Gaeumannomyces tritici (strain R3-111a-1) (Wheat and barley take-all root rot fungus) (Gaeumannomyces graminis var. tritici)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Magnaporthales; Magnaporthaceae; Gaeumannomyces; Gaeumannomyces tritici

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14597 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J3NV87|J3NV87_GAET3 Metacaspase-1 OS=Gaeumannomyces tritici (strain R3-111a-1) OX=644352 GN=20345658 PE=3 SV=1
MM1 pKa = 7.07NRR3 pKa = 11.84AGGSRR8 pKa = 11.84RR9 pKa = 11.84ACVAGDD15 pKa = 3.8FEE17 pKa = 4.65QCLHH21 pKa = 5.85VVFEE25 pKa = 4.57RR26 pKa = 11.84PRR28 pKa = 11.84PCLFRR33 pKa = 11.84GSEE36 pKa = 4.35LMCQPTVVEE45 pKa = 5.4DD46 pKa = 3.97LLDD49 pKa = 3.41VCGRR53 pKa = 11.84CRR55 pKa = 11.84TWPGSYY61 pKa = 9.72EE62 pKa = 4.12LWRR65 pKa = 11.84APPLPPGWDD74 pKa = 3.4EE75 pKa = 4.62DD76 pKa = 4.52LSDD79 pKa = 3.72GTVPDD84 pKa = 3.94GDD86 pKa = 4.44YY87 pKa = 10.83EE88 pKa = 4.45DD89 pKa = 4.72SSSEE93 pKa = 3.95EE94 pKa = 4.36SPCSSVLSTVFSLSDD109 pKa = 3.3EE110 pKa = 4.53CSTGDD115 pKa = 3.37TDD117 pKa = 3.82SCSDD121 pKa = 3.69YY122 pKa = 11.41EE123 pKa = 4.6EE124 pKa = 4.82EE125 pKa = 6.32DD126 pKa = 3.61GDD128 pKa = 3.9GWKK131 pKa = 10.97GGDD134 pKa = 3.45DD135 pKa = 3.66AGEE138 pKa = 4.45GEE140 pKa = 4.49YY141 pKa = 10.99SLTRR145 pKa = 11.84RR146 pKa = 11.84DD147 pKa = 3.33TCLLPGG153 pKa = 3.93

Molecular weight: 16.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J3PCI5|J3PCI5_GAET3 ATP-dependent DNA helicase OS=Gaeumannomyces tritici (strain R3-111a-1) OX=644352 GN=20351664 PE=3 SV=1
MM1 pKa = 7.48PVFRR5 pKa = 11.84RR6 pKa = 11.84ARR8 pKa = 11.84GRR10 pKa = 11.84KK11 pKa = 8.34VMVRR15 pKa = 11.84ILLAKK20 pKa = 8.47TRR22 pKa = 11.84KK23 pKa = 10.27AMDD26 pKa = 3.28QGSASHH32 pKa = 6.72RR33 pKa = 11.84GHH35 pKa = 6.45RR36 pKa = 11.84HH37 pKa = 4.49GLVSQRR43 pKa = 11.84QLKK46 pKa = 7.63STRR49 pKa = 11.84GPARR53 pKa = 11.84EE54 pKa = 3.88VWSRR58 pKa = 11.84WGSRR62 pKa = 11.84QGRR65 pKa = 11.84SSNVMLRR72 pKa = 11.84GPFSGG77 pKa = 4.16

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14456

141

14597

6286680

32

6633

430.7

46.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.47 ± 0.024

1.314 ± 0.009

5.69 ± 0.015

5.63 ± 0.021

3.361 ± 0.014

7.987 ± 0.023

2.383 ± 0.01

3.891 ± 0.013

4.222 ± 0.019

8.64 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.14 ± 0.008

3.078 ± 0.013

6.741 ± 0.028

3.914 ± 0.021

6.988 ± 0.018

7.905 ± 0.024

5.563 ± 0.016

6.272 ± 0.018

1.461 ± 0.009

2.349 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski