Delftia phage RG-2014

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Dendoorenvirus; Delftia virus RG2014

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097PBE4|A0A097PBE4_9CAUD Uncharacterized protein OS=Delftia phage RG-2014 OX=1563661 GN=RG2014_087 PE=4 SV=1
MM1 pKa = 7.29SVISVIPEE9 pKa = 3.76PDD11 pKa = 3.32FNAQKK16 pKa = 10.94AGIRR20 pKa = 11.84QLQAEE25 pKa = 4.98LAAEE29 pKa = 4.08MSDD32 pKa = 3.91VIVGAQTTQYY42 pKa = 11.22GPDD45 pKa = 3.49EE46 pKa = 4.36YY47 pKa = 10.82TFEE50 pKa = 5.24PGLGQIHH57 pKa = 5.42YY58 pKa = 7.68TAATANKK65 pKa = 9.3IGKK68 pKa = 9.19DD69 pKa = 3.44LGASLAAALGNVYY82 pKa = 10.19PNPPTLKK89 pKa = 10.29PNPDD93 pKa = 4.36DD94 pKa = 3.38YY95 pKa = 11.48WFMGEE100 pKa = 5.29LYY102 pKa = 10.22PEE104 pKa = 4.86ADD106 pKa = 3.74FEE108 pKa = 4.62TEE110 pKa = 4.07PVNVATMDD118 pKa = 3.43YY119 pKa = 10.44YY120 pKa = 11.25RR121 pKa = 11.84RR122 pKa = 11.84FLKK125 pKa = 10.51LLHH128 pKa = 5.86EE129 pKa = 4.64RR130 pKa = 11.84EE131 pKa = 4.35FKK133 pKa = 10.6FVNSVAYY140 pKa = 9.7EE141 pKa = 3.84ILDD144 pKa = 3.85FFHH147 pKa = 7.32PEE149 pKa = 3.04AWKK152 pKa = 10.21QRR154 pKa = 11.84NFLGNPGLSGWYY166 pKa = 8.69PPSAFIQPTNFDD178 pKa = 3.55ALNYY182 pKa = 9.51ISAVQAQILRR192 pKa = 11.84EE193 pKa = 3.98AVALGMEE200 pKa = 4.56PLFQIGEE207 pKa = 4.2PWWWDD212 pKa = 3.02GSYY215 pKa = 9.24STGEE219 pKa = 4.22GKK221 pKa = 9.6NAPCLYY227 pKa = 10.64DD228 pKa = 3.61PATMVLYY235 pKa = 10.11EE236 pKa = 4.81AEE238 pKa = 4.38TGNQVPTPYY247 pKa = 9.63IKK249 pKa = 10.76SIFDD253 pKa = 3.75PVASNQWPYY262 pKa = 11.39VDD264 pKa = 3.79WLRR267 pKa = 11.84KK268 pKa = 9.67KK269 pKa = 10.91LGDD272 pKa = 3.4STNYY276 pKa = 9.35IRR278 pKa = 11.84DD279 pKa = 3.65RR280 pKa = 11.84VKK282 pKa = 11.09SLIPGSKK289 pKa = 8.97ATLLFFTPQIMSPASEE305 pKa = 3.76LTYY308 pKa = 10.89RR309 pKa = 11.84LNFPIAEE316 pKa = 3.94WSYY319 pKa = 9.95PNYY322 pKa = 10.79DD323 pKa = 3.16FVQIEE328 pKa = 4.35DD329 pKa = 4.13YY330 pKa = 11.26DD331 pKa = 3.96WIIAGDD337 pKa = 3.6IEE339 pKa = 5.1LVPKK343 pKa = 10.35TFDD346 pKa = 3.65AATNLLNYY354 pKa = 9.01PLSEE358 pKa = 3.63VHH360 pKa = 5.8YY361 pKa = 10.18FIGFVLKK368 pKa = 11.07AEE370 pKa = 4.57DD371 pKa = 3.57AYY373 pKa = 10.02IWRR376 pKa = 11.84TCDD379 pKa = 3.05QALGLAIEE387 pKa = 4.08AQIPYY392 pKa = 9.83KK393 pKa = 10.3YY394 pKa = 9.62LWSYY398 pKa = 6.96TQVMRR403 pKa = 11.84DD404 pKa = 3.74NIVMQRR410 pKa = 11.84LRR412 pKa = 11.84DD413 pKa = 3.88CGCC416 pKa = 3.68

Molecular weight: 47.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097PAK7|A0A097PAK7_9CAUD Uncharacterized protein OS=Delftia phage RG-2014 OX=1563661 GN=RG2014_011 PE=4 SV=1
MM1 pKa = 7.17AQFYY5 pKa = 8.27VQPLHH10 pKa = 6.38NPNSGEE16 pKa = 3.83FCTVLRR22 pKa = 11.84QMRR25 pKa = 11.84HH26 pKa = 5.06LSMLGITVPKK36 pKa = 8.18TLKK39 pKa = 10.26VLATGRR45 pKa = 11.84VEE47 pKa = 5.24GYY49 pKa = 10.52QYY51 pKa = 10.08TCPSSRR57 pKa = 11.84RR58 pKa = 11.84QAQINEE64 pKa = 4.59TPVHH68 pKa = 5.98MGRR71 pKa = 11.84FSIEE75 pKa = 3.51RR76 pKa = 11.84VQTRR80 pKa = 11.84KK81 pKa = 8.84GTWLRR86 pKa = 11.84YY87 pKa = 10.24VNIAWGGEE95 pKa = 3.59IHH97 pKa = 7.1RR98 pKa = 11.84VQIQPTTLFIDD109 pKa = 3.93

Molecular weight: 12.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

23589

44

3413

268.1

29.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.17 ± 0.745

0.945 ± 0.135

5.918 ± 0.13

6.066 ± 0.17

3.599 ± 0.174

7.092 ± 0.245

2.022 ± 0.174

4.812 ± 0.226

5.494 ± 0.153

8.474 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.345 ± 0.107

4.239 ± 0.174

4.752 ± 0.205

4.943 ± 0.236

5.32 ± 0.199

5.193 ± 0.142

5.558 ± 0.197

6.291 ± 0.247

1.662 ± 0.178

3.103 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski