Blastomyces gilchristii (strain SLH14081) (Blastomyces dermatitidis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae; Blastomyces; Blastomyces gilchristii

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10303 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A179U624|A0A179U624_BLAGS Uncharacterized protein OS=Blastomyces gilchristii (strain SLH14081) OX=559298 GN=BDBG_16058 PE=4 SV=1
DDD2 pKa = 4.1YYY4 pKa = 11.43DD5 pKa = 4.19EE6 pKa = 5.84DD7 pKa = 4.05LEEE10 pKa = 4.25EEE12 pKa = 5.59EEE14 pKa = 4.07EEE16 pKa = 6.56DD17 pKa = 3.84DD18 pKa = 3.91TLHHH22 pKa = 6.81EE23 pKa = 4.4YY24 pKa = 11.08DD25 pKa = 3.77FKKK28 pKa = 10.83LDDD31 pKa = 3.82DDD33 pKa = 3.33CLLHHH38 pKa = 6.64DD39 pKa = 5.24ILYYY43 pKa = 10.03HH44 pKa = 5.08YY45 pKa = 9.21EEE47 pKa = 4.45LNSRR51 pKa = 11.84FSDDD55 pKa = 3.49LLINLDDD62 pKa = 4.34DD63 pKa = 6.29KK64 pKa = 11.91DD65 pKa = 4.36DD66 pKa = 3.76DDD68 pKa = 3.45KK69 pKa = 11.28YYY71 pKa = 11.36IMILSITLQNLAEEE85 pKa = 4.35QDDD88 pKa = 4.22STSIIQAVSTGVAAYYY104 pKa = 8.61INT

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A179UEN8|A0A179UEN8_BLAGS Uncharacterized protein OS=Blastomyces gilchristii (strain SLH14081) OX=559298 GN=BDBG_16476 PE=4 SV=1
MM1 pKa = 7.67ACLHH5 pKa = 6.03CTRR8 pKa = 11.84APVSLFSARR17 pKa = 11.84SLFEE21 pKa = 3.93SLPRR25 pKa = 11.84RR26 pKa = 11.84PASSLVIKK34 pKa = 9.44PQSQSSRR41 pKa = 11.84SQSRR45 pKa = 11.84AFSHH49 pKa = 6.78LFSKK53 pKa = 10.4RR54 pKa = 11.84QPTAATSTTSSSQPSILHH72 pKa = 6.52RR73 pKa = 11.84LLPSPATTTAFAQPTSATTAGLPLLSLSIPSFPTRR108 pKa = 11.84PFSASAALGAKK119 pKa = 9.25RR120 pKa = 11.84VTFNPSRR127 pKa = 11.84RR128 pKa = 11.84VQKK131 pKa = 10.2RR132 pKa = 11.84RR133 pKa = 11.84HH134 pKa = 5.34GFLARR139 pKa = 11.84LKK141 pKa = 8.13TQSGRR146 pKa = 11.84KK147 pKa = 8.19ILARR151 pKa = 11.84RR152 pKa = 11.84RR153 pKa = 11.84ARR155 pKa = 11.84GRR157 pKa = 11.84KK158 pKa = 7.83YY159 pKa = 10.92LSWW162 pKa = 5.23

Molecular weight: 17.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9690

613

10303

4560573

31

5916

442.6

49.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.949 ± 0.024

1.234 ± 0.009

5.565 ± 0.019

6.238 ± 0.024

3.629 ± 0.013

6.707 ± 0.024

2.493 ± 0.012

5.058 ± 0.015

5.014 ± 0.023

8.784 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.112 ± 0.01

3.913 ± 0.014

6.326 ± 0.025

4.085 ± 0.019

6.417 ± 0.02

8.807 ± 0.032

5.968 ± 0.016

5.732 ± 0.016

1.3 ± 0.009

2.669 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski