Amino acid dipepetide frequency for Salinisphaera sp. Q1T1-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.347AlaAla: 18.347 ± 0.217
1.295AlaCys: 1.295 ± 0.04
9.157AlaAsp: 9.157 ± 0.121
7.51AlaGlu: 7.51 ± 0.113
4.169AlaPhe: 4.169 ± 0.061
11.783AlaGly: 11.783 ± 0.12
3.077AlaHis: 3.077 ± 0.062
6.3AlaIle: 6.3 ± 0.086
2.582AlaLys: 2.582 ± 0.067
14.096AlaLeu: 14.096 ± 0.149
3.21AlaMet: 3.21 ± 0.053
2.932AlaAsn: 2.932 ± 0.06
6.04AlaPro: 6.04 ± 0.085
3.967AlaGln: 3.967 ± 0.063
10.966AlaArg: 10.966 ± 0.128
6.542AlaSer: 6.542 ± 0.087
6.812AlaThr: 6.812 ± 0.086
8.813AlaVal: 8.813 ± 0.094
1.826AlaTrp: 1.826 ± 0.038
2.808AlaTyr: 2.808 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
1.084CysAla: 1.084 ± 0.037
0.14CysCys: 0.14 ± 0.013
0.595CysAsp: 0.595 ± 0.026
0.468CysGlu: 0.468 ± 0.02
0.32CysPhe: 0.32 ± 0.016
0.89CysGly: 0.89 ± 0.028
0.291CysHis: 0.291 ± 0.02
0.387CysIle: 0.387 ± 0.017
0.146CysLys: 0.146 ± 0.011
1.006CysLeu: 1.006 ± 0.03
0.174CysMet: 0.174 ± 0.013
0.208CysAsn: 0.208 ± 0.015
0.496CysPro: 0.496 ± 0.02
0.253CysGln: 0.253 ± 0.014
0.742CysArg: 0.742 ± 0.03
0.422CysSer: 0.422 ± 0.022
0.405CysThr: 0.405 ± 0.022
0.765CysVal: 0.765 ± 0.03
0.12CysTrp: 0.12 ± 0.011
0.243CysTyr: 0.243 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
8.462AspAla: 8.462 ± 0.103
0.53AspCys: 0.53 ± 0.024
4.936AspAsp: 4.936 ± 0.086
3.807AspGlu: 3.807 ± 0.065
2.242AspPhe: 2.242 ± 0.048
5.425AspGly: 5.425 ± 0.074
1.571AspHis: 1.571 ± 0.039
3.221AspIle: 3.221 ± 0.065
1.7AspLys: 1.7 ± 0.05
5.435AspLeu: 5.435 ± 0.078
1.482AspMet: 1.482 ± 0.033
2.029AspAsn: 2.029 ± 0.047
3.065AspPro: 3.065 ± 0.056
2.028AspGln: 2.028 ± 0.043
4.956AspArg: 4.956 ± 0.069
2.846AspSer: 2.846 ± 0.05
3.945AspThr: 3.945 ± 0.064
4.68AspVal: 4.68 ± 0.068
1.221AspTrp: 1.221 ± 0.035
1.967AspTyr: 1.967 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
7.04GluAla: 7.04 ± 0.111
0.369GluCys: 0.369 ± 0.019
2.31GluAsp: 2.31 ± 0.054
1.763GluGlu: 1.763 ± 0.051
1.389GluPhe: 1.389 ± 0.037
2.973GluGly: 2.973 ± 0.052
1.39GluHis: 1.39 ± 0.037
2.931GluIle: 2.931 ± 0.049
1.474GluLys: 1.474 ± 0.045
4.702GluLeu: 4.702 ± 0.084
1.179GluMet: 1.179 ± 0.035
1.38GluAsn: 1.38 ± 0.041
2.379GluPro: 2.379 ± 0.058
2.171GluGln: 2.171 ± 0.049
4.639GluArg: 4.639 ± 0.08
2.807GluSer: 2.807 ± 0.057
3.599GluThr: 3.599 ± 0.068
2.908GluVal: 2.908 ± 0.052
0.563GluTrp: 0.563 ± 0.025
1.115GluTyr: 1.115 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.261PheAla: 4.261 ± 0.068
0.377PheCys: 0.377 ± 0.016
2.817PheAsp: 2.817 ± 0.051
1.879PheGlu: 1.879 ± 0.043
1.312PhePhe: 1.312 ± 0.042
3.438PheGly: 3.438 ± 0.064
0.739PheHis: 0.739 ± 0.03
1.688PheIle: 1.688 ± 0.045
0.862PheLys: 0.862 ± 0.034
2.705PheLeu: 2.705 ± 0.058
0.743PheMet: 0.743 ± 0.03
1.074PheAsn: 1.074 ± 0.035
1.264PhePro: 1.264 ± 0.035
0.903PheGln: 0.903 ± 0.031
1.92PheArg: 1.92 ± 0.043
2.05PheSer: 2.05 ± 0.042
1.837PheThr: 1.837 ± 0.046
2.945PheVal: 2.945 ± 0.061
0.546PheTrp: 0.546 ± 0.024
0.983PheTyr: 0.983 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
9.293GlyAla: 9.293 ± 0.111
0.931GlyCys: 0.931 ± 0.029
5.054GlyAsp: 5.054 ± 0.072
4.122GlyGlu: 4.122 ± 0.059
3.335GlyPhe: 3.335 ± 0.063
6.767GlyGly: 6.767 ± 0.103
2.3GlyHis: 2.3 ± 0.047
4.04GlyIle: 4.04 ± 0.061
2.207GlyLys: 2.207 ± 0.057
9.374GlyLeu: 9.374 ± 0.114
2.331GlyMet: 2.331 ± 0.049
2.12GlyAsn: 2.12 ± 0.048
3.522GlyPro: 3.522 ± 0.062
3.353GlyGln: 3.353 ± 0.057
6.24GlyArg: 6.24 ± 0.082
3.995GlySer: 3.995 ± 0.072
4.453GlyThr: 4.453 ± 0.063
6.377GlyVal: 6.377 ± 0.089
1.466GlyTrp: 1.466 ± 0.042
2.527GlyTyr: 2.527 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
3.07HisAla: 3.07 ± 0.057
0.289HisCys: 0.289 ± 0.018
1.756HisAsp: 1.756 ± 0.043
1.266HisGlu: 1.266 ± 0.038
0.889HisPhe: 0.889 ± 0.026
2.353HisGly: 2.353 ± 0.045
0.741HisHis: 0.741 ± 0.031
1.139HisIle: 1.139 ± 0.033
0.453HisLys: 0.453 ± 0.022
2.283HisLeu: 2.283 ± 0.052
0.537HisMet: 0.537 ± 0.024
0.55HisAsn: 0.55 ± 0.024
1.41HisPro: 1.41 ± 0.037
0.725HisGln: 0.725 ± 0.028
1.976HisArg: 1.976 ± 0.045
0.969HisSer: 0.969 ± 0.032
1.129HisThr: 1.129 ± 0.03
1.914HisVal: 1.914 ± 0.039
0.442HisTrp: 0.442 ± 0.022
0.797HisTyr: 0.797 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
7.398IleAla: 7.398 ± 0.091
0.483IleCys: 0.483 ± 0.017
4.221IleAsp: 4.221 ± 0.055
3.211IleGlu: 3.211 ± 0.062
1.417IlePhe: 1.417 ± 0.04
5.138IleGly: 5.138 ± 0.076
1.041IleHis: 1.041 ± 0.031
1.795IleIle: 1.795 ± 0.045
1.244IleLys: 1.244 ± 0.036
3.332IleLeu: 3.332 ± 0.061
0.858IleMet: 0.858 ± 0.027
1.506IleAsn: 1.506 ± 0.041
1.96IlePro: 1.96 ± 0.042
1.356IleGln: 1.356 ± 0.041
3.107IleArg: 3.107 ± 0.056
2.428IleSer: 2.428 ± 0.047
2.73IleThr: 2.73 ± 0.051
3.994IleVal: 3.994 ± 0.072
0.574IleTrp: 0.574 ± 0.024
1.15IleTyr: 1.15 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
2.943LysAla: 2.943 ± 0.073
0.125LysCys: 0.125 ± 0.011
1.049LysAsp: 1.049 ± 0.034
0.787LysGlu: 0.787 ± 0.033
0.561LysPhe: 0.561 ± 0.023
1.545LysGly: 1.545 ± 0.041
0.589LysHis: 0.589 ± 0.026
1.213LysIle: 1.213 ± 0.039
1.008LysLys: 1.008 ± 0.041
2.267LysLeu: 2.267 ± 0.05
0.468LysMet: 0.468 ± 0.022
0.76LysAsn: 0.76 ± 0.031
1.557LysPro: 1.557 ± 0.043
1.119LysGln: 1.119 ± 0.035
2.145LysArg: 2.145 ± 0.047
1.418LysSer: 1.418 ± 0.046
1.616LysThr: 1.616 ± 0.048
1.351LysVal: 1.351 ± 0.043
0.262LysTrp: 0.262 ± 0.014
0.545LysTyr: 0.545 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
15.198LeuAla: 15.198 ± 0.156
0.963LeuCys: 0.963 ± 0.031
6.702LeuAsp: 6.702 ± 0.09
4.369LeuGlu: 4.369 ± 0.076
3.649LeuPhe: 3.649 ± 0.077
8.445LeuGly: 8.445 ± 0.094
2.047LeuHis: 2.047 ± 0.042
5.06LeuIle: 5.06 ± 0.082
2.399LeuLys: 2.399 ± 0.051
9.09LeuLeu: 9.09 ± 0.135
2.185LeuMet: 2.185 ± 0.049
2.361LeuAsn: 2.361 ± 0.051
5.006LeuPro: 5.006 ± 0.07
2.496LeuGln: 2.496 ± 0.055
6.281LeuArg: 6.281 ± 0.086
6.669LeuSer: 6.669 ± 0.084
5.505LeuThr: 5.505 ± 0.071
7.737LeuVal: 7.737 ± 0.102
1.247LeuTrp: 1.247 ± 0.038
2.264LeuTyr: 2.264 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
3.201MetAla: 3.201 ± 0.057
0.173MetCys: 0.173 ± 0.012
1.08MetAsp: 1.08 ± 0.028
0.75MetGlu: 0.75 ± 0.027
0.676MetPhe: 0.676 ± 0.025
1.599MetGly: 1.599 ± 0.04
0.588MetHis: 0.588 ± 0.02
1.177MetIle: 1.177 ± 0.034
0.678MetLys: 0.678 ± 0.026
2.341MetLeu: 2.341 ± 0.05
0.532MetMet: 0.532 ± 0.024
0.709MetAsn: 0.709 ± 0.025
1.48MetPro: 1.48 ± 0.037
0.925MetGln: 0.925 ± 0.031
1.781MetArg: 1.781 ± 0.044
1.756MetSer: 1.756 ± 0.042
1.649MetThr: 1.649 ± 0.036
1.408MetVal: 1.408 ± 0.041
0.177MetTrp: 0.177 ± 0.012
0.332MetTyr: 0.332 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.376AsnAla: 3.376 ± 0.062
0.194AsnCys: 0.194 ± 0.014
1.726AsnAsp: 1.726 ± 0.044
1.247AsnGlu: 1.247 ± 0.041
0.806AsnPhe: 0.806 ± 0.029
2.379AsnGly: 2.379 ± 0.054
0.558AsnHis: 0.558 ± 0.022
1.204AsnIle: 1.204 ± 0.033
0.657AsnLys: 0.657 ± 0.024
2.432AsnLeu: 2.432 ± 0.053
0.546AsnMet: 0.546 ± 0.021
0.801AsnAsn: 0.801 ± 0.032
1.613AsnPro: 1.613 ± 0.04
0.969AsnGln: 0.969 ± 0.033
1.972AsnArg: 1.972 ± 0.049
1.127AsnSer: 1.127 ± 0.033
1.569AsnThr: 1.569 ± 0.042
1.894AsnVal: 1.894 ± 0.043
0.385AsnTrp: 0.385 ± 0.02
0.649AsnTyr: 0.649 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
6.859ProAla: 6.859 ± 0.091
0.323ProCys: 0.323 ± 0.017
3.908ProAsp: 3.908 ± 0.068
2.897ProGlu: 2.897 ± 0.06
1.561ProPhe: 1.561 ± 0.038
4.49ProGly: 4.49 ± 0.079
1.074ProHis: 1.074 ± 0.033
2.345ProIle: 2.345 ± 0.046
1.13ProLys: 1.13 ± 0.035
4.302ProLeu: 4.302 ± 0.063
1.239ProMet: 1.239 ± 0.038
1.298ProAsn: 1.298 ± 0.035
2.254ProPro: 2.254 ± 0.052
1.475ProGln: 1.475 ± 0.036
3.272ProArg: 3.272 ± 0.065
2.706ProSer: 2.706 ± 0.043
2.594ProThr: 2.594 ± 0.052
3.908ProVal: 3.908 ± 0.064
0.759ProTrp: 0.759 ± 0.024
1.141ProTyr: 1.141 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.826GlnAla: 4.826 ± 0.077
0.267GlnCys: 0.267 ± 0.015
1.492GlnAsp: 1.492 ± 0.039
0.999GlnGlu: 0.999 ± 0.033
1.081GlnPhe: 1.081 ± 0.034
2.41GlnGly: 2.41 ± 0.046
0.832GlnHis: 0.832 ± 0.027
1.829GlnIle: 1.829 ± 0.044
0.835GlnLys: 0.835 ± 0.036
3.364GlnLeu: 3.364 ± 0.055
0.72GlnMet: 0.72 ± 0.025
0.865GlnAsn: 0.865 ± 0.035
1.902GlnPro: 1.902 ± 0.049
1.543GlnGln: 1.543 ± 0.053
3.1GlnArg: 3.1 ± 0.066
2.016GlnSer: 2.016 ± 0.05
2.079GlnThr: 2.079 ± 0.043
2.172GlnVal: 2.172 ± 0.044
0.488GlnTrp: 0.488 ± 0.02
0.843GlnTyr: 0.843 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
8.992ArgAla: 8.992 ± 0.117
0.726ArgCys: 0.726 ± 0.03
4.837ArgAsp: 4.837 ± 0.074
3.978ArgGlu: 3.978 ± 0.073
2.968ArgPhe: 2.968 ± 0.057
5.093ArgGly: 5.093 ± 0.078
2.324ArgHis: 2.324 ± 0.05
4.074ArgIle: 4.074 ± 0.066
1.489ArgLys: 1.489 ± 0.044
9.172ArgLeu: 9.172 ± 0.111
1.849ArgMet: 1.849 ± 0.038
1.737ArgAsn: 1.737 ± 0.047
3.609ArgPro: 3.609 ± 0.062
3.023ArgGln: 3.023 ± 0.061
6.904ArgArg: 6.904 ± 0.105
3.3ArgSer: 3.3 ± 0.057
3.383ArgThr: 3.383 ± 0.057
5.466ArgVal: 5.466 ± 0.073
1.245ArgTrp: 1.245 ± 0.035
2.308ArgTyr: 2.308 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
6.9SerAla: 6.9 ± 0.076
0.418SerCys: 0.418 ± 0.023
3.282SerAsp: 3.282 ± 0.066
2.426SerGlu: 2.426 ± 0.05
1.794SerPhe: 1.794 ± 0.041
5.053SerGly: 5.053 ± 0.083
1.237SerHis: 1.237 ± 0.037
2.44SerIle: 2.44 ± 0.053
1.193SerLys: 1.193 ± 0.045
5.157SerLeu: 5.157 ± 0.078
1.285SerMet: 1.285 ± 0.035
1.424SerAsn: 1.424 ± 0.041
2.79SerPro: 2.79 ± 0.055
1.955SerGln: 1.955 ± 0.045
4.123SerArg: 4.123 ± 0.065
2.973SerSer: 2.973 ± 0.071
2.729SerThr: 2.729 ± 0.057
3.778SerVal: 3.778 ± 0.056
0.729SerTrp: 0.729 ± 0.024
1.217SerTyr: 1.217 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
6.806ThrAla: 6.806 ± 0.089
0.397ThrCys: 0.397 ± 0.018
3.526ThrAsp: 3.526 ± 0.064
2.496ThrGlu: 2.496 ± 0.046
1.723ThrPhe: 1.723 ± 0.039
5.167ThrGly: 5.167 ± 0.067
1.443ThrHis: 1.443 ± 0.035
2.553ThrIle: 2.553 ± 0.053
0.978ThrLys: 0.978 ± 0.038
6.6ThrLeu: 6.6 ± 0.069
1.111ThrMet: 1.111 ± 0.034
1.314ThrAsn: 1.314 ± 0.034
3.65ThrPro: 3.65 ± 0.058
1.706ThrGln: 1.706 ± 0.045
4.175ThrArg: 4.175 ± 0.075
2.629ThrSer: 2.629 ± 0.051
3.191ThrThr: 3.191 ± 0.059
4.104ThrVal: 4.104 ± 0.067
0.646ThrTrp: 0.646 ± 0.026
1.215ThrTyr: 1.215 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
10.006ValAla: 10.006 ± 0.101
0.767ValCys: 0.767 ± 0.026
4.803ValAsp: 4.803 ± 0.071
3.574ValGlu: 3.574 ± 0.059
2.872ValPhe: 2.872 ± 0.046
5.664ValGly: 5.664 ± 0.077
1.69ValHis: 1.69 ± 0.049
3.885ValIle: 3.885 ± 0.062
1.44ValLys: 1.44 ± 0.042
7.301ValLeu: 7.301 ± 0.098
1.754ValMet: 1.754 ± 0.04
1.937ValAsn: 1.937 ± 0.048
3.571ValPro: 3.571 ± 0.065
1.861ValGln: 1.861 ± 0.048
4.61ValArg: 4.61 ± 0.061
4.292ValSer: 4.292 ± 0.067
4.28ValThr: 4.28 ± 0.06
6.041ValVal: 6.041 ± 0.089
0.9ValTrp: 0.9 ± 0.03
1.722ValTyr: 1.722 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
1.403TrpAla: 1.403 ± 0.034
0.15TrpCys: 0.15 ± 0.011
0.549TrpAsp: 0.549 ± 0.025
0.37TrpGlu: 0.37 ± 0.018
0.575TrpPhe: 0.575 ± 0.019
0.894TrpGly: 0.894 ± 0.026
0.549TrpHis: 0.549 ± 0.021
0.692TrpIle: 0.692 ± 0.025
0.265TrpLys: 0.265 ± 0.015
2.191TrpLeu: 2.191 ± 0.05
0.346TrpMet: 0.346 ± 0.017
0.362TrpAsn: 0.362 ± 0.018
0.821TrpPro: 0.821 ± 0.028
0.905TrpGln: 0.905 ± 0.031
1.315TrpArg: 1.315 ± 0.036
0.747TrpSer: 0.747 ± 0.027
0.635TrpThr: 0.635 ± 0.027
0.934TrpVal: 0.934 ± 0.028
0.242TrpTrp: 0.242 ± 0.015
0.306TrpTyr: 0.306 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.767TyrAla: 2.767 ± 0.055
0.237TyrCys: 0.237 ± 0.014
1.747TyrAsp: 1.747 ± 0.043
1.16TyrGlu: 1.16 ± 0.035
1.003TyrPhe: 1.003 ± 0.03
2.3TyrGly: 2.3 ± 0.052
0.607TyrHis: 0.607 ± 0.026
0.97TyrIle: 0.97 ± 0.026
0.593TyrLys: 0.593 ± 0.028
2.522TyrLeu: 2.522 ± 0.047
0.463TyrMet: 0.463 ± 0.023
0.734TyrAsn: 0.734 ± 0.029
1.152TyrPro: 1.152 ± 0.038
0.929TyrGln: 0.929 ± 0.03
2.253TyrArg: 2.253 ± 0.05
1.21TyrSer: 1.21 ± 0.041
1.293TyrThr: 1.293 ± 0.038
1.778TyrVal: 1.778 ± 0.048
0.415TyrTrp: 0.415 ± 0.02
0.726TyrTyr: 0.726 ± 0.032
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3326 proteins (1104782 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski