Black medic leaf roll virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Mulpavirales; Nanoviridae; Nanovirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9TSN2|V9TSN2_9VIRU U4 protein OS=Black medic leaf roll virus OX=2038729 GN=U4 PE=4 SV=1
MM1 pKa = 7.4GLRR4 pKa = 11.84YY5 pKa = 9.6FSVLPEE11 pKa = 3.9EE12 pKa = 4.45LKK14 pKa = 10.46EE15 pKa = 4.25KK16 pKa = 10.26IVRR19 pKa = 11.84EE20 pKa = 3.97HH21 pKa = 5.5MRR23 pKa = 11.84EE24 pKa = 3.72EE25 pKa = 4.15RR26 pKa = 11.84KK27 pKa = 8.33TEE29 pKa = 3.86FLEE32 pKa = 4.27KK33 pKa = 10.26AIEE36 pKa = 4.38DD37 pKa = 3.48SCRR40 pKa = 11.84RR41 pKa = 11.84YY42 pKa = 9.76EE43 pKa = 4.44AFITEE48 pKa = 4.17DD49 pKa = 3.3PSYY52 pKa = 11.47EE53 pKa = 4.06DD54 pKa = 4.85LLSLSKK60 pKa = 10.74FLDD63 pKa = 3.39SLVNYY68 pKa = 10.24VGDD71 pKa = 4.16KK72 pKa = 11.27LNTRR76 pKa = 11.84CLIKK80 pKa = 9.83WKK82 pKa = 10.26KK83 pKa = 9.98DD84 pKa = 3.16VPVKK88 pKa = 10.53VKK90 pKa = 10.45YY91 pKa = 10.6GVMEE95 pKa = 4.06EE96 pKa = 3.96QHH98 pKa = 5.73IQLYY102 pKa = 10.34GPLDD106 pKa = 4.09MEE108 pKa = 5.99DD109 pKa = 3.81MLCGEE114 pKa = 4.76LLLPEE119 pKa = 4.78EE120 pKa = 5.2DD121 pKa = 4.41EE122 pKa = 6.18DD123 pKa = 6.44DD124 pKa = 3.35ITYY127 pKa = 9.9EE128 pKa = 4.05DD129 pKa = 4.44GMIVHH134 pKa = 7.22CSQLDD139 pKa = 3.57RR140 pKa = 11.84LFSDD144 pKa = 3.87LGINVTYY151 pKa = 9.29ITVSNKK157 pKa = 10.12CICTPLNKK165 pKa = 9.95EE166 pKa = 4.17VVIVV170 pKa = 3.68

Molecular weight: 19.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9TN96|V9TN96_9VIRU U2 protein OS=Black medic leaf roll virus OX=2038729 GN=U2 PE=4 SV=1
MM1 pKa = 7.37VRR3 pKa = 11.84TNWPMRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84AVGKK15 pKa = 8.65RR16 pKa = 11.84SSRR19 pKa = 11.84RR20 pKa = 11.84GYY22 pKa = 10.64GRR24 pKa = 11.84FYY26 pKa = 10.78PNTPTSRR33 pKa = 11.84VLYY36 pKa = 9.92HH37 pKa = 6.85SSAVLQKK44 pKa = 11.16DD45 pKa = 3.93SITGTEE51 pKa = 3.92IKK53 pKa = 10.24PDD55 pKa = 3.88GEE57 pKa = 4.03VGRR60 pKa = 11.84YY61 pKa = 6.96NMKK64 pKa = 10.46RR65 pKa = 11.84IMLTCTVKK73 pKa = 10.47IPPGEE78 pKa = 3.96LMNFLVVKK86 pKa = 10.65SSSPLVNWANLFTTPALLVKK106 pKa = 10.59EE107 pKa = 4.66SAQDD111 pKa = 3.44MVSIIAKK118 pKa = 10.07GKK120 pKa = 9.91IEE122 pKa = 4.07SSGAGVSEE130 pKa = 4.3VTKK133 pKa = 10.89SFNKK137 pKa = 9.48FVKK140 pKa = 10.2LGSGVSQTQHH150 pKa = 6.36VYY152 pKa = 10.6IIMYY156 pKa = 8.44TSASAKK162 pKa = 9.71VVLEE166 pKa = 3.41HH167 pKa = 7.06RR168 pKa = 11.84MYY170 pKa = 10.79IEE172 pKa = 3.9VV173 pKa = 3.44

Molecular weight: 19.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1289

109

286

161.1

18.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.491 ± 0.885

2.483 ± 0.515

6.206 ± 0.791

7.292 ± 0.852

3.569 ± 0.508

6.129 ± 0.624

1.784 ± 0.393

6.594 ± 0.77

7.06 ± 0.54

8.844 ± 0.885

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.258 ± 0.381

3.801 ± 0.366

3.026 ± 0.405

3.103 ± 0.364

6.439 ± 0.428

7.525 ± 0.867

4.5 ± 0.674

8.301 ± 0.611

1.396 ± 0.238

5.198 ± 0.466
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski