067	4.473ValSer: 4.473 ± 0.09	3.497ValThr: 3.497 ± 0.074	4.319ValVal: 4.319 ± 0.093	0.52ValTrp: 0.52 ± 0.027	2.716ValTyr: 2.716 ± 0.063	0.0ValXaa: 0.0 ± 0.0
Trp	0.405TrpAla: 0.405 ± 0.025	0.131TrpCys: 0.131 ± 0.02	0.393TrpAsp: 0.393 ± 0.022	0.493TrpGlu: 0.493 ± 0.024	0.39TrpPhe: 0.39 ± 0.023	0.486TrpGly: 0.486 ± 0.029	0.188TrpHis: 0.188 ± 0.018	0.749TrpIle: 0.749 ± 0.037	0.696TrpLys: 0.696 ± 0.029	0.883TrpLeu: 0.883 ± 0.041	0.356TrpMet: 0.356 ± 0.026	0.461TrpAsn: 0.461 ± 0.027	0.196TrpPro: 0.196 ± 0.016	0.306TrpGln: 0.306 ± 0.022	0.274TrpArg: 0.274 ± 0.022	0.397TrpSer: 0.397 ± 0.024	0.364TrpThr: 0.364 ± 0.028	0.484TrpVal: 0.484 ± 0.026	0.081TrpTrp: 0.081 ± 0.011	0.355TrpTyr: 0.355 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.941TyrAla: 2.941 ± 0.071	0.559TyrCys: 0.559 ± 0.032	2.726TyrAsp: 2.726 ± 0.067	3.191TyrGlu: 3.191 ± 0.064	1.95TyrPhe: 1.95 ± 0.064	2.698TyrGly: 2.698 ± 0.068	1.267TyrHis: 1.267 ± 0.047	2.792TyrIle: 2.792 ± 0.066	1.921TyrLys: 1.921 ± 0.056	4.175TyrLeu: 4.175 ± 0.085	1.22TyrMet: 1.22 ± 0.038	1.515TyrAsn: 1.515 ± 0.054	1.516TyrPro: 1.516 ± 0.053	2.26TyrGln: 2.26 ± 0.065	2.142TyrArg: 2.142 ± 0.059	2.118TyrSer: 2.118 ± 0.056	2.146TyrThr: 2.146 ± 0.058	2.611TyrVal: 2.611 ± 0.067	0.344TyrTrp: 0.344 ± 0.022	1.956TyrTyr: 1.956 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2223 proteins (679347 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---