Escherichia phage EC6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Felixounavirus; Escherichia virus EC6

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 136 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4IC09|K4IC09_9CAUD Uncharacterized protein OS=Escherichia phage EC6 OX=1229757 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.8CFHH5 pKa = 6.61GTTQEE10 pKa = 3.86NFTNLINNGDD20 pKa = 4.02KK21 pKa = 10.86PSGAWNCSDD30 pKa = 3.4MDD32 pKa = 4.16GNFYY36 pKa = 10.85VYY38 pKa = 10.1PEE40 pKa = 3.92NKK42 pKa = 9.59IYY44 pKa = 10.85GDD46 pKa = 3.86DD47 pKa = 3.75AEE49 pKa = 5.07EE50 pKa = 4.05IASEE54 pKa = 5.16GIQQALGNATITAAFQMKK72 pKa = 7.87TQNIVILEE80 pKa = 4.02LDD82 pKa = 3.41IPEE85 pKa = 5.73DD86 pKa = 4.11EE87 pKa = 5.46LNDD90 pKa = 5.32DD91 pKa = 4.11YY92 pKa = 12.13SCDD95 pKa = 3.52NMSGVASFTEE105 pKa = 4.28YY106 pKa = 11.01FDD108 pKa = 5.33LNWIKK113 pKa = 10.55KK114 pKa = 9.85VYY116 pKa = 7.05TTEE119 pKa = 3.93FNAMFSPFCLPSLDD133 pKa = 4.3NPNLNYY139 pKa = 9.49IDD141 pKa = 4.36EE142 pKa = 4.48SLEE145 pKa = 3.85LLAKK149 pKa = 10.14SVQQSDD155 pKa = 4.51SIQVFCDD162 pKa = 2.88IMDD165 pKa = 4.27TLTEE169 pKa = 4.29NIIEE173 pKa = 4.4RR174 pKa = 11.84DD175 pKa = 3.28LKK177 pKa = 11.43GFFF180 pKa = 4.04

Molecular weight: 20.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4I142|K4I142_9CAUD Uncharacterized protein OS=Escherichia phage EC6 OX=1229757 PE=4 SV=1
MM1 pKa = 7.79GILGNMKK8 pKa = 10.48AGFLKK13 pKa = 10.81ALAKK17 pKa = 10.02AAAIIAMTGKK27 pKa = 9.93QVGVDD32 pKa = 3.61ASAIAQVLASQIEE45 pKa = 4.18QQPYY49 pKa = 7.88IHH51 pKa = 6.62VGRR54 pKa = 11.84GKK56 pKa = 10.52GGKK59 pKa = 8.65KK60 pKa = 8.53QAHH63 pKa = 6.49RR64 pKa = 11.84PTGAAAIKK72 pKa = 10.06RR73 pKa = 11.84AAKK76 pKa = 9.81KK77 pKa = 10.5ASNHH81 pKa = 5.17KK82 pKa = 10.06RR83 pKa = 11.84NKK85 pKa = 8.42KK86 pKa = 10.18AKK88 pKa = 9.42

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

136

0

136

24739

38

747

181.9

20.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.021 ± 0.303

1.415 ± 0.118

6.273 ± 0.187

6.928 ± 0.277

4.273 ± 0.119

6.419 ± 0.157

1.989 ± 0.145

6.116 ± 0.156

7.858 ± 0.273

7.773 ± 0.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.89 ± 0.134

5.283 ± 0.2

2.947 ± 0.141

3.48 ± 0.171

4.066 ± 0.132

6.439 ± 0.222

6.225 ± 0.256

6.945 ± 0.161

1.18 ± 0.09

4.475 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski