Hubei tombus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH26|A0A1L3KH26_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 1 OX=1923255 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 5.66PNAEE6 pKa = 4.1LVNTPVTYY14 pKa = 10.4PDD16 pKa = 4.08DD17 pKa = 4.15TPVGIPFQNSRR28 pKa = 11.84VSTRR32 pKa = 11.84DD33 pKa = 3.14AGDD36 pKa = 3.83DD37 pKa = 3.5GMTGGTEE44 pKa = 4.26TVSGSPAPTTQPVQTATLPDD64 pKa = 3.67GNYY67 pKa = 9.39TLQNYY72 pKa = 7.55EE73 pKa = 3.95GNNVVNNLYY82 pKa = 10.2HH83 pKa = 6.41QLLKK87 pKa = 10.98FGGEE91 pKa = 4.24TYY93 pKa = 9.75QWQWTNAPVSSPYY106 pKa = 9.84PGYY109 pKa = 11.19VYY111 pKa = 9.71GTLYY115 pKa = 10.62QPSTPHH121 pKa = 5.77GAGWLYY127 pKa = 10.86MNDD130 pKa = 3.53SDD132 pKa = 5.25GGPSFGDD139 pKa = 3.2TAAYY143 pKa = 10.19VCLKK147 pKa = 10.28PVFEE151 pKa = 4.98GASQVTTWILATPGGKK167 pKa = 9.84LGACDD172 pKa = 3.88KK173 pKa = 10.47SSSYY177 pKa = 11.41GGIANGIGYY186 pKa = 10.21YY187 pKa = 10.0MNFDD191 pKa = 3.77NNSPRR196 pKa = 11.84KK197 pKa = 8.78WSFTKK202 pKa = 10.23MSS204 pKa = 3.42

Molecular weight: 21.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGL7|A0A1L3KGL7_9VIRU Capsid protein OS=Hubei tombus-like virus 1 OX=1923255 PE=3 SV=1
MM1 pKa = 7.94PYY3 pKa = 8.81VTMYY7 pKa = 10.81SPVVVAGSNTHH18 pKa = 6.37HH19 pKa = 6.29FHH21 pKa = 6.56HH22 pKa = 7.35RR23 pKa = 11.84STRR26 pKa = 11.84FYY28 pKa = 10.74FSQLHH33 pKa = 5.22STHH36 pKa = 6.39SLWPRR41 pKa = 11.84QRR43 pKa = 11.84ISRR46 pKa = 11.84NQDD49 pKa = 2.58NANRR53 pKa = 11.84RR54 pKa = 11.84ATARR58 pKa = 11.84MWSSVPRR65 pKa = 11.84LRR67 pKa = 11.84RR68 pKa = 11.84VPEE71 pKa = 3.87EE72 pKa = 3.81PPRR75 pKa = 11.84ILAQDD80 pKa = 3.62RR81 pKa = 11.84PSCFSTSGKK90 pKa = 10.34APSAATCCQPTFHH103 pKa = 7.15GFRR106 pKa = 11.84EE107 pKa = 4.47SPQASRR113 pKa = 11.84GGASTTSRR121 pKa = 11.84SGTSPEE127 pKa = 4.01CPPRR131 pKa = 11.84RR132 pKa = 11.84TEE134 pKa = 3.62QQ135 pKa = 3.01

Molecular weight: 15.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1370

135

822

342.5

38.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.934 ± 0.362

2.117 ± 0.483

5.109 ± 0.582

4.964 ± 1.471

3.577 ± 0.39

7.81 ± 1.161

1.606 ± 0.582

3.212 ± 0.481

5.255 ± 1.097

6.934 ± 0.78

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.482 ± 0.442

4.672 ± 0.901

6.642 ± 0.969

3.431 ± 0.931

7.445 ± 1.722

7.518 ± 1.638

6.642 ± 1.368

7.664 ± 0.785

2.117 ± 0.139

3.796 ± 0.779
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski