Oita virus
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R163|A0A0D3R163_9RHAB Nucleocapsid protein OS=Oita virus OX=1272953 PE=4 SV=1
MM1 pKa = 7.35 NSDD4 pKa = 3.24 RR5 pKa = 11.84 AKK7 pKa = 10.03 IQRR10 pKa = 11.84 LAKK13 pKa = 9.91 ALPWEE18 pKa = 4.04 NVKK21 pKa = 10.6 INIEE25 pKa = 4.38 DD26 pKa = 3.76 IEE28 pKa = 4.59 DD29 pKa = 3.71 SEE31 pKa = 5.02 EE32 pKa = 4.39 EE33 pKa = 3.81 IDD35 pKa = 3.54 QKK37 pKa = 11.16 IIVTEE42 pKa = 3.98 EE43 pKa = 3.98 RR44 pKa = 11.84 DD45 pKa = 4.17 LEE47 pKa = 4.34 WTEE50 pKa = 4.16 NPIKK54 pKa = 10.53 EE55 pKa = 4.13 QVEE58 pKa = 3.86 PFEE61 pKa = 4.22 IQEE64 pKa = 4.16 EE65 pKa = 4.52 DD66 pKa = 3.53 STPTEE71 pKa = 4.31 MGLSACEE78 pKa = 3.9 KK79 pKa = 9.56 EE80 pKa = 4.22 KK81 pKa = 11.09 EE82 pKa = 4.11 KK83 pKa = 11.16 EE84 pKa = 4.14 KK85 pKa = 11.28 EE86 pKa = 3.58 KK87 pKa = 10.67 DD88 pKa = 3.01 TAEE91 pKa = 3.99 NKK93 pKa = 10.46 GIEE96 pKa = 4.37 GSGQGCSKK104 pKa = 10.13 RR105 pKa = 11.84 SKK107 pKa = 9.98 RR108 pKa = 11.84 GKK110 pKa = 10.05 GEE112 pKa = 3.6 QRR114 pKa = 11.84 GKK116 pKa = 10.47 NKK118 pKa = 10.26 SEE120 pKa = 4.0 EE121 pKa = 3.99 TSAPSDD127 pKa = 3.28 SATITNPDD135 pKa = 3.07 IKK137 pKa = 10.39 EE138 pKa = 4.32 DD139 pKa = 3.95 PVCALSVEE147 pKa = 4.52 EE148 pKa = 3.98 DD149 pKa = 3.58 ALWRR153 pKa = 11.84 EE154 pKa = 4.59 EE155 pKa = 3.82 KK156 pKa = 10.47 RR157 pKa = 11.84 KK158 pKa = 9.46 EE159 pKa = 3.96 ALRR162 pKa = 11.84 LQSDD166 pKa = 4.87 AIRR169 pKa = 11.84 VEE171 pKa = 3.77 RR172 pKa = 11.84 VNYY175 pKa = 10.57 NEE177 pKa = 4.98 IKK179 pKa = 9.01 TQEE182 pKa = 4.31 DD183 pKa = 4.02 LDD185 pKa = 4.24 DD186 pKa = 5.45 LMTEE190 pKa = 4.36 VIYY193 pKa = 10.72 KK194 pKa = 10.16 VLEE197 pKa = 4.12 CTGYY201 pKa = 10.44 CARR204 pKa = 11.84 SDD206 pKa = 3.53 LTSIRR211 pKa = 11.84 RR212 pKa = 11.84 DD213 pKa = 3.92 DD214 pKa = 3.1 IRR216 pKa = 11.84 VYY218 pKa = 10.76 YY219 pKa = 10.37 KK220 pKa = 10.62 EE221 pKa = 4.55 SFVKK225 pKa = 9.91 EE226 pKa = 4.07 AEE228 pKa = 4.24 RR229 pKa = 11.84 QTEE232 pKa = 4.06 EE233 pKa = 3.4 WKK235 pKa = 10.68 KK236 pKa = 10.64 RR237 pKa = 11.84 LEE239 pKa = 4.0 EE240 pKa = 4.29 EE241 pKa = 4.95 IKK243 pKa = 11.07 DD244 pKa = 3.82 KK245 pKa = 10.59 TCKK248 pKa = 10.49 DD249 pKa = 3.75 NLCDD253 pKa = 4.01 PDD255 pKa = 4.14 SKK257 pKa = 11.1 RR258 pKa = 11.84 EE259 pKa = 3.92 EE260 pKa = 3.98 NKK262 pKa = 10.69 ANPKK266 pKa = 9.63 DD267 pKa = 3.82 QSSSSIKK274 pKa = 9.79 EE275 pKa = 3.53 RR276 pKa = 11.84 HH277 pKa = 4.8 QPQIEE282 pKa = 4.54 STDD285 pKa = 3.39 EE286 pKa = 3.92 PNPRR290 pKa = 11.84 KK291 pKa = 9.49 EE292 pKa = 4.08 DD293 pKa = 3.45 RR294 pKa = 11.84 SDD296 pKa = 3.37 NAEE299 pKa = 4.44 DD300 pKa = 3.34 STAIQMQLEE309 pKa = 4.3 TLLQEE314 pKa = 4.54 GMCFRR319 pKa = 11.84 KK320 pKa = 10.0 KK321 pKa = 10.07 RR322 pKa = 11.84 GGYY325 pKa = 6.59 MTVSHH330 pKa = 7.62 DD331 pKa = 3.64 NPKK334 pKa = 10.61 LKK336 pKa = 10.49 NIKK339 pKa = 9.93 LKK341 pKa = 10.71 EE342 pKa = 3.89 IALSSGSVKK351 pKa = 9.62 EE352 pKa = 4.33 AKK354 pKa = 9.91 ILLLKK359 pKa = 10.6 KK360 pKa = 10.26 AGLYY364 pKa = 10.0 QGIKK368 pKa = 9.3 LLCYY372 pKa = 9.89 IDD374 pKa = 3.71
Molecular weight: 43.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.007
IPC2_protein 4.978
IPC_protein 4.902
Toseland 4.762
ProMoST 4.94
Dawson 4.825
Bjellqvist 4.978
Wikipedia 4.647
Rodwell 4.736
Grimsley 4.673
Solomon 4.825
Lehninger 4.774
Nozaki 4.927
DTASelect 5.029
Thurlkill 4.736
EMBOSS 4.673
Sillero 5.003
Patrickios 4.368
IPC_peptide 4.825
IPC2_peptide 5.003
IPC2.peptide.svr19 4.964
Protein with the highest isoelectric point:
>tr|A0A0D3R1Q4|A0A0D3R1Q4_9RHAB Matrix OS=Oita virus OX=1272953 PE=4 SV=1
MM1 pKa = 7.47 LSVFKK6 pKa = 10.59 KK7 pKa = 9.77 SKK9 pKa = 9.68 KK10 pKa = 10.05 DD11 pKa = 3.5 RR12 pKa = 11.84 VSSSAPDD19 pKa = 3.32 TSQLVKK25 pKa = 9.71 EE26 pKa = 4.74 TFFSVPSAPAPSIKK40 pKa = 9.96 KK41 pKa = 10.42 DD42 pKa = 3.16 IFTHH46 pKa = 4.89 TLRR49 pKa = 11.84 VQAWIEE55 pKa = 3.8 IRR57 pKa = 11.84 SEE59 pKa = 3.77 EE60 pKa = 4.29 SIQDD64 pKa = 3.65 FEE66 pKa = 5.19 DD67 pKa = 3.48 CTKK70 pKa = 10.45 ILEE73 pKa = 4.14 PWIDD77 pKa = 3.74 EE78 pKa = 4.17 ASCPIWQYY86 pKa = 11.3 IIDD89 pKa = 3.8 SWVFFCMGVHH99 pKa = 6.26 ARR101 pKa = 11.84 KK102 pKa = 10.13 DD103 pKa = 3.77 PLCTTHH109 pKa = 6.75 CLYY112 pKa = 10.51 RR113 pKa = 11.84 ARR115 pKa = 11.84 IDD117 pKa = 3.3 QVLEE121 pKa = 4.33 FKK123 pKa = 11.21 HH124 pKa = 6.2 NVEE127 pKa = 4.35 EE128 pKa = 5.44 LDD130 pKa = 3.69 PALCKK135 pKa = 10.38 AVTVRR140 pKa = 11.84 FVTAYY145 pKa = 10.17 NGKK148 pKa = 9.02 KK149 pKa = 10.14 CEE151 pKa = 3.88 VSFWSSMTPSRR162 pKa = 11.84 RR163 pKa = 11.84 RR164 pKa = 11.84 GAPFFIMYY172 pKa = 7.5 YY173 pKa = 10.87 APLKK177 pKa = 10.76 DD178 pKa = 4.3 GNPPPAMMKK187 pKa = 9.99 WKK189 pKa = 10.27 INLPFKK195 pKa = 10.5 ILNDD199 pKa = 4.0 TEE201 pKa = 4.06 EE202 pKa = 4.6 GPYY205 pKa = 10.27 LSKK208 pKa = 11.23
Molecular weight: 24.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.093
IPC2_protein 7.439
IPC_protein 7.351
Toseland 6.942
ProMoST 8.053
Dawson 8.053
Bjellqvist 8.419
Wikipedia 7.951
Rodwell 8.053
Grimsley 6.956
Solomon 8.141
Lehninger 8.156
Nozaki 8.726
DTASelect 8.156
Thurlkill 8.214
EMBOSS 8.273
Sillero 8.565
Patrickios 4.406
IPC_peptide 8.141
IPC2_peptide 7.454
IPC2.peptide.svr19 7.542
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3682
208
2119
736.4
84.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.726 ± 0.83
2.146 ± 0.23
5.703 ± 0.397
7.74 ± 1.503
4.047 ± 0.61
6.165 ± 0.796
2.499 ± 0.47
7.17 ± 0.596
7.251 ± 1.04
9.533 ± 1.103
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.146 ± 0.152
4.59 ± 0.454
4.237 ± 0.419
3.069 ± 0.331
5.731 ± 0.398
7.414 ± 0.291
4.916 ± 0.395
5.595 ± 0.537
2.01 ± 0.182
3.313 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here