Anaerosporobacter mobilis DSM 15930
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4544 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M7NAT2|A0A1M7NAT2_9FIRM Uncharacterized protein OS=Anaerosporobacter mobilis DSM 15930 OX=1120996 GN=SAMN02746066_04342 PE=4 SV=1
MM1 pKa = 7.35 EE2 pKa = 6.5 KK3 pKa = 10.04 IRR5 pKa = 11.84 IKK7 pKa = 10.4 ATGKK11 pKa = 10.5 VYY13 pKa = 10.66 EE14 pKa = 4.92 LGNKK18 pKa = 8.24 KK19 pKa = 9.73 QAVEE23 pKa = 4.27 ARR25 pKa = 11.84 CSSLSGYY32 pKa = 9.78 VATNSDD38 pKa = 3.67 YY39 pKa = 11.12 EE40 pKa = 4.08 AAEE43 pKa = 4.01 EE44 pKa = 4.24 VIEE47 pKa = 5.13 KK48 pKa = 10.47 YY49 pKa = 10.03 RR50 pKa = 11.84 EE51 pKa = 3.87 SDD53 pKa = 2.87 NDD55 pKa = 4.09 RR56 pKa = 11.84 IFAEE60 pKa = 4.31 NDD62 pKa = 3.52 WVWTYY67 pKa = 10.62 NFKK70 pKa = 11.29 DD71 pKa = 3.6 EE72 pKa = 4.61 FDD74 pKa = 3.53 QAEE77 pKa = 4.15 EE78 pKa = 4.08 NMEE81 pKa = 3.98 EE82 pKa = 4.62 RR83 pKa = 11.84 YY84 pKa = 10.72 GDD86 pKa = 3.97 LAEE89 pKa = 4.8 HH90 pKa = 6.35 AQDD93 pKa = 5.85 LINKK97 pKa = 8.5 YY98 pKa = 10.4 DD99 pKa = 3.8 EE100 pKa = 4.55 SALMTGLDD108 pKa = 3.41 GCFYY112 pKa = 11.02 CVDD115 pKa = 3.56 TDD117 pKa = 3.79 EE118 pKa = 5.88 LYY120 pKa = 10.86 EE121 pKa = 4.92 DD122 pKa = 4.36 SLDD125 pKa = 3.88 EE126 pKa = 4.22 EE127 pKa = 4.58 ALGDD131 pKa = 5.09 FADD134 pKa = 4.11 EE135 pKa = 4.85 DD136 pKa = 4.78 DD137 pKa = 3.7 NLFYY141 pKa = 11.17 YY142 pKa = 8.79 VIPEE146 pKa = 3.75 IKK148 pKa = 9.96 FYY150 pKa = 11.05 LPEE153 pKa = 3.86 QVEE156 pKa = 4.73 VFSEE160 pKa = 4.18
Molecular weight: 18.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.923
IPC_protein 3.897
Toseland 3.706
ProMoST 3.999
Dawson 3.859
Bjellqvist 4.037
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.617
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.732
EMBOSS 3.757
Sillero 4.012
Patrickios 1.875
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.908
Protein with the highest isoelectric point:
>tr|A0A1M7IT05|A0A1M7IT05_9FIRM Ribosome-associated protein OS=Anaerosporobacter mobilis DSM 15930 OX=1120996 GN=SAMN02746066_01935 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.86 KK9 pKa = 8.2 RR10 pKa = 11.84 SRR12 pKa = 11.84 AKK14 pKa = 9.15 VHH16 pKa = 5.89 GFRR19 pKa = 11.84 KK20 pKa = 10.02 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 9.03 VIASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.21 GRR39 pKa = 11.84 HH40 pKa = 5.5 KK41 pKa = 10.9 LSAA44 pKa = 3.8
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.676
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.349
Sillero 12.866
Patrickios 12.398
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4544
0
4544
1495280
39
7383
329.1
37.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.188 ± 0.036
1.337 ± 0.015
5.69 ± 0.025
7.483 ± 0.048
4.095 ± 0.026
6.348 ± 0.037
1.591 ± 0.015
8.545 ± 0.044
7.527 ± 0.031
8.775 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.853 ± 0.018
5.497 ± 0.032
2.84 ± 0.021
3.156 ± 0.02
3.688 ± 0.027
6.273 ± 0.034
5.871 ± 0.04
6.821 ± 0.03
0.854 ± 0.013
4.566 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here