Blattella germanica (German cockroach) (Blatta germanica)
Average proteome isoelectric point is 7.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 29020 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P8Z619|A0A2P8Z619_BLAGE Uncharacterized protein OS=Blattella germanica OX=6973 GN=C0J52_06301 PE=4 SV=1
MM1 pKa = 7.52 FYY3 pKa = 11.02 KK4 pKa = 10.69 FLTLILAVVLLFAVITSAFKK24 pKa = 10.85 HH25 pKa = 6.48 CDD27 pKa = 3.06 LTRR30 pKa = 11.84 NNCLVDD36 pKa = 3.53 SGAYY40 pKa = 9.99 DD41 pKa = 3.42 SLQVPEE47 pKa = 3.98 EE48 pKa = 4.3 HH49 pKa = 6.9 YY50 pKa = 11.17 FGDD53 pKa = 4.22 FHH55 pKa = 7.29 PLLQIDD61 pKa = 4.95 DD62 pKa = 4.61 SDD64 pKa = 6.16 DD65 pKa = 3.59 DD66 pKa = 4.31 TLTNTDD72 pKa = 4.73 DD73 pKa = 3.57 EE74 pKa = 4.53 DD75 pKa = 5.9 LYY77 pKa = 11.23 MEE79 pKa = 4.91 VDD81 pKa = 3.49 QIMTMFHH88 pKa = 6.96 SDD90 pKa = 3.73 GEE92 pKa = 4.43 DD93 pKa = 3.98 GEE95 pKa = 5.51 CEE97 pKa = 4.01 DD98 pKa = 4.86 PEE100 pKa = 4.42 EE101 pKa = 4.71 EE102 pKa = 4.92 EE103 pKa = 3.93 MDD105 pKa = 3.65 NVNVKK110 pKa = 10.1 NVEE113 pKa = 3.83 EE114 pKa = 4.45 SNDD117 pKa = 3.52 DD118 pKa = 4.18 KK119 pKa = 11.74 EE120 pKa = 4.71 PGSCPRR126 pKa = 11.84 AEE128 pKa = 4.18 TDD130 pKa = 4.18 QEE132 pKa = 4.31 DD133 pKa = 4.05 DD134 pKa = 3.83 HH135 pKa = 9.04 DD136 pKa = 5.57 LICEE140 pKa = 4.71 AIKK143 pKa = 10.44 EE144 pKa = 4.4 SHH146 pKa = 5.14 QQMM149 pKa = 3.91
Molecular weight: 17.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.528
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.872
Patrickios 1.125
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A2P8YHX5|A0A2P8YHX5_BLAGE Uncharacterized protein OS=Blattella germanica OX=6973 GN=C0J52_15509 PE=4 SV=1
MM1 pKa = 7.29 RR2 pKa = 11.84 TTVILRR8 pKa = 11.84 PMRR11 pKa = 11.84 SSWSRR16 pKa = 11.84 WSVISIMFFWSIFRR30 pKa = 11.84 FRR32 pKa = 11.84 TFWLSGMRR40 pKa = 11.84 TMVILWSMRR49 pKa = 11.84 SSWSSWSVRR58 pKa = 11.84 TFRR61 pKa = 11.84 TFWSIFRR68 pKa = 11.84 FRR70 pKa = 11.84 TFWLSSMRR78 pKa = 11.84 SMMIFRR84 pKa = 11.84 SSRR87 pKa = 11.84 SSWSLWSVKK96 pKa = 7.77 SFRR99 pKa = 11.84 FILRR103 pKa = 11.84 IITTRR108 pKa = 11.84 LAWFFKK114 pKa = 9.28 STWSVWLIIGFLSNRR129 pKa = 11.84 LSIWCGFILRR139 pKa = 11.84 FHH141 pKa = 6.97
Molecular weight: 17.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.514
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.501
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.223
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.189
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28575
445
29020
8958027
51
8376
308.7
34.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.699 ± 0.018
2.166 ± 0.017
5.093 ± 0.014
6.634 ± 0.025
4.011 ± 0.015
5.613 ± 0.024
2.701 ± 0.009
5.682 ± 0.016
6.365 ± 0.024
9.213 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.009
4.946 ± 0.016
5.042 ± 0.024
4.115 ± 0.015
5.481 ± 0.017
8.314 ± 0.028
5.823 ± 0.02
6.355 ± 0.014
1.18 ± 0.006
3.137 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here