Halomonas xianhensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4043 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3G3R2|A0A1I3G3R2_9GAMM Transcriptional regulator IclR family OS=Halomonas xianhensis OX=442341 GN=SAMN04487959_12512 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.47KK3 pKa = 8.85STTGLLLGSALVVGLSGCANSGAQSTSSYY32 pKa = 10.94GSEE35 pKa = 3.34QDD37 pKa = 3.01AWYY40 pKa = 10.2QSPFVCGLAGGLIGGGIGYY59 pKa = 7.66ATSSEE64 pKa = 3.99SDD66 pKa = 3.45EE67 pKa = 5.23DD68 pKa = 3.99DD69 pKa = 3.95GAALGSVAGATAGALLCADD88 pKa = 5.19FSSQIGDD95 pKa = 3.38SDD97 pKa = 4.36GDD99 pKa = 4.01GVPDD103 pKa = 6.13DD104 pKa = 5.8RR105 pKa = 11.84DD106 pKa = 3.43QCPNTPAGVAVDD118 pKa = 4.77ASGCPLDD125 pKa = 3.8TDD127 pKa = 4.12GDD129 pKa = 4.39GVPDD133 pKa = 4.78YY134 pKa = 10.64MDD136 pKa = 4.17EE137 pKa = 4.86CPGTPAGVEE146 pKa = 4.39VNASGCPLDD155 pKa = 3.97SDD157 pKa = 4.3GDD159 pKa = 4.2GVPDD163 pKa = 5.59YY164 pKa = 10.71MDD166 pKa = 3.24QCPNTPAGAEE176 pKa = 4.15VNALGCEE183 pKa = 4.01ADD185 pKa = 4.57VVLQDD190 pKa = 3.65VNFEE194 pKa = 4.03FDD196 pKa = 3.53SAQLTPGAEE205 pKa = 4.0RR206 pKa = 11.84ILDD209 pKa = 3.69GVAQKK214 pKa = 10.72LSANEE219 pKa = 3.82NVRR222 pKa = 11.84VRR224 pKa = 11.84LEE226 pKa = 3.79GHH228 pKa = 5.84TDD230 pKa = 3.26SVGSDD235 pKa = 3.59SYY237 pKa = 12.07NKK239 pKa = 10.25DD240 pKa = 2.88LSQRR244 pKa = 11.84RR245 pKa = 11.84ANSVKK250 pKa = 10.51DD251 pKa = 3.54YY252 pKa = 9.36LTSQGIAASRR262 pKa = 11.84MQAIGYY268 pKa = 9.73GEE270 pKa = 4.31EE271 pKa = 4.05QPIATNEE278 pKa = 4.04TAAGRR283 pKa = 11.84AQNRR287 pKa = 11.84RR288 pKa = 11.84VEE290 pKa = 4.12LGEE293 pKa = 3.94WQQQ296 pKa = 3.26

Molecular weight: 30.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3BIG8|A0A1I3BIG8_9GAMM Twitching motility protein PilU OS=Halomonas xianhensis OX=442341 GN=SAMN04487959_106269 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.42NGRR28 pKa = 11.84QVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4043

0

4043

1302872

39

4373

322.3

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.923 ± 0.049

0.959 ± 0.013

5.815 ± 0.033

6.359 ± 0.038

3.465 ± 0.023

8.077 ± 0.033

2.476 ± 0.021

4.801 ± 0.03

2.73 ± 0.034

11.345 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.018

2.653 ± 0.023

4.916 ± 0.023

4.031 ± 0.028

7.221 ± 0.051

5.516 ± 0.025

5.047 ± 0.028

7.133 ± 0.031

1.507 ± 0.017

2.497 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski