uncultured phage_MedDCM-OCT-S45-C18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Ayaqvirus; Ayaqvirus S45C18

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4PCW6|A0A6S4PCW6_9CAUD DNA-directed DNA polymerase OS=uncultured phage_MedDCM-OCT-S45-C18 OX=2741072 PE=3 SV=1
MM1 pKa = 7.69SLEE4 pKa = 4.1EE5 pKa = 3.8QAEE8 pKa = 4.37MQEE11 pKa = 4.55SAQQATEE18 pKa = 4.03QQPEE22 pKa = 4.08TAEE25 pKa = 4.22ASPDD29 pKa = 3.45RR30 pKa = 11.84PEE32 pKa = 3.84WLPEE36 pKa = 3.91KK37 pKa = 10.33FDD39 pKa = 3.89NPEE42 pKa = 4.01ALADD46 pKa = 4.02AYY48 pKa = 11.69SNLEE52 pKa = 4.07KK53 pKa = 10.64QFHH56 pKa = 5.51EE57 pKa = 4.51NKK59 pKa = 10.25AEE61 pKa = 4.09PSEE64 pKa = 4.36TEE66 pKa = 4.21DD67 pKa = 3.44NATSEE72 pKa = 4.65PEE74 pKa = 4.02VSNTAVTSASEE85 pKa = 4.52EE86 pKa = 4.07YY87 pKa = 10.84FEE89 pKa = 4.34TGEE92 pKa = 4.54LSEE95 pKa = 4.26EE96 pKa = 4.62TYY98 pKa = 11.06KK99 pKa = 11.05SLEE102 pKa = 3.92ANGIPKK108 pKa = 10.21EE109 pKa = 4.02MVDD112 pKa = 3.6MYY114 pKa = 11.34VNGYY118 pKa = 8.65EE119 pKa = 4.34AVANQQQQTLMQEE132 pKa = 4.01AGGKK136 pKa = 8.82EE137 pKa = 4.12NYY139 pKa = 10.12DD140 pKa = 4.64AMSEE144 pKa = 4.01WAATALTDD152 pKa = 4.87QEE154 pKa = 4.36QEE156 pKa = 4.23VYY158 pKa = 11.24NNTVEE163 pKa = 4.35SGDD166 pKa = 3.6VNAATMAIRR175 pKa = 11.84GLYY178 pKa = 10.45ARR180 pKa = 11.84FQSDD184 pKa = 2.57GGTPVSLVQGDD195 pKa = 3.87TSGTAGALPFSSSKK209 pKa = 10.86EE210 pKa = 3.76MTIAMQDD217 pKa = 3.08PRR219 pKa = 11.84YY220 pKa = 10.58SYY222 pKa = 10.9DD223 pKa = 3.04NKK225 pKa = 10.47YY226 pKa = 10.4RR227 pKa = 11.84EE228 pKa = 4.23QVSQRR233 pKa = 11.84LSVTTAFF240 pKa = 4.24

Molecular weight: 26.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PM01|A0A6S4PM01_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S45-C18 OX=2741072 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.18SLLMTTEE9 pKa = 4.26LFRR12 pKa = 11.84RR13 pKa = 11.84ANINTMYY20 pKa = 10.64RR21 pKa = 11.84ASLCIAVVAKK31 pKa = 10.37PGITNTKK38 pKa = 8.26LAAMMRR44 pKa = 11.84TSRR47 pKa = 11.84EE48 pKa = 4.17SIQVALRR55 pKa = 11.84NLIKK59 pKa = 10.67EE60 pKa = 4.27NLVHH64 pKa = 5.05VTKK67 pKa = 10.61IIDD70 pKa = 3.47KK71 pKa = 7.39EE72 pKa = 4.46TNRR75 pKa = 11.84PKK77 pKa = 9.1EE78 pKa = 4.32TKK80 pKa = 10.2VFPTPYY86 pKa = 9.87LKK88 pKa = 10.71DD89 pKa = 3.59VIAGITNLTTTTQKK103 pKa = 11.32

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

10912

39

1004

279.8

31.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.211 ± 0.473

0.889 ± 0.172

6.736 ± 0.298

6.461 ± 0.636

3.821 ± 0.257

6.919 ± 0.383

1.76 ± 0.277

5.114 ± 0.302

5.975 ± 0.539

8.752 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.282

5.086 ± 0.336

4.014 ± 0.234

3.95 ± 0.306

4.985 ± 0.454

7.084 ± 0.601

7.24 ± 0.555

6.25 ± 0.278

1.338 ± 0.216

3.18 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski