Amino acid dipepetide frequency for uncultured phage_MedDCM-OCT-S45-C18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.424AlaAla: 7.424 ± 1.268
0.733AlaCys: 0.733 ± 0.232
5.774AlaAsp: 5.774 ± 0.629
5.132AlaGlu: 5.132 ± 0.917
2.291AlaPhe: 2.291 ± 0.458
5.866AlaGly: 5.866 ± 0.756
1.375AlaHis: 1.375 ± 0.434
4.124AlaIle: 4.124 ± 0.525
4.766AlaLys: 4.766 ± 0.736
7.974AlaLeu: 7.974 ± 0.916
1.925AlaMet: 1.925 ± 0.332
4.674AlaAsn: 4.674 ± 0.602
2.475AlaPro: 2.475 ± 0.389
3.758AlaGln: 3.758 ± 0.892
3.758AlaArg: 3.758 ± 0.577
5.866AlaSer: 5.866 ± 0.639
6.416AlaThr: 6.416 ± 1.123
4.124AlaVal: 4.124 ± 0.688
1.466AlaTrp: 1.466 ± 0.53
2.2AlaTyr: 2.2 ± 0.435
0.0AlaXaa: 0.0 ± 0.0
Cys
0.55CysAla: 0.55 ± 0.187
0.0CysCys: 0.0 ± 0.0
0.733CysAsp: 0.733 ± 0.25
1.1CysGlu: 1.1 ± 0.368
0.55CysPhe: 0.55 ± 0.371
0.367CysGly: 0.367 ± 0.194
0.275CysHis: 0.275 ± 0.19
0.367CysIle: 0.367 ± 0.205
0.55CysLys: 0.55 ± 0.279
0.642CysLeu: 0.642 ± 0.251
0.183CysMet: 0.183 ± 0.145
0.275CysAsn: 0.275 ± 0.158
0.367CysPro: 0.367 ± 0.22
0.092CysGln: 0.092 ± 0.093
0.642CysArg: 0.642 ± 0.269
0.458CysSer: 0.458 ± 0.178
0.642CysThr: 0.642 ± 0.317
0.642CysVal: 0.642 ± 0.203
0.183CysTrp: 0.183 ± 0.134
0.275CysTyr: 0.275 ± 0.166
0.0CysXaa: 0.0 ± 0.0
Asp
5.407AspAla: 5.407 ± 0.597
0.458AspCys: 0.458 ± 0.23
4.308AspAsp: 4.308 ± 0.696
4.857AspGlu: 4.857 ± 0.953
3.574AspPhe: 3.574 ± 0.611
5.957AspGly: 5.957 ± 0.831
1.191AspHis: 1.191 ± 0.342
3.116AspIle: 3.116 ± 0.523
3.574AspLys: 3.574 ± 0.596
5.774AspLeu: 5.774 ± 0.669
1.008AspMet: 1.008 ± 0.329
3.574AspAsn: 3.574 ± 0.607
3.116AspPro: 3.116 ± 0.464
1.375AspGln: 1.375 ± 0.331
3.116AspArg: 3.116 ± 0.631
3.941AspSer: 3.941 ± 0.813
4.308AspThr: 4.308 ± 0.643
5.499AspVal: 5.499 ± 0.75
0.917AspTrp: 0.917 ± 0.321
2.291AspTyr: 2.291 ± 0.45
0.0AspXaa: 0.0 ± 0.0
Glu
6.599GluAla: 6.599 ± 0.911
0.55GluCys: 0.55 ± 0.269
3.666GluAsp: 3.666 ± 0.629
5.224GluGlu: 5.224 ± 0.937
2.291GluPhe: 2.291 ± 0.384
4.216GluGly: 4.216 ± 0.713
0.825GluHis: 0.825 ± 0.316
3.666GluIle: 3.666 ± 0.797
3.391GluLys: 3.391 ± 0.771
5.682GluLeu: 5.682 ± 0.925
2.291GluMet: 2.291 ± 0.566
2.2GluAsn: 2.2 ± 0.576
2.383GluPro: 2.383 ± 0.502
2.933GluGln: 2.933 ± 0.724
3.391GluArg: 3.391 ± 0.819
3.849GluSer: 3.849 ± 0.508
4.399GluThr: 4.399 ± 0.789
2.841GluVal: 2.841 ± 0.599
2.108GluTrp: 2.108 ± 0.648
2.016GluTyr: 2.016 ± 0.551
0.0GluXaa: 0.0 ± 0.0
Phe
2.475PheAla: 2.475 ± 0.504
0.458PheCys: 0.458 ± 0.285
3.391PheAsp: 3.391 ± 0.445
2.108PheGlu: 2.108 ± 0.497
1.65PhePhe: 1.65 ± 0.507
2.383PheGly: 2.383 ± 0.508
0.367PheHis: 0.367 ± 0.187
1.65PheIle: 1.65 ± 0.451
1.466PheLys: 1.466 ± 0.354
2.658PheLeu: 2.658 ± 0.581
0.917PheMet: 0.917 ± 0.314
1.925PheAsn: 1.925 ± 0.454
1.558PhePro: 1.558 ± 0.452
1.558PheGln: 1.558 ± 0.322
2.2PheArg: 2.2 ± 0.6
4.491PheSer: 4.491 ± 0.91
2.841PheThr: 2.841 ± 0.43
2.566PheVal: 2.566 ± 0.708
0.458PheTrp: 0.458 ± 0.186
1.1PheTyr: 1.1 ± 0.417
0.0PheXaa: 0.0 ± 0.0
Gly
5.957GlyAla: 5.957 ± 0.921
0.367GlyCys: 0.367 ± 0.213
5.224GlyAsp: 5.224 ± 0.951
3.391GlyGlu: 3.391 ± 0.506
2.475GlyPhe: 2.475 ± 0.492
4.949GlyGly: 4.949 ± 0.938
0.917GlyHis: 0.917 ± 0.351
3.391GlyIle: 3.391 ± 0.629
4.766GlyLys: 4.766 ± 0.965
6.782GlyLeu: 6.782 ± 0.715
1.008GlyMet: 1.008 ± 0.354
3.941GlyAsn: 3.941 ± 0.845
1.466GlyPro: 1.466 ± 0.372
2.2GlyGln: 2.2 ± 0.461
3.208GlyArg: 3.208 ± 0.779
6.416GlySer: 6.416 ± 1.163
3.849GlyThr: 3.849 ± 1.094
5.224GlyVal: 5.224 ± 0.715
1.1GlyTrp: 1.1 ± 0.392
2.566GlyTyr: 2.566 ± 0.412
0.0GlyXaa: 0.0 ± 0.0
His
1.1HisAla: 1.1 ± 0.376
0.183HisCys: 0.183 ± 0.125
1.1HisAsp: 1.1 ± 0.371
1.008HisGlu: 1.008 ± 0.272
0.825HisPhe: 0.825 ± 0.323
1.008HisGly: 1.008 ± 0.355
0.642HisHis: 0.642 ± 0.272
0.733HisIle: 0.733 ± 0.38
1.1HisLys: 1.1 ± 0.251
1.833HisLeu: 1.833 ± 0.509
0.733HisMet: 0.733 ± 0.272
0.825HisAsn: 0.825 ± 0.263
0.825HisPro: 0.825 ± 0.244
0.733HisGln: 0.733 ± 0.292
0.733HisArg: 0.733 ± 0.206
1.466HisSer: 1.466 ± 0.471
1.283HisThr: 1.283 ± 0.321
0.642HisVal: 0.642 ± 0.272
0.367HisTrp: 0.367 ± 0.186
0.458HisTyr: 0.458 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
4.949IleAla: 4.949 ± 0.83
0.275IleCys: 0.275 ± 0.173
4.583IleAsp: 4.583 ± 0.722
2.658IleGlu: 2.658 ± 0.687
1.191IlePhe: 1.191 ± 0.312
2.566IleGly: 2.566 ± 0.495
1.1IleHis: 1.1 ± 0.384
2.566IleIle: 2.566 ± 0.705
3.574IleLys: 3.574 ± 0.542
3.849IleLeu: 3.849 ± 0.644
0.917IleMet: 0.917 ± 0.284
3.483IleAsn: 3.483 ± 0.5
2.2IlePro: 2.2 ± 0.468
2.291IleGln: 2.291 ± 0.469
2.383IleArg: 2.383 ± 0.331
2.933IleSer: 2.933 ± 0.419
4.857IleThr: 4.857 ± 0.893
2.841IleVal: 2.841 ± 0.534
0.183IleTrp: 0.183 ± 0.145
1.741IleTyr: 1.741 ± 0.36
0.0IleXaa: 0.0 ± 0.0
Lys
4.766LysAla: 4.766 ± 0.683
0.367LysCys: 0.367 ± 0.161
4.674LysAsp: 4.674 ± 0.695
3.849LysGlu: 3.849 ± 1.406
1.466LysPhe: 1.466 ± 0.327
3.208LysGly: 3.208 ± 0.591
1.375LysHis: 1.375 ± 0.347
3.574LysIle: 3.574 ± 0.798
3.849LysLys: 3.849 ± 0.693
6.049LysLeu: 6.049 ± 0.938
2.016LysMet: 2.016 ± 0.53
2.291LysAsn: 2.291 ± 0.46
2.2LysPro: 2.2 ± 0.411
3.299LysGln: 3.299 ± 0.595
3.666LysArg: 3.666 ± 0.496
3.208LysSer: 3.208 ± 0.473
3.849LysThr: 3.849 ± 0.937
3.849LysVal: 3.849 ± 0.595
0.458LysTrp: 0.458 ± 0.202
1.741LysTyr: 1.741 ± 0.418
0.0LysXaa: 0.0 ± 0.0
Leu
6.232LeuAla: 6.232 ± 0.765
0.55LeuCys: 0.55 ± 0.355
5.591LeuAsp: 5.591 ± 0.593
7.24LeuGlu: 7.24 ± 0.856
3.208LeuPhe: 3.208 ± 0.563
5.682LeuGly: 5.682 ± 0.747
1.833LeuHis: 1.833 ± 0.362
4.216LeuIle: 4.216 ± 0.505
6.232LeuLys: 6.232 ± 1.004
6.324LeuLeu: 6.324 ± 1.038
2.383LeuMet: 2.383 ± 0.507
4.308LeuAsn: 4.308 ± 0.797
5.316LeuPro: 5.316 ± 1.038
2.383LeuGln: 2.383 ± 0.467
5.132LeuArg: 5.132 ± 0.572
6.141LeuSer: 6.141 ± 0.68
6.69LeuThr: 6.69 ± 1.145
4.857LeuVal: 4.857 ± 0.554
0.642LeuTrp: 0.642 ± 0.273
2.566LeuTyr: 2.566 ± 0.462
0.0LeuXaa: 0.0 ± 0.0
Met
1.558MetAla: 1.558 ± 0.305
0.367MetCys: 0.367 ± 0.176
0.825MetAsp: 0.825 ± 0.291
1.375MetGlu: 1.375 ± 0.348
1.008MetPhe: 1.008 ± 0.289
1.008MetGly: 1.008 ± 0.289
0.55MetHis: 0.55 ± 0.223
0.917MetIle: 0.917 ± 0.29
1.741MetLys: 1.741 ± 0.547
2.75MetLeu: 2.75 ± 0.466
0.183MetMet: 0.183 ± 0.131
0.733MetAsn: 0.733 ± 0.265
1.1MetPro: 1.1 ± 0.293
1.925MetGln: 1.925 ± 0.551
1.1MetArg: 1.1 ± 0.32
2.108MetSer: 2.108 ± 0.348
1.65MetThr: 1.65 ± 0.384
0.825MetVal: 0.825 ± 0.33
0.092MetTrp: 0.092 ± 0.087
0.55MetTyr: 0.55 ± 0.197
0.0MetXaa: 0.0 ± 0.0
Asn
4.033AsnAla: 4.033 ± 0.68
0.642AsnCys: 0.642 ± 0.367
3.483AsnAsp: 3.483 ± 0.6
2.383AsnGlu: 2.383 ± 0.458
1.833AsnPhe: 1.833 ± 0.399
4.124AsnGly: 4.124 ± 0.574
0.55AsnHis: 0.55 ± 0.246
3.483AsnIle: 3.483 ± 0.56
2.933AsnLys: 2.933 ± 0.437
4.949AsnLeu: 4.949 ± 0.731
0.458AsnMet: 0.458 ± 0.188
2.475AsnAsn: 2.475 ± 0.856
2.291AsnPro: 2.291 ± 0.339
1.741AsnGln: 1.741 ± 0.413
2.566AsnArg: 2.566 ± 0.528
3.116AsnSer: 3.116 ± 0.475
4.674AsnThr: 4.674 ± 0.762
3.666AsnVal: 3.666 ± 0.606
0.275AsnTrp: 0.275 ± 0.146
1.191AsnTyr: 1.191 ± 0.378
0.0AsnXaa: 0.0 ± 0.0
Pro
2.383ProAla: 2.383 ± 0.418
0.55ProCys: 0.55 ± 0.198
2.566ProAsp: 2.566 ± 0.364
2.933ProGlu: 2.933 ± 0.576
1.741ProPhe: 1.741 ± 0.442
1.466ProGly: 1.466 ± 0.37
0.55ProHis: 0.55 ± 0.227
2.108ProIle: 2.108 ± 0.483
2.933ProLys: 2.933 ± 0.486
3.483ProLeu: 3.483 ± 0.554
1.191ProMet: 1.191 ± 0.307
2.291ProAsn: 2.291 ± 0.39
2.108ProPro: 2.108 ± 0.565
1.191ProGln: 1.191 ± 0.28
1.741ProArg: 1.741 ± 0.449
3.941ProSer: 3.941 ± 0.567
2.475ProThr: 2.475 ± 0.511
2.658ProVal: 2.658 ± 0.378
0.458ProTrp: 0.458 ± 0.222
1.375ProTyr: 1.375 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
3.299GlnAla: 3.299 ± 0.723
0.458GlnCys: 0.458 ± 0.175
2.2GlnAsp: 2.2 ± 0.567
3.116GlnGlu: 3.116 ± 0.612
1.65GlnPhe: 1.65 ± 0.451
2.75GlnGly: 2.75 ± 0.518
0.825GlnHis: 0.825 ± 0.268
1.1GlnIle: 1.1 ± 0.276
2.2GlnLys: 2.2 ± 0.529
2.658GlnLeu: 2.658 ± 0.668
0.733GlnMet: 0.733 ± 0.3
1.1GlnAsn: 1.1 ± 0.273
1.558GlnPro: 1.558 ± 0.419
2.75GlnGln: 2.75 ± 0.741
2.933GlnArg: 2.933 ± 0.631
2.383GlnSer: 2.383 ± 0.453
2.2GlnThr: 2.2 ± 0.581
4.308GlnVal: 4.308 ± 0.602
0.642GlnTrp: 0.642 ± 0.239
0.642GlnTyr: 0.642 ± 0.28
0.0GlnXaa: 0.0 ± 0.0
Arg
4.033ArgAla: 4.033 ± 0.526
0.55ArgCys: 0.55 ± 0.302
3.758ArgAsp: 3.758 ± 0.825
2.75ArgGlu: 2.75 ± 0.597
2.383ArgPhe: 2.383 ± 0.443
4.674ArgGly: 4.674 ± 0.735
1.191ArgHis: 1.191 ± 0.289
2.566ArgIle: 2.566 ± 0.421
3.574ArgLys: 3.574 ± 0.832
5.407ArgLeu: 5.407 ± 0.646
1.466ArgMet: 1.466 ± 0.455
2.291ArgAsn: 2.291 ± 0.442
1.833ArgPro: 1.833 ± 0.391
1.375ArgGln: 1.375 ± 0.533
2.383ArgArg: 2.383 ± 0.575
2.475ArgSer: 2.475 ± 0.428
2.2ArgThr: 2.2 ± 0.491
2.383ArgVal: 2.383 ± 0.351
0.642ArgTrp: 0.642 ± 0.28
1.925ArgTyr: 1.925 ± 0.307
0.0ArgXaa: 0.0 ± 0.0
Ser
5.682SerAla: 5.682 ± 1.051
0.825SerCys: 0.825 ± 0.233
4.949SerAsp: 4.949 ± 0.609
3.941SerGlu: 3.941 ± 0.698
2.383SerPhe: 2.383 ± 0.611
7.332SerGly: 7.332 ± 1.648
1.191SerHis: 1.191 ± 0.34
4.124SerIle: 4.124 ± 0.654
4.033SerLys: 4.033 ± 0.637
6.232SerLeu: 6.232 ± 1.144
1.008SerMet: 1.008 ± 0.294
2.75SerAsn: 2.75 ± 0.529
1.925SerPro: 1.925 ± 0.377
3.116SerGln: 3.116 ± 0.385
3.483SerArg: 3.483 ± 0.625
5.224SerSer: 5.224 ± 1.385
3.391SerThr: 3.391 ± 0.769
4.491SerVal: 4.491 ± 0.948
1.283SerTrp: 1.283 ± 0.298
3.483SerTyr: 3.483 ± 0.475
0.0SerXaa: 0.0 ± 0.0
Thr
6.232ThrAla: 6.232 ± 0.963
0.642ThrCys: 0.642 ± 0.279
3.941ThrAsp: 3.941 ± 0.665
4.124ThrGlu: 4.124 ± 0.831
2.933ThrPhe: 2.933 ± 0.507
5.774ThrGly: 5.774 ± 1.021
1.008ThrHis: 1.008 ± 0.313
3.483ThrIle: 3.483 ± 0.73
3.483ThrLys: 3.483 ± 0.586
6.049ThrLeu: 6.049 ± 0.978
1.65ThrMet: 1.65 ± 0.365
3.391ThrAsn: 3.391 ± 0.735
4.033ThrPro: 4.033 ± 0.392
2.933ThrGln: 2.933 ± 0.528
2.933ThrArg: 2.933 ± 0.75
5.682ThrSer: 5.682 ± 1.642
6.507ThrThr: 6.507 ± 1.69
4.216ThrVal: 4.216 ± 0.93
1.375ThrTrp: 1.375 ± 0.354
2.016ThrTyr: 2.016 ± 0.447
0.0ThrXaa: 0.0 ± 0.0
Val
4.949ValAla: 4.949 ± 0.706
0.458ValCys: 0.458 ± 0.165
3.299ValAsp: 3.299 ± 0.413
4.399ValGlu: 4.399 ± 0.653
2.75ValPhe: 2.75 ± 0.395
3.574ValGly: 3.574 ± 0.627
1.1ValHis: 1.1 ± 0.244
3.666ValIle: 3.666 ± 0.739
3.299ValLys: 3.299 ± 0.541
4.491ValLeu: 4.491 ± 0.641
1.375ValMet: 1.375 ± 0.374
5.682ValAsn: 5.682 ± 0.754
1.65ValPro: 1.65 ± 0.396
1.925ValGln: 1.925 ± 0.475
1.925ValArg: 1.925 ± 0.523
4.491ValSer: 4.491 ± 0.772
6.507ValThr: 6.507 ± 1.512
4.583ValVal: 4.583 ± 0.674
0.458ValTrp: 0.458 ± 0.201
1.925ValTyr: 1.925 ± 0.618
0.0ValXaa: 0.0 ± 0.0
Trp
1.558TrpAla: 1.558 ± 0.41
0.275TrpCys: 0.275 ± 0.228
0.917TrpAsp: 0.917 ± 0.21
0.825TrpGlu: 0.825 ± 0.319
0.642TrpPhe: 0.642 ± 0.301
0.55TrpGly: 0.55 ± 0.197
0.458TrpHis: 0.458 ± 0.19
0.642TrpIle: 0.642 ± 0.239
0.55TrpLys: 0.55 ± 0.232
1.466TrpLeu: 1.466 ± 0.377
0.458TrpMet: 0.458 ± 0.232
0.458TrpAsn: 0.458 ± 0.287
0.55TrpPro: 0.55 ± 0.234
1.008TrpGln: 1.008 ± 0.382
0.55TrpArg: 0.55 ± 0.216
0.642TrpSer: 0.642 ± 0.392
0.733TrpThr: 0.733 ± 0.358
0.642TrpVal: 0.642 ± 0.231
0.092TrpTrp: 0.092 ± 0.097
0.367TrpTyr: 0.367 ± 0.199
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.933TyrAla: 2.933 ± 0.376
0.183TyrCys: 0.183 ± 0.137
2.383TyrAsp: 2.383 ± 0.5
2.2TyrGlu: 2.2 ± 0.5
1.375TyrPhe: 1.375 ± 0.295
1.741TyrGly: 1.741 ± 0.413
0.275TyrHis: 0.275 ± 0.157
1.65TyrIle: 1.65 ± 0.47
1.741TyrLys: 1.741 ± 0.364
2.475TyrLeu: 2.475 ± 0.483
0.367TyrMet: 0.367 ± 0.191
2.2TyrAsn: 2.2 ± 0.361
1.191TyrPro: 1.191 ± 0.408
0.917TyrGln: 0.917 ± 0.261
2.016TyrArg: 2.016 ± 0.396
2.016TyrSer: 2.016 ± 0.465
2.933TyrThr: 2.933 ± 0.499
1.65TyrVal: 1.65 ± 0.375
0.183TyrTrp: 0.183 ± 0.122
1.375TyrTyr: 1.375 ± 0.377
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 39 proteins (10912 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski