Salinisphaera sp. Q1T1-3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Salinisphaerales; Salinisphaeraceae; Salinisphaera; unclassified Salinisphaera

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3326 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A5JAY8|A0A3A5JAY8_9GAMM Uncharacterized protein OS=Salinisphaera sp. Q1T1-3 OX=2321229 GN=D3260_04135 PE=4 SV=1
MM1 pKa = 6.78PTVVRR6 pKa = 11.84VGRR9 pKa = 11.84GSRR12 pKa = 11.84PLSNTRR18 pKa = 11.84LGATTMIKK26 pKa = 10.3KK27 pKa = 10.01LAIAGALAFTATTAFSAAFADD48 pKa = 3.67MDD50 pKa = 5.04SNNDD54 pKa = 3.11GKK56 pKa = 9.01VTKK59 pKa = 10.18DD60 pKa = 3.12EE61 pKa = 4.37YY62 pKa = 11.05FGTVADD68 pKa = 3.39WGTYY72 pKa = 9.91KK73 pKa = 10.85DD74 pKa = 3.56WDD76 pKa = 4.13TNGDD80 pKa = 3.81GLIEE84 pKa = 4.24ASEE87 pKa = 4.04FDD89 pKa = 3.44AGPFEE94 pKa = 4.88GSYY97 pKa = 9.89TYY99 pKa = 10.38WDD101 pKa = 4.02YY102 pKa = 12.16NNDD105 pKa = 3.6GYY107 pKa = 11.38LDD109 pKa = 3.75SYY111 pKa = 10.86EE112 pKa = 4.6VYY114 pKa = 10.52DD115 pKa = 4.44GLYY118 pKa = 9.27ATYY121 pKa = 10.46DD122 pKa = 3.83ANDD125 pKa = 3.49DD126 pKa = 4.3GYY128 pKa = 10.47WEE130 pKa = 4.18NGEE133 pKa = 3.84WHH135 pKa = 7.26DD136 pKa = 5.39FDD138 pKa = 6.55DD139 pKa = 5.68AGLWDD144 pKa = 3.68

Molecular weight: 15.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A5J190|A0A3A5J190_9GAMM (S)-ureidoglycine aminohydrolase OS=Salinisphaera sp. Q1T1-3 OX=2321229 GN=D3260_14115 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.16RR14 pKa = 11.84THH16 pKa = 5.96GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.97NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.86GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LTPP44 pKa = 3.95

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3326

0

3326

1104782

25

1599

332.2

36.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.223 ± 0.066

0.915 ± 0.013

6.555 ± 0.04

4.908 ± 0.044

3.434 ± 0.027

8.381 ± 0.036

2.469 ± 0.019

4.894 ± 0.027

2.333 ± 0.035

9.967 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.301 ± 0.02

2.61 ± 0.028

4.954 ± 0.028

3.366 ± 0.027

7.648 ± 0.044

5.29 ± 0.031

5.543 ± 0.028

7.297 ± 0.032

1.425 ± 0.018

2.486 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski