Zooshikella ganghwensis
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5263 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P9VN65|A0A4P9VN65_9GAMM Uncharacterized protein OS=Zooshikella ganghwensis OX=202772 GN=B9G39_10600 PE=4 SV=1
MM1 pKa = 7.29 SRR3 pKa = 11.84 VLIYY7 pKa = 9.11 GVCCFVVGLNSAQAVIIAGDD27 pKa = 3.65 VTGGSAATAGGIFIKK42 pKa = 10.39 LIPPIGDD49 pKa = 3.33 VGDD52 pKa = 5.24 DD53 pKa = 3.74 NFNDD57 pKa = 3.55 PNLYY61 pKa = 10.83 GFDD64 pKa = 3.67 EE65 pKa = 4.81 DD66 pKa = 3.96 QNIIIPAPLTVDD78 pKa = 3.66 IGPSATLPTGTEE90 pKa = 4.09 VASHH94 pKa = 5.51 YY95 pKa = 11.02 VVFDD99 pKa = 3.8 PASSQRR105 pKa = 11.84 MIGYY109 pKa = 9.87 VDD111 pKa = 4.57 FDD113 pKa = 4.21 APILAIMTKK122 pKa = 8.84 TATLAASDD130 pKa = 3.89 FLANVSANYY139 pKa = 9.8 LSPSARR145 pKa = 11.84 GLEE148 pKa = 4.34 SADD151 pKa = 3.29 SVVIDD156 pKa = 3.95 PVV158 pKa = 3.16
Molecular weight: 16.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.706
IPC_protein 3.694
Toseland 3.465
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.719
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.656
Nozaki 3.846
DTASelect 4.151
Thurlkill 3.554
EMBOSS 3.719
Sillero 3.834
Patrickios 0.846
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.738
Protein with the highest isoelectric point:
>tr|A0A4P9VS72|A0A4P9VS72_9GAMM ATP synthase subunit delta OS=Zooshikella ganghwensis OX=202772 GN=atpH PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSCLKK11 pKa = 10.41 RR12 pKa = 11.84 KK13 pKa = 7.77 RR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.34 NGRR28 pKa = 11.84 QILKK32 pKa = 9.88 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.23 GRR39 pKa = 11.84 KK40 pKa = 8.31 QLAAA44 pKa = 4.14
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.369
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5263
0
5263
1647448
23
5975
313.0
35.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.996 ± 0.036
1.166 ± 0.015
5.235 ± 0.034
5.833 ± 0.033
4.031 ± 0.025
6.308 ± 0.04
2.503 ± 0.019
6.557 ± 0.033
5.711 ± 0.032
10.505 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.151 ± 0.019
4.475 ± 0.031
4.187 ± 0.025
5.269 ± 0.04
4.59 ± 0.027
6.562 ± 0.026
5.578 ± 0.035
6.484 ± 0.027
1.392 ± 0.014
3.466 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here