Amino acid dipepetide frequency for Escherichia phage EC6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.134AlaAla: 5.134 ± 0.668
0.647AlaCys: 0.647 ± 0.153
4.042AlaAsp: 4.042 ± 0.425
4.244AlaGlu: 4.244 ± 0.378
2.708AlaPhe: 2.708 ± 0.334
4.81AlaGly: 4.81 ± 0.501
1.496AlaHis: 1.496 ± 0.313
4.851AlaIle: 4.851 ± 0.401
5.295AlaLys: 5.295 ± 0.486
5.781AlaLeu: 5.781 ± 0.504
2.708AlaMet: 2.708 ± 0.338
3.8AlaAsn: 3.8 ± 0.454
1.779AlaPro: 1.779 ± 0.289
2.587AlaGln: 2.587 ± 0.4
2.345AlaArg: 2.345 ± 0.322
4.527AlaSer: 4.527 ± 0.521
4.406AlaThr: 4.406 ± 0.472
5.013AlaVal: 5.013 ± 0.452
0.97AlaTrp: 0.97 ± 0.193
3.072AlaTyr: 3.072 ± 0.356
0.0AlaXaa: 0.0 ± 0.0
Cys
0.485CysAla: 0.485 ± 0.174
0.202CysCys: 0.202 ± 0.107
0.566CysAsp: 0.566 ± 0.141
1.051CysGlu: 1.051 ± 0.225
0.93CysPhe: 0.93 ± 0.173
1.091CysGly: 1.091 ± 0.247
0.526CysHis: 0.526 ± 0.142
0.566CysIle: 0.566 ± 0.127
1.334CysLys: 1.334 ± 0.236
0.889CysLeu: 0.889 ± 0.183
0.323CysMet: 0.323 ± 0.111
0.849CysAsn: 0.849 ± 0.183
0.606CysPro: 0.606 ± 0.172
0.526CysGln: 0.526 ± 0.177
0.606CysArg: 0.606 ± 0.178
1.172CysSer: 1.172 ± 0.262
0.687CysThr: 0.687 ± 0.143
0.889CysVal: 0.889 ± 0.174
0.121CysTrp: 0.121 ± 0.072
0.728CysTyr: 0.728 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
4.73AspAla: 4.73 ± 0.468
0.647AspCys: 0.647 ± 0.158
3.557AspAsp: 3.557 ± 0.399
4.447AspGlu: 4.447 ± 0.429
3.436AspPhe: 3.436 ± 0.337
5.053AspGly: 5.053 ± 0.466
0.728AspHis: 0.728 ± 0.192
4.366AspIle: 4.366 ± 0.451
4.487AspLys: 4.487 ± 0.368
5.174AspLeu: 5.174 ± 0.362
1.657AspMet: 1.657 ± 0.237
3.517AspAsn: 3.517 ± 0.385
1.738AspPro: 1.738 ± 0.29
0.606AspGln: 0.606 ± 0.159
1.9AspArg: 1.9 ± 0.236
4.204AspSer: 4.204 ± 0.382
3.921AspThr: 3.921 ± 0.417
4.487AspVal: 4.487 ± 0.39
1.334AspTrp: 1.334 ± 0.225
2.749AspTyr: 2.749 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
5.578GluAla: 5.578 ± 0.679
0.889GluCys: 0.889 ± 0.182
4.204GluAsp: 4.204 ± 0.457
5.821GluGlu: 5.821 ± 0.53
3.153GluPhe: 3.153 ± 0.41
4.244GluGly: 4.244 ± 0.361
1.415GluHis: 1.415 ± 0.258
4.204GluIle: 4.204 ± 0.451
5.174GluLys: 5.174 ± 0.486
6.144GluLeu: 6.144 ± 0.519
2.87GluMet: 2.87 ± 0.279
3.598GluAsn: 3.598 ± 0.362
1.577GluPro: 1.577 ± 0.223
2.385GluGln: 2.385 ± 0.339
2.991GluArg: 2.991 ± 0.326
3.921GluSer: 3.921 ± 0.337
3.517GluThr: 3.517 ± 0.355
4.568GluVal: 4.568 ± 0.422
0.647GluTrp: 0.647 ± 0.172
2.385GluTyr: 2.385 ± 0.326
0.0GluXaa: 0.0 ± 0.0
Phe
2.789PheAla: 2.789 ± 0.457
0.485PheCys: 0.485 ± 0.143
3.153PheAsp: 3.153 ± 0.322
3.234PheGlu: 3.234 ± 0.35
1.253PhePhe: 1.253 ± 0.19
3.153PheGly: 3.153 ± 0.386
0.93PheHis: 0.93 ± 0.199
2.587PheIle: 2.587 ± 0.276
3.759PheLys: 3.759 ± 0.369
2.708PheLeu: 2.708 ± 0.356
1.091PheMet: 1.091 ± 0.189
2.425PheAsn: 2.425 ± 0.284
0.97PhePro: 0.97 ± 0.184
1.455PheGln: 1.455 ± 0.259
1.496PheArg: 1.496 ± 0.208
2.991PheSer: 2.991 ± 0.316
2.87PheThr: 2.87 ± 0.296
2.708PheVal: 2.708 ± 0.317
0.364PheTrp: 0.364 ± 0.112
2.304PheTyr: 2.304 ± 0.33
0.0PheXaa: 0.0 ± 0.0
Gly
4.972GlyAla: 4.972 ± 0.627
1.091GlyCys: 1.091 ± 0.232
3.274GlyAsp: 3.274 ± 0.321
4.285GlyGlu: 4.285 ± 0.412
3.274GlyPhe: 3.274 ± 0.329
4.244GlyGly: 4.244 ± 0.42
1.415GlyHis: 1.415 ± 0.201
4.527GlyIle: 4.527 ± 0.414
6.266GlyLys: 6.266 ± 0.53
4.689GlyLeu: 4.689 ± 0.389
1.091GlyMet: 1.091 ± 0.212
3.153GlyAsn: 3.153 ± 0.406
0.081GlyPro: 0.081 ± 0.055
2.223GlyGln: 2.223 ± 0.326
2.385GlyArg: 2.385 ± 0.285
4.204GlySer: 4.204 ± 0.465
3.679GlyThr: 3.679 ± 0.51
5.295GlyVal: 5.295 ± 0.393
0.93GlyTrp: 0.93 ± 0.186
3.113GlyTyr: 3.113 ± 0.324
0.0GlyXaa: 0.0 ± 0.0
His
1.011HisAla: 1.011 ± 0.189
0.526HisCys: 0.526 ± 0.137
1.051HisAsp: 1.051 ± 0.202
1.213HisGlu: 1.213 ± 0.204
0.808HisPhe: 0.808 ± 0.187
0.889HisGly: 0.889 ± 0.178
0.445HisHis: 0.445 ± 0.156
1.132HisIle: 1.132 ± 0.211
1.536HisLys: 1.536 ± 0.305
1.738HisLeu: 1.738 ± 0.249
0.687HisMet: 0.687 ± 0.152
1.011HisAsn: 1.011 ± 0.222
0.808HisPro: 0.808 ± 0.159
0.445HisGln: 0.445 ± 0.112
0.808HisArg: 0.808 ± 0.153
1.536HisSer: 1.536 ± 0.373
1.415HisThr: 1.415 ± 0.408
1.415HisVal: 1.415 ± 0.287
0.283HisTrp: 0.283 ± 0.145
1.132HisTyr: 1.132 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
4.042IleAla: 4.042 ± 0.327
1.091IleCys: 1.091 ± 0.207
4.487IleAsp: 4.487 ± 0.437
4.042IleGlu: 4.042 ± 0.433
2.466IlePhe: 2.466 ± 0.302
3.113IleGly: 3.113 ± 0.361
1.132IleHis: 1.132 ± 0.196
3.719IleIle: 3.719 ± 0.452
5.498IleLys: 5.498 ± 0.54
3.962IleLeu: 3.962 ± 0.402
1.738IleMet: 1.738 ± 0.238
3.274IleAsn: 3.274 ± 0.379
2.102IlePro: 2.102 ± 0.291
1.9IleGln: 1.9 ± 0.253
2.911IleArg: 2.911 ± 0.302
4.123IleSer: 4.123 ± 0.429
4.123IleThr: 4.123 ± 0.422
3.881IleVal: 3.881 ± 0.37
0.647IleTrp: 0.647 ± 0.148
2.911IleTyr: 2.911 ± 0.316
0.0IleXaa: 0.0 ± 0.0
Lys
5.983LysAla: 5.983 ± 0.566
1.011LysCys: 1.011 ± 0.217
4.689LysAsp: 4.689 ± 0.402
6.832LysGlu: 6.832 ± 0.637
2.587LysPhe: 2.587 ± 0.361
5.174LysGly: 5.174 ± 0.523
1.374LysHis: 1.374 ± 0.247
4.689LysIle: 4.689 ± 0.447
5.457LysLys: 5.457 ± 0.61
5.821LysLeu: 5.821 ± 0.489
2.466LysMet: 2.466 ± 0.286
3.962LysAsn: 3.962 ± 0.332
2.547LysPro: 2.547 ± 0.307
2.951LysGln: 2.951 ± 0.332
3.638LysArg: 3.638 ± 0.346
4.285LysSer: 4.285 ± 0.332
5.295LysThr: 5.295 ± 0.5
6.751LysVal: 6.751 ± 0.536
0.566LysTrp: 0.566 ± 0.16
2.506LysTyr: 2.506 ± 0.442
0.0LysXaa: 0.0 ± 0.0
Leu
5.7LeuAla: 5.7 ± 0.419
1.011LeuCys: 1.011 ± 0.198
5.781LeuAsp: 5.781 ± 0.485
6.347LeuGlu: 6.347 ± 0.528
2.668LeuPhe: 2.668 ± 0.332
3.881LeuGly: 3.881 ± 0.333
1.657LeuHis: 1.657 ± 0.325
3.476LeuIle: 3.476 ± 0.327
6.791LeuLys: 6.791 ± 0.536
5.578LeuLeu: 5.578 ± 0.395
2.587LeuMet: 2.587 ± 0.373
4.204LeuAsn: 4.204 ± 0.374
3.153LeuPro: 3.153 ± 0.341
3.193LeuGln: 3.193 ± 0.368
3.679LeuArg: 3.679 ± 0.356
5.093LeuSer: 5.093 ± 0.389
4.77LeuThr: 4.77 ± 0.475
4.083LeuVal: 4.083 ± 0.478
0.889LeuTrp: 0.889 ± 0.177
3.153LeuTyr: 3.153 ± 0.374
0.04LeuXaa: 0.04 ± 0.033
Met
2.264MetAla: 2.264 ± 0.272
0.323MetCys: 0.323 ± 0.129
1.334MetAsp: 1.334 ± 0.235
1.657MetGlu: 1.657 ± 0.231
1.577MetPhe: 1.577 ± 0.235
1.738MetGly: 1.738 ± 0.223
0.323MetHis: 0.323 ± 0.126
1.819MetIle: 1.819 ± 0.252
2.749MetLys: 2.749 ± 0.296
2.506MetLeu: 2.506 ± 0.345
0.768MetMet: 0.768 ± 0.181
1.536MetAsn: 1.536 ± 0.23
0.687MetPro: 0.687 ± 0.18
1.577MetGln: 1.577 ± 0.25
1.577MetArg: 1.577 ± 0.199
2.062MetSer: 2.062 ± 0.292
1.981MetThr: 1.981 ± 0.261
1.294MetVal: 1.294 ± 0.219
0.243MetTrp: 0.243 ± 0.099
0.889MetTyr: 0.889 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
3.638AsnAla: 3.638 ± 0.38
0.889AsnCys: 0.889 ± 0.185
2.83AsnAsp: 2.83 ± 0.316
2.385AsnGlu: 2.385 ± 0.276
2.345AsnPhe: 2.345 ± 0.282
4.406AsnGly: 4.406 ± 0.399
1.455AsnHis: 1.455 ± 0.268
3.679AsnIle: 3.679 ± 0.441
3.8AsnLys: 3.8 ± 0.45
4.608AsnLeu: 4.608 ± 0.507
1.213AsnMet: 1.213 ± 0.248
3.234AsnAsn: 3.234 ± 0.38
2.062AsnPro: 2.062 ± 0.258
1.617AsnGln: 1.617 ± 0.274
2.102AsnArg: 2.102 ± 0.289
3.598AsnSer: 3.598 ± 0.407
2.628AsnThr: 2.628 ± 0.361
3.193AsnVal: 3.193 ± 0.314
0.728AsnTrp: 0.728 ± 0.159
2.385AsnTyr: 2.385 ± 0.348
0.0AsnXaa: 0.0 ± 0.0
Pro
1.859ProAla: 1.859 ± 0.279
0.364ProCys: 0.364 ± 0.117
2.304ProAsp: 2.304 ± 0.398
3.072ProGlu: 3.072 ± 0.339
1.698ProPhe: 1.698 ± 0.309
0.202ProGly: 0.202 ± 0.088
0.606ProHis: 0.606 ± 0.202
1.334ProIle: 1.334 ± 0.196
2.223ProLys: 2.223 ± 0.302
2.102ProLeu: 2.102 ± 0.256
1.051ProMet: 1.051 ± 0.21
1.415ProAsn: 1.415 ± 0.25
0.93ProPro: 0.93 ± 0.211
1.051ProGln: 1.051 ± 0.22
0.97ProArg: 0.97 ± 0.201
2.062ProSer: 2.062 ± 0.311
2.425ProThr: 2.425 ± 0.297
2.304ProVal: 2.304 ± 0.29
0.162ProTrp: 0.162 ± 0.081
1.334ProTyr: 1.334 ± 0.235
0.0ProXaa: 0.0 ± 0.0
Gln
2.385GlnAla: 2.385 ± 0.325
0.526GlnCys: 0.526 ± 0.149
1.698GlnAsp: 1.698 ± 0.266
2.587GlnGlu: 2.587 ± 0.32
1.577GlnPhe: 1.577 ± 0.222
1.819GlnGly: 1.819 ± 0.269
0.566GlnHis: 0.566 ± 0.152
2.506GlnIle: 2.506 ± 0.318
2.506GlnLys: 2.506 ± 0.318
2.668GlnLeu: 2.668 ± 0.384
1.091GlnMet: 1.091 ± 0.248
1.657GlnAsn: 1.657 ± 0.273
1.132GlnPro: 1.132 ± 0.186
1.536GlnGln: 1.536 ± 0.245
1.617GlnArg: 1.617 ± 0.252
1.819GlnSer: 1.819 ± 0.231
1.859GlnThr: 1.859 ± 0.247
2.708GlnVal: 2.708 ± 0.334
0.526GlnTrp: 0.526 ± 0.156
2.021GlnTyr: 2.021 ± 0.249
0.0GlnXaa: 0.0 ± 0.0
Arg
2.425ArgAla: 2.425 ± 0.293
0.97ArgCys: 0.97 ± 0.225
3.032ArgAsp: 3.032 ± 0.34
2.425ArgGlu: 2.425 ± 0.338
1.334ArgPhe: 1.334 ± 0.269
2.749ArgGly: 2.749 ± 0.398
0.97ArgHis: 0.97 ± 0.184
2.749ArgIle: 2.749 ± 0.25
2.708ArgLys: 2.708 ± 0.325
3.436ArgLeu: 3.436 ± 0.405
1.294ArgMet: 1.294 ± 0.225
2.062ArgAsn: 2.062 ± 0.25
0.889ArgPro: 0.889 ± 0.175
1.617ArgGln: 1.617 ± 0.223
2.021ArgArg: 2.021 ± 0.286
2.142ArgSer: 2.142 ± 0.235
2.385ArgThr: 2.385 ± 0.336
2.789ArgVal: 2.789 ± 0.27
0.526ArgTrp: 0.526 ± 0.165
2.142ArgTyr: 2.142 ± 0.276
0.0ArgXaa: 0.0 ± 0.0
Ser
4.164SerAla: 4.164 ± 0.403
0.687SerCys: 0.687 ± 0.161
4.527SerAsp: 4.527 ± 0.437
4.366SerGlu: 4.366 ± 0.441
2.991SerPhe: 2.991 ± 0.368
3.476SerGly: 3.476 ± 0.428
1.213SerHis: 1.213 ± 0.292
3.962SerIle: 3.962 ± 0.38
4.204SerLys: 4.204 ± 0.452
5.821SerLeu: 5.821 ± 0.413
1.617SerMet: 1.617 ± 0.234
3.153SerAsn: 3.153 ± 0.313
1.657SerPro: 1.657 ± 0.255
2.789SerGln: 2.789 ± 0.364
2.587SerArg: 2.587 ± 0.323
4.81SerSer: 4.81 ± 0.473
3.315SerThr: 3.315 ± 0.507
5.134SerVal: 5.134 ± 0.396
0.93SerTrp: 0.93 ± 0.204
2.991SerTyr: 2.991 ± 0.287
0.0SerXaa: 0.0 ± 0.0
Thr
4.285ThrAla: 4.285 ± 0.505
0.485ThrCys: 0.485 ± 0.144
3.8ThrAsp: 3.8 ± 0.342
3.113ThrGlu: 3.113 ± 0.379
3.153ThrPhe: 3.153 ± 0.304
5.578ThrGly: 5.578 ± 0.43
1.294ThrHis: 1.294 ± 0.288
3.396ThrIle: 3.396 ± 0.395
4.73ThrLys: 4.73 ± 0.518
4.568ThrLeu: 4.568 ± 0.491
0.808ThrMet: 0.808 ± 0.165
2.668ThrAsn: 2.668 ± 0.327
2.385ThrPro: 2.385 ± 0.315
2.385ThrGln: 2.385 ± 0.266
2.345ThrArg: 2.345 ± 0.22
4.002ThrSer: 4.002 ± 0.437
3.84ThrThr: 3.84 ± 0.588
5.457ThrVal: 5.457 ± 0.49
0.728ThrTrp: 0.728 ± 0.206
3.234ThrTyr: 3.234 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
5.821ValAla: 5.821 ± 0.477
1.091ValCys: 1.091 ± 0.198
4.73ValAsp: 4.73 ± 0.406
4.244ValGlu: 4.244 ± 0.38
2.385ValPhe: 2.385 ± 0.316
4.81ValGly: 4.81 ± 0.527
1.172ValHis: 1.172 ± 0.236
4.447ValIle: 4.447 ± 0.447
6.064ValLys: 6.064 ± 0.47
4.447ValLeu: 4.447 ± 0.383
2.223ValMet: 2.223 ± 0.306
4.002ValAsn: 4.002 ± 0.473
2.425ValPro: 2.425 ± 0.371
1.819ValGln: 1.819 ± 0.26
2.628ValArg: 2.628 ± 0.313
4.447ValSer: 4.447 ± 0.449
4.649ValThr: 4.649 ± 0.429
4.366ValVal: 4.366 ± 0.442
0.647ValTrp: 0.647 ± 0.184
3.032ValTyr: 3.032 ± 0.333
0.0ValXaa: 0.0 ± 0.0
Trp
0.283TrpAla: 0.283 ± 0.123
0.162TrpCys: 0.162 ± 0.09
0.93TrpAsp: 0.93 ± 0.171
0.889TrpGlu: 0.889 ± 0.171
0.647TrpPhe: 0.647 ± 0.154
0.566TrpGly: 0.566 ± 0.129
0.081TrpHis: 0.081 ± 0.057
0.566TrpIle: 0.566 ± 0.171
0.889TrpLys: 0.889 ± 0.187
1.132TrpLeu: 1.132 ± 0.195
0.364TrpMet: 0.364 ± 0.132
0.93TrpAsn: 0.93 ± 0.196
0.283TrpPro: 0.283 ± 0.113
0.485TrpGln: 0.485 ± 0.133
0.445TrpArg: 0.445 ± 0.156
0.808TrpSer: 0.808 ± 0.167
0.768TrpThr: 0.768 ± 0.173
0.728TrpVal: 0.728 ± 0.158
0.162TrpTrp: 0.162 ± 0.069
0.687TrpTyr: 0.687 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.668TyrAla: 2.668 ± 0.381
1.051TyrCys: 1.051 ± 0.215
2.749TyrAsp: 2.749 ± 0.333
3.032TyrGlu: 3.032 ± 0.297
1.738TyrPhe: 1.738 ± 0.219
3.193TyrGly: 3.193 ± 0.337
1.091TyrHis: 1.091 ± 0.216
2.587TyrIle: 2.587 ± 0.298
3.072TyrLys: 3.072 ± 0.273
3.962TyrLeu: 3.962 ± 0.402
1.213TyrMet: 1.213 ± 0.187
2.385TyrAsn: 2.385 ± 0.308
1.657TyrPro: 1.657 ± 0.269
1.657TyrGln: 1.657 ± 0.234
1.617TyrArg: 1.617 ± 0.24
2.587TyrSer: 2.587 ± 0.305
3.719TyrThr: 3.719 ± 0.422
2.385TyrVal: 2.385 ± 0.309
0.404TyrTrp: 0.404 ± 0.127
1.981TyrTyr: 1.981 ± 0.3
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.04XaaLys: 0.04 ± 0.033
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 136 proteins (24739 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski