Maribius salinus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae;

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6AW90|A0A1M6AW90_9RHOB Phosphate transport system permease protein PstA OS=Maribius salinus OX=313368 GN=SAMN04488012_101277 PE=3 SV=1
MM1 pKa = 7.49RR2 pKa = 11.84HH3 pKa = 6.09ILLASAATMALGSGMAAAQEE23 pKa = 4.2LVFPVGEE30 pKa = 4.82GAFSWDD36 pKa = 3.34SYY38 pKa = 11.81EE39 pKa = 4.85EE40 pKa = 3.76FAANNDD46 pKa = 3.73YY47 pKa = 11.13SGEE50 pKa = 4.24TIDD53 pKa = 5.87IAGPWTGADD62 pKa = 3.4AEE64 pKa = 4.63LMNSVAAYY72 pKa = 9.27FAEE75 pKa = 4.37ATGATVNYY83 pKa = 9.53SGSDD87 pKa = 3.17SFEE90 pKa = 3.74QDD92 pKa = 2.53IVISTEE98 pKa = 3.9ANSAPNLAAFPQPGLVADD116 pKa = 4.6LASRR120 pKa = 11.84GSITPLDD127 pKa = 3.92EE128 pKa = 3.96EE129 pKa = 4.45TASFIAEE136 pKa = 4.01NYY138 pKa = 9.35SAGEE142 pKa = 4.11SWVDD146 pKa = 3.24LATFEE151 pKa = 5.03GPDD154 pKa = 4.01GEE156 pKa = 4.52SAMYY160 pKa = 10.18AFPYY164 pKa = 10.04KK165 pKa = 9.83IDD167 pKa = 3.59VKK169 pKa = 10.88SLVWYY174 pKa = 10.32SPEE177 pKa = 3.72QFEE180 pKa = 4.29EE181 pKa = 3.94AGYY184 pKa = 9.41EE185 pKa = 3.82IPEE188 pKa = 4.27TYY190 pKa = 10.47EE191 pKa = 3.66EE192 pKa = 4.37LKK194 pKa = 11.01EE195 pKa = 3.88LTAQIAEE202 pKa = 4.54DD203 pKa = 5.67GVTPWCIGLGSGAATGWPATDD224 pKa = 3.1WVEE227 pKa = 5.75DD228 pKa = 3.29IMLRR232 pKa = 11.84TATPEE237 pKa = 3.91QYY239 pKa = 11.2DD240 pKa = 3.36NWVTNDD246 pKa = 3.72MPFDD250 pKa = 4.51DD251 pKa = 4.86PVVVAAIEE259 pKa = 4.28EE260 pKa = 4.62YY261 pKa = 9.86GTFLQEE267 pKa = 4.36GYY269 pKa = 11.18VNGGRR274 pKa = 11.84EE275 pKa = 3.93AAATTDD281 pKa = 4.18FRR283 pKa = 11.84DD284 pKa = 3.76SPSGLFQFPPEE295 pKa = 4.47CYY297 pKa = 8.14MHH299 pKa = 6.63KK300 pKa = 10.01QATFIPTFFPEE311 pKa = 4.4GTVVGEE317 pKa = 4.55DD318 pKa = 2.7VDD320 pKa = 5.26FFYY323 pKa = 11.09FPAPEE328 pKa = 4.84GGEE331 pKa = 4.26TPVLGGGTMFAITEE345 pKa = 4.11DD346 pKa = 3.91SEE348 pKa = 4.45TARR351 pKa = 11.84GFIDD355 pKa = 4.16FLKK358 pKa = 10.23TPIAHH363 pKa = 6.97EE364 pKa = 3.56IWAAQGGLLSPLTSINPEE382 pKa = 3.99VFPSDD387 pKa = 3.33PARR390 pKa = 11.84ATNDD394 pKa = 2.82ILLNATTFRR403 pKa = 11.84FDD405 pKa = 4.8GSDD408 pKa = 3.54LMPGEE413 pKa = 4.38IGAGAFWTQMVEE425 pKa = 4.38FTTGTQDD432 pKa = 3.95AEE434 pKa = 4.3ATATAIQEE442 pKa = 3.94RR443 pKa = 11.84WDD445 pKa = 3.54NLKK448 pKa = 10.6NN449 pKa = 3.5

Molecular weight: 48.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6GTN6|A0A1M6GTN6_9RHOB TVP38/TMEM64 family membrane protein OS=Maribius salinus OX=313368 GN=SAMN04488012_10584 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.83HH14 pKa = 4.64RR15 pKa = 11.84HH16 pKa = 3.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3413

0

3413

1074527

39

9857

314.8

33.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.856 ± 0.065

0.83 ± 0.018

6.868 ± 0.058

5.623 ± 0.04

3.575 ± 0.028

9.194 ± 0.071

1.969 ± 0.026

4.821 ± 0.033

2.574 ± 0.036

9.994 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.032

2.327 ± 0.025

5.248 ± 0.05

2.995 ± 0.025

7.161 ± 0.061

4.911 ± 0.04

5.532 ± 0.051

7.373 ± 0.04

1.415 ± 0.02

2.058 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski