Aeromicrobium choanae
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3277 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4Z1V5|A0A1T4Z1V5_9ACTN Uncharacterized protein OS=Aeromicrobium choanae OX=1736691 GN=SAMN06295964_1723 PE=4 SV=1
MM1 pKa = 7.97 RR2 pKa = 11.84 VRR4 pKa = 11.84 LLLLSALVLPILAACGGGDD23 pKa = 4.39 DD24 pKa = 5.61 DD25 pKa = 4.78 SASSSGEE32 pKa = 3.67 CTYY35 pKa = 11.36 AEE37 pKa = 4.46 EE38 pKa = 4.89 GSAAKK43 pKa = 10.5 DD44 pKa = 3.06 ADD46 pKa = 4.13 LPPAEE51 pKa = 4.66 LLAPEE56 pKa = 4.46 TLTMKK61 pKa = 9.91 TNRR64 pKa = 11.84 GTFALTLDD72 pKa = 4.42 AEE74 pKa = 4.53 AAPCTVNSFASLAEE88 pKa = 3.66 QGYY91 pKa = 10.37 FDD93 pKa = 3.37 GTKK96 pKa = 9.91 CHH98 pKa = 6.59 RR99 pKa = 11.84 LVPGFVLQCGDD110 pKa = 3.84 PSASGTGGPGYY121 pKa = 8.93 TFEE124 pKa = 6.26 DD125 pKa = 4.39 EE126 pKa = 4.52 LSGDD130 pKa = 3.53 EE131 pKa = 4.43 TYY133 pKa = 10.53 PAGTLAMANSGPDD146 pKa = 3.41 TNGSQFFIVLDD157 pKa = 4.15 DD158 pKa = 4.58 AEE160 pKa = 5.07 LPAAYY165 pKa = 8.55 TVFGTVDD172 pKa = 3.3 EE173 pKa = 5.36 AGLEE177 pKa = 4.05 AARR180 pKa = 11.84 AIAAEE185 pKa = 4.32 GNGPDD190 pKa = 3.72 GVAPKK195 pKa = 10.17 SDD197 pKa = 3.47 VVIEE201 pKa = 4.34 SVSS204 pKa = 3.07
Molecular weight: 20.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.579
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.617
Grimsley 3.49
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.088
Thurlkill 3.63
EMBOSS 3.694
Sillero 3.91
Patrickios 1.863
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A1T4YVY6|A0A1T4YVY6_9ACTN Solute carrier family 10 (Sodium/bile acid cotransporter) member 7 OS=Aeromicrobium choanae OX=1736691 GN=SAMN06295964_1096 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.56 KK16 pKa = 9.27 HH17 pKa = 5.36 RR18 pKa = 11.84 KK19 pKa = 5.5 MLKK22 pKa = 7.41 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 LGKK33 pKa = 10.04
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3277
0
3277
1069643
30
2996
326.4
35.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.824 ± 0.053
0.629 ± 0.009
6.509 ± 0.035
5.946 ± 0.043
3.007 ± 0.026
8.973 ± 0.036
2.137 ± 0.023
3.995 ± 0.033
2.134 ± 0.036
10.075 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.889 ± 0.019
1.82 ± 0.022
5.367 ± 0.027
2.711 ± 0.024
7.456 ± 0.049
5.451 ± 0.028
6.219 ± 0.048
9.445 ± 0.042
1.528 ± 0.017
1.884 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here